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Direct Observation of Protein Microcrystals in Crystallization Buffer by Atmospheric Scanning Electron Microscopy
- Yuusuke Maruyama,
- Tatsuhiko Ebihara,
- Hidetoshi Nishiyama,
- Yuji Konyuba,
- Miki Senda,
- Takuro Numaga-Tomita,
- Toshiya Senda,
- Mitsuo Suga and
- Chikara Sato
X-ray crystallography requires high quality crystals above a given size. This requirement not only limits the proteins to be analyzed, but also reduces the speed of the structure determination. Indeed, the tertiary structures of many physiologically...