Search Results (1,564)

Wildlife rescue centres face considerable challenges in promoting animal welfare and enhancing the care and housing conditions of animals under professional supervision. These challenges are further compounded by the diversity of species admitted, each with distinct specific needs. In Colombia and other Latin American countries, primates are among the most frequently rescued and behaviourally complex mammalian taxa, requiring particular attention. In response, this study aimed to assess the content validity of proposed animal welfare indicators for Cebus albifrons through a Delphi consultation process and to develop two species-specific assessment protocols: a daily-use tool for keepers and a comprehensive protocol for professional audits. A panel of 23 experts in primate care and rehabilitation participated in two consultation rounds to evaluate and prioritise the indicators based on their content validity, perceived reliability, and practicality. Indicators were classified as either animal-based (direct measures) or resource- and management-based (indirect measures). After each round, experts received summarised feedback to refine their responses and facilitate consensus building. Of the 39 initially proposed indicators, 28 were validated for inclusion in the extended protocol and 10 selected for the daily-use checklist. Among these, 20 indicators in the extended protocol and 6 in the daily protocol were resource- or management-based—such as adequate food provision, physical enrichment, and habitat dimensions—highlighting their practical applicability and relevance in identifying welfare issues and risk factors. Although these indirect indicators were more numerous, the top-ranked indicators in both protocols were animal-based, including signs of pain, affiliative behaviours, and abnormal repetitive behaviours. These are essential for accurately reflecting the animals’ welfare state and are therefore critical components of welfare assessment in captive non-human primates. This study demonstrates that welfare assessment tools can be effectively tailored to the specific needs of wildlife rescue centres, providing a robust foundation for enhancing welfare practices. These protocols not only offer practical approaches for assessing welfare but also underscore the importance of embedding animal welfare as a priority alongside conservation efforts. Future research should aim to refine these tools further, assess their implementation, and evaluate inter- and intra-observer reliability to ensure consistency across different settings. Full article

The endangered Bengal slow loris (Nycticebus bengalensis) relies heavily on captive/rescue populations for conservation. This study investigated the critical link between Major Histocompatibility Complex (MHC) class II DRB1 exon 2 (DRB1e2) genetic variation and gut microbiota in 46 captive individuals, aiming to improve ex situ management. Using standardized conditions across three enclosure types, we characterized DRB1e2 polymorphism via targeted sequencing and analyzed fecal microbiota using 16S rRNA gene amplicon sequencing. Results demonstrated that high DRB1e2 polymorphism significantly reduced microbial community evenness. Specific genotypes showed distinct microbial associations: G9 strongly correlated with beneficial short-chain fatty acid producers like Fructobacillus, and G2 positively correlated with Bifidobacterium spp., while G2, G3, and G4 correlated negatively with Buchnera (a nutrient-provisioning symbiont). Genotypes and polymorphism collectively explained 9.77% of microbiota variation, exceeding the weaker (5.15%), though significant, influence of enclosure type on β-diversity. These findings reveal that host DRB1e2 variation is a primary driver shaping gut microbiota structure and taxon abundance in captive slow lorises, providing evidence for MHC-mediated host–microbe co-adaptation. This offers a genetically informed framework for optimizing conservation strategies, such as tailoring diets or probiotics to specific genotypes, to enhance gut health and population viability. Full article

Dugongs (Dugong dugon), classified as vulnerable marine mammals, are increasingly impacted by infectious diseases, yet the role of septicemia and disseminated intravascular coagulation (DIC) in their mortality remains uncharacterized. This study aimed to investigate the pathological and microbiological features associated with an acute mortality event in a juvenile dugong during rehabilitation in southern Thailand. Comprehensive histopathological and microbiological analyses were conducted on tissue samples collected postmortem. Bacterial isolation and identification were performed using standard culture techniques and the VITEK-2 system. Histological examination revealed multisystemic lesions, including fibrin thrombi, hemorrhage, hepatocellular degeneration, pancreatic necrosis, lymphoid depletion, and neuronal damage. Oxidative stress and DNA damage were confirmed in brain tissues through immunofluorescence detection of 4-hydroxynonenal (4-HNE) and 8-hydroxy-2′-deoxyguanosine (8-oxodG). Achromobacter xylosoxidans, an opportunistic pathogen, was isolated from multiple organs, consistent with acute systemic infection. These findings represent the first evidence of septicemia-associated DIC in dugongs caused by A. xylosoxidans, highlighting a previously undocumented cause of mortality in dugongs. The results emphasize the role of opportunistic bacteria in triggering oxidative damage and coagulopathy and underscore the importance of early detection and targeted therapeutic strategies to improve survival in stranded or rehabilitated dugongs. Full article

The captive breeding of marine ornamental fish with specialized larval requirements—such as Chaetodontoplus mesoleucus—remains a major bottleneck in aquaculture, largely due to the lack of techniques tailored to their unique morphological and nutritional needs. The global marine ornamental aquaculture market is valued at approximately USD 2.15 billion annually; however, only around 10% of marine ornamental species are currently supplied through captive breeding, highlighting a substantial technological gap. The artificial propagation of C. mesoleucus is particularly challenging due to the species’ small mouth gape and high nutritional demands during early development. To address this issue, we evaluated the effects of three live-prey types—Euplotes sp., Brachionus sp., and Bestiolina coreana—as well as a mixed diet containing all three, on larval performance. From 3 days post-hatch, larvae were fed each prey type at equal densities (15–20 individuals/mL), and water quality was carefully maintained to minimize external influences. Survival and total length were assessed at 14 dph. At the end of the trial, the mixed-diet group showed the highest survival rate (36.2 ± 5.6%), whereas larvae fed only B. coreana exhibited the greatest total length (7.4 ± 1.2 mm) and a high metamorphosis rate (97.8%). These findings demonstrate that prey selection significantly influences the early survival and growth in C. mesoleucus larvae and highlight the critical role of copepods in promoting growth performance. However, as larval biomass was not quantified, the findings should be interpreted with caution, and future studies incorporating biomass assessments are needed to draw more conclusive inferences. The successful mass rearing of this species supports the feasibility of captive production to reduce wild harvesting, protect coral-reef biodiversity, and promote sustainable ornamental aquaculture. Full article

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae pose a significant public health risk. As zoos grow in popularity, exotic animals come into closer contact with humans, making them potential reservoirs of ESBLs. However, data on ESBL presence in Mexican zoos remains limited. For this reason, this study aimed to isolate and assess the antimicrobial susceptibility of Enterobacteriaceae that colonize avian species in a petting zoo and to identify any ESBL-producing isolates. Cloacal swabs were collected from 34 healthy birds at Miguel Angel de Quevedo Zoo, Veracruz, Mexico. Samples were analyzed microbiologically and molecularly to detect ESBL-encoding genes. A total of seventeen E. coli and one K. pneumoniae strains were isolated from cloacal swabs of bird species, and multidrug resistance (MDR) was found. The most frequently detected genes were bla_CTX-M-1 (16/18) and bla_TEM-1 (12/18). The detection of multidrug-resistant (MDR) strains carrying bla_CTX-M-1, bla_TEM-1, and bla_SHV genes highlights the potential role of birds as reservoirs and disseminators of antimicrobial resistance (AMR) in urban environments. To the best of our knowledge, this is the first study conducted in Mexico. In conclusion, MDR ESBL-producing bacteria were found in the fecal microbiota of bird species at a petting zoo in Mexico. The limitations of this study emphasize the need for a One Health approach to analyze the genome-wide isolates and epidemiology of antimicrobial-resistant bacteria in captive zoo animals in Mexico. This would support targeted surveillance efforts and help reduce the emergence and spread of resistant bacteria among zoo animals and visitors. Full article

Oxytocin (OT) has emerged as a central focus in stress regulation and animal welfare research. While commonly measured in blood, saliva, and urine, its presence in feces remains unexplored. Fecal OT (fOT) could be highly valuable for wildlife studies, where direct handling is impractical. This study explored OT detection in feces using lions (Panthera leo) as a model species. The objectives included developing a reliable fOT extraction protocol, describing baseline fOT patterns in captive lions, and evaluating its relationship with fecal glucocorticoid metabolites (fGM) under non-stressful conditions. Fecal samples from 16 lions in stable social groups across five Spanish zoos were collected over two to six weeks. A methanol-based extraction and commercial enzyme immunoassay were successfully validated for fOT quantification. Concentrations ranged from 3.00 to 296.64 ng OT/g of feces, with notable intra- and inter-individual variability. Age, sex, and contraceptive status had no significant influence on fOT (p > 0.05), but concentrations differed significantly between zoos (p < 0.001). No consistent relationship was found between fOT and fGM levels under baseline conditions. This study is the first to demonstrate OT detection in feces. The validated methodology provides a foundation for investigating fOT as a biomarker of stress and welfare in wild and domestic species. Full article

The welfare of decapod crustaceans held in captivity, whether in aquariums, holding facilities, seafood restaurants, supermarkets, or fishmongers, is attracting growing attention and concern. This review focuses on three species that are commonly found in European commercial environments: the European spiny lobster (Palinurus elephas), the European lobster (Homarus gammarus), and the American lobster (Homarus americanus). We examine the biology and behavioural ecology of these decapods, emphasising their natural habits and environmental requirements. Based on current scientific knowledge, we propose conditions for their confinement that better align with their biological and behavioural needs. These crustaceans are commonly subjected to stressful captive environments characterised by overcrowding, claw immobilisation through banding, an absence of shelter or hiding spaces, and continuous exposure to direct lighting. Such practices contrast starkly with the animals’ innate behaviours and natural habitats, likely compromising their physical health and psychological welfare. It is thereby recommended that spiny lobsters and lobsters be kept separated and stocked at an appropriate density, that each animal should be provided with a shelter and have enough space to turn around, that true lobsters should never have their claws tied, that no direct light should be placed above the aquaria, and that water quality should be routinely monitored. By exploring the welfare challenges associated with confining these species, this review aims to promote a more ethical and humane approach to their care in commercial settings, and to encourage practices that enhance their quality of life in captivity. Full article

Chronic wasting disease (CWD), a transmissible spongiform encephalopathy that targets cervids, has become a significant threat to both free-ranging and captive populations of Canadian white-tailed deer. In an effort to mitigate its spread, research in the past 20 years has demonstrated that the genetic background of deer may influence the pathogenesis of CWD. Specifically, variants located on the 95-, 96-, 116- and 226-codon of the prion protein gene seem to attenuate disease progression in white-tailed deer. The influence of these alleles on the likelihood of being found CWD-positive on Saskatchewan and Albertan farms was assessed using a Bayesian logistic regression model. To assess the presence of selection for favourable prion protein gene alleles, shifts in variant genotype frequencies were examined over the last seventeen years. Our results show that deer harboring the G96S allele have significantly lowered odds of infection within Canadian herds. Furthermore, the prevalence of this allele has increased significantly in farmed deer over the past seventeen years. Establishing the dynamic genetic background of Canadian deer populations will inform future disease management initiatives. Full article

Wine reviews can connect words to flavours; they entwine sensory experiences into vivid stories. This research explores the intersection of artificial intelligence and oenology by using state-of-the-art neural networks to decipher the nuances in wine reviews. For more accurate wine classification and to capture the essence of what matters most to aficionados, we use Hierarchical Attention Networks enhanced with pre-trained embeddings. We also propose an approach to create captivating marketing images using advanced text-to-image generation models, mining a large review corpus for the most important descriptive terms and thus linking textual tasting notes to automatically generated imagery. Compared to more conventional models, our results show that hierarchical attention processes fused with rich linguistic embeddings better reflect the complexities of wine language. In addition to improving the accuracy of wine classification, this method provides consumers with immersive experiences by turning sensory descriptors into striking visual stories. Ultimately, our research helps modernise wine marketing and consumer engagement by merging deep learning with sensory analytics, proving how technology-driven solutions can amplify storytelling and shopping experiences in the digital marketplace. Full article

Background/Objectives: Loss and fragmentation of habitat from agricultural conversion led to the near extirpation of the pygmy rabbit (Brachylagus idahoensis Merriam, 1891) population in the Columbia Basin (CB) of Washington, USA. Recovery efforts began in 2002 and included captive breeding, translocations from other regions for genetic rescue, and reintroduction into native habitat in three sites: Sagebrush Flat (SBF), Beezley Hills (BH), and Chester Butte (CHB). Methods: We used noninvasive and invasive genetic sampling to evaluate demographic and population genetic parameters on three translocated populations of pygmy rabbits over eight years (2011–2020). For each population, our goal was to use fecal DNA sampling and 19 microsatellite loci to monitor spatial distribution, apparent survival rates, genetic diversity, reproduction, effective population size, and the persistence of CB ancestry. Over the course of this study, 1978 rabbits were reintroduced as part of a cooperative conservation effort between state and federal agencies. Results: Through winter and summer monitoring surveys, we detected 168 released rabbits and 420 wild-born rabbits in SBF, 13 released rabbits and 2 wild-born in BH, and 16 released rabbits in CHB. Observed heterozygosity (H_o) values ranged from 0.62–0.84 (SBF), 0.59–0.80 (BH), and 0.73–0.77 (CHB). Allelic richness (AR) ranged from 4.67–5.35 (SBF), 3.71–5.41 (BH), and 3.69–4.65 (CHB). Effective population (N_e) within SBF varied from 12.3 (2012) to 44.3 (2017). CB ancestry persisted in all three wild populations, ranging from 15 to 27%. CB ancestry persisted in 99% of wild-born juveniles identified in SBF. Apparent survival of juvenile rabbits differed across years (1–39%) and was positively associated with release date, release weight, and genetic diversity. Survival of adults (0–43%) was positively influenced by release day, with some evidence that genetic diversity also positively influenced adult apparent survival. Conclusions: Noninvasive genetic sampling has proven to be an effective and efficient tool in monitoring this reintroduced population, assessing both demographic and genetic factors. This data has helped managers address the goals of the Columbia Basin recovery program of establishing multiple sustainable wild populations within the sagebrush steppe habitat of Washington. Full article

Humane euthanasia is an endpoint for production animals succumbing to disease or trauma. Euthanasia performed with barbiturates or other anesthetic/sedative drugs observes zero withdrawal time, and drug residues may remain in tissues. Carcasses may be submitted for rendering, and rendered products can be used to manufacture pet foods. The purpose of this study was to determine the concentration of two drugs, xylazine and ketamine, that may be used during the euthanasia process of food animals and to determine the fate of these drugs following a simulated rendering process using a commercial autoclave. Twelve cattle were administered xylazine or xylazine and ketamine prior to euthanasia via penetrating captive bolt, and samples of muscle, fat, liver, and kidney were collected. The tissue samples were analyzed by LC-MS/MS, both raw and following rendering. The parent compounds xylazine and ketamine were detected in all tissues, both before and after rendering. The highest concentrations were found in rendered kidney for both drugs, and the lowest in rendered and raw fat for xylazine and ketamine, respectively. Full article

The scope and scale of commercial captive lion breeding (CLB) in South Africa have rapidly increased since the 1990s. We conducted a qualitative systematic review using the PRISMA protocol to determine whether CLB provides a sustainable supply side intervention to reduce pressure on wild lion populations. A search was performed using three academic databases for sources published between 2008 and 2023. We collated and reviewed the data using an evaluation framework to determine the potential benefits and threats of CLB in the context of conservation. Among the 126 peer-reviewed and 37 grey literature articles identified, we found evidence suggesting that the framework’s criteria were not fully met, raising concerns that CLB may facilitate the demand for lions, their parts, and derivatives. Our findings further indicate a reasonable cause to doubt that the CLB provides a sustainable supply side intervention to meet the commercial demand for lions, their parts, and derivatives. This could adversely impact conservation of wild lion populations. We conclude that further research is required to effectively evaluate the purported conservation benefits of CLB. These insights may also have implications for the policy and governance of commercial predator breeding operations in South Africa and globally. Full article

Zoological environments aim to promote natural behaviours and optimal welfare conditions. Over the past decade, research on the use of artificial ultraviolet-B (UV-B) exposure has improved vitamin D₃ levels and reduced incidences of metabolic bone disease in diurnal primates; however, this has not been investigated in nocturnals. Aye-ayes (Daubentonia madagascariensis), nocturnal lemurs often housed indoors in zoos with little to no exposure to natural sunlight, have been reported to have low vitamin D₃ levels. This study aims to investigate the impacts of artificial UV-B as a supplemental healthcare strategy for aye-ayes, examining its influences on vitamin D₃ levels and positional sleeping behaviour. The 25-hydroxy-vitamin D₃ (25OHD₃) blood levels were tested before and after exposure to different levels of artificial UV-B and heat sources. Statistical analysis showed no correlation between UV-B and 25OHD₃ at group parameter levels. However, one individual showed a positive correlation. Sleeping position duration analysis showed a potential basking behaviour with the use of increased ear exposure and other thermoregulatory responses. Despite representing 8.06% of the European captive aye-aye population, these findings highlight the need for further research on vitamin D₃ parameters and responses to UV-B to optimise captive conditions and support the species’ long-term health. Full article

Rhabditis (Rhabditella) axei is a predominantly free-living nematode commonly found in sewage systems and decomposing organic matter. While primarily saprophytic, it has been documented as an opportunistic pathogen in human urinary and gastrointestinal tracts. The Chinese red panda (Ailurus styani), a rare and protected species in China, has not previously been reported as a host for Rhabditis (Rhabditella) spp. infections. This study reports the first documented occurrence of R. axei in red panda feces, unambiguously confirmed through integrative taxonomic approaches combining morphological and molecular analyses. The nematodes exhibited key morphological features consistent with R. axei, including a cylindrical rhabditiform esophagus, sexually dimorphic tail structures, and diagnostic spicule morphology. Molecular analysis based on 18S-ITS-28S rDNA sequencing confirmed their identity, showing >99% sequence similarity to R. axei reference strains (GenBank: PP135624.1, PP135622.1). Phylogenetic reconstruction using 18S rDNA and ITS rDNA sequences placed the isolate within a well-supported R. axei clade, clearly distinguishing it from related species such as R. blumi and R. brassicae. The findings demonstrate the ecological plasticity of R. axei as a facultative parasite capable of infecting non-traditional hosts and further highlight potential zoonotic risks associated with environmental exposure in captive wildlife populations. Our results emphasize the indispensable role of molecular diagnostics in accurately distinguishing morphologically similar nematodes within the Rhabditidae family, while providing essential baseline data for health monitoring in both in situ and ex situ conservation programs for this endangered species. Full article

Gaming has evolved into a cultural phenomenon with a global reach, captivating millions of individuals. Nevertheless, little is known about this population. We aim to physiologically characterise the Portuguese gamers, bearing in mind that phase angle (PhA) is a general indicator of health, to check possible correlations between body composition, strength, and nutrition. A sample of 35 male gamers (individuals who play video games) was evaluated for anthropometry; body composition through DXA for whole-body bone mineral content (BMC), fat-free mass (FFM, kg), fat mass, and visceral adipose tissue, and through BIA (bioelectrical impedance analysis) for total body water (TBW), water pools (extracellular water and intracellular water, ICW), and PhA; strength through maximal isometric handgrip strength using a dynamometer; and nutritional intake using a three-day food record. Results show that participants are within reference metrics for all the analysed variables except regarding protein and carbohydrate intake (all values are above and below the Acceptable Macronutrient Distribution Ranges, respectively). A positive correlation was observed between PhA and TBW, ICW, handgrip strength, BMC and FFM, and a negative correlation with fat mass (absolute, percentage and visceral). In conclusion, PhA correlates with body composition variables, which aligns with previous research as a predictor of health and performance. Full article