Search Results (102)

The fraction of absorbed photosynthetically active radiation (FAPAR) stands as a pivotal parameter within the Earth system, quantifying the energy exchange between vegetation and solar radiation. Accordingly, there is an urgent need for comprehensive validation studies to accurately quantify uncertainties and improve the reliability of FAPAR-based applications. This study validated five global FAPAR products, MOD15A2H, MYD15A2H, VNP15A2H, GEOV2, and GEOV3, over four boreal forest sites in North America. Qualitative quality flags (QQFs) and quantitative quality indicators (QQIs) of each product were analyzed. Time series high-resolution reference FAPAR maps were developed using the Harmonized Landsat and Sentinel-2 dataset. The reference FAPAR maps revealed a strong agreement with the in situ FAPAR from AmeriFlux (correlation coefficient (R) = 0.91; root mean square error (RMSE) = 0.06). The results revealed that global FAPAR products show similar uncertainties (RMSE: 0.16 ± 0.04) and moderate agreement with the reference FAPAR (R = 0.75 ± 0.10). On average, 34.47 ± 6.91% of the FAPAR data met the goal requirements of the Global Climate Observing System (GCOS), while 54.41 ± 6.89% met the threshold requirements of the GCOS. Deciduous forests perform better than evergreen forests, and the products tend to underestimate the reference data, especially for the beginning and end of growing seasons in evergreen forests. There are no obvious quality differences at different QQFs, and the relative QQI can be used to filter high-quality values. To enhance the regional applicability of global FAPAR products, further algorithm improvements and expanded validation efforts are essential. Full article

Cropland is a critical resource for safeguarding food security. Ensuring both the quantity and quality of cropland is essential for achieving zero hunger and promoting sustainable agriculture. However, whether urbanization-induced cropland loss poses a substantial threat to regional food security remains a key concern. This study examines the central region of the Yangtze River Delta (YRD) in China, integrating CLCD (China Land Cover Dataset) land use/cover data (2001–2023), MOD17A2H net primary productivity (NPP) data, and statistical records to evaluate the impacts of urban expansion on grain yield. The analysis focuses on three components: (1) grain yield loss due to cropland conversion, (2) compensatory yield from newly added cropland under the requisition–compensation policy, (3) yield increases from stable cropland driven by agricultural enhancement strategies. Using Sen’s slope analysis, the Mann–Kendall trend test, and hot/coldspot analysis, we revealed that urban expansion converted approximately 14,598 km² of cropland, leading to a grain production loss of around 3.49 million tons, primarily in the economically developed cities of Yancheng, Nantong, Suzhou, and Shanghai. Meanwhile, 8278 km² of new cropland was added through land reclamation, contributing only 1.43 million tons of grain—offsetting just 41% of the loss. In contrast, stable cropland (102,188 km²) contributed an increase of approximately 9.84 million tons, largely attributed to policy-driven productivity gains in areas such as Chuzhou, Hefei, and Ma’anshan. These findings suggest that while compensatory cropland alone is insufficient to mitigate the food security risks from urbanization, the combined strategy of “Safeguarding Grain in the Land and in Technology” can more than compensate for production losses. This study underscores the importance of optimizing land use policy, strengthening technological interventions, and promoting high-efficiency land management. It provides both theoretical insight and policy guidance for balancing urban development with regional food security and sustainable land use governance. Full article

Acrylamide (ACR), utilized as a precursor for producing polyacrylamide for water purification, has demonstrated neurotoxic properties. However, the mechanisms underlying its neurotoxicity remain inadequately understood. In this investigation, Caenorhabditis elegans were exposed to ACR at concentrations ranging from 250 to 1000 μg/mL and then their locomotor behavior, neuronal development, neurotransmitter concentrations, and gene expression profiles were assessed. Exposure to 250–1000 μg/mL ACR resulted in observable behaviors such as head swiveling and body bending, accompanied by a significant reduction in body size. Furthermore, ACR exposure caused damage to serotonergic, cholinergic, dopaminergic, and glutamatergic neuronal structures. In this context, elevated levels of serotonin, dopamine, acetylcholine, and glutamate were detected, along with notable upregulation of the expression of genes associated with neurotransmitters, including tph-1, cat-4, mod-1, mod-5, cat-1, ser-1, dat-1, dop-1, dop-3, unc-17, cho-1, eat-4, and glr-2. Moreover, ACR exposure elevated reactive oxygen species (ROS), O₂, and H₂O₂ levels while concurrently depleting glutathione (GSH), thereby compromising the antioxidant defense system. This led to a significant upsurge in the expression of genes involved in the nematode ACR detoxification pathway, specifically daf-16, skn-1, mlt-1, sod-3, gst-4, gcs-1, hsf-1, and hsp-16.2. Additionally, Spearman correlation analysis revealed a significant inverse relationship between certain neurotransmitter and antioxidant genes and locomotor activities, highlighting the role of these genes in mediating ACR-induced neurotoxicity in C. elegans. Collectively, this research enhances the understanding of the mechanisms related to ACR neurotoxicity. Full article

Background: With advances in radiotherapy technology, there are more technological options and fractionation regiments for different stages of lung cancer. Evaluation of the possibility of severe organ toxicity for the organ-at-risks (OARs) is often required when treating patients with unusual fractionations or combining two treatment plans with different fractionations. Purpose: This study aims to provide an approach that can reasonably estimate the possibility of toxicity using a biological equivalent mean organ dose (MOD)-based model from the 2 Gy per fraction era for various fractionations. Methods and Materials: The dose volume histograms (DVHs) of 272 patients treated with radiotherapy for lung cancer at a prescribed dose of 2 Gy × 30 f were used for analysis. For each patient, we calculated the biological equivalent MOD based on a dose conversion of EQD2 (equivalent to a dose of 2 Gy/fraction to the organ), the MOD based on the physical dose, and the d-value that makes the biological equivalent MOD based on a dose conversion of EQDd equal to the physical MOD. Statistical analysis was then performed to determine the relationship of the d-value to the corresponding physical MOD in percentage dose (relative MOD). Results: Equivalent MODs based on EQD2 were smaller than the physical MOD for each patient, suggesting that using EQD2 conversion would underestimate the equivalent MOD. The distribution of d-values with physical relative mean lung dose (RMLD) showed a normal peak with an average of d = 1.3 Gy, suggesting that the equivalent MLD can be estimated using EQD1.3 for various fractionations. Regression analysis showed that the d-value could be estimated as d = $\sqrt{0.93+3.0\times \mathrm{R}\mathrm{M}\mathrm{L}\mathrm{D}}$, d = $\sqrt{0.044+5.8\times \mathrm{R}\mathrm{M}\mathrm{E}\mathrm{D}}$, and d = $\sqrt{4.7\times \mathrm{R}\mathrm{M}\mathrm{H}\mathrm{D}}$ for the lung, esophagus, and heart, respectively, suggesting that the equivalent MLD, MED, and MHD can be estimated using EQDd. Conclusions: Using EQD2 conversion underestimates the equivalent MOD, and the equivalent MOD converted by EQDd with an appropriate d-value may enhance the assessment of organ toxicity. Full article

Objectives: Jasminoidin (JA) and ursodeoxycholic acid (UA) have been shown to exert synergistic effects on cerebral ischemia (CI) therapy, but the underlying mechanisms remain to be elucidated. Objective: To elucidate the synergistic mechanisms involved in the combined use of JA and UA (JU) for CI therapy using a driver-induced modular screening (DiMS) strategy. Methods: Network proximity and topology-based approaches were used to identify synergistic modules and driver genes from an anti-ischemic microarray dataset (ArrayExpress, E-TABM-662). A middle cerebral artery occlusion/reperfusion (MCAO/R) model was established in 30 Sprague Dawley rats, divided into sham, vehicle, JA (25 mg/mL), UA (7 mg/mL), and JU (JA:UA = 1:1) groups. After 90 minutes of ischemia, infarct volume and neurological deficit scores were evaluated. Western blotting was performed 24 h after administration to validate key protein changes. Results: Six, eleven, and four drug-responsive On_modules were identified for JA, UA, and JU, respectively. Three synergistic modules (Sy-modules, JU-Mod-7, 8, and 10) and 12 driver genes (e.g., NRF1, FN1, CUL3) were identified, mainly involving the PI3K-Akt and MAPK pathways and regulation of the actin cytoskeleton. JA and UA synergistically reduced infarct volume and neurological deficit score (2.5, p < 0.05) in MCAO/R rats. In vivo studies demonstrated that JU suppressed the expression of CUL3, FN1, and ITGA4, while it increased that of NRF1. Conclusions: JU acts synergistically on CI–reperfusion injury by regulating FN1, CUL3, ITGA4, and NRF1 and inducing the PI3K-Akt, MAPK, and actin cytoskeleton pathways. DiMS provides a new approach to uncover mechanisms of combination therapies. Full article

Background/Objectives: Modified messenger RNA (modRNA) is a promising gene delivery method used to upregulate genes in cardiac tissue, with applications in both clinical and preclinical settings to prevent cardiac remodeling after ischemic injury. The 5′ untranslated region (5′UTR) plays a crucial role in regulating the translation efficiency of mRNA into functional proteins. Due to the high production cost and short half-life of modRNA, it is essential to identify novel 5′UTR designs that enhance modRNA translation in the heart. Methods: Here, we present an artificial 5′UTR, termed “Top Heart 5′UTR”, designed based on ribonucleotide frequency analyses of 1000 genes highly expressed in the heart. This novel artificial 5′UTR contains a unique 20-nucleotide sequence, consisting of 11 previously uncharacterized nucleotides (CCCCCGCCCCC) and 9 well-described nucleotides from the Kozak sequence upstream of the start codon (ATG). Results: This design significantly improves modRNA translation efficiency in cardiomyocytes (CMs) and heart cells both in vitro and in vivo. Specifically, the Top Heart 5′UTR increases translation efficiency by approximately 30–60% in both mouse and human CMs compared to a standard 5′UTR control. Moreover, the artificial 5′UTR induces a 2–2.5 times higher translation of modRNA in the mouse heart 24 and 48 h post-delivery. Conclusions: Our findings may contribute to the development of a superior modRNA platform for use in preclinical and clinical studies, potentially allowing reduced dosages or increased gene expression at the same dosage level. This approach can be extended to identify optimized 5′UTRs for various cell types or organs, including applications in cancer therapies. Full article

Background: Del Nido (DN) cardioplegia is widely used in cardiac surgery for its efficacy in providing myocardial protection for up to 90 min with a single dose. However, its safety and efficacy during prolonged ischemia remain unclear. Methods: This retrospective study analyzed 471 patients who underwent cardiac surgery with CPB between January 2019 and September 2024. Patients were divided into two groups: ACC durations of 60–90 min (Group A, n = 240) and >90 min (Group B, n = 231). The perioperative characteristics, clinical outcomes, and biochemical markers were compared to evaluate the impact of prolonged ischemia. Results: Patients in Group B exhibited significantly higher postoperative troponin I and lactate levels at 4 h post-CPB, suggesting increased myocardial and metabolic stress. Lactate levels normalized within 24 h, indicating transient myocardial dysfunction. Defibrillation requirements and vasoactive inotropic score (VIS) were also significantly elevated in Group B, reflecting compromised myocardial electrical stability and hemodynamic challenges. However, the long-term outcomes such as mortality, LCOS, and MODS showed no significant differences between the groups. Conclusions: While DN cardioplegia provides sufficient myocardial protection for ACC durations within 90 min, its efficacy diminishes during prolonged ischemia, as evidenced by increased myocardial injury and hemodynamic instability. Tailored strategies, including standardized redosing protocols and enhanced perioperative management, are essential for optimizing outcomes in complex surgeries with extended ischemia times. Further prospective studies are needed to refine these protocols and assess alternative solutions for myocardial protection. Full article

El Niño/Southern Oscillation (ENSO) consistently modulates climate patterns in terrestrial ecosystems, triggering vegetation greening or browning. Although vegetation dynamics in the tropics during ENSO has been widely reported, the response of vegetation greening in the near-tropics to ENSO remains uncertain. Here, we explored vegetation greening near the Tropic of Cancer in China (TCC) during three sustained ENSO events during 2001–2018 based on long-term MODIS satellite Leaf Area Index (LAI) products (i.e., MOD15A2H). The results revealed a pronounced asymmetry in vegetation greening responses to ENSO near the TCC. Specifically, vegetation browning during strong La Niña (i.e., LAI anomalies about −0.15) is twice as high as vegetation greening during strong El Niño (i.e., LAI anomalies about +0.05). In La Niña, vegetation browning was accompanied by negative surface air temperature and precipitation anomalies, while in El Niño, vegetation greening was dominated by a positive anomaly in precipitation. This study emphasizes the distinct impact of ENSO on vegetation greening in the near-tropics, providing important insights into the response of vegetation dynamics to climate extremes under a warming world. Full article

Background/Objectives: Despite being widely promoted, protein supplementation’s overall effectiveness during demanding basketball schedules remains unclear. This study investigated whether increased protein intake can accelerate recovery of muscle function during a 6-day congested basketball microcycle consisting of three consecutive games while accounting for the impact of playing time. Methods: In a randomized, two-trial, cross-over, double-blind repeated measures design, eighteen male basketball players were assigned to a high (High PT) or a moderate (Mod PT) playing time group and participated in two trials, receiving daily either milk protein (PRO trial) or an isoenergetic amount of carbohydrates. Each trial included three consecutive games (days 1–3) and a 72 h recovery period following Game 3 (days 4–6), during which players participated in low-load practice sessions. Isometric and isokinetic peak torque of knee extensors and flexors in the dominant limb, serum creatine kinase (CK) concentration, and erythrocyte glutathione (GSH) levels were assessed prior to each game and practice session. Results: CK increased (p < 0.01) on game days in both groups but recovered earlier in Mod PT compared to High PT. Both eccentric and concentric peak torque was impaired (p < 0.01) up to 24–48 h post-G3 in a velocity-dependent manner. Eccentric peak torque of knee flexors at 60°/s declined to a greater extent in High PT compared to Mod PT (p < 0.01). Protein supplementation resulted in higher erythrocyte GSH concentration at pre-G2 (p < 0.05) and pre-G3 (p < 0.05) compared to placebo in both groups but did not affect any of the study outcomes. Conclusions: Increased protein intake during a congested basketball schedule increases erythrocyte GSH concentration but does not accelerate recovery of muscle function. Full article

In this work, we use the concept of $\mathfrak{G}$-weak graded rings and $\mathfrak{G}$-weak graded modules, which are based on grading by a set $\mathfrak{G}$ of left coset representatives for the left action of a subgroup $\mathfrak{H}$ of a finite $\mathfrak{X}$ on $\mathfrak{X}$, to define the conjugation action of the set $\mathfrak{G}$ and to generalize and prove some results from the literature. In particular, we prove that a $\mathfrak{G}$-weak graded ring R is strongly graded if and only if each $\mathfrak{G}$-weak graded R-module V is induced by an $R_{{\mathfrak{e}}_{\mathfrak{G}}}$-module. Moreover, we prove that the additive induction functor ${(-)}^R$ and the restriction functor ${(-)}_{{\mathfrak{e}}_{\mathfrak{G}}}$ form an equivalence between the categories $\mathfrak{G}-\mathit{GrR}-\mathit{Mod}$ and ${\mathit{R}}_{{\mathfrak{e}}_{\mathfrak{G}}}-\mathit{Mod}$ when R is strongly $\mathfrak{G}$-weak graded. Furthermore, some related results and illustrative examples of $\mathfrak{G}$-weak graded R-modules and their morphisms are provided. Full article

Potential disruptions in the biocompatibility of hyaluronic acid (HA) fillers can arise with mono-linked 1,4-butanediol diglycidyl ether (BDDE) or unreacted (pendant) 1,4-butanediol di-(propan-2,3-diolyl) ether. Assessing the filler’s degree of modification involves evaluating improperly cross-linked BDDE. This study analyzed commercially available HA fillers using nuclear magnetic resonance (NMR), focusing on key parameters, such as the degree of modification (MoD), the cross-linker ratio (CrR), and the degree of cross-linking. We assessed thirteen commercially available HA fillers using NMR. The samples were placed in an NMR instrument, and each sample was analyzed for 26 h, including MoD and CrR assessments. MoD ¹H ranged from 17.065% to 2.239%, MoD ¹³C ranged from 12.567% to 1.947%, and CrR ¹³C ranged from 0.394 to 0.014. Significant distinctions were observed in the CrR ¹³C values when the MoD values of the products were similar. This study underscores the importance of considering the MoD and the CrR together to ensure optimal cross-linking and minimize the risks associated with residual BDDE impurities. Utilizing NMR for HA gel characterization provides valuable insights regarding product quality control, safety assessments, and clinical performance evaluations for esthetic interventions, contributing to filler product improvements. Further studies correlating NMR findings with real-world outcomes are essential for ensuring safety and efficacy. Full article

The present study aimed to evaluate the potential hazardous effects of NO₃⁻ concentration in drinking water on health, feed intake, rumen fermentation and microbiota, and nitrogen excretion of Holstein heifers fed a high-concentrate diet for a sustainable water use. Twenty-four Holstein heifers were individually allocated and assigned to one of four treatments with increasing drinking water NO₃⁻ concentration: CTR, without NO₃⁻; LOW, with 44 mg NO₃⁻/L; MOD, with 110 mg NO₃⁻/L; and HIGH, with 220 mg NO₃⁻/L. The entire study lasted 168 days. Fortnightly water NO₃⁻ concentration and daily feed and water intake were recorded. Blood parameters, rumen pH, volatile fatty acids, NO₃⁻ and NO₂⁻ concentration, microbiota, and apparent total tract digestibility were determined at the beginning and at the end of the study. Most of the analyzed parameters were similar among treatments. Denitrifying bacteria population, estimated as nosZ gene copies, were greater in HIGH animals than in CTR animals at the end of the study. In conclusion, drinking water NO₃⁻ concentration up to 220 mg/L has no detrimental effect on health, feed intake, rumen fermentation, nor N excretion in dairy beef cattle for periods up to 168 days; moreover, denitrifying bacteria population increased, which are related with the neutralization of the greenhouse gas N₂O. Full article

In 2022, Cotan and Teşeleanu presented a variant of the RSA cryptosystem where the modulus is of the form $N=pq$, and the private and the public exponents satisfy $ed\equiv 1(\mathrm{mod}{\psi }_n\left(N\right))$ with $n\ge 2$, and ${\psi }_n\left(N\right)=\frac{\left(p^n-1\right)\left(q^n-1\right)}{(p-1)(q-1)}$. This variant of RSA was recently cryptanalyzed by Nitaj, Adenan, and Ariffin at Africacrypt 2024. In this paper, we push further the cryptanalysis of the scheme of Cotan and Teşeleanu by presenting a method to solve the equation $xH\left(y\right)+c\equiv 0(\mathrm{mod}e)$ where c is a constant that is independent of x and y. This enables us to propose more attacks on the scheme, including a partial key exposure attack, an attack when the most significant bits of one of the prime factors are known, and an attack when the least significant bits of one of the prime factors are known. Full article

The objective of this article is to investigate the possible correlations between vegetation indices and surface temperature in the Cananéia–Iguape Coastal System (CICS), in São Paulo (Brazil). Vegetation index data from MODIS orbital products were used to carry out this work. The Normalized Difference Vegetation Index (NDVI) and the Enhanced Vegetation Index (EVI) were acquired from the MODIS/Aqua sensor (MYD13Q1) and the leaf area index (LAI) from the MODIS/Terra (MOD15A2H). Surface temperature data were acquired from MODIS/Aqua (MYD11A2). The data were processed using Google Earth Engine and Google Colab. The data were collected, and spatial and temporal correlations were applied. Correlations were applied in the annual and seasonal period. The annual temporal correlation between vegetation indices and surface temperature was positive, but statistically significant for the LAI, with r = 0.43 (90% significance). In the seasonal period, positive correlations occurred in JFM for all indices (95% significance). Spatially, the results of this research indicate that the largest area showed a positive correlation between VI and LST. The hottest and rainiest periods (OND and JFM) had clearer and more significant correlations. In some regions, significant and clear correlations were observed, such as in some areas in the north, south and close to the city of Iguape. This highlights the complexity of the interactions between vegetation indices and climatic attributes, and highlights the importance of considering other environmental variables and processes when interpreting changes in vegetation. However, this research has significantly progressed the field, by establishing new correlations and demonstrating the importance of considering climate variability, for a more accurate understanding of the impacts on vegetation indices. Full article

Cerasus humilis, a small shrub of the Cerasus genus within the Rosaceae family, is native to China and renowned for its highly nutritious and medicinal fruits, robust root system, and remarkable drought resistance. This study primarily employed association transcriptome and metabolome analyses to assess changes in abscisic acid (ABA) levels and identify key regulatory genes in C. humilis subjected to varying degrees of drought stress. Notably, we observed distinct alterations in transcription factors across different drought intensities. Specifically, our transcriptome data indicated noteworthy shifts in GATA, MYB, MYC, WRKY, C2H2, and bHLH transcription factor families. Furthermore, combined transcriptomic and metabolomic investigations demonstrated significant enrichment of metabolic pathways, such as ‘Carbon metabolism’, ‘Biosynthesis of amino acids’, ‘Biosynthesis of cofactors’, ‘Phenylpropanoid biosynthesis’, ‘Starch and sucrose metabolism’, and ‘Plant hormone signal transduction’ under moderate (Mod) or severe (Sev) drought conditions. A total of 11 candidate genes involved in ABA biosynthesis and signaling pathways were identified. The down-regulated genes included secoisolariciresinol dehydrogenase-like and PYL2. Conversely, genes including FAD-dependent urate hydroxylase-like, cytochrome P450 97B2, carotenoid cleavage dioxygenase 4 (CCD4), SnRK2.2, ABI 5-like protein 5, PP2C 51, and SnRK2.3, were up-regulated under Mod or Sev drought stress. This study lays the genetic foundation for ABA biosynthesis to enhance drought tolerance and provides genetic resources for plant genetic engineering and breeding efforts. Full article