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Keywords = Eragrostis tef

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18 pages, 2657 KB  
Article
Mechanical and Chemical Weed Control in Teff in the Mediterranean Area
by Vittorio Monni, Euro Pannacci and Francesco Tei
Agronomy 2026, 16(6), 618; https://doi.org/10.3390/agronomy16060618 - 14 Mar 2026
Viewed by 645
Abstract
Teff [Eragrostis tef (Zucc.) Trotter] is attracting growing interest in Europe due to its nutritional qualities, gluten-free nature, and drought tolerance; however, its cultivation is hindered by its limited yield potential and the lack of authorised herbicides. This study evaluated chemical and [...] Read more.
Teff [Eragrostis tef (Zucc.) Trotter] is attracting growing interest in Europe due to its nutritional qualities, gluten-free nature, and drought tolerance; however, its cultivation is hindered by its limited yield potential and the lack of authorised herbicides. This study evaluated chemical and mechanical weed-control strategies using two sowing methods to identify effective and sustainable solutions under central Italian conditions. Two field trials were conducted in 2023 and 2024 using a randomised block design. Post-emergence herbicides and mechanical control (split-hoe and finger-weeder) were assessed for weed suppression, crop selectivity, biomass production, and grain yield, comparing broadcast and wide-row sowing. The results showed that chemical control was the most effective option. The florasulam + fluroxypyr + pyroxsulam mixture achieved a nearly complete weed suppression with only mild and temporary phytotoxicity. Mechanical control provided a moderate and variable efficacy. The sowing pattern significantly influenced the crop performance: broadcast sowing reduced the weed competition and resulted in higher yields, whereas wide-row sowing led to a higher weed density and lower productivity. Despite the varying levels of infestation between years, teff maintained a remarkable competitive ability, with untreated plots often achieving acceptable yields. Integrating selective herbicides with appropriate sowing practices supports the development of efficient and sustainable weed-management strategies for teff cultivation. Full article
(This article belongs to the Section Weed Science and Weed Management)
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19 pages, 1265 KB  
Article
White Teff Flour Ethanolic Extract: Phytochemical Profile, Antioxidant and Anti-Inflammatory Activity
by Ioana Ferențiu, Tiberia Ioana Pop, Alina Elena Pârvu, Iulia Ioana Morar, Dinu Bolunduț, Marcel Pârvu, Florica Ranga, Ciprian Ovidiu Dalai, Mădălina Țicolea and Raluca Maria Pop
Molecules 2026, 31(4), 644; https://doi.org/10.3390/molecules31040644 - 13 Feb 2026
Cited by 1 | Viewed by 703
Abstract
Teff (Eragrostis tef) is a gluten-free cereal increasingly promoted as a functional food, yet the bioactive profile and mechanistic evidence of some varieties remain limited. This study characterized an ethanolic extract of white teff flour and evaluated its antioxidant and anti-inflammatory [...] Read more.
Teff (Eragrostis tef) is a gluten-free cereal increasingly promoted as a functional food, yet the bioactive profile and mechanistic evidence of some varieties remain limited. This study characterized an ethanolic extract of white teff flour and evaluated its antioxidant and anti-inflammatory potential in vitro and in a rat model of acute inflammation. White teff flour was extracted by cold repercolation (1 g/mL; 70% ethanol). Total polyphenols and flavonoids were quantified spectrophotometrically, and phenolics were profiled by HPLC-DAD-ESI-MS. Antioxidant activity was assessed using DPPH, FRAP, H2O2 scavenging, and NO scavenging assays. In vivo, acute inflammation was induced with intramuscular turpentine in Wistar rats, testing teff extract therapeutically (post-induction) and prophylactically (10-day pretreatment), with diclofenac and Trolox as comparators. Serum oxidative stress biomarkers (TOS, TAC, OSI, AOPP, MDA, NOx, 3-NT, thiols) and inflammatory mediators (NFκB-p65, IL-1β, IL-18, caspase-1, IL-10) were measured. The extract showed low total polyphenols (0.044 ± 0.002 mg GAE/g d.w.) and higher flavonoids (11.83 ± 1.10 mg QE/100 g d.w.). Eighteen phenolics were identified (total 398.30 ± 1.48 μg/mL), dominated by flavone derivatives (notably apigenin- and luteolin-glycosides), while phenolic acids accounted for ~33.21%. In vitro antioxidant capacity was robust (DPPH 286.17 ± 11.52 μg TE/g d.w.; FRAP 263.17 ± 20.09 μg TE/g d.w.; H2O2 214.12 ± 18.22 mg TE/g d.w.; NO 300.77 ± 28.71 mg QE/g d.w.). In vivo, turpentine provoked marked oxidative stress and inflammatory activation; teff, particularly at the highest concentration and in prophylaxis, reduced nitro-oxidative damage markers (AOPP, MDA, NOx, 3-NT) and lowered NFκB-p65, IL-1β, IL-18, and caspase-1, while IL-10 was not significantly altered. White teff flour ethanolic extract contains a flavone-rich phenolic profile and exerts measurable antioxidant and anti-inflammatory effects in an acute inflammation model, supporting its potential development as a nutraceutical candidate. Full article
(This article belongs to the Special Issue Chemical Constituents and Biological Activities of Natural Sources)
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20 pages, 1685 KB  
Article
Nutritional and Antioxidant Profile of Brown Eragrostis tef (Zucc.) Trotter Flour in Blends with Glycine max (L.) Merr. Flour
by Shewangzaw Addisu Mekuria, Kamil Czwartkowski and Joanna Harasym
Molecules 2026, 31(2), 365; https://doi.org/10.3390/molecules31020365 - 20 Jan 2026
Viewed by 855
Abstract
The still-growing demand for nutritious gluten-free products necessitates the development of a composite flour that addresses the nutritional deficiencies common in conventional gluten-free formulations. This study aimed to comprehensively characterize brown teff (Eragrostis tef (Zucc.) Trotter) and soybean (Glycine max (L.) [...] Read more.
The still-growing demand for nutritious gluten-free products necessitates the development of a composite flour that addresses the nutritional deficiencies common in conventional gluten-free formulations. This study aimed to comprehensively characterize brown teff (Eragrostis tef (Zucc.) Trotter) and soybean (Glycine max (L.) Merr.) composite flours at 0%, 10%, 20%, 30%, and 40% soybean inclusion levels (w/w) to establish evidence-based formulation guidelines for future products. Proximate composition, antioxidant properties (total polyphenol content—TPC, antioxidant capacity vs. 2,2-diphenyl-1-picrylhydrazyl radical—DPPH and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical—ABTS, ferric reducing antioxidant power—FRAP), particle size distribution, pasting properties, color characteristics, and molecular fingerprints (Fourier transform infrared spectroscopy—FTIR) were evaluated. A principal component analysis (PCA) was employed to identify compositional–functional relationships. Soybean inclusion significantly enhanced protein content from 9.93% (pure teff) to 23.07% (60:40 blend, dry matter), fat from 2.14% to 10.47%, and fiber from 3.43% to 6.72%. The antioxidant capacity increased proportionally with soybean content, with a 40% inclusion yielding FRAP values of 5.19 mg FeSO4/g DM and TPC of 3.44 mg GAE/g DM. However, pasting viscosity decreased notably from 12,198.00 mPa·s (pure teff) to 129.00 mPa·s (60:40 blend), indicating a reduced gel-forming capacity caused by soybean addition. PCA revealed that nutritional composition (PC1: 70.6% variance) and pasting properties (PC2: 21.0% variance) vary independently, suggesting non-additive functional behavior in blends. Brown teff–soybean blends at a 20–30% soybean inclusion optimize the balance between protein enhancement, antioxidant preservation, and the maintenance of functional properties suitable for traditional applications, providing a nutritionally superior alternative for gluten-free product development. Full article
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17 pages, 745 KB  
Article
Functional and Rheological Properties of Gluten-Free Flour Blends from Brown Eragrostis tef (Zucc.) Trotter and Glycine max (L.) Merr
by Shewangzaw Addisu Mekuria, Damian Marcinkowski and Joanna Harasym
Molecules 2025, 30(24), 4817; https://doi.org/10.3390/molecules30244817 - 18 Dec 2025
Cited by 1 | Viewed by 944
Abstract
The increasing prevalence of celiac disease and demand for nutritious gluten-free alternatives have driven interest in cereal–legume composite flours. This study examined the functional, rheological, and textural properties of gluten-free flour blends formulated from brown (red) teff (Eragrostis tef (Zucc.) Trotter) and [...] Read more.
The increasing prevalence of celiac disease and demand for nutritious gluten-free alternatives have driven interest in cereal–legume composite flours. This study examined the functional, rheological, and textural properties of gluten-free flour blends formulated from brown (red) teff (Eragrostis tef (Zucc.) Trotter) and soybean (Glycine max (L.) Merr.) at different ratios (100:0, 90:10, 80:20, 70:30, 60:40, 0:100). Absorptive characteristics, particle size distribution, pasting behaviour, viscoelastic properties through oscillatory rheology, and texture profile analysis were evaluated. Soybean flour exhibited higher water holding capacity (5.54 g/g) and water solubility index (40.18%), while teff demonstrated notable water absorption index (5.62 g/g) and swelling power (6.18 g/g). Particle size analysis revealed that coarse fractions enhanced water binding and solubility, whereas fine fractions favoured hydration and swelling. Pasting properties showed that teff achieved a peak viscosity of 12,198 mPas in water, significantly reduced to 1839 mPas with AgNO3. Pure teff exhibited the highest storage modulus (1947.98 Pa) and hardness (7.60 N), whereas the incorporation of soybeans progressively softened the texture. The complementary functional properties of teff and soybean demonstrate promising potential for developing nutritionally enhanced, protein-enriched gluten-free products, with solvent selection and blending ratios serving as critical optimization parameters for specific food applications. Full article
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32 pages, 496 KB  
Review
Sustainability of Animal Production Chains: Alternative Protein Sources as an Ecological Driver in Animal Feeding: A Review
by Massimiliano Lanza, Marco Battelli, Luigi Gallo, Francesca Soglia, Fulvia Bovera, Francesco Giunta, Riccardo Primi, Luisa Biondi, Diana Giannuzzi, Marco Zampiga, Nicola Francesco Addeo, Antonello Cannas, Pier Paolo Danieli, Bruno Ronchi and Gianni Matteo Crovetto
Animals 2025, 15(22), 3245; https://doi.org/10.3390/ani15223245 - 8 Nov 2025
Cited by 6 | Viewed by 2615
Abstract
Sustainability of animal production requires reducing reliance on soybean meal by identifying viable alternative protein sources. Within the framework of the Italian Agritech National Research Center, seven Italian research groups collaborated to evaluate unconventional feed ingredients and their effects on animal performance and [...] Read more.
Sustainability of animal production requires reducing reliance on soybean meal by identifying viable alternative protein sources. Within the framework of the Italian Agritech National Research Center, seven Italian research groups collaborated to evaluate unconventional feed ingredients and their effects on animal performance and product quality. Alternative legume seeds (peas, chickpeas, faba bean, and lupins) can partially or completely replace soybean meal without impairing productivity, while enhancing product health value and shelf-life through bioactive compounds. Microalgae (Chlorella, Spirulina) improved carotenoid content, antioxidant activity, fatty acid profile, and cholesterol levels in poultry products, with limited effects in pigs. Insects supported optimal growth in fish at 25–30% inclusion, whereas maximum recommended levels are 15% in broilers and 24% in laying hens to sustain growth, egg production, and quality. Camelina by-products are suitable for poultry diets at up to 5–10%, beyond which performance declines. Whole-plant soybean silage, tef (Eragrostis tef), and triticale–lupin intercropping represent promising protein-rich resources for ruminants, provided diets maintain balanced protein-to-energy ratios, adequate fibre characteristics, and appropriate harvest timing under drought-prone conditions. Collectively, these findings highlight the potential of diverse protein sources to improve the sustainability of livestock systems while preserving productivity and enhancing the nutritional quality of animal-derived foods. Full article
(This article belongs to the Section Animal Nutrition)
16 pages, 602 KB  
Review
Nutritional Characteristics, Health-Related Properties, and Food Application of Teff (Eragrostis tef): An Overview
by Boyiza Samson Abebe, Iuliana Aprodu, Daniela Ionela Istrati and Camelia Vizireanu
Int. J. Mol. Sci. 2025, 26(19), 9293; https://doi.org/10.3390/ijms26199293 - 23 Sep 2025
Cited by 9 | Viewed by 3627
Abstract
Teff [Eragrostis tef (Zucc.) Trotter] is a globally recognized ancient grain renowned for its attractive nutritional profile and diverse potential applications. Considering its physicochemical characteristics, nutritional value, and probable applications is essential for optimizing its benefits across various food industries. This review [...] Read more.
Teff [Eragrostis tef (Zucc.) Trotter] is a globally recognized ancient grain renowned for its attractive nutritional profile and diverse potential applications. Considering its physicochemical characteristics, nutritional value, and probable applications is essential for optimizing its benefits across various food industries. This review aims to comprehensively investigate teff, its physicochemical characteristics, diverse dietary applications, and health benefits. Teff is rich in macro- and micronutrients, making it an excellent addition to various food products. Bioactive compounds, such as flavonoids and phenolic acids, also enhance their functionality. Therefore, teff appears to be a whole grain with favorable technological characteristics and nutritional benefits for various food applications. Also, being gluten-free, teff is acceptable for individuals with celiac disease or gluten sensitivity. Also, it reduces cholesterol levels, supports heart health, stabilizes blood sugar levels, strengthens bone density and strength, and provides immune system support. In conclusion, teff shows excellent potential for developing innovative and nutritious solutions to meet the growing needs of consumers. Full article
(This article belongs to the Special Issue Role of Natural Compounds in Human Health and Disease)
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17 pages, 1222 KB  
Article
Plant-Based Burgers Made with Green Banana Biomass (GBB) Associated with Teff and Chickpea Derivatives
by Ziane da Conceição das Mercês, Natalia Maldaner Salvadori, Sabrina Melo Evangelista, Tatiana Barbieri Cochlar, Cristine da Silva Medeiros, Rafaela Giuliana Hermelino Lima, Amanda Soares Bandeira, Ana Karolina Fortunato de Souza, Alessandro de Oliveira Rios and Viviani Ruffo de Oliveira
Foods 2025, 14(10), 1782; https://doi.org/10.3390/foods14101782 - 17 May 2025
Cited by 2 | Viewed by 1812
Abstract
The objective of this study was to develop meat analog burgers using green banana biomass (GBB) combined with teff and chickpea derivatives, as well as to evaluate their chemical and technological quality, in addition to comparing them with industrialized meat and plant-based burgers. [...] Read more.
The objective of this study was to develop meat analog burgers using green banana biomass (GBB) combined with teff and chickpea derivatives, as well as to evaluate their chemical and technological quality, in addition to comparing them with industrialized meat and plant-based burgers. Four formulations (F1: 100% GBB; F2: 75% GBB; F3: 60% GBB; and F4: 50% GBB, with 25% teff and 25% chickpeas) were developed and compared to the following industrialized burgers: F5 (meat-based) and F6 (plant-based). All the samples were subjected to physical (initial/final weight, diameter, height, color, and texture) and chemical (pH, proximate composition, fiber, and caloric value) analyses. Among the treatments, F4 showed the highest initial weight (223.00 g) and final weight (201.66 g), initial diameter (12.33 cm) and final diameter (11.96 cm), and a reduction in height from 2.04 cm to 1.57 cm. In terms of firmness, F4 was significantly higher than that of the other treatments (p ≤ 0.05). Regarding its chemical composition, F4 presented a high protein content (10.25%) and energy value (285.30 kcal). The total fiber content for F1 to F4 was 3.62%, 3.74%, 3.97%, and 4.15%, respectively, while F6 (plant-based) reached 5.69%. These findings indicate that the combination of GBB with teff and chickpeas, especially in F4, was favorable for producing meat analog burgers with promising technological and nutritional properties. Full article
(This article belongs to the Section Plant Foods)
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10 pages, 485 KB  
Article
Seeding Rate Effects on Smooth Bromegrass (Bromus inermis Leyss.) Interseeded with Annual Warm-Season Grasses
by John A. Guretzky, Heidi Hillhouse and Keith R. Harmoney
Agronomy 2025, 15(4), 885; https://doi.org/10.3390/agronomy15040885 - 31 Mar 2025
Cited by 1 | Viewed by 943
Abstract
Interseeding pastures with annual warm-season grasses may increase forage accumulation and nutritive value. Our objective was to evaluate the effects of seeding rates of crabgrass [Digitaria ischaemum (Schreb.) Schreb. Ex Muhl], sorghum–sudangrass (Sorghum bicolor × S. bicolor var. sudanense), and [...] Read more.
Interseeding pastures with annual warm-season grasses may increase forage accumulation and nutritive value. Our objective was to evaluate the effects of seeding rates of crabgrass [Digitaria ischaemum (Schreb.) Schreb. Ex Muhl], sorghum–sudangrass (Sorghum bicolor × S. bicolor var. sudanense), and teff [Eragrostis tef (Zuccagni) Trotter] on the forage accumulation and nutritive value of pastures of smooth bromegrass (Bromus inermis Leyss.), an introduced perennial cool-season grass cultivated for pasture and hay production in the U.S. Western Corn Belt. In spring, before interseeding, forage accumulation averaged 4.03 and 6.39 Mg ha−1 in 2020 and 2021, respectively. In summer, after interseeding, forage accumulation averaged 3.52 Mg ha−1 in 2020 but was not affected by treatment. In 2021, forage accumulation averaged 6.22 Mg ha−1 in sorghum–sudangrass interseeded stands compared to 4.08 Mg ha−1 in non-seeded smooth bromegrass. Interseeding crabgrass and teff had limited effects on forage accumulation and nutritive value. Increasing the seeding rate of sorghum–sudangrass linearly increased yield of crude protein, total digestible nutrients, and dry matter. In the next spring, forage accumulation averaged 8.01 Mg ha−1, and the stands showed no residual effects of the one-time interseedings. Sorghum–sudangrass proved to be the optimum annual warm-season grass for interseeding. Full article
(This article belongs to the Special Issue Managing the Yield and Nutritive Value of Forage and Biomass Crops)
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21 pages, 3284 KB  
Article
Transcriptomic Profile of Tef (Eragrostis tef) in Response to Drought
by Lorena Ramirez-Gonzales, Gina Cannarozzi, Abiel Rindisbacher, Lea Jäggi, Regula Schneider, Annett Weichert, Sonia Plaza-Wüthrich, Solomon Chanyalew, Kebebew Assefa and Zerihun Tadele
Plants 2024, 13(21), 3086; https://doi.org/10.3390/plants13213086 - 2 Nov 2024
Cited by 3 | Viewed by 2558
Abstract
The threat to world food security posed by drought is ever increasing. Tef [Eragrostis tef (Zucc.) Trotter] is an allotetraploid cereal crop that is a staple food for a large population in the Horn of Africa. While the grain of tef provides [...] Read more.
The threat to world food security posed by drought is ever increasing. Tef [Eragrostis tef (Zucc.) Trotter] is an allotetraploid cereal crop that is a staple food for a large population in the Horn of Africa. While the grain of tef provides quality food for humans, its straw is the most palatable and nutritious feed for livestock. In addition, the tef plant is resilient to several biotic and abiotic stresses, especially to drought, making it an ideal candidate to study the molecular mechanisms conferring these properties. The transcriptome expression of tef leaf collected from plants grown under drought conditions was profiled using RNA-Seq and key genes were verified using RT-qPCR. This study revealed that tef exhibits a complex molecular network involving membrane receptors and transcription factors that regulate drought responses. We identified target genes related to hormones like ABA, auxin, and brassinosteroids and genes involved in antioxidant activity. The findings were compared to physiological measurements such as changes in stomatal conductance and contents of proline, chlorophyll and carotenoid. The insights gained from this work could play vital role in enhancing drought tolerance in other economically important cereals such as maize and rice. Full article
(This article belongs to the Special Issue Crop Breeding for Food and Nutrition Security)
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18 pages, 1247 KB  
Review
Unlocking the Potential of Teff for Sustainable, Gluten-Free Diets and Unravelling Its Production Challenges to Address Global Food and Nutrition Security: A Review
by Mary Adepoju, Carol Verheecke-Vaessen, Laxmi Ravikumar Pillai, Heidi Phillips and Carla Cervini
Foods 2024, 13(21), 3394; https://doi.org/10.3390/foods13213394 - 25 Oct 2024
Cited by 12 | Viewed by 6511
Abstract
Sustainable diets, as defined by the Food and Agriculture Organisation, aim to be nutritionally adequate, safe, and healthy, while optimising natural and human resources. Teff (Eragrostis tef), a gluten-free grain primarily grown in Ethiopia, has emerged as a key contender in this [...] Read more.
Sustainable diets, as defined by the Food and Agriculture Organisation, aim to be nutritionally adequate, safe, and healthy, while optimising natural and human resources. Teff (Eragrostis tef), a gluten-free grain primarily grown in Ethiopia, has emerged as a key contender in this context. Widely regarded as a “supergrain”, teff offers an outstanding nutrition profile, making it an excellent choice for people with gluten-related disorders. Rich with protein, essential amino acids, polyunsaturated fats, and fibre, and abundant in minerals like calcium and iron, teff rivals other popular grains like quinoa and durum wheat in promoting human health. Beyond its nutritional benefits, teff is a hardy crop that thrives in diverse climates, tolerating both drought and waterlogged conditions. Due to its resilience and rich nutrient content, teff holds the potential to address nine of the 17 United Nations’ Sustainable Development Goals (SDGs), including SDG 1 (no poverty), SDG 2 (zero hunger), and SDG 3 (good health and wellbeing), which are tied to improving food and nutrition security. However, teff production in Ethiopia faces significant issues. Traditional farming practices, insufficient storage infrastructure, and food safety challenges, including adulteration, hinder teff’s full potential. This review explores teff’s dual role as a nutritious, sustainable food source and outlines the key challenges in its production to conclude on what needs to be done for its adoption as a golden crop to address global food and nutrition security. Full article
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12 pages, 1058 KB  
Article
Prospect for Fine and Coarse Coal Waste Deployment for a Constructed Technosol and Eragrostis Tef Growth
by Eduardo Kercher de Oliveira, Arthur Cesa Venturella, Jéssica Weiler and Ivo André Homrich Schneider
Mining 2024, 4(4), 806-817; https://doi.org/10.3390/mining4040045 - 13 Oct 2024
Cited by 3 | Viewed by 2687
Abstract
The aim of this study was to evaluate soil properties and Eragrostis tef (teff) growth on Technosols produced from coarse and fine coal wastes from Moatize Mine, Mozambique. The experiment was performed in triplicate in 30 L containers filled with different substrate conditions, [...] Read more.
The aim of this study was to evaluate soil properties and Eragrostis tef (teff) growth on Technosols produced from coarse and fine coal wastes from Moatize Mine, Mozambique. The experiment was performed in triplicate in 30 L containers filled with different substrate conditions, composed of fine coal waste, coarse coal waste, agricultural soil, and sewage sludge as an organic matter source. The soil analyses included bulk density, available water capacity, permeability, and fertility. Plant growth was monitored for biomass production and plant tissue composition. All the substrates presented a good available water capacity and a proper drainage condition. Regarding soil fertility, there were shortages of potassium and boron in the substrates composed exclusively of coal wastes, which was reflected in the composition of the plant tissue. Even so, plant growth was statistically equivalent to the control in all conditions, except for the substrate produced exclusively with fine coal waste and sewage sludge, which presented a better performance. Technosols are an alternative for reducing the final mine waste volume, and Eragrostis tef is used as a means for land use after the mining process, with social gains, and as a tool in an ecological process for restoring coal mining sites. Full article
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11 pages, 315 KB  
Article
Production of Protein Hydrolysates Teff (Eragrostis tef) Flour with Antioxidant and Angiotensin-I-Converting Enzyme (ACE-I) Inhibitory Activity Using Pepsin and Cynara cardunculus L. Extract
by Gregorio Molina-Valero, Laura Buendía-Moreno, Cindy Bande-De León, Estefanía Bueno-Gavilá and Luis Tejada
Curr. Issues Mol. Biol. 2024, 46(10), 11303-11313; https://doi.org/10.3390/cimb46100672 - 11 Oct 2024
Cited by 3 | Viewed by 1968
Abstract
In recent years, several studies have shown the antioxidant and antihypertensive potential of bioactive peptides. Thus, bioactive peptides are likely to be a valuable substance for the development of functional foods. There are a wide variety of sources of these peptides, including several [...] Read more.
In recent years, several studies have shown the antioxidant and antihypertensive potential of bioactive peptides. Thus, bioactive peptides are likely to be a valuable substance for the development of functional foods. There are a wide variety of sources of these peptides, including several cereals. Teff is an Ethiopian-rooted cereal with an interesting nutritional profile, mainly due to its high amount of protein. In this study, teff flour was subjected to a defatting process for optimizing the protein extraction. Such extraction was performed by precipitation from its isoelectric point, a crucial step that separates the protein from other components based on their charge. The protein obtained was subjected to enzymatic hydrolysis by pepsin and Cynara cardunculus L. The antihypertensive (angiotensin-I-converting enzyme ―ACE-I― inhibitory activity) and antioxidant activity (2,2-diphenyl-1-picrylhydrazyl ―DPPH― radical scavenging activity) of the peptides were determined. According to the IC50 values, the results obtained showed that the peptides from teff flour show promising bioactivity compared to other cereals. Furthermore, the peptides from teff flour obtained from C. cardunculus L. showed higher antioxidant activity (defatted teff flour ―DTF―: 0.59 ± 0.05; protein extract ―EP― : 1.04 ± 0.11) than those obtained with pepsin (DTF: 0.87 ± 0.09; EP: 1.73 ± 0.11). However, C. cardunculus L. hydrolyzate peptides showed lower inhibitory activity of ACE-I (DTF: 0.59 ± 0.07; EP: 0.61 ± 0.05) than the pepsin hydrolyzate (DTF: 0.15 ± 0.02; EP: 0.33 ± 0.05). Full article
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16 pages, 2388 KB  
Article
Anti-Growth and Anti-Metastatic Potential of Raw and Thermally Treated Eragrostis tef Extract in Human Cancer Cells
by Jina Seo, Hwa Jin Lee and Jihyeung Ju
Nutrients 2024, 16(16), 2612; https://doi.org/10.3390/nu16162612 - 8 Aug 2024
Cited by 1 | Viewed by 1827
Abstract
Teff (Eragrostis tef), a gluten-free cereal crop cultivated originally in Northeast Africa, is increasingly utilized due to its nutritional and health benefits. The aim of the present study was to investigate the effects of ethanol extract obtained from raw and thermally [...] Read more.
Teff (Eragrostis tef), a gluten-free cereal crop cultivated originally in Northeast Africa, is increasingly utilized due to its nutritional and health benefits. The aim of the present study was to investigate the effects of ethanol extract obtained from raw and thermally treated teff, referred to as RTE and TTE, respectively, on uncontrolled growth and activated metastasis using human cancer cell lines. Both RTE and TTE contained flavones, such as orientin (luteolin 8-C-glucoside) and vitexin (apigenin 8-C-glucoside), and phenolic acids, such as protocatechuic acid and p-coumaric acid. TTE showed higher total phenol, protocatechuic acid, and p-coumaric acid contents, but lower orientin content compared to RTE. RTE and TTE significantly suppressed cell growth of H1299 human lung cancer cells, with TTE exhibiting more pronounced effects than RTE, while both extracts had only minimal effects on the growth of non-malignant human umbilical vein endothelial cells. The growth-inhibitory activities of RTE and TTE in H1299 cells were associated with apoptosis induction and cell cycle arrest at the G2/M phase. TTE produced an additional effect on inducing cell cycle arrest at the S phase in H1299 cells, potentially contributing to its stronger growth-inhibitory effects. Moreover, both RTE and TTE effectively inhibited key events in metastasis, such as invasion, migration, and adhesion, in H1299 cells under non-cytotoxic conditions, with TTE showing stronger effects. In HCT116 human colon cancer cells, a similar pattern of inhibition was demonstrated against the metastatic events, accompanied by reduced levels of matrix metalloproteinase-2 and -9. Our results indicate that teff extracts exhibit in vitro anti-growth and anti-metastatic activities, which are enhanced by thermal treatment of teff. Full article
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13 pages, 383 KB  
Article
The Effects of Harvest Maturity of Eragrostis tef ‘Moxie’ Hay and Supplemental Energy Source on Forage Utilization in Beef Heifers
by Allison V. Stevens, Cheyanne A. Myers, John B. Hall and Gwinyai E. Chibisa
Animals 2024, 14(2), 254; https://doi.org/10.3390/ani14020254 - 13 Jan 2024
Cited by 1 | Viewed by 1983
Abstract
The phenological stage of maturity of grasses and supplementation program can impact forage utilization in grazing beef cattle. However, the potential interaction between harvest maturity of Eragrostis tef (teff) hay and energy supplement source was yet to be fully evaluated. Therefore, our objective [...] Read more.
The phenological stage of maturity of grasses and supplementation program can impact forage utilization in grazing beef cattle. However, the potential interaction between harvest maturity of Eragrostis tef (teff) hay and energy supplement source was yet to be fully evaluated. Therefore, our objective was to determine the effects of harvest maturity of teff hay and supplemental energy sources on nutrient intake, apparent total-tract nutrient digestion, nitrogen (N) utilization, and ruminal fermentation characteristics in beef heifers. A split-plot design with teff hay harvest maturity as the whole plot and supplemental energy source as the subplot was administered in a three-period (21 d), three × three Latin square design. Six crossbred beef heifers (804 ± 53.6 kg of body weight; BW) were allocated to two harvest maturities (early- (EH]) or late-heading (LH)) and to two supplemental energy sources (no supplement (CON), or rolled corn grain or beet pulp pellet fed at 0.5% of BW). Data were analyzed using SAS. There was no harvest maturity × energy supplement interaction. Although harvest maturity had no impact on total dry matter intake (DMI), crude protein (CP) intake was greater (p < 0.01) for EH than LH heifers. Total intakes of dry (DM) and organic matter (OM) were also greater (p < 0.01) for supplemented than CON heifers, whereas acid detergent fiber (ADF) intake was greater for beet pulp heifers compared to heifers fed the CON diet and supplemental corn grain. Harvest maturity had no impact on ruminal pH. However, mean ruminal pH was lower (p = 0.04), duration pH < 6.2, and molar proportions of butyrate and branched-chain fatty acids were greater (p ≤ 0.049) for heifers fed corn grain compared to CON and beet pulp diets. Heifers fed EH hay had greater (p ≤ 0.02) apparent total-tract DM, OM, CP, NDF, and ADF digestibility than heifers fed LH hay. Although there was no supplemental energy effect on microbial nitrogen (N) flow, it was greater (p < 0.01) for EH than LH heifers. Apparent N retention, which did not differ, was negative across all diets. In summary, delaying the harvest of teff hay from the EH to LH stage of maturity compromised nutrient supply, which was not attenuated by feeding supplemental corn grain and beet pulp at 0.5% of diet DM. Because N retention was negative across harvest maturity, there might be a need to provide both energy and protein supplements to improve growth performance when feeding teff hay to beef cattle. Full article
(This article belongs to the Section Animal Nutrition)
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Article
Hydrothermal Treatment via Microwave Radiation Improves Viscoelastic Properties of Native Gluten-Free Flours for Extrusion 3D Printing
by Tetiana Lisovska, Karol Banaś, Agnieszka Orkusz and Joanna Harasym
Appl. Sci. 2023, 13(22), 12465; https://doi.org/10.3390/app132212465 - 17 Nov 2023
Cited by 6 | Viewed by 2019
Abstract
The market for gluten-free products is experiencing significant growth due to the increase in the number of gluten/wheat affected patients diagnosed, but largely as a result of the emergence of a new niche market dedicated for consumers who optionally avoid gluten. Native flours [...] Read more.
The market for gluten-free products is experiencing significant growth due to the increase in the number of gluten/wheat affected patients diagnosed, but largely as a result of the emergence of a new niche market dedicated for consumers who optionally avoid gluten. Native flours are perceived as viable alternative for industrially used starch and hydrocolloids blends, mainly due to their lack of vitamins and minerals, which are abundant in non-refined raw materials. Edible inks for on-demand printing are under significant consideration, while texture building capacity remains an issue. As heat-moisture treatment has proved to be useful for stabilizing the pasting and rheological behavior of various native flours, HMT supported by microwave heating was investigated as printable ink stabilizers. For Spanish and Polish buckwheat flours and two different varieties of teff (white and brown) flour pasting, structural and textural characteristics after the microwave supported heat moisture treatment in 30% of initial moisture content were evaluated. The peak viscosity was reduced by 54% and 60% for Polish and Spanish flour, respectively, while for teff, the reduction was 15% and 43% for the white and brown varieties, respectively. Significant improvement in viscoelastic modulus G’ (for Polish and Spanish buckwheat flour, 32% and 16%, respectively; for white and brown tef varieties, 14% and 18%, respectively) was observed for all the treated samples regardless of the species or variety; this resulted in better performance during 3D printing. Full article
(This article belongs to the Section Applied Biosciences and Bioengineering)
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