Search Results (15)

Old-growth forests are rare in Europe, yet they play a critical role in biodiversity and carbon storage. This study examines the structural dynamics of the Janj old-growth forest in the Dinaric Alps using repeated field measurements from 2011 and 2021 at 39 systematically arranged 12 m radius plots. All trees (DBH ≥ 7.5 cm), regeneration (10 cm height to 7.5 cm DBH), and coarse woody debris (CWD) were assessed. Results revealed that total basal area declined by 3.5 m² ha⁻¹ over the decade, primarily driven by significant reductions in stem density for silver fir (p = 0.001) and Norway spruce (p = 0.001). In contrast, European beech maintained a stable basal area throughout the study period. Moreover, silver fir exhibited a significant increase in mean diameter (p = 0.032) and a pronounced rise in regeneration individuals (t = 3.257, p = 0.002). These findings underscore a gradual compositional shift towards European beech dominance, with conifers facing higher mortality in larger diameter classes. The substantial volume of CWD (463 m³ ha⁻¹) highlights advanced decay dynamics consistent with mature forest conditions. This study emphasizes the value of repeated measurements to capture subtle yet important successional changes in primeval forests, which is essential for conservation planning and sustainable forest management. Full article

Vegetation phenology has lately gained attention in the context of studying human-induced climate change and its effects on terrestrial ecosystems. It is typically studied on various regional and temporal scales. This research focused on the microscale in dolines on the Northernmost part of the Dinaric Alps. The aim was to determine the timing of flowering onset and relate it to topographic and ecological conditions. We studied (1) the floristic gradient along N–W transects divided in 2 m × 2 m plots, from top slopes to the bottom of dolines, and identified discrete groups in relation to this gradient and (2) provided their diagnostic species and communities. The results indicate that the early spring onset of flowering of ground vegetation in the bottom and lower slopes of dolines is stimulated by high spring moisture and nutrient availability, as well as the open canopy of the mesophilous deciduous forests. The flowering onset on the upper slopes and karst plateau starts later, which is due to the precipitation peak in May/June and higher temperatures and light availability of the open canopy of thermophilous deciduous forests. The delayed onset of flowering in late summer in rocky crevices and rocky places is due to a particular physiology stimulated by the harsh site conditions. The phenology pattern along the doline topographic gradient is inverse to general patterns in vegetation phenology. Further study on the role of doline soils should be made to study their impact on phenology. Full article

The mountain forests in Europe, especially the ecosystems dominated by Norway spruce [Picea abies (L.) Karst], are facing major challenges due to climate change. Climatic stress factors such as increased temperatures and drought contribute to reduced growth and increased mortality, especially at lower altitudes. In this study, which was conducted in the northern Velebit region, the growth dynamics and climate sensitivity of Norway spruce were analyzed using standard dendrochronological methods. The focus was on samples collected at altitudes between 1135 and 1545 m. The results show two different growth trends: a positive trend from 1950 to 1977, followed by a negative trend from 1977 to 2013. Precipitation proved to be a key factor for the stability of spruce growth, while the high summer temperatures of the previous year correlated negatively with growth increment. In addition, trees at higher altitudes showed greater resistance to climatic stress. These results underline the crucial role of precipitation and site-specific conditions in maintaining the vitality of spruce forests in mountainous regions, and suggest that climate change could further destabilize spruce ecosystems in the Dinaric Alps. Full article

The aim of this study was to determine the microclimate of brown bear dens depending on their size, their status (active or inactive), and the location of the den. The study included five dens of different sizes and locations in the Velebit Mountains, in the Dinaric Alps. The measurements of air temperature (°C) and relative humidity (%) in dens and the forest stands were carried out over 182 days. The absolute minimum air temperature in inactive dens was between −2.88 °C and −0.38 °C and belonged to the dens with shorter tunnels and without chambers. The exception was inactive den 1, which was situated in a thermophilic forest. No negative absolute minimum air temperatures were recorded in active dens. The absolute minimum air temperatures in the forest stands were lower than in the dens. The relative humidity was significantly higher in all dens than in the forest stands. Dens with a large entrance opening and a short tunnel were strongly influenced by the local microclimate of the forest stand. The greatest temperature difference (den–forest) was between elongated dens with chambers and the associated forest stand, while the smallest temperature differences occurred in dens with a short tunnel, without a chamber, and at the northern exposure. The greatest differences in relative humidity (den–forest) were found in dens with chambers. The den temperatures correlated with the air temperatures in the forest stands. Full article

In the SE Alps, two Cretaceous–Eocene flysch basins, Julian and Brkini, filled with turbidite sediments, are present. This study novelly reports heavy mineral assemblage counts and detrital tourmaline characterization for 11 samples. It is possible to define three different groups, characterized by the presence of (1) a clinopyroxene–epidote–low-ZTR (zircon+tourmaline+rutile; 5%) sample association, (2) a high-ZTR (>48%)–garnet–apatite association and (3) a low-ZTR (<40%)–Cr-spinel–garnet association. Detrital tourmalines from both the Julian and Brkini flysch basins are rather similar in composition, associated with metapelites and metapsammites coexisting or not coexisting with an Al-saturating phase, ferric-iron-rich quartz–tourmaline rocks and calc–silicate rocks; however, their number is drastically different. In fact, even if the percentage of heavy minerals is very low and similar in both basins (0.17–1.34% in weight), in the Julian basin, the number of tourmaline crystals is much lower than that in Brkini (1–14 vs. 30–100), suggesting an important change in the provenance area. Interestingly, the presence of a high amount of tourmaline derived from ferric-iron-rich quartz–tourmaline rocks and calc–silicate rocks makes these two basins different from all the Cretaceous flysch basins of Bosnia and the Northern Dinaric zone, where these supplies are missing or very limited. Full article

Narcissus poeticus L. (Amaryllidaceae), a facultative serpentinophyte, is a highly variable species and particularly important ancestor of cultivated daffodils, but is rarely studied in field populations. This study, based on natural populations in the Balkans, focused on karyotype variability, genome size, ploidy and the presence of B chromosomes. Thirteen native populations from different environmental and soil conditions were collected and analyzed using flow cytometry to estimate nuclear genome size, fluorescence in situ hybridization (FISH) for physical mapping of rDNA, fluorochrome labeling (chromomycin and Hoechst) for heterochromatin organization and silver nitrate staining of nucleoli for determining rRNA gene activity. The organization of rDNA and natural triploids is reported here for the first time. The presence of individuals with B chromosomes (in 9/13 populations) and chromosomal rearrangements was also detected. The observed B chromosome showed three different morphotypes. The most frequent submetacentric type showed four different patterns, mainly with active ribosomal genes. The results obtained show that N. poeticus has a dynamic genome with variable genome size due to the presence of polyploidy, B chromosomes and chromosomal rearrangements. It is hypothesized that the observed changes reflect the response of the genome to different environmental conditions, where individuals with B chromosomes appear to have certain adaptive advantages. Full article

Due to climate extremes and limited natural resources, especially water, we can expect increased demand in the future for species that can better tolerate climate extremes such as drought. One potentially valuable horticultural species is the endemic species of the Dinaride Mountains Micromeria croatica (Pers.) Schott (family Lamiaceae). It grows in the crevices of carbonate rocks, extending from an altitude of 150 m to more than 2000 m. This study aims to provide additional insight into the genetic and morphological diversity of this endemic species, focusing on valuable horticultural traits. To achieve this goal, morphological and molecular analyses were performed on ten natural populations. Through STRUCTURE and PCoA analyses, ten M. croatica populations were placed into western and eastern genetic groups, with several individuals from western populations assigned to the eastern group and vice versa. These atypical individuals assigned to the new genetic group by BAPS analysis indicate gene flow between western and eastern populations. Similarly, an analysis of molecular variance revealed fewer genetic differences than within studied populations. Both PCA and CANDISC analysis based on eleven morphological traits largely confirmed the existence of two slightly different genetic groups. Two populations containing plants with the most flowers per shoot, one with white-flowered individuals, one with the roundest leaves, and one with the narrowest leaves proved to be the most horticulturally valuable. The genetic and morphological variability found should be a sufficient basis for the potential selection of M. croatica populations and individuals for horticultural purposes. Full article

The genus Xerobdella contains three species of land leeches confined to the Palearctic region, one of which is X. anulata (Autrum, 1958), an exceptionally rare endemic of the Dinaric Alps. In the current study, we provide new data and a literature overview on this rarely encountered species, presenting its currently known distribution, providing additional data on its morphology and, for the first time, presenting comprehensive data on its habitat preferences and seasonal dynamics. Additionally, we provide novel DNA barcodes for the Dinaric land leech and compare the obtained sequences with the related X. lecomtei. Altogether, 22 specimens of X. anulata were collected using pitfall traps in three habitat types: managed forests with adjacent meadows and a primeval forest in the Dinaric Alps of Croatia. We report the first finding of X. anulata in open habitats, which harbored most of the specimens. Our findings show that X. anulata exhibits surface activity, highlighting the effectiveness of pitfall traps in sampling such elusive taxa, with perspectives for future morphological, phenological and even molecular research. Full article

The inhabitants of the Dinaric Alps (former Yugoslavia and Albania) have long been known as people of impressive body height, but after World War II, there was a critical lack of data related to this phenomenon. This anthropological synthesis includes the measurements of 47,158 individuals (24,642 males and 22,516 females) from the period 2010–2018 and describes detailed regional differences in male stature in the Western Balkans. According to these data, young men from Montenegro (182.9 cm) are currently the tallest 18-year-olds in the world, surpassing their Dutch peers (182.4 cm), and 18-year-old boys from Dalmatia are even taller (183.7 cm) at a regional level. A continuous belt of extraordinary height means (>184 cm) stretches from the Adriatic coast of Dalmatia through Herzegovina to the central part of Montenegro. This article summarizes all the key socio-economic, nutritional, and genetic data, and offers possible explanations for this anthropological phenomenon. Since the remarkable height of the Dinaric populations cannot be connected with any commonly known environmental factor, the most probable hypothesis is genetic and links these physical characteristics with the local founder effect of Y haplogroup I-M170. Furthermore, given that both the level of socio-economic development and dietary protein quality are still sub-optimal, the local upward trend in body height has the potential to continue in the future. Full article

Springs, as unique ecotonal habitats between surface and hypogean areas, are considered endangered aquatic ecosystems due to direct and indirect human impacts and climate change issues. They are distinctive water habitats that are often inhabited by a diverse but mostly stenotypic group of organisms. The present study considered 31 springs from the Apuseni Mountains (the Romanian Carpathians) that were classified as rheocrene, helocrene, and limnocrene based on their geomorphology and hydrology. Samples from three substrate types (rocks, sand, and bryophytes) were collected using standard methods for crenic invertebrates. A total of 64,462 individuals belonging to 17 invertebrate taxa were identified: aquatic worms, mollusks, crustaceans, water mites, and insects. Amphipoda and Diptera–Chironomidae were the dominant taxa in most springs. At a community level, patterns of habitat preference were demonstrated for 12 invertebrate groups using the standardized selection index (B) and expressed as the number of springs where a certain group selected rocks, sand, and/or bryophytes: Four groups exhibited preferences for bryophytes (Coleoptera, Diptera, Plecoptera, and Trichoptera), Ephemeroptera exhibited preferences for rocks, and Copepoda exhibited preferences for sand. Amphipoda, Platyhelminthes, and Ostracoda displayed preferences for all three substratum types, while Gastropoda, Hydrachnidia, and Oligochaeta recorded lower percentages in springs where habitat preferences were significant. In addition, crenic invertebrates were divided into three guilds, depending on their dispersion abilities in any stage of their life cycle: sedentary (not-winged groups), mobile (winged groups), and ectoparasites (water mites that were able to leave the springs on their winged hosts). Sedentary taxa recorded higher percentages of abundances and habitat preferences towards rocks and sand, while ectoparasites (Hydrachnidia) and the mobile guilds tended to prefer bryophytes. This segregation might be explained by individual adaptations to the particularities of each type of substratum, such as the bodily form of the copepods, which are well suited for sand interstices, a habitat that our data showed that they preferred. Our results represent novel contributions to the knowledge of habitat preferences of spring invertebrates from the Apuseni Mountains, adding value to similar data from the Western Carpathians, the Alps, and the Dinaric region. Full article

The environmental, social and economic potential of Dinaric uneven-aged forests along with the complex stand dynamics influenced by different long-term management approaches and environmental factors require comprehensive forest monitoring. This study aimed to explore differences in the current status and recent past dynamics of stand structures between unmanaged and managed mixed fir-beech forests in the Croatian part of the Dinaric Alps using large-scale data from an established monitoring system. From the 74 permanent sample plots distributed within the forest type measured in 2008 and re-measured in 2019, we stratified four strata (types of management regimes): (1) forests out of regular management and tree harvest for at least 30 years, (2) managed state forests on carbonate bedrock, (3) managed state forests on non-carbonate bedrock and (4) managed private forests. In each sample plot, 34 structural attributes were computed to assess indicators of their current status and recent past dynamics of stands in the studied forests. An increasing Q shape diameter distribution with a high number of large and very large trees characterize unmanaged forests and managed forests on non-carbonate bedrock. In managed state forests and private forests, variable (rotated sigmoid) and constant (negative exponential) results were obtained, respectively. Principal component analysis (PCA) distinguished managed and unmanaged forests with decreasing harvest volume and recruitment, increasing basal area, number of very large trees, average diameter at breast height (DBH), crown defoliation of firs and basal area of died beech trees. The current structure, recent and expected stand dynamics in the unmanaged forests (accumulation of standing volume, increase of large diameter trees and large snags, large share of beech, large mean DBH) can be recognized as old-growth attributes. The differences between the studied forest types, potential of both unmanaged and state managed fir-beech forests and approaches to sustain multifunctional forest management in the Dinaric region were discussed. Full article

Across its pan-European distribution, the European roe deer (Capreolus capreolus) faces a wide diversity of environmental and climatic conditions; therefore, several factors, including intrinsic ones, shape life-history traits and cause significant variability in parameters of fitness. By utilizing microsatellite variations in 214 roe deer females collected throughout Slovenia, Central Europe, we determined the genetic variability and population structure of this species in the contact zone between the Alps and the Dinaric Mountains, i.e., over a wider area where data on the genetic outlook for this—the most common and widespread European wild ungulate—have been completely lacking so far. Throughout the country, we found moderate microsatellite diversity (Ho = 0.57–0.65) in relation to the observed heterozygosity reported for other roe deer populations in Europe. Spatial differences in genetic diversity of the species in Slovenia can be explained by population history linked to varying approaches to population management and/or different connectivity among subpopulations in topographically differentiated habitats. A country-wide pattern of genetic structure is clearly defined by separation of the populations into three groups present in the following regions: (i) Southern sub-Mediterranean and Karst regions, (ii) Central Slovenia, and (iii) the Sub-Pannonian Region in the north-east. This is also confirmed by evidencing a moderate isolation by distance, especially by separating southern samples (coastal Slovenia) from others. Levels of genetic differentiation vary among populations, which can be explained by the effect of natural geographical barriers or the presence of anthropogenic barriers such as urban areas and highways. In the subset of 172 yearling females, we analyzed the influence of genetic advantage (individual heterozygosity) and other genetic data (reflected in the structuring of the population) on body mass and reproductive ability. We found evidence that genetic factors influence the body mass of roe deer yearling females (explaining altogether 18.8% of body mass variance), and the level of individual heterozygosity alone also positively affected body mass, which is in accordance with the theory that heterozygosity is commonly positively correlated with fitness in wild populations. However, we did not uncover any effect of heterozygosity on two parameters of reproductive ability (fertility and potential reproductive outcome), indicating that several other factors, especially environmental ones, have a predominant effect on the parameters of fitness in roe deer. Full article

In the present paper we studied the genetic diversity and genetic structure of five Norway spruce (Picea abies (L.) Karsten) natural populations situated in Serbia, belonging to the southern lineage of the species at the southern margin of the species distribution range. Four populations occur as disjunct populations on the outskirts of the Dinaric Alps mountain chain, whereas one is located at the edge of Balkan Mountain range and, therefore, can be considered as ecologically marginal due to drier climatic conditions occurring in this region. Due to the negative effect of biotic and abiotic stress factors, the sustainability of these populations is endangered, making conservation of their genetic resources one of the key measures of Norway spruce persistence in Serbia under climatic changes. The insight on genetic diversity and genetic structure of the studied spruce populations can provide the information required for the initiation of programs aimed at the conservation and utilization of spruce genetic resources at the rear edge of species environmental limits. Norway spruce genetic variation and population genetic structure were estimated using eight EST-SSR markers. The results showed that mean expected heterozygosity was 0.616 and allelic richness 10.22. Genetic differentiation among populations was low (F_st = 0.007). No recent bottleneck effect or isolation by distance were detected. Bayesian clustering, obtained with STRUCTURE, grouped the populations into two genetic clusters, whereas UPGMA analysis distinguished three main groups approximately in line with the geographic area of occurrence. Based on the study results and the EUFORGEN Pan-European strategy for genetic conservation of forest trees, the establishment of additional dynamic gene conservation units must be considered in Serbia in order to protect the adaptive and neutral genetic diversity of the species. Full article

The gypsy moth, Lymantria dispar, a prominent polyphagous species native to Eurasia, causes severe impacts in deciduous forests during irregular periodical outbreaks. This study aimed to describe the genetic structure and diversity among European gypsy moth populations. Analysis of about 500 individuals using a partial region of the mitochondrial COI gene, L. dispar was characterized by low genetic diversity, limited population structure, and strong evidence that all extant haplogroups arose via a single Holocene population expansion event. Overall 60 haplotypes connected to a single parsimony network were detected and genetic diversity was highest for the coastal populations Croatia, Italy, and France, while lowest in continental populations. Phylogenetic reconstruction resulted in three groups that were geographically located in Central Europe, Dinaric Alps, and the Balkan Peninsula. In addition to recent events, the genetic structure reflects strong gene flow and the ability of gypsy moth to feed on about 400 deciduous and conifer species. Distinct genetic groups were detected in populations from Georgia. This remote population exhibited haplotypes intermediate to the European L. dispar dispar, Asian L. dispar asiatica, and L. dispar japonica clusters, highlighting this area as a possible hybridization zone of this species for future studies applying genomic approaches. Full article

Information about the potential impacts of climate change on river runoff is needed to prepare efficient adaptation strategies. This study presents scenario projections for the future hydrological runoff regime in the Danube River Basin. The eco-hydrological watershed model Soil and Water Integrated Model (SWIM) was applied for the entire Danube River catchment, considering 1224 subbasins. After calibration and validation of the model, a set of high-resolution climate projections (bias-corrected and non-bias-corrected) served as meteorological drivers with which future daily river discharge under different climate warming scenario conditions was simulated. Despite existing uncertainties, robust trends could be identified. In the next 30 years, the seasonal stream-flow regime of the Danube and its tributaries is projected to change considerably. Our results show a general trend towards a decrease in summer runoff for the whole Danube basin and, additionally, in autumn runoff for the Middle and Lower Danube basin, aggravating the existing low flow periods. For the winter and early spring seasons, mainly January–March, an increase in river runoff is projected. Greater uncertainties show up in particular for winter runoff in the Dinaric Alps and the Lower Danube basin. The existing trends become very distinct until the end of the 21st century, especially for snow-influenced river regimes. Full article