Search Results (17)

Background/Objectives: Since their approval in early 2020, mRNA vaccines have gained significant attention since the COVID-19 pandemic as a potential therapeutic approach to tackle several infectious diseases. This article aims to review the current state of mRNA vaccine technology and its use against other diseases. Methods: To obtain accurate and reliable data, we carefully searched the clinicaltrial.gov and individual companies’ websites for current ongoing clinical trials reports. Also, we accessed different NCBI databases for recent articles or reports of clinical trials, innovative design of mRNA vaccines, and reviews. Results: Significant progress has been made in the design and improvement of mRNA vaccine technology. Currently, there are hundreds of ongoing clinical trials on mRNA vaccines against different cancer types, infectious diseases, and genetic and rare diseases, which showcase the advancement in this technology and their potential therapeutic advantages over traditional vaccine platforms. Finally, we predict what could be a potential future direction in designing more effective mRNA vaccines, particularly against cancer. Conclusions: The results of many of the ongoing clinical trials have shown significant positive outcomes, with many of the trials already at Phase III. Despite this outlook, however, some have been terminated or withdrawn for several reasons, some of which are not made available. This means that despite the advancement, there is a need for more research and critical evaluation of each innovation to better understand their immunological benefits and long-term effects. Full article

Background: Stunting remains a significant global health issue, particularly in low- and middle-income countries (LMICs). Globally, around 22% of children under five are affected, with high rates persisting in South and Southeast Asia. This review examines government-led programs in high-performing LMICs (Nepal, Bangladesh, and Vietnam) to identify key strategies and opportunities for effective intervention. Methods: A literature search was conducted on PubMed using keywords and Medical Subject Heading terms, including “stunting”, “child undernutrition”, “malnutrition” and the names of the three specified countries. Articles were evaluated for relevance based on their focus on stunting prevalence, risk factors, and interventions in these countries, without restrictions on publication date or language. Results: Stunting prevalence among children under five has significantly declined in Nepal, Bangladesh, and Vietnam over the past two decades, reflecting the impact of sustained nutrition and health interventions. Nepal reduced stunting from 55.8% in 2000 to 26.7% in 2022, Bangladesh from 54.7% to 26.4%, and Vietnam from 41.5% to 19.3%. Successful strategies included multisectoral approaches integrating nutrition-specific and nutrition-sensitive interventions, enhanced policy frameworks, and strong governance. Despite progress, challenges remain, such as high wasting prevalence in Nepal and disparities among marginalized communities in Vietnam, emphasizing the need for targeted, context-specific interventions. Conclusions: Effective stunting reduction requires multisectoral strategies addressing underlying, intermediate, and immediate determinants. Insights from Nepal, Bangladesh, and Vietnam highlight the importance of sustained government commitment, robust policies, and coordinated interventions. Adapting these successful strategies to local contexts can support stunting prevention and management, promoting healthier and more resilient communities. Full article

This study presents analytical models for simulating the thermal properties of linear triatomic systems, using the modified Rosen–Morse oscillator and harmonic oscillator potential to represent vibrational modes. The models employ existing partition functions to derive the thermodynamic functions for the symmetric, asymmetric, and 2-fold degenerate bending modes. These thermodynamic functions are applied to gaseous triatomic molecules such as BO₂, HCN, N₃, and Si₂N. The results demonstrate high accuracy, with mean percentage absolute deviations (MPAD) of less than 0.17% for molar entropy and Gibbs free energy. For enthalpy and heat capacity, MPAD values are below 2% compared to National Institute of Standards and Technology (NIST) data. The findings are in strong agreement with the existing literature on gaseous triatomic molecules, confirming the reliability of the proposed models. Full article

Background: Women and girls account for more than 50% of the global HIV population. In Nigeria, the proportion of women living with HIV on long-term antiretroviral therapy (ART) has been on the rise. Despite this, little research exists on their experiences regarding antiretroviral therapy use, especially for women living with HIV (WLHIV) in Plateau State, Nigeria. This study investigates the barriers and facilitators influencing antiretroviral therapy use among women living with HIV. Methods: This study employed a qualitative research design, using focus groups, and included women (female sex workers, pregnant and non-pregnant women living with HIV) and the male partners of serodiscordant couples. Eligibility criteria were being 18 years of age or older, on antiretroviral therapy for more than one year/on pre-exposure prophylaxis (PrEP) for more than one month, and speaking English, Hausa, or both. Data coding utilized both inductive and deductive approaches, and standard content analysis was applied to develop emerging themes. Results: Of the 106 participants, 88 were women living with HIV, and 18 were men in serodiscordant couples. The first facilitator shared by the participants was feeling healthier and stronger due to the antiretroviral therapy, which was also expressed by the male participants on PrEP as feeling good while taking the drug. Additional facilitators shared by the participants included weight gain and having a more positive outlook on life. Participants also disproportionately described barriers to using antiretroviral therapy, including experiences with emotional challenges, physical discomfort, and side effects of ART. Such barriers were linked to feelings of past regret, frustration, and disappointment. Conclusion: This study underscores the significance of maintaining a positive perspective on ART use, demonstrated by the connection between a positive outlook and weight gain, and highlights the hurdles that Plateau State’s women living with HIV face in adhering to antiretroviral therapy. Policymakers and healthcare providers can utilize these findings to formulate targeted strategies aimed at minimizing identified barriers and enhancing antiretroviral therapy utilization among this population via peer- support groups, economic empowerment, and psychosocial support. Full article

Household air pollution was responsible for an estimated 3.2 million deaths per year in 2020, including over 237,000 deaths of children under the age of 5. A large number of these death cases was particularly recorded in developing countries where many people rely heavily on biomass for energy. Burning biomass emits carbon monoxide and other pollutants resulting in indoor air pollution, exacerbations of asthma, hospitalizations for heart attacks and respiratory illness, birth defects, neurological diseases, and even mortality, which are all brought on by indoor air pollution. Because women and children typically do most of the cooking, they are most affected by indoor air pollution. In this research, an active sampling technique was adopted in estimating the amount of three major criteria gaseous pollutants (CO, H₂S, and SO₂) in the air in rural household kitchens within the Jos metropolis. The Attair 5X gas detector was used. The power button was pressed and the equipment was allowed to initialize for few minutes while the readings were taken downwind in-situ at a distance of 1 m, 2 m, 3 m, 4 m, and 5 m respectively from the emission source at the expiration of one (1) minute for each distance to check the impact of emissions on the environment and people in such areas. The results obtained shows that CO, H₂S, and SO₂ were higher from firewood emission sources when compared with charcoal emission sources from the 14 different rural kitchens in the Bauchi ring road, Jos, Plateau State, Nigeria. Hence, this study serves as a ready reference for environmentalists to make target decisions on air pollution reduction. Full article

Lumpy Skin disease (LSD) is an economically important disease in cattle caused by the LSD virus (LSDV) of the genus Capripoxvirus, while pseudocowpox (PCP) is a widely distributed zoonotic cattle disease caused by the PCP virus (PCPV) of the genus Parapoxvirus. Though both viral pox infections are reportedly present in Nigeria, similarities in their clinical presentation and limited access to laboratories often lead to misdiagnosis in the field. This study investigated suspected LSD outbreaks in organized and transhumance cattle herds in Nigeria in 2020. A total of 42 scab/skin biopsy samples were collected from 16 outbreaks of suspected LSD in five northern States of Nigeria. The samples were analyzed using a high-resolution multiplex melting (HRM) assay to differentiate poxviruses belonging to Orthopoxvirus, Capripoxvirus, and Parapoxvirus genera. LSDV was characterized using four gene segments, namely the RNA polymerase 30 kDa subunit (RPO30), G-protein-coupled receptor (GPCR), the extracellular enveloped virus (EEV) glycoprotein and CaPV homolog of the variola virus B22R. Likewise, the partial B2L gene of PCPV was also analyzed. Nineteen samples (45.2%) were positive according to the HRM assay for LSDV, and five (11.9%) were co-infected with LSDV and PCPV. The multiple sequence alignments of the GPCR, EEV, and B22R showed 100% similarity among the Nigerian LSDV samples, unlike the RPO30 phylogeny, which showed two clusters. Some of the Nigerian LSDVs clustered within LSDV SG II were with commonly circulating LSDV field isolates in Africa, the Middle East, and Europe, while the remaining Nigerian LSDVs produced a unique sub-group. The B2L sequences of Nigerian PCPVs were 100% identical and clustered within the PCPV group containing cattle/Reindeer isolates, close to PCPVs from Zambia and Botswana. The results show the diversity of Nigerian LSDV strains. This paper also reports the first documented co-infection of LSDV and PCPV in Nigeria. Full article

Soil loss caused by erosion has destroyed landscapes, as well as depositing sterile material on fertile lands and rivers, clogged waterways and accelerated flash floods, declined the populations of fish and other species, and diminish soil fertility. In some places, erosion has also destroyed buildings, caused mudflow, create new landforms, displaced people, and slowed down the economy of the affected community by destroying roads and homes. Erosion is aggravated by climate change and anthropogenic factors such as deforestation, overgrazing, inappropriate methods of tillage, and unsustainable agricultural practices. In this study, remote sensing (RS) and geographic information (GIS) data and tools were used to model erosion and estimate soil loss in the catchment area of Koromi-Federe in Jos East, Plateau State Nigeria which is our study area. Soil loss estimation was performed using the revised universal soil loss equation (RUSLE) model and was computed by substituting the corresponding values of each factor inherent in the equation (rainfall erosivity, soil erodibility, slope steepness and slope length, cover management, and conservation practices) using RS and GIS tools. Soil data was obtained from the study area and analyzed in the laboratory, rainfall data, land cover, digital elevation model (DEM), as well as the management practice of the study area were the parameters computed in spatial analyst tool using map algebra based on RUSLE. The soil loss generated was classified into four classes and the results revealed 95.27% of the catchment with a tolerable loss of less than 10 t/h⁻¹/y⁻¹. At 3.6%, a low or minimal loss of 10–20 t/h⁻¹/y⁻¹, at 1.03% there exists a moderate loss of 20–50 t/h⁻¹/y⁻¹, while there was and critical or high loss of >50 t/h⁻¹/y⁻¹ at 0.12% of the catchment. The result showed that critical soil loss in the catchment area is exacerbated by the influence of the slope length and steepness, and the amount of rainfall received. This poses great concern with annual rainfall projected to increase up to 12% in West Africa. However, our sensitivity analysis revealed that it can be reduced with the effect of vegetated cover and management practices. This is an important finding as it can guide sustainability practices to control erosion and the loss of valuable lands in the region, especially now under climate change. Full article

The rise of bat-associated zoonotic viruses necessitates a close monitoring of their natural hosts. Since the detection of severe acute respiratory syndrome coronavirus (SARS-CoV), it is evident that bats are vital reservoirs of coronaviruses (CoVs). In this study, we investigated the presence of CoVs in multiple bat species in Nigeria to identify viruses in bats at high-risk human contact interfaces. Four hundred and nine bats comprising four bat species close to human habitats were individually sampled from five states in Nigeria between 2019 and 2021. Coronavirus detection was done using broadly reactive consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene of CoVs. Coronavirus RNA was detected in 39 samples (9.5%, CI 95%: [7.0, 12.8]), of which 29 were successfully sequenced. The identified CoVs in Nigerian bats were from the unclassified African alphacoronavirus lineage and betacoronavirus lineage D (Nobecovirus), with one sample from Hipposideros ruber coinfected with alphacoronavirus and betacoronavirus. Different bat species roosting in similar or other places had CoVs from the same genetic lineage. The phylogenetic and evolutionary dynamics data indicated a high CoV diversity in Nigeria, while host switching may have contributed to CoV evolution. Robust sentinel surveillance is recommended to enhance our knowledge of emerging and re-emerging coronaviruses. Full article

Reliable crop type maps from satellite data are an essential prerequisite for quantifying crop growth, health, and yields. However, such maps do not exist for most parts of Africa, where smallholder farming is the dominant system. Prevalent cloud cover, small farm sizes, and mixed cropping systems pose substantial challenges when creating crop type maps for sub-Saharan Africa. In this study, we provide a mapping scheme based on freely available Sentinel-2A/B (S2) time series and very high-resolution SkySat data to map the main crops—maize and potato—and intercropping systems including these two crops on the Jos Plateau, Nigeria. We analyzed the spectral-temporal behavior of mixed crop classes to improve our understanding of inter-class spectral mixing. Building on the Framework for Operational Radiometric Correction for Environmental monitoring (FORCE), we preprocessed S2 time series and derived spectral-temporal metrics from S2 spectral bands for the main temporal cropping windows. These STMs were used as input features in a hierarchical random forest classification. Our results provide the first wall-to-wall crop type map for this key agricultural region of Nigeria. Our cropland identification had an overall accuracy of 84%, while the crop type map achieved an average accuracy of 72% for the five relevant crop classes. Our crop type map shows distinctive regional variations in the distribution of crop types. Maize is the dominant crop, followed by mixed cropping systems, including maize–cereals and potato–maize cropping; potato was found to be the least prevalent class. Plot analyses based on a sample of 1166 fields revealed largely homogeneous mapping patterns, demonstrating the effectiveness of our classification system also for intercropped classes, which are temporally and spatially highly heterogeneous. Moreover, we found that small field sizes were dominant in all crop types, regardless of whether or not intercropping was used. Maize–legume and maize exhibited the largest plots, with an area of up to 3 ha and slightly more than 10 ha, respectively; potato was mainly cultivated on fields smaller than 0.5 ha and only a few plots were larger than 1 ha. Besides providing the first spatially explicit map of cropping practices in the core production area of the Jos Plateau, Nigeria, the study also offers guidance for the creation of crop type maps for smallholder-dominated systems with intercropping. Critical temporal windows for crop type differentiation will enable the creation of mapping approaches in support of future smart agricultural practices for aspects such as food security, early warning systems, policies, and extension services. Full article

Larks constitute an avian family of exceptional cryptic diversity and striking examples of convergent evolution. Therefore, traditional morphology-based taxonomy has recurrently failed to reflect evolutionary relationships. While taxonomy ideally should integrate morphology, vocalizations, behaviour, ecology, and genetics, this can be challenging for groups that span several continents including areas that are difficult to access. Here, we combine morphometrics and mitochondrial DNA to evaluate the taxonomy of Calandrella larks, with particular focus on the African C. cinerea and the Asian C. acutirostris complexes. We describe a new range-restricted West African taxon, Calandrella cinerea rufipecta ssp. nov. (type locality: Jos, Plateau State, Nigeria), with an isolated relic population 3000 km from its closest relative in the Rift Valley. We performed molecular species delimitation, employing coalescence-based multi-rate Poisson Tree Processes (mPTP) on cytochrome b sequences across 52 currently recognized lark species, including multiple taxa currently treated as subspecies. Three species-level splits were inferred within the genus Calandrella and another 13 across other genera, primarily among fragmented sub-Saharan taxa and taxa distributed from Northwest Africa to Arabia or East Africa. Previously unknown divergences date back as far as to the Miocene, indicating the presence of currently unrecognized species. However, we stress that taxonomic decisions should not be based on single datasets, such as mitochondrial DNA, although analyses of mitochondrial DNA can be a good indicator of taxa in need of further integrative taxonomic assessment. Full article

This study evaluated the effects of cooking for different hydrothermal-treatment durations (10, 20, 30 and 40 min) on the proximate composition, amino acid profile, fatty acid composition and organoleptic parameters of filets of African catfish Clarias gariepinus (Burchell, 1822). Filets of the fish were prepared from market size African catfish with similar breeding history. Parameters of the processed filet under the different hydrothermal durations were also compared against a raw–unprocessed control group except during organoleptic analysis. The results obtained revealed a significant increase in protein, fat and ash content until the 30th minute of hydrothermal processing (p ≤ 0.05). Beyond this processing time, protein and fat significantly reduced while ash remains unaffected. The same trend was observed for most essential/non-essential amino acids isolated as well as the prominent saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids. In all, the raw control group consistently recorded the least values of nutritional components. The perception of assessors was, however, found to be similar (p ≥ 0.05) in terms of organoleptic parameters regardless of the duration of the processing time of the filets. It was concluded that cooking the African catfish filet using the hydrothermal method should not be extended beyond 30 min. Full article

Nearly 290,000 tons of electronic waste was generated in Nigeria during the year 2017, which is likely to increase further due to high population growth rates, accelerated urbanization, high demand for electronic products, as well as disposal at their end-of-life. At the same time, e-waste is associated with negative human health impacts, as well as environmental pollution. Therefore, environmental awareness on this waste stream is crucial in its management and possible minimization. In this paper, we report on a survey which investigated awareness levels and knowledge amongst households in the Jos metropolis, Plateau State (Nigeria). We interviewed 228 respondents by means of close-ended questionnaires. The results indicated that cell phones (93%) and television sets (82%) were mentioned by most respondents. The main reasons for acquiring these electronic devices entailed the replacement of damaged ones (49.6%), frequent product upgrades (37.7%), as well as theft (35.55%), amongst others. The most predominant method of disposing e-waste included illegal dumping in open spaces along with other household wastes (25%), storing it at home indefinitely (27.6%), and selling it to others for possible reuse (17.5%). Although the handling and storage of this waste is currently inappropriate, most respondents (84.2%) were willing to participate in its management provided they are given appropriate knowledge (89.9%) on its safe disposal and recycling. Lastly, we found no significant correlation between existing awareness levels on e-waste and willingness to participate in its management based on the socio-demographical profile of respondents. Thus, we recommend educational interventions on sound e-waste management in the Jos metropolis, along with a systematic analysis of how policy interventions such as the extended producer responsibility schemes can be designed for effective e-waste management and recycling amongst all stakeholders. Full article

This study investigated the physical and combustion properties of briquettes produced from agricultural wastes (groundnut shells and corn cobs), wood residues (Anogeissus leiocarpus), and mixture of the particles at 15%, 20%, and 25% starch levels (binder). A 6 × 3 factorial experiments in a Completely Randomized Design (CRD) was adopted for the study. The briquettes produced were analyzed for density, volatile matter, ash content, fixed carbon, and specific heat of combustion. The result revealed that the density ranged from 0.44 g/cm³ to 0.53 g/cm³, while briquettes produced from groundnut shells had the highest (0.53 g/cm³) significant mean density. Mean volatile matter and ash content of the briquettes ranged from 24.35% to 34.95% and 3.37% to 4.91%. A. leiocarpus and corn cobs particles had the lowest and highest ash content, respectively. The briquette fixed carbon and specific heat of combustion ranged from 61.68% to 68.97% and 7362 kcal/kg to 8222 kcal/kg, respectively. Briquette produced from A. leiocarpus particles had the highest specific heat of combustion. In general, briquettes produced from A. leiocarpus particles and mixture of groundnut shell and A. leiocarpus particles at 25% starch level had better quality in terms of density and combustion properties and thus are suitable as an environmentally friendly alternative energy source. Full article

Schistosomiasis is a debilitating disease affecting over 200 million people, with the highest burden of morbidity and mortality in African countries. Despite its huge impact on the health and socio-economic burden of the society, it remains a neglected tropical disease, with limited attention from governments and stakeholders in healthcare. One of the critical areas that is hugely under-developed is the development of accurate diagnostics for both intestinal and urogenital schistosomiasis. Diagnosis of schistosomiasis is important for the detection and treatment of disease in endemic and non-endemic settings. A conclusive detection method is also an indispensable part of treatment, both in the clinic and during mass drug administration (MDA), for the monitoring efficacy of treatment. Here, we review the available diagnostic methods and discuss the challenges encountered in diagnosis in resource limited settings. We also present the available diagnostics and cost implications for deployment in resource limited settings. Lastly, we emphasize the need for more funding directed towards the development of affordable diagnostic tools that is affordable for endemic countries as we work towards the elimination of the disease. Full article

The aetiological agent of rabies is a member of the Lyssavirus genus (Rhabdoviridae family, order Mononegavirales). The disease (rabies) is endemic in many parts of Asia and Africa and still remains an important public and veterinary health threat. In Nigeria, there is a dearth of information on the natural infection and/or exposure of bat species to lyssaviruses. Therefore, this study was undertaken to assess the prevalence of rabies virus (RABV) neutralizing antibodies in sera obtained from bats from the central Plateau and North-East Bauchi States in Nigeria. Two hundred serum samples were collected from Nigerian fruit bats from six different locations and tested for anti-RABV antibodies using a commercial blocking ELISA. Of the 200 bat serum samples collected, one batch consisting of 111 samples did not meet the validation criteria and hence was not included in the final analysis. Of the remaining 89, only three (3.4%) contained anti-lyssavirus antibodies, demonstrating a low prevalence of lyssavirus antibodies in the study population. In order to further understand the exposure of bat species to phylogroup II lyssaviruses (Lagos bat virus and Mokola virus), the same panel of samples will be tested for neutralizing antibodies to phylogroup II members, viruses that do not cross-neutralize with members of phylogroup I. Full article