Search Results (18)

In northern China’s arid and semi-arid regions, evergreen trees demonstrate significant cold tolerance to natural low-temperature stress during winter. However, the metabolic strategies and their associated properties underlying their overwintering adaptation remain incompletely elucidated. This study aims to reveal the metabolic properties of natural low-temperature adaptation strategies in five evergreen trees through metabolomic analysis and to identify key metabolites and their dynamic variation patterns. The GC-TOF-MS platform was used to investigate seasonal differential metabolites in five evergreen trees across January, April, July, and October and further explore core differentially expressed metabolites responsive to low-temperature stress. The results demonstrated that the seasonal changes in the chlorophyll content of five evergreens exhibited distinct patterns, that significant differences were observed between Juniperus sabina L. and Picea meyeri R., Ammopiptanthus mongolicus M., Buxus sinica var. parvifolia M.Cheng, and Pinus tabuliformis C., and that no significant differences were found among the other tree species. A total of 427 metabolites were detected in the metabolome; when assessing seasonal dynamics, it was found that the types of differentially expressed metabolites in the five evergreens underwent significant changes. In spring, the differentially expressed metabolites included some carbohydrates, alcohols, organic acids, and lipids. During summer and autumn, the largest number of differentially expressed metabolites accumulated, mainly including carbohydrates, organic acids, and amino acid compounds. In winter, while Picea meyeri primarily accumulated carbohydrates, the remaining four species mainly accumulated organic acids, along with a small number of alcohols, phenylpropanoids, and polyketides. Three shared carbohydrate metabolites, L-threose, galactinol, and gluconic lactone, were commonly downregulated across all species. Additionally, coniferous trees collectively accumulated 3,6-anhydro-D-galactose, showing downregulation. The KEGG enrichment analysis of winter-accumulated metabolites revealed significant associations with the pentose phosphate pathway, amino acid metabolism, phenylpropanoid biosynthesis, the tricarboxylic acid cycle, and ascorbate–aldarate metabolism pathways. Through comparative analysis with the summer growth season, we ultimately identified the core differentially expressed metabolites of the five evergreens, providing potential metabolic markers for the breeding of cold-tolerant species. In summary, these findings provide critical metabolomic insights into how plants adapt to low temperatures, significantly enhancing our understanding of the metabolic foundations of cold tolerance in evergreen species. Full article

Juniperus sabina L., a species within the Juniperus genus, is broadly distributed across Southern Europe and Central Asia. In China, its berries, branches, and leaves are traditionally employed in Uyghur medicine to address conditions such as rheumatoid arthritis, itchy skin, tinnitus, deafness, and urinary discomfort. Notably, the plant’s primary bioactive constituents are terpenoids, lignans, and flavonoids. Contemporary pharmacological studies have revealed that J. sabina exhibits a range of bioactivities, including insecticidal, antimicrobial, antioxidant, anti-inflammatory, and antitumor effects. These diverse therapeutic properties can be attributed to its rich chemical profile, which comprises 279 distinct compounds such as terpenoids, lignans, flavonoids, coumarins, and others, isolated to date. This comprehensive review systematically organizes and summarizes the botanical characteristics, traditional uses, chemical composition, pharmacological activities, toxicity, limitations, and future prospects of J. sabina. It aims to offer a valuable scientific reference and fresh perspectives for further research, development, and application of this plant. Full article

Sudden cardiac death (SCD) is responsible for a high percentage of cardiovascular fatalities, with ventricular arrhythmias being the most common cause. Despite numerous clinically available antiarrhythmic drugs (AADs), AADs retain some undesirable arrhythmic effects, and their inappropriate use can lead to severe adverse reactions. The exploration of new therapeutic options against arrhythmias with fewer unreceptive effects is of utmost importance. The ethanolic extracts of seven Cupressaceae species, namely, Chamaecyparis obtusa, Juniperus chinensis (L.) Ant., Sabina chinensis (L.) Ant. cv. Kaizuca, Platycladus orientalis (L.) Franco, Juniperus sabina L., Fokienia hodginsii, and Juniperus chinensis ‘Pyramidalis’ were investigated for their pharmacological effects on barium chloride (BaCl₂)-induced arrhythmia using normal II lead electrocardiogram (ECG) measurements in a mouse model. According to the ECG profiles, pretreatment with C. obtusa, P. orientalis, and J. sabina extracts provoked dose-dependent protection against BaCl₂-induced arrhythmia, while pretreatment with the other four species and amiodarone did not exert cardioprotective effects. The treatment effects were confirmed using a rat model. The therapeutic effects of C. obtusa, P. orientalis, and J. sabina extracts on the M2 and M3 receptors but not the M1 receptor were mediated by the inhibition of the M2 receptor blocker (methoctramine tetrahydrochloride), M3 antagonist (4-DAMP), or M1 receptor blocker (pirenzepine dihydrochloride). This first-line evidence illustrates that certain Cupressaceae species possess active antiarrhythmic components. The first line of key findings revealed that active components of certain Cupressaceae species have cardioprotective effects, suggesting that these innovative phytochemicals have promising potential for preventing the occurrence of cardiac arrhythmia and reducing sudden cardiac death. Full article

Trees and shrubs belonging to the genus Juniperus L. are pivotal species in arid and semiarid ecosystems in the Northern Hemisphere. However, unfavourable phenomena are observed in their populations due to global warming. We aimed to investigate the soil requirements, genetic diversity and population history of Juniperus sabina L. from Europe, Georgia, and Kyrgyzstan. Genetic resources were evaluated in 16 populations using nuclear microsatellites, while past demographic events were described based on the chloroplast DNA haplotypes. Seven chemical parameters in 36 soil samples from the European range of J. sabina were compared. In the studied area, three distinct phylogenetic lineages corresponding to different varieties of J. sabina, namely var. sabina, var. balkanensis, and the Asian variety, were revealed. Unimodal mismatch distributions and significantly negative Tajima’s D and Fu’s Fs parameters indicated that the sabina and balkanensis varieties underwent a population expansion. Microsatellite variation was moderate, potentially influenced by inbreeding, clonal propagation, and limited gene flow between populations. Bayesian clustering revealed five genetic groups. Compared to var. sabina, the balkanensis variety occupies areas with significantly higher potassium content in the soil, which probably mitigates the adverse effects of drought in its localities. Full article

Juniperus sabina L. (J. sabina L.) represents a compelling forest species due to the presence of metabolites in its leaves, which possess diverse applications in the fields of cosmetics, pharmaceuticals, and medicine. However, the species presents difficulties with regard to natural regeneration. This research aimed to improve the propagation conditions of J. sabina by using cuttings to enhance both the rooting percentage and essential oil (EO) content in the leaves. To do this, sampled cuttings underwent pretreatment with four different doses of coconut juice (25%, 50%, 75%, and 100%) within four substrates or rooting media, namely perlite, mixed substrate, pumice, and perlite–cocopeat. The study was carried out over the course of all four growing seasons to examine the impact of harvesting time as well. The best results for rooting were observed with the pretreatment of coconut juice at 25% in spring and in the substrate of perlite–cocopeat (37.10% rooting). The coconut juice also significantly increased the percentage of EOs in spring (0.82% of essential oil yield). Beta-pinene was the essential oil component with the highest representation (34.7% in fall), whereas in the spring, the best season to collect cuttings, the dominant component was nerodiol. Our study can contribute to the planning of culture systems using cuttings and to the control of the production of EOs from the leaves of J. sabina. Full article

The essential oils of Juniperus are highly beneficial medicinally. The present study aimed to assess the chemodiversity and bioactivity of Juniperus formosana, Juniperus przewalskii, Juniperus convallium, Juniperus tibetica, Juniperus komarovii, and Juniperus sabina essential oils from the Qinghai-Tibet Plateau. The results revealed 92 components in six essential oils: α-pinene (2.71–17.31%), sabinene (4.91–19.83%), and sylvestrene (1.84–8.58%) were the main components. Twelve components were firstly reported in Juniperus oils, indicating that the geographical location and climatic conditions of the Qinghai-Tibet Plateau produced the unique characteristics of Juniperus essential oils. The chemodiversity of Juniperus essential oils varied greatly, with J. sabina having the most recognized components (64) and the highest chemodiversity (Shannon–Wiener index of 3.07, Simpson’s diversity index of 0.91, and Pielou evenness of 0.74). According to the chemodiversity of essential oils, the six plants were decided into the α-pinene chemotype (J. formosana), hedycaryol chemotype (J. przewalskii, J. komarovii, J. convallium, J. tibetica), and sabinene chemotype (J. sabina). PCA, HCA and OPLS-DA showed that J. formosana and J. sabina were distantly related to other plants, which provides a chemical basis for the classification of Juniperus plants. Furthermore, bioactivity tests exhibited certain antioxidant and antibacterial effects in six Juniperus oils. And the bioactivities of J. convallium, J. tibetica, and J. komarovvii were measured for the first time, broadening the range of applications of Juniperus. Correlation analysis of components and bioactivities showed that δ-amorphene, β-udesmol, α-muurolol, and 2-nonanone performed well in the determination of antioxidant activity, and α-pinene, camphene, β-myrcene, as well as (E)-thujone, had strong inhibitory effects on pathogenic bacteria, providing a theoretical basis for further research on these components. Full article

Juniper (Juniperus L., Cupressaceae Bartlett) trees are of high commercial value, as their essential oils are widely applied in the food and cosmetic industries due to their bioactivities. The genus Juniperus comprises eight species in Greece, and in the current work, we report the chemical analyses of their volatiles (GC-MS) obtained from the leaves and cones of all indigenous species found in the country, as well as their antimicrobial properties. The studied species were J. oxycedrus L., J. excelsa M. Bieb., J. foetidissima Willd., J. communis L., J. macrocarpa Sibth. & Sm., J. turbinata Guss., J. sabina L. and J. drupacea Labill., and a total of 164 constituents were identified. Monoterpenes, followed by sesquiterpenes, appeared as the dominant compounds in all investigated species. Most of the studied essential oils belonged to the α-pinene chemotype, with amounts of α-cedrol, sabinene, limonene and myrcene among the abundant metabolites, except for J. sabina, which belonged to the sabinene chemotype. Through antimicrobial tests, it was observed that the essential oils of most of the cones showed better activity compared with the respective leaves. The essential oils of the cones of J. foetidissima, J. communis and J. turbinata showed the strongest activity against the tested microorganisms. Additionally, in these three species, the content of thujone, which is a toxic metabolite found in essential oils of many Juniperus species, was considerably low. Taking into consideration the chemical profile, safety and antimicrobial activity, these three Greek Juniperus species seemed to provide the most promising essential oils for further exploitation in the food and cosmetics industries. Full article

Allopolyploidy is considered as a principal driver that shaped angiosperms’ evolution in terms of diversification and speciation. Despite the unexpected high frequency of polyploidy that was recently discovered in the coniferous genus Juniperus, little is known about the origin of these polyploid taxa. Here, we conducted the first study devoted to deciphering the origin of the only hexaploid taxon in Juniperus along with four of its closely related tetraploid taxa using AFLP markers with four primers combinations. Phylogenetic analysis revealed that the 10 studied species belong to 2 major clusters. J. foetidissima appeared to be more related to J. thurifera, J. sabina, and J. chinensis. The Bayesian clustering analysis showing a slight variation in genetic admixture between the studied populations of J. foetidissima, suggesting an allopolyploid origin of this species involving J. thurifera and J. sabina lineages, although a purely autopolyploidy origin of both J. thurifera and J. foetidissima cannot be ruled out. The admixed genetic pattern revealed for J. seravschanica showed that the tetraploid cytotypes of this species originated from allopolyploidy, whereas no clear evidence of hybridization in the origin of the tetraploid J. thurifera and J. chinensis was detected. This study provides first insights into the polyploidy origin of the Sabina section and highlights the potential implication of allopolyploidy in the evolution of the genus Juniperus. Further analyses are needed for a more in-depth understanding of the evolutionary scenarios that produced the observed genetic patterns. Full article

The polar fractions of the Juniperus species are rich in bioflavonoid contents. Phytochemical study of the polar fraction of Juniperus sabina aerial parts resulted in the isolation of cupressuflavone (CPF) as the major component in addition to another two bioflavonoids, amentoflavone and robustaflavone. Biflavonoids have various biological activities, such as antioxidant, anti-inflammatory, antibacterial, antiviral, hypoglycemic, neuroprotective, and antipsychotic effects. Previous studies have shown that the metabolism and elimination of biflavonoids in rats are fast, and their oral bioavailability is very low. One of the methods to improve the bioavailability of drugs is to alter the route of administration. Recently, nose-to-brain drug delivery has emerged as a reliable method to bypass the blood–brain barrier and treat neurological disorders. To find the most effective CPF formulation for reaching the brain, three different CPF formulations (A, B and C) were prepared as self-emulsifying drug delivery systems (SEDDS). The formulations were administered via the intranasal (IN) route and their effect on the spontaneous motor activity in addition to motor coordination and balance of rats was observed using the activity cage and rotarod, respectively. Moreover, pharmacokinetic investigation was used to determine the blood concentrations of the best formulation after 12 h. of the IN dose. The results showed that formulations B and C, but not A, decreased the locomotor activity and balance of rats. Formula C at IN dose of 5 mg/kg expressed the strongest effect on the tested animals. Full article

Juniperus sabina L. var. balkanensis R.P. Adams & Tashev is a recently described endemic variety from the Balkan Peninsula. Its strong sprouting ability and fast vegetative propagation, on one hand, and fragmented distribution, on the other, can lead to lower genetic diversity in local populations and to the differentiation of populations. As there has been no detailed investigation of this variety, we studied Balkan natural populations using phytochemical and molecular markers. Leaf essential oils (EOs) were chosen based on their proven usability in the population studies of Juniperus taxa, while ISSRs (Inter Simple Sequence Repeats) have been used due to their high resolution. In addition, since this variety is best described using molecular markers, the chloroplast trnS-trnG region was amplified from individuals from different populations having different chemotypes. Based on the essential oil profile, three chemotypes could be identified with a difference in their distribution. The analysis of molecular variance showed moderate differentiation of populations and regions, attesting to the start of the separation of three regions in the Balkans: west, east and south. The bioclimatic and environmental parameters and sex of the individual did not influence the EO profile, although some of the compounds present in low-to-medium concentrations showed strong correlation with several bioclimatic parameters. Full article

Juniperus sabina is an interesting species for forest restoration and ornamental purposes. The seeds of this plant have several types of dormancies; therefore, seed propagation is difficult and time consuming. The production of cuttings can be an alternative way to produce plants more quickly. The main objective of this experiment was to determine the best propagation conditions (indole butyric acid dose, substrate, and season) for this species using stem cuttings. Rooting performance of the cuttings was evaluated based on the rooting percentage (%), root biomass, and specific root length (SRL). In addition, we examined the internal composition (auxin and peroxidase content) in treated stem cuttings. Cuttings were pretreated with five doses of indole butyric acid (IBA; 0 (control), 1000, 2000, 4000, and 8000 ppm) and were rooted in four substrates (perlite, perlite-cocopeat, pumice, and mixed substrate) during the four seasons (winter, spring, summer, and autumn). The best treatments, with more than 60% rooting, were applied in spring, and IBA at 1000 ppm in perlite–cocopeat substrate obtained 62% rooting. The highest rooting percentage correlated with the highest root biomass production and the lowest SRL. IBA pretreatment decreased the concentration of peroxidase in spring (coinciding with maximum rooting), representing an indicator of rooting performance. Based on these results, we recommend a new protocol for Juniperus sabina production: (i) prepare cuttings in spring, (ii) treat cutting bases with 1000 ppm IBA, and (iii) plant cuttings in a substrate of perlite–cocopeat (1:1). Full article

Juniperus sabina L. (J. sabina) has been an important plant in traditional medicine since ancient times. Its needles are rich in podophyllotoxin, a precursor compound to anti-tumor drugs. However, no systematic research has been done on J. sabina as a source of podophyllotoxins or their biological action. Hence, extracts of podophyllotoxin and deoxypodophyllotoxin were the main optimization targets using the Box–Behnken design (BBD) and response surface methodology (RSM). The total phenol content and antioxidant activity of J. sabina needle extract were also optimized. Under the optimal process conditions (ratio of material to liquid (RLM) 1:40, 90% methanol, and ultrasonic time 7 min), the podophyllotoxin extraction rate was 7.51 mg/g DW, the highest level reported for Juniperus spp. distributed in China. To evaluate its biological potential, the neuroprotective acetyl- and butyrylcholinease (AChE and BChE) inhibitory abilities were tested. The needle extract exhibited significant anti-butyrylcholinesterase activity (520.15 mg GALE/g extract), which correlated well with the high levels of podophyllotoxin and deoxypodophyllotoxin. This study shows the potential medicinal value of J. sabina needles. Full article

Juniperus L. is the second-largest genus of conifers, having the widest distribution of all conifer genera. Its phytogeographic history is, however, obscure due to its very poor fossil record. We described a wood of Juniperus sp. from the lower Maeotian sediments of the Popov Kamen section, Taman Peninsula, South Russia, in order to clarify its taxonomic position shedding light on the phytogeographic history of the genus. This fossil wood was well-preserved by mummification, which allowed for it to be studied by the same methods as used for the anatomical examination of modern woods. The wood from the Popov Kamen section shows the greatest similarity to the extant Mediterranean species J. excelsa, belonging to the section Sabina. This is the first reliable macrofossil evidence of the sect. Sabina from Eurasia convincingly dated to the Miocene. The age of the mummified wood from the Popov Kamen section is consistent with molecular dating of diversification of the lineage comprising juniper species of the sect. Sabina from Europe, Asia and eastern Africa. The wood of Juniperus sp. has not been buried in situ, as it was found in the relatively deep-water marine sediments. The available coeval pollen series and macrofossils of Cupressaceae from the surrounding regions suggest that this wood was likely transferred by sea current from the northwestern side of the Black Sea, which was a part of the Eastern Paratethys. Full article

Juniperus sabina L., a shrub distributed in patches in arid and semi-arid areas of the northern hemisphere, plays an important role in preventing land desertification and maintaining ecosystems. However, few studies have reported genetic diversity and genetic structure of widely distributed populations of J. sabina in northwest China. Here, we evaluated the genetic diversity and genetic structure and predicted the isolation barriers among 11 populations based on 20 simple sequence repeats (SSRs). A total of 134 alleles were generated and the average number of alleles per locus was 6.70. The Shannon diversity index ranged from 0.659 to 0.951, with an average of 0.825. Population structure analysis revealed that the populations were assigned into two genetic groups. The analysis of molecular variance (AMOVA) indicated that 88% of genetic variation existed within populations. Moderate population differentiation was occurred with F_ST value of 0.090. Finally, we concluded that geographic isolation is the main factor affecting the genetic structure of J. sabina populations. The results of this study provide a foundation for the strategies for J. sabina genetic conservation and management. Full article

Little is known about the immunomodulatory activity of essential oils isolated from Juniperus species. Thus, we isolated essential oils from the cones and leaves of eight juniper species found in Montana and in Kazakhstan, including J. horizontalis, J. scopolorum, J. communis, J. seravschanica, J. sabina, J. pseudosabina, J. pseudosabina subsp. turkestanica, and J. sibirica. We report here the chemical composition and innate immunomodulatory activity of these essential oils. Compositional analysis of the 16 samples of Juniper essential oils revealed similarities and differences between our analyses and those previously reported for essential oils from this species. Our studies represent the first analysis of essential oils isolated from the cones of four of these Juniper species. Several essential oil samples contained high levels of cedrol, which was fairly unique to three Juniper species from Kazakhstan. We found that these essential oils and pure (+)-cedrol induced intracellular Ca²⁺ mobilization in human neutrophils. Furthermore, pretreatment of human neutrophils and N-formyl peptide receptor 1 and 2 (FPR1 and FPR2) transfected HL60 cells with these essential oils or (+)-cedrol inhibited agonist-induced Ca²⁺ mobilization, suggesting these responses were desensitized by this pretreatment. In support of this conclusion, pretreatment with essential oils from J. seravschanica cones (containing 16.8% cedrol) or pure (+)-cedrol inhibited human neutrophil chemotaxis to N-formyl peptide. Finally, reverse pharmacophore mapping predicted several potential kinase targets for cedrol. Thus, our studies have identified cedrol as a novel neutrophil agonist that can desensitize cells to subsequent stimulation by N-formyl peptide. Full article