Search Results (20)

The conversion of CO₂ into value-added chemicals exemplifies an innovative and eco-friendly approach to addressing carbon emissions. In this study, shape-specific CeO₂ nanocrystals (nanorods, nanocubes, and nanoparticles) were successfully synthesized and employed as catalysts to study the structure-dependent behavior and reaction mechanism for one-step CO₂ conversion to diethyl carbonate (DEC). Among the three catalysts, CeO₂ nanorods (Ce-NR) exhibited the best catalytic activity in the synthesis of DEC from CO₂ compared with CeO₂ nanocubes (Ce-NC) and nanoparticles (Ce-NP), which achieved the DEC production of 1.32 mmol_DEC/g_cat at 423 K and 5 MPa for 4 h. Comprehensive characterization further confirmed the enhanced activity of Ce-NR originated from the morphology effect, particularly the promotion of oxygen vacancies and Ce³⁺ species, which promoted reaction activity. Furthermore, the Ce-NR catalyst almost retained 1.32 mmol_DEC/g_cat DEC production of its initial activity after four cycles, underscoring its exceptional stability and promising industrial scalability. These findings provide fundamental insights to guide the rational design of efficient catalysts for CO₂ activation and other critical transformations. Full article

This study presents a comprehensive sustainability assessment of open field strawberry production in Batman, Türkiye, utilizing thermodynamic-based analyses. The inputs used during the production phase were obtained through survey data collected from local farms over the past few growing seasons. This study is the first to provide an integrated energy, exergy, and environmental assessment of open-field strawberry production in the Batman region, offering novel insights into resource efficiency and sustainability evaluation in agricultural systems. The evaluation was conducted for a functional unit of one ton of strawberries by analysing the cumulative energy consumption (CEnC), cumulative exergy consumption (CExC), and cumulative carbon dioxide emissions (CCO₂E). The total CEnC was calculated to be 1207.8 MJ/ton, with nitrogen fertilizer (390.91 MJ/ton) and diesel fuel (229.67 MJ/ton) being the most significant inputs. The total CExC was 1919.82 MJ/ton, where farmyard manure (1170.19 MJ/ton) was the dominant contributor, accounting for approximately 61% of the total. The total carbon footprint (CCO₂E) was determined to be 41.43 kg/ton, with irrigation water (10.19 kg/ton) and farmyard manure (10.14 kg/ton) being the primary sources. Key sustainability indicators, the Cumulative Degree of Perfection (CDP) and Renewability Indicator (RI), were calculated as 1.34 and 0.26, respectively. The CDP value is notably higher than that of other open field strawberry studies, while the low RI value indicates a heavy reliance on non-renewable resources. The findings highlight that improving sustainability requires a focus on efficient manure management and reducing fossil fuel-based energy consumption. Full article

Human corneal endothelial cell therapy has recently emerged as a novel solution to treat corneal endothelial diseases. We previously demonstrated the potential of utilizing non-cultured primary corneal endothelial cells (CEnCs) isolated from donor corneas with low endothelial cell density for simple non-cultured endothelial cell injection (SNEC-I) therapy. This study aimed to develop a robust and semi-automated approach for cell counting, characterize the extent of cellular manipulation, and evaluate the translational workflow. To address this, we evaluated manual and automated cell counting approaches and characterized the extent of manipulation of CEnCs through the analysis of cell cycle status, gene expressions, and transcriptomic profiles with single-cell RNA-sequencing. The translational feasibility and functionality of SNEC-I therapy were examined using an established rabbit model of bullous keratopathy. Manual hemocytometry and automated cell-counters exhibited comparable accuracy and reproducibility. Analysis of cell cycle status, cell cycle genes (n = 11), and transcriptomic profiles revealed close resemblance between the native corneal endothelium and its donor-matched SNEC-I-harvested cells. Successful resolution of bullous keratoplasty in the pre-clinical model supports the feasibility, efficacy, and safety of SNEC-I therapy. In conclusion, SNEC-I therapy serves as an attractive corneal endothelial therapeutic approach (from a regulatory standpoint) in view of the minimal extent of cellular manipulation. Full article

Fuchs Endothelial Corneal Dystrophy (FECD) is a corneal endothelial disease that causes microenvironment alterations and endothelial cell loss, which leads to vision impairment. It has a high global prevalence, especially in elderly populations. FECD is also one of the leading indications of corneal transplantation globally. Currently, there is no clearly defined canonical pathway for this disease, and it has been proposed that the combinatorial effects of genetic mutations and exogenous factors cause FECD. Clinical studies and observations have provided valuable knowledge and understanding of FECD, while preclinical studies are essential for gaining insights into disease progression and mechanisms for the development and testing of regenerative medicine therapies. In this review, we first introduce the proposed genetic and molecular pathologies of FECD. Notably, we discuss the impact of abnormal extracellular matrix deposition (guttata), endothelial-to-mesenchymal transition, cell senescence, and oxidative stress on the pathology and etiology of FECD. We review and summarize the in vitro cell models, ex vivo tissues, and in vivo animal models used to study FECD. The benefits and challenges of each model are also discussed. Full article

Fuchs endothelial corneal dystrophy (FECD) is a progressive and debilitating disorder of the corneal endothelium (CE) that affects approximately 4% of individuals over the age of 40. Despite the burden of the disease, the pathogenesis of FECD remains poorly understood, and treatment options are limited, highlighting the need for deeper investigation into its underlying molecular mechanisms. Over the past decade, studies have indicated independent contributions of endoplasmic reticulum (ER) and mitochondrial stress to the pathogenesis of FECD. However, there are limited studies suggesting ER-mitochondria crosstalk in FECD. Recently, our lab established the role of chronic ER stress in inducing mitochondrial dysfunction for corneal endothelial cells (CEnCs), indicating the existence of ER-mitochondria crosstalk in FECD. This paper aims to provide a comprehensive overview of the current understanding of how ER and mitochondrial stress contribute to FECD pathogenesis. The paper also reviews the literature on the mechanisms of ER-mitochondria crosstalk in other diseases relevant to FECD. Full article

The Pattern Informatics Method (PI) was initially developed for medium-to-long-term earthquake prediction by analyzing changes in seismic activity. It has since been refined and extended to identify ionospheric anomalies associated with earthquakes. Notable advancements include the development of modified and improved methods, which have demonstrated their capability to detect significant short-term and ionospheric anomalies preceding earthquake events. In this study, the IPI method was applied to infrared satellite observation data for the first time, and a new algorithm for extracting short-term and imminent anomalies from infrared earthquakes was explored based on the IPI method, from which we obtained the MIPI (Modified Improved Pattern Informatics Method). Using 1° × 1° nighttime Outgoing Longwave Radiation (OLR) data from NOAA_18 satellites of the National Oceanic and Atmospheric Administration’s Climate Prediction Center (NOAA-CPC) of the United States, the evolution of OLR anomalies before the Ridgecrest Ms 6.9 earthquake in the United States on 6 July 2019 as recorded by the China Earthquake Networks Center (CENC) and the Maduo Ms 7.4 earthquake in China on 21 May 2021 as recorded by the China Earthquake Networks Center (CENC) were studied. In order to make the IPI method suitable for the calculation of OLR data, two modifications were made to the IPI algorithm: (1) the quartile method was applied for automatically determining the abnormal changes in the OLR observation data and they were used as the input data instead of ionospheric data; (2) the standard deviation of the multi-year OLR residual data of each grid was used instead of the maximum anomaly index used in the original method to re-assign and obtain the relative anomaly index, and finally the anomaly evolution time series diagram was drawn. The results show the following: (1) The MIPI method can effectively extract short-term and imminent OLR anomalies prior to earthquakes. (2) Short-term and imminent OLR anomalies appeared about two weeks before each earthquake and lasted until the earthquake occurrence, disappearing after the earthquake. During this process, the anomalies exhibited a certain evolutionary trend. (3) The short-term and imminent OLR anomalies prior to each earthquake were distributed near the epicenter or near the seismogenic fault, about 200 KM away from the epicenters. The above results are similar to the spatiotemporal evolution characteristics of seismic infrared short-term anomalies previously studied, which indicates that the MIPI method can effectively extract seismic infrared anomalies and might provide a practical method for the extraction of seismic infrared short-term and imminent anomalies. Full article

The objective of this study is to enhance the resilience of the coal-to-liquids (CTL) industrial chain and supply chain to withstand increasing shock pressures. There is an urgent need to improve the resilience of the industrial chain and supply chain. This paper identifies 21 resilience-influencing factors from 4 perspectives: absorption capacity, adaptability, recovery capacity, and self-learning capacity; it then constructs an evaluation indicator system. The Interval Type 2 Fuzzy-Decision-Making Trial and Evaluation Laboratory-Analytic Network Process (IT2F-DEMATEL-ANP) method is adopted to determine the weights of the indicator system, and a resilience evaluation is performed based on the Interval Type 2 Fuzzy-Prospect Theory-Technique for Order Preference by Similarity to an Ideal Solution (IT2F-PT-TOPSIS) method. Furthermore, in the case of the CTL industrial chain and supply chain of China Shenhua Energy Group Ningxia Coal Industry Co., Ltd. (CENC) (Ningxia, China), this study ranks the resilience level from 2018 to 2022 to identify the factors that have contributed to a reduction in resilience and to implement measures to enhance the resilience of the CTL industrial chain and supply chain. The results show that the level of the CTL industrial chain and supply chain resilience was lowest in 2020, while it was highest in 2021. Factors such as the degree of domestication of key technologies, the rationality of the CTL industry layout, and the stability of supply and demand chains are identified as significant determinants of resilience levels. This points the way to enhancing the resilience of the CTL industry and supply chain. Full article

Type 2 diabetes mellitus (T2DM) as a chronic metabolic disease has become a global public health problem. Insulin resistance (IR) is the main pathogenesis of T2DM. Oxidative stress refers to an imbalance between free radical production and the antioxidant system, causing insulin resistance and contributing to the development of T2DM via several molecular mechanisms. Besides, the reduction in hepatic glycogen synthesis also leads to a decrease in peripheral insulin sensitivity. Thus, reducing oxidative stress and promoting glycogen synthesis are both targets for improving insulin resistance and treating T2DM. The current study aims to investigate the pharmacological effects of single-atom Ce-N-C nanozyme (SACe-N-C) on the improvement of insulin resistance and to elucidate its underlying mechanisms using HFD/STZ-induced C57BL/6J mice and insulin-resistant HepG2 cells. The results indicate that SACe-N-C significantly improves hepatic glycogen synthesis and reduces oxidative stress, as well as pancreatic and liver injury. Specifically, compared to the T2DM model group, fasting blood glucose decreased by 29%, hepatic glycogen synthesis increased by 17.13%, and insulin secretion increased by 18.87%. The sod and GPx in the liver increased by 17.80% and 25.28%, respectively. In terms of mechanism, SACe-N-C modulated glycogen synthesis through the PI3K/AKT/GSK3β signaling pathway and activated the Keap1/Nrf2 pathway to alleviate oxidative stress. Collectively, this study suggests that SACe-N-C has the potential to treat T2DM. Full article

Pt supported on carbon (Pt/C) is deemed as the state-of-the-art catalyst towards oxygen reduction reactions (ORRs) in chemical and biological fuel cells. However, due to the high cost and scarcity of Pt, researchers have focused on the development of Earth-abundant non-precious metal catalysts, hoping to replace the traditional Pt/C catalyst and successfully commercialize the chemical and biological fuel cells. In this regard, electrocatalysts made of transition metals emerged as excellent candidates for ORRs, especially the electrocatalysts made of Fe and Co in combination with N-doped carbons, which produce potentially active M-N₄-C (M=Co, Fe) ORR sites. At present, however, the transition metal-based catalysts are popular; recently, electrocatalysts made of rare earth metals are emerging as efficient catalysts, due to the fact that rare earth metals also have the potential to form rare earth metal-N₄-C active sites, just like transition metal Fe-N₄-C/Co-N₄-C. In addition, mixed valance states and uniqueness of f-orbitals of the rare earth metals are believed to improve the redox properties of the catalyst that helps in enhancing ORR activity. Among the rare earth metals, Ce is the most interesting element that can be explored as an ORR electrocatalyst in combination with the N-doped carbon. Unique f-orbitals of Ce can induce distinctive electronic behavior to the catalyst that helps to form stable coordination structures with N-doped carbons, in addition to its excellent ability to scavenge the OH^● produced during ORRs, therefore helping in catalyst stability. In this study, we have synthesized Ce/N-C catalysts by a metal–organic framework and pyrolysis strategy. The ORR activity of Ce/N-C catalysts has been optimized by systematically increasing the Ce content and performing RDE studies in 0.1 M HClO₄ electrolyte. The Ce/N-C catalyst has been characterized systematically by both physicochemical and electrochemical characterizations. The optimized Ce/N-C-3 catalyst exhibited a half-wave potential of 0.68 V vs. RHE. In addition, the Ce/N-C-3 catalyst also delivered acceptable stability with a loss of 70 mV in its half-wave potential when compared to 110 mV loss for Pt/C (10 wt.%) catalyst, after 5000 potential cycles. When Ce/N-C-3 is used as a cathode catalyst in dual-chamber microbial fuel cells, it delivered a volumetric power density of ~300 mW m⁻³, along with an organic matter degradation of 74% after continuous operation of DCMFCs for 30 days. Full article

Descemet’s Stripping Only (DSO) is a surgical technique that utilizes the peripheral corneal endothelial cell (CEnC) migration for wound closure. Ripasudil, a Rho-associated protein kinase (ROCK) inhibitor, has shown potential in DSO treatment; however, its mechanism in promoting CEnC migration remains unclear. We observed that ripasudil-treated immortalized normal and Fuchs endothelial corneal dystrophy (FECD) cells exhibited significantly enhanced migration and wound healing, particularly effective in FECD cells. Ripasudil upregulated mRNA expression of Snail Family Transcriptional Repressor (SNAI1/2) and Vimentin (VIM) while decreasing Cadherin (CDH1), indicating endothelial-to-mesenchymal transition (EMT) activation. Ripasudil activated Rac1, driving the actin-related protein complex (ARPC2) to the leading edge, facilitating enhanced migration. Ex vivo studies on cadaveric and FECD Descemet’s membrane (DM) showed increased migration and proliferation of CEnCs after ripasudil treatment. An ex vivo DSO model demonstrated enhanced migration from the DM to the stroma with ripasudil. Coating small incision lenticule extraction (SMILE) tissues with an FNC coating mix and treating the cells in conjunction with ripasudil further improved migration and resulted in a monolayer formation, as detected by the ZO-1 junctional marker, thereby leading to the reduction in EMT. In conclusion, ripasudil effectively enhanced cellular migration, particularly in a novel ex vivo DSO model, when the stromal microenvironment was modulated. This suggests ripasudil as a promising adjuvant for DSO treatment, highlighting its potential clinical significance. Full article

More than 75% of traumatic brain injuries (TBIs) are mild (mTBI) and military service members often experience repeated combat-related mTBI. The chronic comorbidities concomitant with repetitive mTBI (rmTBI) include depression, post-traumatic stress disorder or neurological dysfunction. This study sought to determine a long noncoding RNA (lncRNA) expression signature in serum samples that correlated with rmTBI years after the incidences. Serum samples were obtained from Long-Term Impact of Military-Relevant Brain-Injury Consortium Chronic Effects of Neurotrauma Consortium (LIMBIC CENC) repository, from participants unexposed to TBI or who had rmTBI. Four lncRNAs were identified as consistently present in all samples, as detected via droplet digital PCR and packaged in exosomes enriched for CNS origin. The results, using qPCR, demonstrated that the lncRNA VLDLR-AS1 levels were significantly lower among individuals with rmTBI compared to those with no lifetime TBI. ROC analysis determined an AUC of 0.74 (95% CI: 0.6124 to 0.8741; p = 0.0012). The optimal cutoff for VLDLR-AS1 was ≤153.8 ng. A secondary analysis of clinical data from LIMBIC CENC was conducted to evaluate the psychological symptom burden, and the results show that lncRNAs VLDLR-AS1 and MALAT1 are correlated with symptoms of depression. In conclusion, lncRNA VLDLR-AS1 may serve as a blood biomarker for identifying chronic rmTBI and depression in patients. Full article

Transparency of the human cornea is responsible for clear vision, which is maintained by a monolayer of non-proliferative human corneal endothelial cells (HCEnCs). Dysfunction of these cells can result in irreversible corneal blindness. It is important to identify key factors that limit the proliferation of HCEnCs and thus attempt to reverse them. Extracellular vesicles contain cargo which includes microRNAs (miRNAs) that can modulate a cellular function. In non small cell lung cancer, expression of miR-195-5p has been shown to inhibit proliferation; therefore, we aimed to investigate the inhibitory effect of miR-195-5p in inducing the proliferation of HCEnCs. Human corneal endothelial cell line (HCEC-12) and primary HCEnCs were cultured with miR-195-5p scramble, mimic or inhibitor. Corneal tissues from human cadaveric and FECD donors, and from pigs, mice and rabbits, were used for RT-PCR. miR-195-5p showed an abundance value of 11,363.31 a.u. When normalized against HCEnCs from cadaveric donors, FECD tissues showed a significant upregulation of miR-195-5p (p < 0.05) but was significantly downregulated in pig (p < 0.001), mouse (p < 0.01) and rabbit (p < 0.001) CEnCs, which have known proliferative capacity. Proliferation, cell doubling, and wound healing rates were significantly higher when miR-195-5p was inhibited. Inhibiting miR-195-5p showed a significant improvement in viability (HEC staining), decreased cell apoptosis (TdT-dNTP staining) and expression of ZO-1, NA⁺/K⁺-ATPase and Ki-67 markers. Expression of miR-195-5p is found in HCEnCs and FECD cells, which restricts the proliferation of these cells. However, inhibiting miR-195-5p can induce the proliferation of HCEnCs, which opens exciting directions for future research in prolonging FECD pathogenesis by increasing the proliferative capacity of HCEnCs using anti-miR therapy in vivo. Full article

Human corneal-endothelial cells (hCEnCs) are located on the inner layer of the cornea. Injury to CEnCs leads to permanent corneal edema, requiring corneal transplantation. NADPH oxidase 4 (NOX4) has been reported to be implicated in the pathogenesis of CEnCs diseases. Thus, we investigated the role of NOX4 in CEnCs in this study. In an animal study, siRNA for NOX4 (siNOX4) or plasmid for NOX4 (pNOX4) was introduced into the corneal endothelium of rats by electroporation, using a square-wave electroporator (ECM830, Havard apparatus) to decrease or increase the expression of NOX4, respectively, and the rat corneas were cryoinjured through contact with a metal rod of 3 mm diameter frozen in liquid nitrogen for 10 min. The immunofluorescence staining of NOX4 and 8-OHdG showed that the levels of NOX4 and 8-OHdG were decreased in the siNOX4 group compared to the siControl, and increased in the pNOX4 group compared to the pControl at one week after treatment. Without cryoinjury, corneal opacity was more severe, and the density of CEnCs was lower, in pNOX4-treated rats compared to pControl. After cryoinjury, the corneas were more transparent, and the CEnC density was higher, in siNOX4-treated rats. The hCEnCs were cultured and transfected with siNOX4 and pNOX4. The silencing of NOX4 in hCEnCs resulted in a normal cell shape, higher viability, and higher proliferation rate than those transfected with the siControl, while NOX4 overexpression had the opposite effect. NOX4 overexpression increased the number of senescent cells and intracellular oxidative stress levels. NOX4 overexpression increased ATF4 and ATF6 levels, and nuclear translocation of XBP-1, which is the endoplasmic reticulum (ER) stress marker, while the silencing of NOX4 had the opposite effect. Additionally, the mitochondrial membrane potential was hyperpolarized by the silencing of NOX4, and depolarized by NOX4 overexpression. The LC3II levels, a marker of autophagy, were decreased by the silencing of NOX4, and increased by NOX4 overexpression. In conclusion, NOX4 plays a pivotal role in the wound-healing and senescence of hCEnCs, by modulating oxidative stress, ER stress, and autophagy. The regulation of NOX4 may be a potential therapeutic strategy for regulating the homeostasis of CEnCs, and treating corneal-endothelial diseases. Full article

The existence of aftershocks in an earthquake sequence can impact the analysis of the mainshock. In this study, we present a method for deleting an aftershock sequence based on the spatial relationship between earthquakes and faults. This method improves the performance of space window selection in the classical K-K method by eliminating aftershocks with an ideal fault buffer zone. The determination of fault buffer zones is based on a trial-and-error analysis of 69,714 earthquake records from the China Seismic Network Center (CENC) collected between 1980 and 2020. We selected 20 typical big earthquakes ($M_L$7.0–8.0 or $~Ms$6.6–8.0; for earthquakes above magnitude $Ms$7 or $M_{L}7.2$, $M_L$ is approximately equal to $Ms$) as the mainshocks to establish the fault buffer zones. We also propose an empirical formula to determine the distance of the fault buffer zone by counting the aftershock deletion effect at different buffer distances. Compared with the classical K-K method, our method considers the correlation between the spatial distribution of aftershocks and faults, eliminates earthquake groups that are not related to the mainshock, greatly reduces the spatial range of aftershocks, improves the performance of deleting aftershocks of different magnitudes, and provides a new rule and reference for aftershock deletion. Full article

The loss or dysfunction of human corneal endothelial cells (hCEnCs) is a leading cause of blindness due to corneal failure. Corneal transplantation with a healthy donor cornea has been the only available treatment for corneal endothelial disease. However, the need for way to regenerate the CEnCs has been increased due to the global shortage of donor corneas. The aim of the study is to investigate whether novel Rho-kinase (ROCK) inhibitors can induce the cultivation and regeneration of hCEnCs. Cultured hCEnCs were treated with Y-27632, sovesudil, or PHP-0961 for 24 h. Cellular responses, including cell viability, cytotoxicity, proliferation, and Ki67 expression with ROCK inhibitors were evaluated. We also evaluated wound healing and cell adhesion assays. Porcine corneas were used ex vivo to evaluate the effects of Y-27632, sovesudil, and PHP-0961 on wound healing and regeneration. We performed live/dead cell assays and immunofluorescence staining for SRY (sex determining region Y)-box 2 (SOX2), β-catenin, and ZO-1 on porcine corneas after ROCK inhibitor treatments. Cell viability, cell proliferation rate, and the number of Ki67-positive cells were higher in Y-27632, sovesudil and PHP-0961 treated cells compared to the control. There was no difference in LDH cytotoxicity test between any groups. Cells treated with Y-27632, sovesudil and PHP-0961 showed faster migration, wound healing, and cell adhesion. In the porcine ex vivo experiments, wound healing, the number of live cells, and SOX2-positive cells were higher in Y-27632, sovesudil and PHP-0961 treated corneas. In all experiments, sovesudil and PHP-0961, the novel ROCK inhibitors, were equal or superior to the results of the ROCK inhibitor positive control, Y-27632. In conclusion, sovesudil and PHP-0961, novel ROCK inhibitors have the capacity to regenerate hCEnCs by enhancing cell proliferation and adhesion between cells. Full article