Search Results (135)

This study evaluated the effect of chickpea and rice incorporation to replace corn in extruded snacks on protein digestibility, mineral bioaccessibility, and estimated glycemic index (eGI) using a standardized in vitro digestion model (INFOGEST 2.0). Four formulations were analyzed: a control (100% corn, CF) and chickpea-enriched snacks (F30, F60, and the commercial flavored F60 formulation, CCP). Chickpea replacement increased protein, dietary fiber, and mineral contents while reducing total starch. Protein digestibility remained high across all samples (89.5–96.6%), although slightly lower in chickpea/rice-enriched formulations. However, these formulations provided a higher amount of digestible protein because of their increased protein content. Mineral bioaccessibility varied depending on the element, but chickpea and rice incorporation generally enhanced mineral concentrations in the fraction potentially available for absorption despite the presence of phytic acid and insoluble dietary fiber. Starch hydrolysis was rapid during the early stages of digestion, leading to high estimated glycemic index (eGI) values in all samples. Nevertheless, chickpea/rice-enriched formulations exhibited lower eGI values compared to the corn control, likely due to reduced starch content and matrix effects. Overall, chickpea and rice replacement improved the nutritional profile of extruded snacks by enhancing protein quality and mineral availability, despite its impact on glycemic response being limited, highlighting opportunities for further product optimization. Full article

Heavy metal contamination poses significant ecological and public health concerns due to its persistence, toxicity, and bioaccumulative potential of toxic metals, threatening environmental quality and sustainable development. This study evaluated the distribution, pollution status, health risks, and sources of heavy metals in soils from industrial, agricultural, and residential areas of Rivers State, Nigeria, using Inductively Coupled Plasma–Mass Spectrometry (ICP-MS). Elevated concentrations of As, Mn, Se, Zn, and Co were detected across the study locations. Arsenic recorded the highest concentration in the Trans-Amadi industrial area (366.8 mg/kg), while Mn and Zn reached maximum concentrations of 1312 mg/kg and 327.9 mg/kg in Woji and Egi, respectively, exceeding the DPR limits for soil HMs. Pollution indices, including contamination factor, geo-accumulation index, enrichment factor, pollution load index, and Nemerow Integrated Pollution Index, revealed moderate to severe contamination, with the industrial zone exhibiting a NIPI value of 12.5. Health risk assessment identified ingestion as the dominant exposure pathway. Hazard quotient values for As (9.15) in the children category exceeded USEPA-acceptable limits, indicating potential non-carcinogenic risks. Carcinogenic risk assessment identified arsenic as the principal carcinogenic contaminant, with ingestion-derived Incremental Lifetime Cancer Risk (ILCR) values in the industrial area exceeding the USEPA maximum acceptable lifetime cancer risk threshold (1 × 10⁻⁴), particularly among children, whereas the carcinogenic risks associated with the other investigated metals generally remained within acceptable or tolerable limits. Source identification indicated that industrial emissions, vehicular activities, waste disposal, and agricultural practices were the primary contributors to heavy metal accumulation. These findings provide critical scientific evidence for targeted remediation, pollution prevention, and informed health-risk environmental management to protect soil quality, ecosystem integrity, and public health. The study supports evidence-based policies that advance sustainable land management, environmental protection, and progress in Rivers State, Nigeria. Full article

In postmortem Alzheimer’s disease (AD) brains, Porphyromonas gingivalis (Pg), a major periodontal pathogen, and Candida albicans, one of the most common fungal pathogens, have been detected. Although it is important to better understand the effects of their co-infection in the brain for elucidating the pathogenesis of AD, little is known about the neuropathological significance of such co-infection. In the present study, we aimed to elucidate the effects of co-exposure to virulence factors derived from Pg and C. albicans on microglial inflammatory responses. We demonstrated, for the first time, that both candidalysin dissolved in dimethyl sulfoxide (CLd) and water (CLw) significantly suppressed Pg lipopolysaccharide (LPS)-induced interleukin-1β (IL-1β) production by 35–60% and nuclear factor-κB (NF-κB) activation by 20–40%. It should be noted that contaminating Pg outer membrane lipoproteins in Pg LPS were mainly responsible for IL-1β production. To examine the possible hydrophobic interactions between lipoproteins contaminating the Pg LPS preparation and CL, we used 8-anilino-1-naphthalenesulfonic acid sodium salt (ANS-Na), which can be excited to emit fluorescence by binding of hydrophobic molecules. The mean fluorescence intensity of ANS-Na was significantly reduced by approximately 26% following co-treatment with CLw and Pg LPS compared with CLw alone. Furthermore, we generated a mutant form of CL with reduced hydrophobicity (GRAVY index: 1.106 vs. 0.874) while preserving its predicted structural properties. This mutant CLd no longer inhibited Pg LPS-induced IL-1β production. Taken together, these findings indicate that hydrophobic interactions between lipoproteins contaminating the Pg LPS preparation and CL mediate the inhibitory effect of CL on Pg LPS-induced inflammatory responses. The present findings suggest that interactions between polymicrobial virulence factors in the brain may modulate microglia-mediated inflammatory responses during AD progression. Full article

We present a statistical study of low-surface-brightness (LSB) tidal structures in two large samples of edge-on disk galaxies. Our primary sample comprises 5606 galaxies from the Edge-on Galaxies In SDSS (EGIS) catalog, analyzed using imaging from the DESI Legacy Imaging Surveys, supplemented by Hyper Suprime-Cam Subaru Strategic Program (HSCSSP) data and deep Apache Point Observatory (APO) follow-up observations for selected objects. To assess the robustness of our results, we also examine an independent sample of 14,237 galaxies from the Edge-on Galaxies in the Pan-STARRS survey (EGIPS) catalog. All images were processed using a homogeneous procedure optimized for the detection of faint diffuse emission. Tidal structures were identified through visual inspection and classified into established morphological categories, with careful treatment of imaging artifacts and galactic cirrus contamination. We detected tidal features in 324 EGIS galaxies and 690 EGIPS galaxies, corresponding to incidence rates of 5.8% and 4.8%, respectively. Restricting the analysis to completeness-limited subsamples yields consistent fractions of 6.4% and 6.2%. At a typical DESI r-band surface-brightness depth of 28.6 mag arcsec⁻², these values are consistent with previous observational studies but lower than predictions from many cosmological simulations. Recent high-resolution simulations, however, produce incidence rates much closer to those measured here, suggesting that numerical resolution, realistic modeling of observational and instrumental effects, and galaxy formation physics are all critical for accurately predicting the abundance of LSB tidal structures. Full article

Sucrose plays a critical role in fat-continuous confectionery systems such as cocoa–hazelnut spreads by contributing to sweetness, texture, flow behavior, particle interactions, and overall sensory quality. In this study, sucrose was replaced with fructooligosaccharide (FOS) and isomaltooligosaccharide (IMO) at replacement levels of 0, 25, 50, 75, and 100% in cocoa–hazelnut spread formulations. The effects of these substitutions on physicochemical, textural, and rheological properties, particle size distribution, color parameters, estimated glycemic index (eGI), and sensory characteristics were investigated. Sucrose replacement significantly altered the structural and rheological properties of the spread matrix depending on both the type of oligosaccharide and the level of substitution. Formulations containing FOS exhibited more pronounced reductions in firmness and sensory acceptability at higher replacement levels. In contrast, IMO-containing formulations better preserved particle dispersion, viscoelastic stability, and overall sensory quality, particularly at low to moderate substitution levels. However, complete replacement of sucrose showed an adverse effect on taste and spreadability in both oligosaccharide systems. Both oligosaccharides contributed to a reduction in the eGI values. The eGI decreased from 50.71 to 39.90 in FOS-containing formulations and from 51.39 to 41.94 in IMO-containing formulations. These findings suggest that FOS is more effective in lowering the eGI, whereas IMO demonstrates greater potential for maintaining the technological and sensory quality of cocoa–hazelnut spreads when used as a partial sucrose replacer. Full article

This study evaluated the effects of the partial baking (par-baking) method on bread quality, staling properties, volatile compound profile, and estimated glycemic index (eGI) of some traditional Turkish bread types, and compared the results with those of regular bread (RB). Bread samples resembling Afyonkarahisar potato bread and Vakfıkebir bread were produced under laboratory conditions and named Afyonkarahisar potato style bread (APSB) and Vakfıkebir style bread (VKSB). APSB and VKSB were subjected to par-baking (7 min at 220 °C for APSB and 9 min at 220 °C for VKSB), followed by frozen storage at −18 °C for up to 60 days and final baking. Also, quality changes in the breads after final baking were assessed over 5 days (days 0, 1, 3, and 5) and compared with conventionally baked control samples. The reduction in specific volume due to par-baking was more pronounced in regular bread than in the traditional bread samples. Hardness values of par-baked breads increased significantly after 60 days of frozen storage for all bread types (p < 0.05). Storage of breads after final baking caused substantial changes in textural properties, but no significant differences were observed in terms of staling rate among the non-par-baked samples during 5 days of storage at room temperature (p = 0.078). VKSB consistently exhibited the lowest eGI values for both the non-par-baked group and the par-baked group. Principal component analysis (PCA) clearly separated the volatile compound profiles of non-par-baked breads (VKSB-Day 0 and APSB-Day 0) from those of regular bread and par-baked breads. The findings of this study suggest that par-baking technology is a promising approach for breads similar to traditional breads, enabling wider consumption without marked quality deterioration up to 30 days and resulting in a lower estimated glycemic index (eGI) than regular bread. Full article

Resistant starch type 3 (RS3) exhibits physiological benefits in regulating post-meal blood sugar levels and enhancing gut microbiota balance. In this study, mung bean starch was isolated and modified through debranching, annealing (ANN) and heat-moisture treatment (HMT). The multi-scale structures investigated by SEM, FT-IR, and XRD unveiled the formation of short-range ordered, helix, and crystalline structures. Notably, RS3 formed through debranching and HMT exhibited both a remarkably high RS content of 54.71% and a low estimated glycemic index (eGI) of 51.78. Statistical evaluation through correlation and stepwise regression analyses suggested that short-range molecular order was the primary factor associated with the resistance of RS3 to enzymatic hydrolysis, while the chain length of B-chains exerted secondary yet notable influences. This work provided novel insights into the interplay between processing methodologies, ordered molecular structures, and starch digestibility resistance. Full article

Non-fried whole-wheat instant noodles feature high dietary fiber and balanced nutrition but suffer from poor rehydration, texture, and flavor. This study aims to improve the quality of these noodles through mixed fermentation of yeast and lactic acid bacteria (LAB). The rehydration characteristics, textural profile, sensory quality, microstructure, volatile flavor compounds, and in vitro digestibility of non-fried instant whole-wheat noodles were evaluated. Yeast primarily reduced rehydration time and improved mouthfeel, whereas LAB mainly contributed to the formation of a continuous and uniform gluten network as well as the enhancement of noodle flavor. Moderate addition of the mixed culture (1% yeast + 0.05% LAB) promoted the construction of a homogeneous gluten network in non-fried whole-wheat instant noodles, effectively reducing starch digestibility and estimated glycemic index (eGI). However, excessive addition caused opposite effects on these noodles. This study provides an effective processing strategy for the production of high-quality, low-eGI whole-wheat instant noodles, which are suitable for people pursuing healthy diets and controlling blood sugar levels. Full article

This study aimed to evaluate the technological, functional and nutritional effects of exopolysaccharide-producing lactic acid bacteria (LAB) strains, isolated from artisanal Tulum cheese as type II sourdough starters. The objective of this study was to improve bread quality, delay staling, and enhance bioactive properties, such as antioxidant capacity and estimated glycaemic index (eGI). Six LAB strains (Loigolactobacillus coryniformis, Lactiplantibacillus plantarum, Levilactobacillus brevis, Lacticaseibacillus paracasei, Lactobacillus helveticus, and Lacticaseibacillus rhamnosus) were individually used for sourdough fermentation. Bread samples were analyzed for pH, titratable acidity (TA), LAB counts, specific volume, colour, total phenolic content (TPC), antioxidant activity (DPPH and ABTS), starch digestibility, eGI, staling kinetics (Avrami model) and amylopectin retrogradation (DSC). Strain-dependent improvements in bread functionality were observed. L. brevis and L. coryniformis strains increased sourdough acidity to a greater extent, and resulting in lower pH values. Accordingly, bread produced with sourdough fermented by these strains exhibited higher specific volume than the control. Although higher ABTS radical scavenging activity and TPC were detected in sourdough bread compared to the control bread, no significant differences were observed among the breads in terms of total antioxidant activity measured by DPPH. L. rhamnosus significantly improved antioxidant activity and reduced the eGI. L. coryniformis, L. plantarum and L. brevis were the most effective at retarding staling, reducing the increase in hardness and limiting amylopectin retrogradation. This study is the first to demonstrate the functional potential of LAB strains from artisanal Tulum cheese as sourdough starters. These findings reveal the potential for developing clean-label bakery products with an extended shelf life and improved health-related functionality. Full article

Morphine addiction is driven in part by persistent reward-associated memory, yet the molecular mechanisms linking translational control to cellular stress responses remain unclear. In the present study, using a mouse morphine-induced conditioned place preference and N2a cell model, we investigated apoptosis-related alterations in the medial prefrontal cortex and the involvement of eIF4E-dependent translational regulation and BDNF/TrkB signaling. Morphine-induced conditioned place preference was associated with an increase in TUNEL-positive cells in the medial prefrontal cortex, accompanied by upregulation of Bax and downregulation of Bcl-2. In N2a cells, morphine induced apoptosis in a dose-dependent manner. Morphine also increased neuronal eIF4E expression in both mPFC tissue and N2a cells, accompanied by upregulation of BDNF and TrkB. Inhibition of the eIF4E/eIF4G interaction with 4EGI-1 significantly affected morphine-induced CPP formation and altered apoptosis-related markers and BDNF/TrkB expression. Notably, intra-mPFC administration of 4EGI-1 suppressed morphine-induced CPP without affecting anxiety-like behavior, locomotor activity, or general learning and memory performance. These findings suggest that eIF4E-dependent translational regulation is functionally associated with morphine-induced reward memory and apoptosis, potentially in association with changes in BDNF/TrkB molecular expression. This study offers novel insight into the molecular basis of morphine addiction and highlights a potentially targetable translational regulatory pathway for therapeutic intervention. Full article

The valorization of agri-food by-products as functional ingredients requires understanding how processing and formulation affect their nutritional and metabolic properties. This study evaluated the combined effects of drying method (hot air drying, HAD; freeze-drying, FD), particle size (80 and 500 µm), and gum Arabic (GA) addition on the compositional and metabolic functionality of faba bean (Vicia faba L.) pod flour. Proximate composition, total phenolic content (TPC), estimated glycemic index (eGI), glucose dialysis retardation index (GDRI), and enzyme inhibitory activities (α-glucosidase and pancreatic lipase) were determined. Results showed that all factors significantly affected eGI, with independent contributions, whereas GDRI was mainly influenced by particle size and GA, with significant interaction effects. GA addition consistently reduced eGI and increased GDRI, indicating improved modulation of both starch hydrolysis and glucose diffusion. HAD samples showed higher enzyme inhibitory activity, while FD combined with GA enhanced TPC. Particle size modulated structural properties affecting starch accessibility and glucose diffusion. Soluble dietary fiber and phenolic compounds were key contributors to in vitro metabolic functionality, while matrix structure determined their effectiveness. These results suggest that faba bean pod powders may serve as sustainable functional ingredients for food applications, contributing to the valorization of agri-food by-products within a circular economy approach. Full article

This study examines the role of cadastral data quality in shaping risks within investment processes, focusing on the Polish Land and Buildings Register as a key information source for surveying, planning, and design activities. The research is based on an empirical analysis of cadastral data from twelve study areas representing different settlement types, using datasets obtained in 2022 and 2026. Data quality was evaluated in terms of completeness, accuracy, and currency, with particular emphasis on boundary points, land use classifications, and building records. The results reveal significant spatial disparities, with higher data quality observed in large urban areas and lower quality in smaller towns and rural municipalities, where incomplete, inconsistent, or data derived from raster maps are more prevalent. At the same time, a noticeable improvement in data quality was identified over the analysed period, particularly in less urbanised areas. Despite this progress, inconsistencies in attribute definitions, limited standardisation, and gaps in data documentation persist, reducing reliability and interoperability. The findings demonstrate that inadequate cadastral data quality generates legal, technical, temporal, and financial risks, primarily manifested in delays and increased costs due to additional surveying work. A plan has been drawn up to optimise the investor’s activities, taking into account the risks identified during the research. Full article

This study investigated the effects of rice bran extract (RBE) on the quality and digestibility of Chinese steamed buns (CSBs). RBE decreased the starch pasting properties, weakened the starch gel network structure, and reduced the storage modulus (G′) and loss modulus (G″). With the increase in RBE addition in CSBs, lightness difference (ΔL*) decreased, total color difference (ΔE*) increased, and the color of CSBs shifted from light to dark orange-red. Additionally, RBE increased the specific volume and enlarged the pore size of the CSBs. When 15% RBE was added, the CSBs had the lowest hardness and the highest springiness, indicating optimal quality. Notably, the addition of RBE significantly (p < 0.05) reduced the estimated glycemic index (eGI) from 90.916 ± 0.530 to 82.282 ± 0.399 at a 20% concentration, which represents a 9.5% reduction. This study provides a reference for the development of low-glycemic-index (GI) foods. Full article

This paper addresses the growing sustainability fatigue in advanced economies. By analyzing Amazon’s artificial intelligence (AI) agent strategy as a model for “Rational Sustainability”, the study identifies a self-propagating growth trajectory that reconciles economic rationality with value creation. It provides a theoretical and empirical framework to overcome technological saturation and strategic homogenization in the generative AI era. To ensure methodological transparency, the analysis was conducted through two distinct stages: (i) Techno-econometric analysis (macro-level): Using an empirical dataset of 160 countries (40 advanced, 70 emerging, and 50 developing) from 2014 to 2024, the study utilized regression models to quantify the correlations and elasticities between three key proxies: GDP per capita (Y); the Human Capital Index (HCI), representing Institutional Capacity Building (ICB); and the E-Government Development Index (EGI), representing Endogenous Institutional Evolution (EIE). (ii) Hybrid AI analysis (case study): Utilizing process-tracing research, the paper examines Amazon’s R&D structure and AI agent strategy. This qualitative and structural analysis identifies how Amazon co-evolves EIE and ICB to conceptualize tacit knowledge and operationalize it into a competitive advantage. The findings reveal a marked disruption of the co-evolutionary mechanism in advanced economies, where the elasticity of EGI to GDP has declined since 2019, leading to a withdrawal state. In contrast, Amazon’s model demonstrates that the co-evolution of EIE and ICB creates a self-propagating growth engine. This research concludes that “Rational Sustainability”—grounded in evidence, economic rationality, and clear trade-offs—offers a viable pathway for revitalizing sustainability strategies in mature digital economies. Full article

The global ecological conditions are degrading rapidly, and even after the commitment to achieve Sustainable Development Goals by 2030, G7 countries still struggle to meet basic environmental standards. This study offers a novel marginal contribution by analyzing how economic globalization (EG), economic growth intensity (EGI), financial innovation (FI), and environmental technology (ET) influenced environmental degradation (ED) in the G7 countries from 2000 to 2021. Using the Method of Moments Quantile Regression (MMQR) alongside Driscoll and Kraay Standard Error (DKSE), this research provides a first-of-its-kind distributional mapping that accounts for cross-sectional dependency and heterogeneous slopes. Crucially, the findings reveal a “decoupling failure” in advanced economies, where the existing treatment mechanism for ET is insufficient to separate industrial growth from emissions due to institutional discrepancies. While FI is often viewed as a green catalyst, this study identifies it as a “double-edged sword,” showing that it significantly increases environmental degradation in higher quantiles due to carbon-intensive global supply chains. Conversely, EGI is discovered to be mitigatory, suggesting that enhancing financial efficiency and growth soundness can diminish ecological damage. This research fills a critical literature gap by reconciling the Pollution Haven Hypothesis and Green Finance Theory, providing empirical evidence that developed financial systems may inadvertently exacerbate damage if not specifically aligned with green mandates. Full article