Search Results (166,351)

Quinoa (Chenopodium quinoa Willd.) is a nutrient-dense pseudocereal with a favorable nutritional profile that may improve postprandial glycemic control and satiety. This randomized crossover pilot study compared the acute effects of cooked quinoa and white bread on postprandial blood glucose and subjective appetite responses in healthy young adults. Participants consumed four test meals on separate occasions: cooked quinoa or white bread providing either 25 g or 35 g available carbohydrate. Capillary blood glucose and appetite ratings were assessed over 120 min. Quinoa produced significantly lower postprandial glucose responses and incremental area under the curve (iAUC) than white bread at both carbohydrate doses (p < 0.05). Appetite responses were also improved following quinoa consumption compared to the control. Blood glucose iAUC was positively correlated with appetite net area under the curve (r = 0.57; p < 0.001). Palatability and gastrointestinal tolerance were comparable between treatments. These findings indicate that cooked quinoa elicits more favorable postprandial glycemic and satiety responses than white bread and may serve as a functional carbohydrate alternative to support metabolic health. Full article

Net primary productivity (NPP) is a core diagnostic variable of terrestrial carbon cycling, yet consistent annual NPP simulation remains challenging in alpine heterogeneous regions where topography, hydrothermal gradients, vegetation structure, and nutrient constraints interact. Remote-sensing products such as MODIS provide valuable observational constraints, whereas light-use-efficiency models such as CASA retain process transparency and scenario transfer capability. This study develops a CASA-based, MODIS-constrained framework for annual NPP simulation over the Gannan Plateau. The framework preserves a locally parameterized CASA baseline and adds a geographically weighted regression (GWR) residual-alignment layer trained on CASA–MODIS residuals during 2005–2013. The fitted correction relationship was then applied to the 2014–2020 temporal transfer period and evaluated in a 2030s SSP scenario transfer experiment. MODIS was treated as the correction target rather than ground truth, and GLASS was adopted as an independent product-level benchmark. During 2014–2020, the GWR-corrected product showed improved pooled pixel-level agreement with the MODIS-constrained target relative to parameter-localized CASA, with R² increasing from 0.438 to 0.708 and RMSE decreasing from 91.4 to 68.7 g C m⁻² yr⁻¹. Residual Moran’s I also decreased, indicating weaker residual spatial organization after correction. Product-level comparison with GLASS showed a moderate, directionally consistent improvement relative to uncorrected CASA, although this comparison was not interpreted as ground-truth validation. The 2030s scenario transfer experiment indicated that the correction layer changed the spatial expression of NPP divergence among SSP pathways. Overall, the proposed framework provides a process-model-preserving and observation-constrained approach for improving agreement between annual NPP estimates and the MODIS-constrained target in alpine heterogeneous regions, while its applicability remains subject to product uncertainty, spatial dependence, and future nonstationarity. Full article

In this study, a MIL-100(Fe)@KTBC composite was successfully fabricated via an in situ hydrothermal method using KOH-activated tomato-biochar-derived carbon (KTBC) as the support, and was applied for the efficient adsorptive removal of xylenol orange (XO) from water. Characterization by SEM, XRD, FTIR, XPS, and BET confirmed that MIL-100(Fe) was successfully loaded onto the KTBC surface, and the resulting composite exhibited a well-developed porous structure, abundant functional groups, and good thermal stability. Adsorption experiments showed that MIL-100(Fe)_0.5@KTBC delivered the optimal performance, with a maximum adsorption capacity of 246.12 mg/g; high removal efficiency was achieved at pH 4.0 and an adsorbent dosage of 0.4 g/L. The adsorption process followed pseudo-second-order kinetics and the Langmuir isotherm model, indicating spontaneous, endothermic, monolayer adsorption dominated by chemisorption. The composite also demonstrated strong resistance to interfering ions and favorable reusability. This work provides a scientific basis for the development of efficient and stable biochar-based MOF composites for the treatment of printing and dyeing wastewater Full article

Porcine epidemic diarrhea virus (PEDV) is a major enteric coronavirus that causes severe economic losses to the global swine industry. Current vaccines suffer from weak immunogenicity, insufficient mucosal immunity, and a short duration of protection. Nanoparticle delivery systems have emerged as a promising strategy for next-generation vaccine development. In the present study, we constructed an S1-Ferritin nanoparticle vaccine using the SpyTag-SpyCatcher modular conjugation system. The morphology, particle size, and uniformity of the nanoparticle vaccine were systematically characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). After two immunizations, the S-Ferritin nanoparticle vaccine elicited potent humoral and cellular immune responses in both mice and piglets. In piglets, at 2 weeks post booster vaccination, PEDV-specific serum IgG endpoint titers peaked at 1:2560, virus-neutralizing antibody titers reached 1:256 against the JS-2/2015 strain, and serum IFN-γ levels reached 274 pg/mL. All these immunological indicators were significantly higher than those observed in the PEDV-inactivated whole-virus vaccine group. Furthermore, challenge tests showed that the S1-Ferritin nanoparticle vaccine provided complete protection against PEDV infection and significantly reduced the severity of diarrhea and intestinal damage in piglets after challenge. These findings suggest that the S1-Ferritin nanoparticle vaccine constitutes a promising candidate against PEDV infection, though our study is only a preliminary step and subsequent field trials in pigs are still required. Full article

Foot-worn electromagnetic harvesters are driven by irregular rigid-body motion, while their response is limited by mechanical stroke, coil geometry, mounting direction, and the electrical interface. This paper presents a reproducible numerical design study of a finite-stroke magnetic quasi-zero-stiffness (QZS) moving-magnet harvester. Two public three-axis foot-IMU records are processed with stated gyroscope-bias estimation, six-axis attitude estimation, gravity removal, residual-offset correction, filtering, and angular-acceleration calculation. Three explicit axes are used in the design screen, and the selected candidate is then evaluated over a 62-direction spherical grid. Rigid-body angular-acceleration and centripetal terms are included for specified sensor-to-harvester offsets. Two normalized magnetic force laws are compared. The electrical model uses position-dependent flux linkage, explicit series connection and polarity of coil sections, winding-derived resistance, and a position-dependent electromagnetic reaction force. A fixed-seed random screen evaluates 720 geometry-constrained candidates. The highest-ranked nominal candidate is a 150 mm external foot-worn module with a 40.6 g moving mass, a 30 mm hard half-stroke, 1649 turns in two series sections, and a 25.27 mm coil outer diameter. Across 72 design-screen cases formed from 12 five-second windows, three mounting axes, and two magnetic laws, this candidate remained hard-stroke- and design-stroke-safe. Its conditional ideal load-side power had a 10th percentile of 1.38 mW and a median of 2.03 mW. In the 62-direction check, all 1488 cases remained hard-stroke-safe; two opposite directions each produced one design-stroke exceedance, with a maximum displacement of 24.15 mm. Re-ranking all 30 Stage-2 candidates under coupling and magnetic-stiffness changes retained the long geometry family, although a 30% coupling reduction changed the highest-ranked candidate from 600 to 632. Soft-stop sensitivity, equation-level consistency, and multi-case Runge–Kutta convergence are also reported. The results support finite-stroke design screening, but they do not constitute prototype, finite-element, or delivered-power validation. Full article

Phenolic compounds are valued for their antioxidant activity, but their measured response after heating can vary with the surrounding matrix. In this study, dried Cynanchum thesioides (Freyn) K. Schum powder (CTP) was added to a cookie matrix at 0%, 1%, 3%, and 5% (w/w) to examine phenolic indices, antioxidant activity, and physicochemical changes after baking. CTP affected moisture content, spread ratio, hardness, color, pH, and soluble solids in the baked matrix, whereas density was unchanged. Antioxidant activity increased with increasing CTP level. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities increased from 8.04% to 46.33% and from 17.02% to 36.43%, respectively, while ferric reducing antioxidant power (FRAP) increased from 6.17 to 23.64 mM FeSO₄ equivalents/g. Total polyphenol content (TPC) increased from 0.66 to 1.46 mg gallic acid equivalents/g, and total flavonoid content (TFC) increased from 0.20 to 0.85 mg catechin equivalents/g. These results show that increasing CTP raised the extractable phenolic indices, radical scavenging activity, and reducing capacity measured in the baked samples. The findings establish a formulation-dependent antioxidant response in the final baked products and provide a basis for future compound-specific studies of phenolic retention during processing. Full article

Rapid growth in connected devices, artificial intelligence applications, and the Internet of Things (IoT) is driving demand for ultra-high data rates, low latency, and energy-efficient communication infrastructure. Wireless optical communication (WOC), including free-space optical (FSO), is recognized as a disruptive technology for 6G and future-generation networks. However, atmospheric visibility critically affects WOC/FSO link availability, capacity, and reliability. This study proposes a machine learning (ML)-based low-visibility classification model that integrates Meteorological Aerodrome Reports (METARs) with microwave-link received-signal (Rx) features. Visibility below 6000 m is predicted at the 1 h, 3 h, and 6 h horizons using 18 months of data from Suvarnabhumi Airport (VTBS) and Don Mueang Airport (VTBD) in Bangkok, Thailand. Four ML algorithms, namely logistic regression, random forest, extreme gradient boosting, and light gradient boosting machine (LGBM), are evaluated against persistence and Terminal Aerodrome Forecast (TAF) baselines. In a 100-round block-bootstrap evaluation, LGBM with METAR-Rx achieved the highest mean F1 scores at the 1 h and 3 h horizons, outperforming TAF by 28 and 20 percentage points at the 1 h horizon for VTBS and VTBD, respectively. SHAP and ablation analyses suggested that current visibility is the dominant predictor, while Rx features provide complementary information and improve F1 performance by approximately 1–4 percentage points. Seasonal analysis shows stronger cool-season performance, while rainy-season prediction remains challenging. Adding visibility-trend features further improves performance, with the best combined model achieving 1 h F1 scores of 0.7253 for VTBS and 0.6495 for VTBD. These findings indicate that integrating the METAR-Rx feature set can support short-term low-visibility classification. Full article

Arid inland saline lakes are key components of basin ecosystems. As the largest saline lake in Xinjiang and a critical ecological barrier in northwest China, Ebinur Lake’s area dynamics are vital to regional sustainable development. This study integrates Landsat imagery (1992–2024) with meteorological and socio-economic data to investigate optimal water extraction methods, spatio-temporal lake area variations, and driving mechanisms. Multiple methods were employed, including water index comparison, Mann–Kendall test, Pearson correlation, and ridge regression. Results show that: (1) the Normalized Difference Water Index (NDWI) maintains high, stable classification accuracy across years and months, making it suitable for long-term monitoring; (2) from 1992 to 2024, lake area demonstrates a significant fluctuating downward trend without abrupt change points, indicating continuous degradation. During the growing season (April–October), it first decreases and then increases, with larger early-season areas, minima in August and September, coinciding with peak agricultural irrigation demand; (3) regarding driving mechanisms, socio-economic factors dominate (approximately 70%), while meteorological factors play a weakly regulatory role (about 30%). Population growth and increased water consumption are the primary drivers, with obvious seasonal differences. Meteorological changes, socio-economic development, and ecological measures jointly influence lake area. Although extreme events (e.g., anomalous precipitation) induce short-term fluctuations, they do not alter the long-term degradation trend dominated by human activities. This study provides methodological support for long-term monitoring of arid saline lakes and scientific evidence for ecological conservation and water resource management in the Ebinur Lake Basin. Full article

Background/Objectives: Adolescent pregnancy is associated with an increased risk of adverse neonatal outcomes, including low birth weight, preterm birth, and impaired neonatal adaptation. While maternal age is a well-established determinant, the independent role of parity in this population remains uncertain. This study aimed to assess the association between parity and neonatal outcomes in adolescent mothers. Methods: We conducted a retrospective cohort study including 751 singleton live-birth deliveries to adolescent mothers aged <18 years at a Romanian secondary-care hospital. The unit of analysis was the delivery episode recorded in the institutional birth registry. Mothers were classified according to parity at the time of the index delivery as primiparous or multiparous. Neonatal outcomes included low birth weight (<2500 g), preterm birth (<37 weeks), Apgar score < 7 at 5 min, and a composite adverse neonatal outcome. Multivariable logistic regression models were used to evaluate the association between parity and neonatal outcomes, adjusting for maternal age (<15 vs. ≥15 years), gestational age, and mode of delivery, as appropriate. Results: A total of 751 singleton live-birth delivery episodes were included; 609 (81.1%) occurred in primiparous adolescents and 142 (18.9%) in multiparous adolescents. Parity was not significantly associated with preterm birth (aOR 0.86, 95% CI 0.48–1.56, p = 0.622), Apgar score < 7 at 5 min, or the composite adverse neonatal outcome. Maternal age < 15 years and gestational age were independently associated with Apgar score < 7 at 5 min. Multiparity was associated with lower odds of low birth weight (aOR 0.40, 95% CI 0.17–0.92, p = 0.031). Conclusions: Parity was not independently associated with most neonatal outcomes in adolescent pregnancies. Multiparity was associated with lower odds of low birth weight, but this finding should be interpreted cautiously because residual confounding cannot be excluded. Full article

Progressive Familial Intrahepatic Cholestasis (PFIC) is a rare liver disorder that, although typically present in childhood, can also occur in adulthood. Early diagnosis is crucial for appropriate clinical management, prognostic assessment, and therapeutic decision-making; however, it remains challenging due to phenotypic variability and the frequent identification of genetic variants of uncertain significance (VUS). Advances in next-generation sequencing (NGS) and whole-exome sequencing (WES) have improved the detection of disease-associated variants, revealing that the same genetic variants—often in a heterozygous state—may lead to adult-onset cholestatic disease, with milder or atypical presentations, while still conferring a significant risk of progressive liver injury and related complications. To assess the diagnostic yield and clinical utility of NGS panels and WES in adults with suspected PFIC or unexplained cholestatic liver disease, focusing on variant interpretation, emerging disease-associated genes, and clinical significance of VUS. A literature review was conducted to assess molecular diagnostics in adult cryptogenic cholestasis, with a focus on studies employing targeted NGS panels and WES. Selected studies were examined for diagnostic yield, variant classification, interpretive challenges, including VUS, and integration with clinical data. Data on patient demographics, sequencing techniques, and variant interpretation strategies were extracted to evaluate the current capabilities and limitations of genomic testing. From an initial search of 211 publications, 15 studies met the inclusion criteria, comprising five large adult cohorts and ten single-patient or trio-based reports. Across 72 patients, sequencing technologies identified 75 pathogenic or likely pathogenic variants, predominantly missense mutations, demonstrating high genetic heterogeneity. The most implicated genes included ABCB4, ABCB11, ATP8B1, TJP2, and USP53, with diagnostic yields ranging from 13.2% in large heterogeneous cohorts to substantially higher rates in carefully selected individual cases. VUS were identified in up to 67% of cases, highlighting the need for multidisciplinary interpretation integrating clinical, biochemical, and genetic data. Emerging evidence also implicated ciliopathy-associated genes such as DCDC2, NPHP3, PKHD1, TULP3, and TTC21B, expanding the genetic spectrum of adult cholestasis. Clinically, presentations ranged from mild biochemical abnormalities to progressive cholestasis, with pruritus being a common feature across studies. Comprehensive genetic testing significantly enhances diagnostic accuracy, informs prognosis, and guides individualized management in adults with cryptogenic cholestasis. Emerging evidence suggests that ciliopathy-associated genes may contribute to the genetic architecture of adult cholestatic disorders, further expanding the spectrum of disease-associated genes. However, the high prevalence of VUS remains a major challenge, highlighting the need for functional studies, longitudinal follow-up, and improved variant interpretation frameworks. As genomic technologies and analytical approaches continue to evolve, genetic testing is expected to play an increasingly central role in precision hepatology. Full article

In this study, a bio-based compatibilizer, itaconic acid-grafted polypropylene (PP-g-IA; IP), was synthesized via melt grafting as a sustainable alternative to the conventional petroleum-based maleic anhydride-grafted polypropylene (PP-g-MAH; MP). Successful grafting was confirmed by Fourier-Transform Infrared (FT-IR) spectroscopy, and the efficacy of the synthesized compatibilizer was systematically evaluated by applying it to composites incorporating both unmodified lignocellulose (LC) and hydrophobically modified LC (mLC). Mechanical evaluation revealed that the PP-g-IA-compatibilized LC composite (PPLCIP) achieved the highest tensile strength of 23.5 MPa among all samples. This result is attributed to a synergistic effect: the PP-g-IA compatibilizer significantly improved the interfacial adhesion issue, thereby allowing the intrinsically higher aspect ratio of unmodified LC fibers (3.0 ± 2.1) to serve as the dominant reinforcement factor. While mLC surface modification improved composite performance over unmodified LC even without a compatibilizer, the introduction of a compatibilizer shifted fiber aspect ratio to the dominant factor, resulting in LC-based composites outperforming their mLC-based counterparts. Furthermore, PP-g-IA maintained mechanical performance comparable to that of the conventional PP-g-MAH while simultaneously achieving enhanced melt flowability. These results demonstrate that the bio-based PP-g-IA is an effective and sustainable compatibilization strategy that satisfies both mechanical performance and processability requirements. Full article

Members of the yellow gene family encode conserved Yellow proteins with diverse roles in insect pigmentation, cuticle formation, and reproduction, making them promising targets for RNA interference (RNAi)-mediated pest control strategies. However, little is known about their phylogeny and functions in the brown planthopper (Nilaparvata lugens), a devastating rice pest that serves as a vector for viral rice diseases. Here, we identified and characterized 11 yellow genes in N. lugens, which showed distinct structures and expression profiles. Phylogenetic analysis revealed that N. lugens has retained orthologs of yellow-y, -b, -d, -e, -g, -g2, -h, and -x but lacks those of yellow-c and -f, suggesting lineage-specific gene loss. Nine of the eleven NlYellow genes were localized to chromosome 8, with several positioned in close genomic proximity. The clustering of these loci suggests that tandem duplication events contributed to the expansion of the NlYellow gene family. NlYellow-b exhibited the highest transcript levels among all identified NlYellow genes, particularly in the female ovipositor, and functional characterization via RNAi revealed that it is essential for molting, wing development, and female fertility. Knockdown of NlYellow-b in fourth-instar nymphs caused lethal molting defects and wing malformation and significantly reduced offspring production. These findings highlight the dual roles of NlYellow-b in development and reproduction, advance our understanding of yellow gene function in insects and highlight NlYellow-b as a candidate for RNAi-based management of N. lugens. Full article

In this work, three biochars were synthesized from a mixture of an abundant agricultural waste (date palm fronds) and an animal biomass (chicken manure) at pyrolysis temperatures of 700 °C (B-700), 800 °C (B-800), and 900 °C (B-900), respectively. These biochars were characterized and used as electrode materials in a capacitive deionization (CDI) process to remove salts from saline water. The CDI results show that the B-700 electrode displayed the highest desalination efficiency of 10.2% with 100 ppm NaCl. Further mixing the B-700 with 10% and 20% of multi-walled carbon nanotubes (CNT) revealed an enhanced desalination performance. For instance, a biochar-20%CNT electrode achieved a salt adsorption capacity (SAC) of 11.32 mg/g at 200 ppm NaCl, which is 7.6 times higher than that of B-700 alone. The performance enhancement is attributed to carbon nanotubes acting as conductive channels between biochar particles, thereby improving electrical conductivity and electrochemical properties of the CDI electrode. Additionally, the presence of well-known hydrophilic functional groups on CNT surfaces enhances hydrophilicity, providing a highly porous surface area. The results suggest that the CDI method with biochar–CNT electrodes offers opportunities for energy-efficient, low-cost freshwater production, with significant scaling up potential for the treatment of brackish and wastewater. Full article

The emergence of multidrug-resistant (MDR) bacteria, particularly methicillin-resistant Staphylococcus aureus (MRSA), represents a major global health challenge by limiting the current therapeutic options. In this context, antimicrobial peptides (AMPs) have been widely studied for their potent antimicrobial properties. In this study, we evaluated the anti-MRSA effect of a novel AMP, LyeTx I mnΔKL, derived from a toxin of Lycosa erythrognatha. Its activity was evaluated in vitro by minimal inhibitory and bactericidal concentrations (MIC and MBC), antibiofilm effect, membrane interaction, cytotoxicity, synergistic interaction with vancomycin, and in vivo in an MRSA murine wound/abscess infection model. LyeTx I mnΔKL showed enhanced antimicrobial activity against clinical MRSA isolates compared to its prototype (LyeTx I mnΔK), with MIC₅₀ and MBC₅₀ of 2 and 8 µM and 16 and 32 µM, respectively. Furthermore, LyeTx I mnΔKL exhibited a rapid bactericidal effect and a pronounced ability to inhibit biofilm formation and disrupt mature biofilms. LyeTx I mnΔKL interacts with bacterial membranes, adopts an α-helical structure, and induces membrane disruption and leakage of intracellular material. In vivo, topical treatment with LyeTx I mnΔKL reduced MRSA burden compared with LyeTx I mnΔK and untreated controls (log₁₀ CFU/g of wound of 2.4, 4.4, and 6.5 for LyeTx I mnΔKL, LyeTx I mnΔK, and the saline group, respectively). However, increased cytotoxicity remains a significant limitation. Overall, LyeTx I mnΔKL is a promising anti-MRSA candidate for topical use with potent antibiofilm and in vivo activity. Full article

This work provides a comprehensive review of the development and current research status of micro-arc oxidation (MAO) coatings on 6061 aluminum alloy. It presents research findings on the enhancement of wear resistance, corrosion resistance, and other functional properties (e.g., hydrophobicity, thermal control performance, and electrical insulation) using three main strategies: insitu growth via electrolyte composition modification or electrical parameter adjustment, incorporation of nanoparticles into the electrolyte, and hybrid processes combining MAO with other surface treatment techniques (e.g., pre-/post-treatments). The performance of coatings obtained under different strategies is summarized, and a prospective outlook on future development trends in this field is offered. Full article