Search Results (71)

Quantitative real-time PCR (qPCR) remains a cornerstone method for analyzing gene expression due to its high sensitivity, specificity, and reproducibility. However, for reliable results in relative quantification studies, the choice of an appropriate reference gene is critical to ensure accurate normalization. The expression of commonly used reference genes can vary depending on developmental stage and experimental conditions, making their validation essential. To date, no validated reference genes have been reported for Agrostemma githago L. (corn cockle, Caryophyllaceae). To facilitate research on genes involved in natural product biosynthesis and specialized metabolism regulation, we aimed to identify the most stable reference genes across various plant organs and cultivation conditions of this species. Drawing on previous literature, we have selected seven housekeeping genes widely used for evaluation: actin, β-tubulin, elongation factor 1α, glyceraldehyde-3-phosphate dehydrogenase, histone H3, translation elongation factor 1, and eukaryotic translation initiation factor 5A1 (for which two primer sets were tested). The nucleotide sequences of these potential reference genes were identified from the A. githago transcriptome. Using qRT-PCR, transcript levels of seven potential reference genes were estimated in 40 different A. githago samples, including 25 in vitro samples under various treatment conditions and 15 soil-grown samples representing A. githago organs in different developmental stages. Expression stability of candidate reference genes was assessed using the RefFinder platform, which combines four commonly applied statistical algorithms: geNorm, NormFinder, BestKeeper, and the comparative Δ-Ct method. The results revealed that the selection of optimal reference genes varied based on the particular organ, developmental stage and condition being examined. TIF5A1-2 (one of the two primer pairs tested) and GAPHD consistently exhibited the most stable expression under various conditions in vitro. EF1α and H3 exhibited superior performance across different organs of soil-grown plants. Moreover, our integrated analysis enabled the identification of the two most stable, universal reference genes suitable for normalization in A. githago under all tested conditions—H3 and TIF5A1-2. Our work provides a robust foundation for future transcriptomic and functional studies of the specialized metabolism of A. githago and other related species. Full article

This study presents a taxonomic revision of the genus Dianthus (Caryophyllaceae) in Tunisia, addressing the nomenclatural and morphological complexities of this taxon. Through extensive herbarium research and field investigations, the authors confirm the presence of seven species in Tunisia, including D. cintranus (subsp. byzacenus), D. crinitus, D. illyricus (subsp. angustifolius), D. nudiflorus, D. rupicola (subsp. hermaeensis), D. serrulatus, and D. virgineus. Lectotypes are designated for 11 previously unresolved names, clarifying taxonomic ambiguities. The study challenges existing classifications, particularly for D. serrulatus, where subspecies distinctions are deemed untenable due to overlapping distribution and morphological traits. For D. virgineus, the rank of variety is proposed for the three subspecies present in the country, reflecting the observed morphological diversity. Additionally, six taxa previously reported in Tunisia, such as D. balbisii and D. ferrugineus, are excluded due to a lack of evidence confirming their presence. The research clarifies the nomenclature of taxa previously reported in Tunisia and reports the distribution of species actually found. An identification key is also given. This revision provides a foundational update for future studies on Dianthus in North Africa. Full article

Rangelands form the ecological and economic backbone of Namibia, yet the woody plant dynamics that sustain these landscapes remain sporadically quantified across the semi-arid interior. We investigated the characteristics (stand structure, regeneration, richness, diversity, composition, ecological importance, and indicator species) of woody communities along a pronounced south-to-north rainfall gradient (85–346 mm yr⁻¹) at five representative sites: Warmbad, Gibeon, Otjimbingwe, Ovitoto, and Sesfontein. Field sampling combined point-centered quarter surveys (10 points site⁻¹) and belt transects (15 plots site⁻¹). The basal area increased almost ten-fold along the gradient (0.4–3.4 m² ha⁻¹). Principal Coordinates Analysis (PCoA) arranged plots in near-perfect rainfall order, and Permutational Multivariate Analysis of Variance (PERMANOVA) confirmed significant site differences (F_3,56 = 9.1, p < 0.001). Nanophanerophytes dominated hyper-arid zones, while microphanerophytes appeared progressively with increasing rainfall. Mean annual precipitation explained 45% of the variance in mean height and 34% of Shannon diversity but only 5% of stem density. Indicator value analysis highlighted Montinia caryophyllacea for Warmbad (IndVal = 100), Rhigozum trichotomum (75.8) for Gibeon, Senegalia senegal (72.6) for Otjimbingwe, and Senegalia mellifera (97.3) for Ovitoto. Rainfall significantly influences woody structure and diversity; however, other factors also modulate density and regeneration dynamics. This quantitative baseline can serve as a practical toolkit for designing site-specific management strategies across Namibia’s aridity gradient. Full article

Radix Pseudostellariae (Tai-Zi-Shen), the dried tuberous root of the Caryophyllaceae plant Pseudostellaria heterophylla (Miq.) Pax (P. heterophylla), has been widely used in traditional Chinese medicine (TCM) for thousands of years. It is slightly bitter, neutral in nature, associated with the spleen and lung meridians, and used for nourishing qi, invigorating the spleen, as well as promoting body fluid production and moistening the lungs. In recent years, with the development in natural product chemistry, cyclic peptides, as some of the active constituents derived from P. heterophylla, have gained increasing attention. These cyclic peptides demonstrate a broad range of biological activities, including anticancer, antioxidant, and immunomodulatory effects, as well as cognitive benefits. This review provides an overview of the chemical characteristics and preparation strategies of cyclic peptides from P. heterophylla, and their biological activities and potential mechanisms are also described. The presented work establishes a scientific framework to facilitate the future research and development of P. heterophylla cyclic peptides as potential therapeutic agents. Full article

The high-elevation calcareous screes of the Southern Carpathians are ecologically important habitats characterised by extreme environmental conditions. These habitats support specialised plant communities, including endemic and relict species, shaped by climatic, edaphic and biogeographic factors. This study examines three scree vegetation communities in the Bucegi, Piatra Craiului and Făgăraș massifs to assess species composition, ecological strategies and environmental influences. Phytosociological surveys were carried out using the Braun-Blanquet method, diversity indices (species richness, Simpson indices and species evenness) and multivariate analyses, including ANOSIM (ANalysis Of SIMilarities), SIMPER (Similarity Percentage method) and PCA (Principal Component Analysis), and were applied to evaluate species–environment relationships. A total of 62 vascular plant species were recorded, with Caryophyllaceae and Asteraceae as the dominant families. Differences in lifeform composition and species distribution between the massifs were related to variations in soil moisture, nutrient availability and climatic conditions. The results highlight the role of calcareous substrates in supporting alpine endemism and underline the influence of abiotic stress on community structure. Conservation efforts should prioritise these fragile ecosystems, especially as climate change and human activities increase pressure on high-elevation habitats. The study contributes to a broader understanding of the Carpathian alpine flora and its biogeographic connections with other European mountain systems, and it highlights the need for targeted conservation strategies to preserve biodiversity in these vulnerable environments. Full article

Bayanaul State National Nature Park (BSNNP), which was established in 1985 and is one of the biggest natural parks in the Republic of Kazakhstan, conserves and rehabilitates the natural flora and fauna of the Bayanaul mountain range. This article expands the floristic inventory of BSNNP and identifies the ecological and ethnobotanical importance of the park. The literature revealed that 681 plant species inhabited the BSNNP region but it was hypothesized that the park’s plant diversity was greater than the documented 681 plant species. Following our expedition travels to BSNNP, we extended the flora summary with an addition of 81 new plant species. Now, according to this study, the flora of BSNNP comprises 762 plant species belonging to 335 genera and 81 families. The leading families are Asteraceae Dumort., Poaceae Barnhart, Brassicaceae Burnett, Fabaceae Lindl, Rosaceae Juss., Caryophyllaceae Juss, Lamiaceae Lindl., Apiaceae Lindl., and Scrophulariaceae Juss. They comprise 57.7% of the total plant species in the national park and 58.5% of the total genera. The largest genera are wormwood, sedge, onion, cinquefoil, speedwell, and astragalus, based on which these genera can be considered polymorphic. Moreover, 16 species of endemic plants belonging to 14 genera and 7 families were also reported. The flora is characterized by high biological diversity with the participation of boreal relicts. The largest group among useful species is medicinal plants, represented by 186 species (24.4%) belonging to 83 genera, and 39 families. Our findings enhance the scientific understanding of plant diversity in BSNNP and provide the groundwork for future conservation research. Full article

This study addresses the phylogenetic relationships within the genus Dianthus in South Africa, employing a molecular analysis approach and the distribution pattern of the representatives of the genus. A robust phylogenetic tree comprising three plastids (trnH-psbA, trnK-matK, and trnK-psbA) and a nuclear region was assembled based on 94 taxa of Dianthus and Petrorhagia species using Mr Bayes analysis. Furthermore, distribution maps for each Dianthus species were generated using QGIS. Our results have revealed Dianthus as monophyletic when Petrorhagia is used as an outgroup. Four strongly supported clades were identified (Africa, Eurasian, Verruculosi and Armerium), indicating geographical distinctions. We identified potential discrepancies, notably in the placement of D. mooiensis and D. namaensis. An unexpected relationship between D. thunbergii and D. bolusii was also highlighted, which challenges current morphological classifications. Defined clades were further observed within African Dianthus (Ethiopian and Northern African species; BS/100, and Western and Eastern regions of South Africa; BS/100), while two subclades (BS/100 and BS/72) were observed within the South African clade. Additionally, ITS generated unsupported and unresolved trees independently, as with the three plastid markers (trnH-psbA, trnK-matK, and trnK-psbA). The identified clades and unresolved species placements warrant further investigation, possibly through additional molecular gene markers and broader taxon sampling. Full article

The Caryophyllaceae family, commonly utilized in traditional medicine, exhibits various effects revealed by ethnopharmacological studies. Thus, the diuretic effect of the leaf and stem of three Gypsophila taxa endemic to Türkiye was evaluated for the first time by comparing their bio-metabolic profiles, antioxidant capacities, carbonic anhydrase inhibition, and infrared spectra. The leaf and stem of Gypsophila taxa were macerated in 50% ethanol and 50% water, bio-metabolic profiles were performed by a new validated ultra-performance liquid chromatographic (UPLC) method and spectrophotometric methods, the antioxidant capacity was determined by DPPH and ABTS assays, and the in vitro diuretic activity was evaluated by carbonic anhydrase inhibition. The results show that the G. simonii leaf exhibited the highest quantity of rutin and total polyphenols content (TPC). On the other hand, the G. germanicopolitana leaf showed the highest quantity of rosmarinic acid, and the G. eriocalyx leaf contained the maximum total flavonoids content (TFC). The antioxidant results indicated that G. eriocalyx has the highest capacity. The G. germanicopolitana leaf strongly inhibited the enzyme activity. The ATR-FTIR spectra showed that the general chemical composition in the leaf and stem parts was preserved after the extraction process. Band intensity changes may be due to the extraction process and the amount of substances. In conclusion, the species of Gypsophila taxa show considerable potential for utilization in the pharmaceutical area. Full article

This study investigates the bioactive compound profiles of 12 wild edible plant species from the Amaranthaceae, Apiaceae, Asteraceae, Brassicaceae, Caryophyllaceae, Lamiaceae, Malvaceae, Polygonaceae, and Urticaceae families, consumed as aromatic and medicinal vegetables by local communities and forming part of the natural vegetation of Mount Ergan. The plants were collected and analyzed for their anthocyanin, organic acid, and sugar contents, using advanced liquid chromatography techniques. Statistically significant differences were observed between species for each compound analyzed, highlighting their diverse phytochemical profiles. Malva neglecta, Brassica nigra, and Taraxacum phaleratum exhibited the highest anthocyanin contents, suggesting their strong potential as natural antioxidant sources. Organic acid levels were notably elevated in Polygonum cognatum, T. phaleratum, Urtica dioica, and M. neglecta, which positions these species as promising candidates for use as natural acid regulators in food and pharmaceutical formulations. In terms of sugar content, Chenopodium album, Mentha longifolia, and T. phaleratum had the lowest levels, while M. neglecta, Cirsium arvense, P. cognatum, and Tragopogon buphthalmoides showed significantly higher concentrations, indicating potential applications in the development of natural sweeteners. This study’s findings provide valuable insights into the phytochemical diversity of these wild plant species, emphasizing their potential utility in health nutrition, pharmaceuticals, and cosmetics. This study emphasizes the significance of investigating underexploited plant species for their bioactive chemicals and illustrates their potential contribution to the development of sustainable, natural product-based solutions for diverse industrial uses. Full article

Medicinal plants have long played a crucial role in healthcare systems, but limited genomic information on these species has impeded the integration of modern biological technologies into medicinal plant research. In this study, we selected nine common medicinal plants, each belonging to a different plant family, including Sarcandra glabra (Chloranthaceae), Nekemias grossedentata (Vitaceae), Uraria crinita (Fabaceae), Gynostemma pentaphyllum (Cucurbitaceae), Reynoutria japonica (Polygonaceae), Pseudostellaria heterophylla (Caryophyllaceae), Morinda officinalis (Rubiaceae), Vitex rotundifolia (Lamiaceae), and Gynura formosana (Asteraceae), to estimate their genome sizes and conduct preliminary genomic surveys. The estimated genome sizes by flow cytometry were 3.66 Gb, 0.65 Gb, 0.58 Gb, 1.02 Gb, 3.96 Gb, 2.99 Gb, 0.43 Gb, 0.78 Gb, and 7.27 Gb, respectively. The genome sizes of M. officinalis, R. japonica, and G. pentaphyllum have been previously reported. Comparative analyses suggest that variations in genome size may arise due to differences in measurement methods and sample sources. Therefore, employing multiple approaches to assess genome size is necessary to provide more reliable information for further genomic research. Based on the genome survey, species with considerable genome size variation or polyploidy, such as G. pentaphyllum, should undergo a ploidy analysis in conjunction with population genomics studies to elucidate the development of the diversified genome size. Additionally, a genome survey of U. crinita, a medicinal plant with a relatively small genome size (509.08 Mb) and of considerable interest in southern China, revealed a low heterozygosity rate (0.382%) and moderate repeat content (51.24%). Given the limited research costs, this species represents a suitable candidate for further genomic studies on Leguminous medicinal plants characteristic of southern China. This foundational genomic information will serve as a critical reference for the sustainable development and utilization of these medicinal plants. Full article

A new species of Drymaria from the Kohima District (Nagaland of North-East India) is described and illustrated based on both molecular data (the ITS region of nuclear DNA) and morphometric analyses (clustering, PCA, DA, and MANOVA). The new species resembles D. cordata but differs with respect to the shape of the sepal (oblong, incurved at the margin vs. lanceolate, not incurved at the margin), petals (oblong or linear vs. ovate–lanceolate), and bract (foliaceous, glabrous, non-prominent vs. non-foliaceous, pubescent, and prominent). For nomenclatural purposes, the typification of Drymaria villosa by Duke (in 1961) was corrected according to Art. 9.10 of the Shenzhen Code. Full article

Recruitment poses significant challenges for narrow endemic plant species inhabiting extreme environments like vertical cliffs. Investigating seed traits in these plants is crucial for understanding the adaptive properties of chasmophytes. Focusing on the Iberian endemic genus Petrocoptis A. Braun ex Endl., a strophiole-bearing Caryophyllaceae, this study explored the relationships between seed traits and climatic variables, aiming to shed light on the strophiole’s biological role and assess its classificatory power. We analysed 2773 seeds (557 individuals) from 84 populations spanning the genus’ entire distribution range. Employing cluster and machine learning algorithms, we delineated well-defined morphogroups based on seed traits and evaluated their recognizability. Linear mixed-effects models were utilized to investigate the relationship between climate predictors and strophiole area, seed area and the ratio between both. The combination of seed morphometric traits allows the division of the genus into three well-defined morphogroups. The subsequent validation of the algorithm allowed 87% of the seeds to be correctly classified. Part of the intra- and interpopulation variability found in strophiole raw and relative size could be explained by average annual rainfall and average annual maximum temperature. Strophiole size in Petrocoptis could have been potentially driven by adaptation to local climates through the investment of more resources in the production of bigger strophioles to increase the hydration ability of the seed in dry and warm climates. This reinforces the idea of the strophiole being involved in seed water uptake and germination regulation in Petrocoptis. Similar relationships have not been previously reported for strophioles or other analogous structures in Angiosperms. Full article

The carnation Dianthus fruticosus L. ssp. fruticosus (Caryophyllaceae) is a range-restricted perennial, endemic plant that grows on cliffs, rocks, ravines, terraces, and boulders on Serifos Island in the Cyclades in the Aegean archipelago (Greece), possessing an impressive, aesthetic blossoming during the dry season. This indigenous carnation of Serifos has attracted the interest of naturalists and scientists. Specimens of this subspecific taxon from the island of Serifos (Greece) were collected during botanical explorations in preindustrial times by the French naturalist Joseph Pitton de Tournefort (1702) and in the late eighteenth century by the English Professor of Botany at the University of Oxford John Sibthorp (1787). Those specimens, documented in relevant publications and labelled with different names (i.e., Caryophyllus Graecus arboreus and Caryophyllus arboreus Seriphius in the early eighteenth century and Dianthus fruticosus in the early nineteenth century), are related to aspects of vegetation history, linking the past to the present and (most probably) to the future. Today, a thorough understanding of the in situ development and functionality of this endemic carnation is still required, as is a framework of its sustainability and management in small-scale insular habitats. The timeless perception of the emblematic wild carnation Dianthus fruticosus ssp. fruticosus, which is still growing on inland and coastal sites of Serifos and neighboring islands in the Cyclades (Greece), is also a reminder that a native plant is often a repository to which local communities may look when crafting their identity. Full article

The Black Irtysh River, a major tributary of the Ob River, traverses diverse ecological zones, influencing the distribution and composition of its floodplain vegetation. This study focused on the Black Irtysh River valley, a key segment of the Irtysh basin, to assess the current state of its plant communities. To compile expedition routes and a preliminary floristic list, a critical revision of more than 1000 herbarium sheets was carried out in the herbarium collections of Kazakhstan (Altai Botanical Garden and Institute of Botany and Phytointroduction). During the field season, a study of plant biodiversity was carried out along the entire coastline of the Black Irtysh. As a result, 217 species of higher vascular plants were identified (55% of those previously found in herbarium archives) from 139 genera and 43 families. The habitats of two Red Book species were discovered: Tulipa patens and Tulipa uniflora. It was found that the flora of the Black Irtysh is similar to the flora of the entire Zaisan depression, and families Poaceae, Asteraceae, Amaranthaceae, Caryophyllaceae, Rosaceae, and Fabaceae are predominant. Geobotanical surveys revealed that the species composition of plant communities is poor and similar among survey points. Only the western part of the river delta is characterized by high rates of projective cover and reserves of forage plants. The main factors of anthropogenic influence are fires, livestock grazing, and deforestation. Full article

Xanthones are chemical substances in higher plants, marine organisms, and lower microorganisms. The most prevalent naturally occurring sources of xanthones are those belonging to the families Caryophyllaceae, Guttiferae, and Gentianaceae. Structurally, xanthones (9H xanthan-9-one) are heterocyclic compounds with oxygen and a γ-pyrone component. They are densely packed with a two-benzene ring structure. The carbons in xanthones are numbered from their nucleus and biosynthetic construct. They have mixed shikimate-acetate (higher plants) and acetate-malonate (lower organisms) biosynthetic origins, which influence their classification. Based on the level of oxidation of the C-ring, they are classified into monomers, dimers, and heterodimers. While based on the level of oxygenation or the type of ring residue, they can be categorized into mono-, di-, tri-, tetra-, penta- and hexa-oxygenated xanthones, bis-xanthones, prenylated and related xanthones, xanthonolignoids, and other miscellaneous xanthones. This structural diversity has made xanthones exhibit considerable biological properties as promising antioxidant, antifungal, antimicrobial, and anticancer agents. Structure-activity relationship studies suggest C-1, C-3, C-6, and C-8 as the key positions that influence the biological activity of xanthones. Furthermore, the presence of functional groups, such as prenyl, hydroxyl, glycosyl, furan, and pyran, at the key positions of xanthones, may contribute to their spectrum of biological activity. The unique chemical scaffolds of xanthones, their notable biological activities, and the structure–activity relationships of some lead molecules were discussed to identify lead molecules as possible drug candidates. Full article