Search Results (283)

The Tsushima leopard cat (Prionailurus bengalensis euptilurus), an endangered feline endemic to Tsushima Island, Japan, faces critical threats due to its small and isolated population. Understanding its demographic history and genetic differentiation from continental populations is essential for conservation planning. In this study, we performed whole-genome sequencing of four Tsushima individuals and applied demographic inference methods, including pairwise sequentially Markovian coalescent (PSMC) and Sequentially Markovian Coalescent (SMC++), to reconstruct the historical effective population size (Ne) and estimate divergence times. PSMC revealed a population expansion between 200,000 and 100,000 years ago, followed by a long-term decline. SMC++ inferred a continuous decline and estimated that the divergence from the Korean leopard cat population occurred approximately 30,000–20,000 years ago. Genetic diversity analysis showed that the Tsushima population has significantly lower heterozygosity and higher inbreeding levels than continental populations. Genetic clustering based on genome-wide SNPs indicated that the Tsushima population is genetically closest to the Korean population, forming a northern cluster distinct from southern populations, such as Borneo and the Malay Peninsula. These findings provide valuable insights into the evolutionary history and genetic status of the Tsushima leopard cat and contribute critical data for the design of future conservation strategies targeting this unique insular lineage. Full article

Background/Objectives: Childhood stunting remains a critical public health issue in low- and middle-income countries. Despite Malaysia’s economic growth, there is limited large-scale evidence on the determinants of stunting among children from infancy to primary school age. This cross-sectional study, part of South East Asian Nutrition Surveys II (SEANUTS II), aimed to determine sociodemographic and environmental risk factors for stunting among 2989 children aged 0.5–12 years. Methods: Children were recruited from four regions in Peninsular Malaysia (Central, East Coast, 2022–2030Northern, Southern). Standing height or recumbent length was measured, and stunting was classified based on WHO criteria (height-for-age Z-score below −2 standard deviations). Parents reported information on socioeconomic status, sanitation facilities, and hygiene practices. Multivariate binary logistic regression was used to determine the determinants of stunting. Results: Stunting prevalence was 8.9%, with infants (aOR = 2.92, 95%CI:1.14–7.52) and young children (aOR = 2.92, 95%CI:1.80–4.76) having higher odds than school-aged children. Key biological predictors included low birth weight (aOR = 2.41; 95%CI:1.40–4.13) and maternal height <150 cm (aOR = 2.24; 95%CI:1.36–3.70). Chinese (aOR = 0.56; 95%CI:0.35–0.88) and Indian children (aOR = 0.16; 95%CI:0.05–0.52) had a lower risk of stunting compared to Malays. Conclusions: This study highlights the ongoing challenge of childhood stunting in Malaysia, with age, birth weight, ethnicity, and maternal height identified as key determinants. These findings call for early identification of at-risk households and targeted support, especially through education and financial aid to foster healthy child growth. Full article

The Palearctic region is characterised by high endemism in the west and east, and a low endemism centre. The endemic lineages occurring at the two ends are largely distinct, and eastern endemics are typically associated with humid climates and forests, representing the start of a continuum from temperate to tropical forest groups and leading to Indo-Malay endemics. In contrast, western Palearctic endemics are typically associated with arid or seasonally dry (Mediterranean) climates and vegetation. Those lineages occurring in the central Palearctic are typically of western origin. Here, we use phylogenetic age (older than 34 million years (My)) to define a list of tetrapod and vascular plant lineages endemic to the western and central Palearctic, map their distributions at the ecoregion scale, and combine these maps to illustrate and understand lineage richness and endemism patterns. Sixty-three ancient lineages were recovered, approximately half of them reptiles, with several herbaceous and shrubby angiosperms, amphibians, and rodents, and single lineages of woody conifers, insectivores, and birds. Overall, we show high lineage richness in the western Mediterranean, eastern Mediterranean, and Iran, with the highest endemism values recorded in the western Mediterranean (southern Iberian Peninsula, southern France). This paints a picture of ancient lineage survival in areas of consistently dry climate since the Eocene, but also in association with persistent water availability (amphibians in the western Mediterranean). The almost complete absence of ancient endemic bird lineages is unusual and perhaps unique among the world’s biogeographic regions. The factors accounting for these patterns include climate since the end of the Eocene, micro-habitats and micro-climates (of mountain terrain), refugia, and patchiness and isolation (of forests). Despite their aridity adaptations, some of the lineages listed here may be tested under anthropogenic climatic change, although some may extend into the eastern Palearctic. We recommend using these lineages as flagships for conservation in the study region, where their uniqueness and antiquity deserve greater recognition. Full article

The genus Musa L. is one of the most important genera worldwide due to its use in food as a source of carbohydrates. A morphological characterization was performed to evaluate the potential of 100 accessions of Musa spp. from the Amazon region of Ecuador, applying 73 qualitative and quantitative descriptors in addition to the ecogeographic characterization. The multivariate analyses identified four large groups: The first is composed of the Musa AAB Simmonds ecotype “Hartón Plantain” and the “Cuerno Clone”. The second group is composed of the Musa acuminata Colla ecotype “Orito”. The third group is composed of the Musa acuminata ecotype “Malay plantain or red plantain”; and the fourth group is composed of the Musa × paradisiaca L. AAB ecotype “Barraganete” and banana or banana materials and the Musa AAB Simmonds ecotype “Plátano Dominico”. The qualitative descriptors with the highest discriminant value were the shape of the ♂ floret bud, the appearance of the rachis, and the pigmentation of the compound tepal, and the quantitative discriminant characters were the height of the pseudostem, the length of the leaf blade, the width of the leaf blade, and the weight of the raceme. The analysis with CAPFITOGEN of these 100 accessions through the ecogeographic characterization map identified 23 categories, highlighting category 20 with a coverage of 40.35%, which mainly includes the provinces of Orellana, Sucumbíos, part of Napo, Pastaza, and Morona Santiago. This category occurs within an annual temperature range between 21.6 °C and 27 °C, an apparent density of 1.25 to 1.44 g cm⁻³, and a cation exchange capacity (CEC) of 4 to 29 Cmol kg⁻¹. The morphological characterization of 100 Musa accessions revealed significant phenotypic variability, with four distinct morphological groups identified through cluster analysis. Key differences were observed in traits such as bunch weight, fruit length, and vegetative vigor. This variability highlights the potential of certain accessions for use in genetic improvement programs. The findings contribute valuable information for the efficient conservation, selection, and utilization of the Musa germplasm in Ecuadorian agroecosystems. The results demonstrate the existence of an important genetic variability in the INIAP Musa Germplasm Bank in the Ecuadorian Amazon region. Full article

Background/Objectives: Mango, which is known as the “King of Tropical Fruits”, is an evergreen plant belonging to the Anacardiaceae family. It belongs to the genus Mangifera, which comprises 69 species of plants found in tropical and subtropical regions, including India, Indonesia, the Malay Peninsula, Thailand, and South China. However, research on the structural information of complete chloroplast genomes of Mangifera is limited. Methods: The rapid advancement of high-throughput sequencing technology enables the acquisition of the entire chloroplast (cp) genome sequence, providing a molecular foundation for phylogenetic research. This work sequenced the chloroplast genomes of six Mangifera samples, performed a comparative analysis of the cp genomes, and investigated the evolutionary relationships within the Mangifera genus. Results: All six Mangifera samples showed a single circular molecule with a quadripartite structure, ranging from 157,604 bp to 158,889 bp in length. The number of RNA editing sites ranged from 60 to 61, with ndhB exhibiting the highest number of RNA editing sites across all species. Seven genes—namely, atpB, cemA, clpP, ndhD, petB, petD, and ycf15—exhibited a Ka/Ks value > 1, suggesting they may be under positive selection. Phylogenetic analysis revealed that Mangifera siamensis showed a close relationship between Mangifera indica and Mangifera sylvatica. Conclusions: Our comprehensive analysis of the whole cp genomes of the five Mangifera species offers significant insights regarding their phylogenetic reconstruction. Moreover, it elucidates the evolutionary processes of the cp genome within the Mangifera genus. Full article

Giant clams are ecologically important coral reef animals, with many species facing imminent local extinction. While many regions have undertaken recent assessments of their biodiversity assets, persistent gaps remain even in otherwise well-surveyed areas. This study sought to understand the geographic distribution of smaller-bodied and morphologically similar giant clams, specifically Tridacna maxima and T. noae, in the eastern Indian Ocean. Due to the difficulties in reliably identifying these species using morphological characters, we confirmed species identity and investigated intraspecific variation using sequence data from the mitochondrial cytochrome C oxidase subunit I gene (COI). Seventy whole animal vouchers were newly sampled from a 1500 km span of remote northwestern Australian coastline over a decade, as part of an ongoing coral reef survey expedition of the Western Australian Museum and partners. Tridacna maxima had a limited distribution and was only genotyped from offshore oceanic reefs in the Rowley Shoals and Cocos Keeling Islands. In contrast, T. noae was well established beyond Ningaloo Reef, and was abundant at inshore sites throughout the Pilbara and Kimberley, and even offshore to Ashmore Reef. Phylogeographically, T. maxima did not group with conspecifics from the Western Pacific Ocean, including the east coast of Australia, but instead clustered with individuals from Malaysia, China, Taiwan, and Indonesia; T. noae exhibited a similar pattern. The affinity of Western Australian individuals with representatives from the Indo-Malay region and not eastern Australia will be an important consideration for these commercially important species. Novel haplotypes in both tested species occur in Western Australia. Continued sampling of eastern and central Indian Ocean giant clams, especially to continue to document the range of T. noae, is encouraged to understand connectivity in this basin. Together, these findings contribute to an improved baseline for conservation initiatives of these iconic coral reef animals in Western Australia. Full article

This article explores the maritime connections relating to Buddhism and diplomacy between polities in Sumatra, the Malay Peninsula, the Indian subcontinent, and China from the beginning of the 11th century up to the 12th century CE. It focuses on new epigraphic evidence from Muara Jambi in the form of two inscribed strips of copper mentioning the Cūḍāmaṇivarmavihāra, a monastery funded by the king of Śrīvijaya in Nagapattinam (South India), and the Bālādityavihāra, probably located in Nālandā (Northeastern India). These new findings are compared to archaeological and textual materials from elsewhere in the Buddhist world that cast light on the web of transregional connections between Nusantara, China, and India in the early centuries of the second millennium. Full article

Background: The COVID-19 pandemic has been associated with rising obesity rates. Autistic children have a higher risk of obesity than neurotypical children. Our study aims to describe the changes in overweight/obesity rates in autistic children during the pandemic, and to identify contributing factors. Methods: This is a retrospective case record review of patients with a clinical diagnosis of autism, who were seen at a developmental-behavioral pediatrics clinic in a tertiary academic hospital, between 1 January 2019 and 24 October 2021. We compared the average monthly rates of overweight/obese status pre- and during the pandemic. We collected data on the patients’ and parents’ demographics, duration of screen time per day, degree of difficulties related to autism symptoms and cognition. We analyzed factors associated with being overweight/obese during the pandemic. Results: 1330 patient visits were included. The mean age was 45.4 months; 78% were male; 52% were Chinese. The average monthly rate of overweight/obese status increased by 1.8% during the pandemic (17.9% pre-pandemic; 19.7% during pandemic). Factors associated with being overweight/obese during the pandemic included: Malay ethnicity (OR 2.321, p < 0.01), developmental delay (OR 2.80, p < 0.01), and lower parental education level (father OR 1.73, p = 0.01; mother OR 1.63, p = 0.03). On multivariate analysis, only Malay ethnicity (OR 2.95, p = 0.01) was significant. Conclusions: Our study demonstrates a rising overweight/obesity rate amongst children with autism spectrum disorder during the pandemic. It also identified higher-risk patient profiles (Malay race, developmental delay, lower parental education). We hope this will facilitate the implementation of preventative health measures specifically supporting the high-risk children. Full article

Labisia pumila var. alata (LP) is an herbaceous shrub commonly used by women to promote health and vitality, alleviate postmenopausal symptoms, and enhance libido. Research indicates that LP possesses significant oestrogenic and antiproliferative properties towards breast cancer; however, the specific mechanisms involved remain unclear. We investigate the oestrogenic effects of LP in inducing apoptosis in human breast adenocarcinoma (MCF-7) cells and the mechanisms underlying this process. Docking analysis reveals that the phytoestrogens in LP can bind to oestrogen receptors (ER), specifically ERα and ERβ. MTT assays demonstrate that LP has a dose- and time-dependent antiproliferative effect on MCF-7 cells. Furthermore, the antiproliferative activity of LP on MCF-7 cells is inhibited by Fulvestrant, indicating that its effects are mediated through oestrogen receptors. Flow cytometry analysis shows that the antiproliferative effect of LP results from the induction of apoptosis in MCF-7 cells. The activation of caspase 3, along with caspase 8 and caspase 9, suggests that LP triggers apoptosis through both intrinsic and extrinsic pathways. The findings regarding the aqueous extract of LP and its impact on the proliferative activity of MCF-7 cells may have significant therapeutic and preventive implications for future drug development, particularly in the context of breast cancer. Full article

Background/Objectives: Work-related musculoskeletal disorders (WMSDs) have been found to be the leading factor in disabilities and absenteeism among workers. Despite their growing numbers, WMSD prevalence and risk factors among food delivery riders remain underexplored. Given the high WMSD prevalence among motorcyclists and the rising road traffic accidents (RTAs) among delivery riders in Malaysia, a cross-sectional study was conducted to identify the determinants of WMSDs among this neglected group. Methods: A self-reported questionnaire consisting of sociodemographic factors, work-related factors, and a validated Malay-translated Standardised Nordic Musculoskeletal Questionnaire (M-SNMQ) was used to evaluate the WMSD symptoms and related factors among 191 food delivery riders in Eastern Peninsular Malaysia. An ergonomic risk assessment using the Rapid Entire Body Assessment (REBA) method and whole-body vibration (WBV) measurement was also conducted to elicit the WMSD risk and vibration exposure among the riders. The determinants of WMSDs were analysed using multiple logistic regression using SPSS 20.4. Results: This study revealed a high prevalence of WMSDs (74.9%) among food delivery riders in Eastern Peninsular Malaysia. Furthermore, three factors were found to be significantly associated with WMSDs among riders, namely the increasing average working days (aOR = 2.00; 95% CI = 1.34, 2.98; p = 0.001), whole-body vibration (WBV) above the exposure action value (EAV) limit (aOR = 2.71; 95% CI = 1.13, 6.53; p = 0.026), and not exercising before work (aOR = 21.63; 95% CI = 7.45, 62.79; p < 0.001). Conclusions: Targeted interventions are essential to mitigate ergonomic risks and enhance rider safety. Occupational health policies should prioritise pre-work exercise and WBV exposure reduction to minimise musculoskeletal strain. Future longitudinal studies are needed to evaluate the long-term impact of these risks on riders’ health. Full article

The traditional Malay house is a significant component of the Malay cultural heritage and a key example of vernacular architecture. It is characterised by its outstanding design and the various styles across Malaysia. Traditional Malay houses experience deterioration and damage due to various threats, resulting in many houses being abandoned. A thorough structural evaluation is crucial for preserving the traditional Malay house. This research aimed to develop guidelines for the global structural evaluation of the Malay house. A case study approach was adopted in this research. Site visits, visual surveys, geometrical surveys, and dilapidation surveys were also employed. The research involved structural analysis using SAP2000. The results revealed the vulnerability of the houses to lateral forces, sliding, and differential settlement under scouring. The key structural members have adequate load-bearing capacity, which might be compromised under certain conditions, as in the case of deterioration. These results helped identify potential safety concerns and led to the development of guidelines for the global structural evaluation of Malay houses. The guidelines cover analysis inputs and modelling techniques in terms of material, geometry, joints, and foundations. They address load criteria and the impacts of flooding and scouring on the structural behaviour of the traditional Malay house. The guidelines, finally, recommend that structural checks be considered. This research contributes to traditional Malay house preservation by providing an evidence-based approach to designing preservation measures. Full article

Background: Men’s health behaviors influence health outcomes but remain understudied in high-risk occupational groups. This study examines determinants of health behaviors among public safety personnel using Andersen’s Behavioral Model. Methods: A cross-sectional study was conducted among 257 male public safety personnel in Kelantan, Malaysia. Participants were selected through simple random sampling. Data were collected using proforma and the validated Malay Health Behavior Inventory-Short Form (MHBI-SF). Multiple logistic regression identified factors associated with poor health behaviors. Results: Key risk factors for poor health behaviors included reliance on parental influence (AOR: 5.54; 95% CI: 1.74–17.64) and restricted healthcare access during leisure time (AOR: 4.70; 95% CI: 1.43–15.49). Protective factors included peer influence (AOR: 0.19; 95% CI: 0.05–0.71) and transportation support (AOR: 0.22; 95% CI: 0.06–0.79). Conclusions: Addressing barriers to healthcare access and enhancing social support are critical to promoting healthy behaviors among men in high-risk occupations. Targeted interventions can reduce health disparities and improve outcomes. Full article

Background/Objectives: The Child Oral Health Impact Profile—Short Form 19 (COHIP-SF 19) is widely used to measure the oral health-related quality of life (OHRQoL) of children and adolescents. The current study aimed to validate the Malay language version of the COHIP-SF19 (ML COHIP-SF 19) and to assess its psychometric properties among Malaysian children/adolescents. Methods: Children aged from 9 to 16 years attending the Faculty of Dentistry, Universiti Teknologi MARA (UiTM) participated in this study. The original English version of the COHIP-SF 19 was translated using forward- and back-translation. The psychometric properties of the final version were tested for reliability and validity using Cronbach’s alpha, a non-parametric Spearman’s correlation test, and confirmatory factor analysis (CFA). Results: A total of 252 children aged from 9 to 16 years (mean age = 11.33 ± 1.87 years) self-completed the ML-COHIP-SF 19. The total scores of the ML COHIP-SF 19 ranged from 20 to 75 (mean = 55.67 ± 10.45) with an internal consistency (α) of 0.81. Convergent validity showed a fair correlation between self-perceived oral health rating and total ML COHIP-SF 19 scores, as well as the socio-emotional well-being subscale scores (rs = 0.38–0.42, p < 0.01). Conclusions: The ML COHIP-SF 19 demonstrated reliable psychometric properties and acceptable four-factor model fits, indicating that it is a valid tool to measure the OHRQoL of Malaysian children aged from 9 to 16 years. Full article

The psychometric properties of the Malay Suicidal Behaviors Questionnaire-Revised (SBQ-R) need to be tested as it is increasingly utilized, and there is a lack of a brief, validated scale to examine suicidal behaviors in Malaysia. A total of 713 and 715 undergraduates answered the English and Malay SBQ-R, respectively. Exploratory factor analysis derived a one-factor solution, with a total explained variance of 58.0% accounted for by the four items. Confirmatory factor analyses supported the one-factor model for the Malay SBQ-R, with acceptable fit indices (χ²/df = 0.451, comparative and Tucker–Lewis fit indices = ≥1.000, standardized root mean square residual = 0.014, root mean square error of approximation = 0.000, and 90% CI [0.000, 0.083]). Measurement invariance was achieved when comparing the SBQ-R between the English and Malay versions, indicating that both versions are similar in Malaysian undergraduates. Convergent validity was established through a strong correlation between the Malay SBQ-R and the Malay Yatt Suicide Attitude Scale (r = 0.74; p < 0.001). Good internal consistency was achieved for both the English (α = 0.83; ω = 0.85) and Malay (α = 0.81; ω = 0.84) versions. The Malay SBQ-R has adequate validity and reliability for use in Malaysian undergraduates. Full article

An integrative taxonomic study recognized a new species of the Cyrtodactylus pulchellus group from Kanchanadit District, Surat Thani Province, Southern Thailand. Phylogenetic analyses of the mitochondrial ND2 gene revealed a new species, Cyrtodactylus kanchanaditsp. nov., that is closely related to C. lekaguli. The new species differs from its sister lineage by bearing mean genetic divergence of 4.53% and significant differences in both mensural and morphometric characters (p < 0.05). Cyrtodactylus kanchanaditsp. nov. can be differentiated from the remaining members of the C. pulchellus species group because of its genetic divergence of ≥7.99% and a combination of differences in body size: 11–15 supralabial and 10–12 infralabial scales, 29–35 paravertebral tubercles, 18–23 longitudinal rows of dorsal tubercles, 32–37 rows of ventral scales, 20–23 subdigital lamellae on the fourth toe, 32–37 femoroprecloacal pores in adult males, and four or five dark body bands; weak tuberculation on body; absence of tubercles in the ventral body fold, absence of precloacal pores in females, and absence of scattered pattern of white tubercles on dorsum; presence of precloacal groove depression; and maculated white caudal bands. Cyrtodactylus kanchanaditsp. nov. is currently known from its type locality. Full article