Search Results (1,981)

The development of active food packaging has evolved rapidly in recent years, offering innovative solutions to enhance food preservation and safety while addressing sustainability challenges. This review compiles and analyzes recent advancements (2019–2024) in release-type active packaging, focusing on essential oils, natural extracts, and phenolic compounds as active agents. Primarily plant-derived, these compounds exhibit significant antioxidant and antimicrobial activities, extending shelf life and enhancing food quality. Technological strategies such as encapsulation and polymer blending have been increasingly adopted to overcome challenges related to volatility, solubility, and sensory impact. Integrating bio-based polymers, including chitosan, starch, and polylactic acid, further supports the development of environmentally friendly packaging systems. This review also highlights trends in compound-specific research, release mechanisms, and commercial applications, including a detailed analysis of patents and case studies across various food matrices. These developments have already been translated into practical applications, such as antimicrobial sachets for meat and essential oil-based pads for fresh produce. Moreover, by promoting the valorization of agro-industrial by-products and the use of biodegradable materials, emission-type active packaging contributes to the principles of the circular economy. This comprehensive overview underscores the potential of natural bioactive compounds in advancing sustainable and functional food packaging technologies. Full article

Occupational heat stress represents an increasing challenge to safety and operational performance in underground mining, where elevated temperatures, humidity, and limited ventilation are common. This study proposes an integrated framework to analyze the systemic impact of heat stress on human reliability in mining operations. We conducted a systematic literature review to identify empirical studies addressing thermal exposure, extracting key operational functions for modeling. These functions were structured using the Functional Resonance Analysis Method (FRAM) to reveal interdependencies and performance variability. Human reliability was evaluated using Fuzzy CREAM, which quantified the degree of contextual control associated with each function. Finally, we applied the Gaussian Analytic Hierarchy Process (AHP) to prioritize functions based on thermal impact, contextual reliability, and systemic connectivity. The results showed that functions involving subjective or complex judgment, such as assessing thermal stress or identifying psychophysiological indicators, exhibited lower reliability and higher vulnerability. In contrast, monitoring and control functions based on standardized procedures were more stable and resilient. This combined approach identified critical points of systemic fragility and offers a robust decision-support tool for prioritizing thermal risk mitigation. The findings contribute to advancing the scientific understanding of heat stress impacts in mining and support the development of targeted interventions to enhance human performance and safety in extreme environments. Full article

China is one of the countries severely affected by earthquakes, making precise and timely identification of earthquake precursors essential for reducing casualties and property damage. A novel method is proposed that combines a rock acoustic emission (AE) detection technique with deep learning methods to facilitate real-time monitoring and advance earthquake precursor detection. The AE equipment and seismometers were installed in a granite tunnel 150 m deep in the mountains of eastern Guangdong, China, allowing for the collection of experimental data on the correlation between rock AE and seismic activity. The deep learning model uses features from rock AE time series, including AE events, rate, frequency, and amplitude, as inputs, and estimates the likelihood of seismic events as the output. Precursor features are extracted to create the AE and seismic dataset, and three deep learning models are trained using neural networks, with validation and testing. The results show that after 1000 training cycles, the deep learning model achieves an accuracy of 98.7% on the validation set. On the test set, it reaches a recognition accuracy of 97.6%, with a recall rate of 99.6% and an F₁ score of 0.975. Additionally, it successfully identified the two biggest seismic events during the monitoring period, confirming its effectiveness in practical applications. Compared to traditional analysis methods, the deep learning model can automatically process and analyse recorded massive AE data, enabling real-time monitoring of seismic events and timely earthquake warning in the future. This study serves as a valuable reference for earthquake disaster prevention and intelligent early warning. Full article

A newly emerging disease affecting Schizolobium parahyba (commonly known as pachaco), termed “decline and dieback,” has been reported in association with the fungal pathogens Fusarium sp. and Botryodiplodia sp. This study assessed the antagonistic potential of two Trichoderma sp. isolates (CEP-01 and CEP-02) against these phytopathogens under controlled laboratory conditions. The effects of three temperature regimes (5 ± 2 °C, 24 ± 2 °C, and 30 ± 2 °C) on the growth and inhibitory activity of two Trichoderma spp. isolates were evaluated using a completely randomized design. The first experiment included six treatments with five replicates, while the second comprised twelve treatments, also with five replicates. All assays were conducted on PDA medium. No fungal growth was observed at 5 ± 2 °C. However, at 24 ± 2 °C and 30 ± 2 °C, both isolates reached maximum growth within 72 h. At 24 ± 2 °C, both Trichoderma spp. isolates exhibited inhibitory activity against Fusarium sp. FE07 and FE08, with radial growth inhibition percentages (RGIP) ranging from 37.6% to 44.4% and 52,8% to 54.6%, respectively. When combined, the isolates achieved up to 60% inhibition against Fusarium sp., while Botryodiplodia sp. was inhibited by 40%. At 30 ± 2 °C, the antagonistic activity of Trichoderma sp. CEP-01 declined (25.6–32.4% RGIP), whereas Trichoderma sp. CEP-02 showed increased inhibition (60.3%–67.2%). The combination of isolates exhibited the highest inhibitory effect against Fusarium sp. FE07 and FE08 (68.4%–69.3%). Nonetheless, the inhibitory effect on Botryodiplodia sp. BIOT was reduced under elevated temperatures across all treatments. These findings reinforce the potential of Trichoderma spp. isolates as a viable and eco-friendly alternative for the biological control of pathogens affecting S. parahyba, contributing to more sustainable disease management practices. The observed inhibitory capacity of Trichoderma sp., especially under optimal temperature conditions, highlights its potential for application in integrated disease management programs, contributing to forest health and reducing reliance on chemical products. Full article

This work investigated the ion exchange technique for selective separation of rare earth elements (REE) from acid mine drainage (AMD), using different column systems, pH values, and eluent concentrations. Systematic analysis of pH and eluent concentration showed that an initial pH of 6.0 and 0.02 mol L⁻¹ NH₄EDTA are the optimal conditions, achieving 98.4% heavy REE purity in the initial stage (0 to 10 bed volumes). This represents a 32-fold increase compared to the original AMD (6.7% heavy REE). The speciation of REE and impurities was determined by Visual Minteq 4.0 software using pH 2.0, which corresponds to the pH at the inlet of the fractionation column. Under this condition, La and Nd and the impurities (Ca, Mg, and Mn) remained in the fractionation column, while Al was partially retained. In addition, the heavy REE (Y and Dy) were mainly in the form of REE-EDTA complexes and not as free cations, which made fractionation more feasible. The fractionation column minimized impurities, retaining 100% of Ca and 67% of Al, generating a liquor concentrated in heavy REE. This sustainable approach adopted herein meets the critical needs for scalable recovery of REE from diluted effluents, representing a circular economy strategy for critical metals. Full article

The aim of this study was to isolate Weizmannia spp. that produce lactic acid from xylose and use an experimental design to optimize the production of the metabolite. After isolation, the experiments were conducted in xylose-yeast extract-peptone medium. The identification of isolates was performed using the 16S rDNA PCR technique, followed by sequencing. A central composite rotatable design (CCRD) was used to optimize the concentrations of the carbon source (xylose), nitrogen source (yeast extract and peptone), and sodium acetate. Two strains were considered promising for lactic acid production, with W. coagulans BLMI achieving greater lactic acid production under anaerobic conditions (21.93 ± 0.9 g.L⁻¹) and a yield of 69.18 %, while the strain W. ginsengihumi BMI was able to produce 19.79 ± 0.8 g.L⁻¹, with a yield of 70.46 %. CCRD was used with the W. ginsengihumi strain due to the lack of records in the literature on its use for lactic acid production. The carbon and nitrogen sources influenced the response, but the interactions of the variables were nonsignificant (p < 0.05). The response surface analysis indicated that the optimal concentrations of carbon and nitrogen sources were 32.5 and 3.0 g.L⁻¹, respectively, without the need to add sodium acetate to the culture medium, leading to the production of 20.02 ± 0.19 g.L⁻¹, productivity of 0.55 g/L/h after 36 hours of fermentation, and a residual sugar concentration of 12.59 ± 0.51 g.L⁻¹. These results demonstrate the potential of W. ginsengihumi BMI for the production of lactic acid by xylose fermentation since it is carried out at 50 °C, indicating a path for future studies Full article

Extensive beef systems in the tropics are the cheapest but require more land and longer rearing times with environmental impact. This study was carried out to evaluate three beef bull’s production systems in tropics: pasture-based system (PASTU), feedlot system immediately after weaning (FELOT) and a system with the combination of rearing in pasture and finishing in feedlot (PRIME) on animal performance and carcass characteristics of 30 bulls crossbred Angus x Nellore. The final weight, average daily gain and carcass weight (hot and cold) were higher (p < 0.050) for the FELOT system, intermediate for the PRIME system and lowest for the PASTU system. The carcass dressing (hot and cold), dripping losses, ratio (Longissimus dorsi) and degree of finishing were similar (p > 0.050). The carcass pH_24h was higher for the PRIME system (p < 0.010). Subcutaneous fat thickness (mm) was lower for the PASTU system (p < 0.050). Marbling was better for the PRIME system. The tissular composition was similar among systems related to muscle percentage but PASTU showed the highest bone percentage (p < 0.050) and lowest of adipose (p < 0.050). PRIME enable cost-effective, fast beef production with less environmental impact. Full article

Stevia rebaudiana Bertoni, a semi-perennial herb from the Asteraceae family, is native to the Paraguay–Brazil border region. The growing industrial interest in this species is due to its natural sweetening properties, such as steviol and its derivatives, which offer sweetness without adding calories. Morphological traits are crucial for assessing genetic variability and ensuring distinctness, homogeneity, and stability (DHS) for cultivar protection. This study characterized 19 elite Stevia genotypes from Embrapa Cerrados’ Active Germplasm Bank (BAG) using 21 morphological descriptors from Brazil’s Ministry of Agriculture, Livestock, and Supply (MAPA). Genetic distances were calculated using the simple coincidence index complement method, and clustering was performed via the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). The results showed that 17 of the 21 descriptors (>80%) effectively differentiated the genotypes, revealing significant genetic variability. Dendrogram analysis identified at least four major similarity groups, highlighting the potential of these genotypes for Stevia breeding programs. These findings underscore the suitability of these elite genotypes for developing superior varieties adapted to Cerrado conditions, supporting future cultivation and genetic improvement efforts. Full article

Nonspecific toxicity to normal and malignant cells restricts the clinical utility of many anticancer drugs. In particular, anemia in cancer patients develops due to drug-induced toxicity to red blood cells (RBCs). The anticancer alkaloid, cepharanthine (CEP), elicits distinct forms of cell death including apoptosis and autophagy, but its cytotoxicity to RBCs has not been investigated. Colorimetric and fluorometric techniques were used to assess eryptosis and hemolysis in control and CEP-treated RBCs. Cells were labeled with Fluo4/AM and annexin-V-FITC to measure Ca²⁺ and phosphatidylserine (PS) exposure, respectively. Forward scatter (FSC) was detected to estimate cell size, and extracellular hemoglobin along with lactate dehydrogenase and aspartate transaminase activities were assayed to quantify hemolysis. Physiological manipulation of the extracellular milieu and various signaling inhibitors were tested to dissect the underlying mechanisms of CEP-induced RBC death. CEP increased PS exposure and hemolysis indices and decreased FSC in a concentration-dependent manner with prominent membrane blebbing. Although no Ca²⁺ elevation was detected, chelation of intracellular Ca²⁺ by BAPTA-AM reduced hemolysis. Whereas SB203580, D4476, acetylsalicylic acid, necrosulfonamide, and melatonin inhibited both PS exposure and hemolysis, staurosporin, L-NAME, ascorbate, caffeine, adenine, and guanosine only prevented hemolysis. Interestingly, sucrose had a unique dual effect by exacerbating PS exposure and reversing hemolysis. Of note, blocking KCl efflux augmented PS exposure while aggravating hemolysis only under Ca²⁺-depleted conditions. CEP activates Ca²⁺-independent pathways to promote eryptosis and hemolysis. The complex cytotoxic profile of CEP can be mitigated by targeting the identified modulatory pathways to potentiate its anticancer efficacy. Full article

This study investigates the potential of entomopathogenic nematodes (EPNs) as biological control agents by exploring their occurrence and diversity in Amazcala, Querétaro. The aim was to characterise their distribution and evaluate their pathogenicity against insect pests. Soil samples were collected from various agricultural lands, followed by laboratory isolation and the molecular identification of EPN species. Morphological and genetic analyses confirmed the presence of several species with distinct pathogenic profiles. Pathogenicity assays using the larval stages of Galleria mellonella and Tenebrio molitor revealed that Heterorhabditis bacteriophora and Heterorhabditis atacamensis exhibited significant virulence, with Galleria mellonella being more susceptible. Among the 12 recovered EPN isolates, three strains—AMZX05 (Heterorhabditis atacamensis), AMZX10 (Heterorhabditis bacteriophora), and AMZX13 (Heterorhabditis atacamensis)—demonstrated particularly high pathogenic potential. These strains represent promising candidates for biological control and could contribute to sustainable integrated pest management (IPM) strategies. Further research is recommended to optimise their application across diverse agroecosystems. Full article

Climate change and landscape fragmentation have made fires the primary drivers of forest degradation in Southern Amazonia. Understanding their impacts is crucial for informing public conservation policies. In this study, we assessed the effects of repeated fires on trees with a diameter ≥10 cm across three distinct vegetation types in this threatened region: Amazonian successional forest (SF), transitional forest (TF), and ombrophilous forest (OF). Two anthropogenic fires affected all three vegetation types in consecutive years. We hypothesized that SF would be the least impacted due to its more open structure and the presence of fire-adapted savanna (Cerrado) species. As expected, SF experienced the lowest tree mortality rate (9.1%). However, both TF and OF were heavily affected, with mortality rates of 28.0% and 29.7%, respectively. Despite SF’s apparent fire resilience, all vegetation types experienced a significant net loss of species and individuals. These results indicate a fire-induced degradation stage in both TF and OF, characterized by reduced species diversity and structural integrity. Our findings suggest that recurrent fires may trigger irreversible vegetation shifts and broader ecosystem tipping points across the Amazonian frontier. Full article

The aim of this study was to determine, through meta-analysis, the effects of malic acid/malate addition on ruminal and blood parameters and diet digestibility in cattle. The literature search was conducted in Web of Science, Science Direct, and Google Scholar using the terms “organic acids”, “malic acid”, “malate”, and “bovine”. The database was composed of papers published between 1980 and 2023. The average effect of malate/malic acid inclusion was calculated using the “DerSimonian and Laird” random effects model. Meta-regression and subgroup analyses were conducted to explore sources of heterogeneity. Overall, malic acid (MAC) addition did not significantly affect rumen pH (ES = 0.310, p = 0.17), but subgroup analysis showed that malate increased pH (ES = 1.420, p < 0.01). MAC increased rumen propionate (ES = 0.560, p < 0.01) and total volatile fatty acids (VFAs; ES = 0.508, p = 0.03), while reducing the acetate-to-propionate ratio (p < 0.01). Starch and NDF intake were significant covariates affecting pH and VFA-related variables. MAC improved total-tract digestibility of dry matter (DM; ES = 0.547, p ≤ 0.05), crude protein (CP; ES = 0.422, p ≤ 0.05), and acid detergent fiber (ADF; ES = 0.635, p ≤ 0.05). It increased glucose levels (Overall ES = 0.170, p = 0.05) and reduced NEFA (Overall ES = −0.404, p = 0.03). In conclusion, the effectiveness of MAC depends on its chemical form. Improvements in rumen pH, fiber degradation, and blood parameters suggest more efficient energy use and potential metabolic benefits. The influence of diet-related covariates suggests that the response to MAC may vary depending on the nutritional composition of the diet. Full article

Background: Diabetes and cancer are two of the most life-threatening disorders affecting individuals of all ages worldwide. This study aimed to develop a novel Acrocomia aculeata (bocaiuva) fruit pulp oil-loaded nanoemulsion and evaluate its inhibitory effects on α-glucosidase and pancreatic lipase, as well as its antiglycant activity and cytotoxicity against cancer cells. Additionally, this study assessed the impact of both the oil and the nanoemulsion on blood cells. Methods: The pulp oil was extracted by cold pressing. The oil’s physicochemical properties were determined according to the AOAC and the Brazilian Pharmacopeia. The lipid profile was performed by GC-MS. The nanoemulsion was prepared by the phase inversion method using ultrasonic stirring for particle size reduction and for homogenization. Response Surface Methodology was used for optimizing nanoemulsion preparation. Enzyme inhibition tests were conducted using assay kits. Cytotoxicity in cancer cells was evaluated using the Sulforhodamine B assay. Results: Comprehensive physicochemical and chemical characterization of bocaiuva oil was performed, identifying oleic acid (71.25%) as the main component. The oil contains 23.04% saturated fatty acids, 73.79% monounsaturated acids, and 3.0% polyunsaturated fatty acids. The nanoemulsion (particle size 173.6 nm; zeta potential −14.10 mV) inhibited α-glucosidase (IC₅₀: 43.21 µg/mL) and pancreatic lipase (IC₅₀: 41.99 µg/mL), and revealed a potent antiglycation effect (oxidative IC₅0: 18.36 µg/mL; non-oxidative pathway IC₅₀: 16.33 µg/mL). The nanoemulsion demonstrated good cytotoxicity and selectivity against prostate cancer cells (IC₅₀: 19.13 µg/mL) and breast cancer cells (IC₅₀: 27.22 µg/mL), without inducing hemolysis, platelet aggregation, or anticoagulant effects. Conclusions: In this study, a comprehensive physical and chemical characterization of bocaiuva fruit pulp oil was conducted for the first time as a preliminary step toward its future standardization as an active ingredient in cosmetic and pharmaceutical formulations. The resulting nanoemulsion represents a novel alternative for managing diabetes and cancer. Although the nanoemulsion exhibited lower cytotoxicity compared to doxorubicin, it remains promising due to its composition of essential fatty acids, phenols, and carotenoids, which offer multiple health benefits. Further studies are needed to validate its efficacy and safety in clinical applications. Full article

Sperm flagellum defects are tightly associated with male infertility. Centriolar satellites are small multiprotein complexes that recruit satellite proteins to the centrosome and play an essential role in sperm flagellum biogenesis, but the precise mechanisms underlying this role remain unclear. Serologically defined colon cancer autoantigen protein 8 (SDCCAG8), which encodes a protein containing eight coiled-coil (CC) domains, has been associated with syndromic ciliopathies and male infertility. However, its exact role in male infertility remains undefined. Here, we used an Sdccag8 mutant mouse carrying a CC domains 5–8 truncated mutation (c.1351–1352insG p.E451GfsX467) that models the mutation causing Senior–Løken syndrome (c.1339–1340insG p.E447GfsX463) in humans. The homozygous Sdccag8 mutant mice exhibit male infertility characterized by multiple morphological abnormalities of the flagella (MMAF) and dysmorphic structures in the sperm manchette. A mechanistic study revealed that the SDCCAG8 protein is localized to the manchette and centrosomal region and interacts with PCM1, the scaffold protein of centriolar satellites, through its CC domains 5–7. The absence of the CC domains 5–7 in mutant spermatids destabilizes PCM1, which fails to recruit satellite components such as Bardet–Biedl syndrome 4 (BBS4) and centrosomal protein of 131 kDa (CEP131) to satellites, resulting in defective sperm flagellum biogenesis, as BBS4 and CEP131 are essential to flagellum biogenesis. In conclusion, this study reveals the central role of SDCCAG8 in maintaining centriolar satellite integrity during sperm flagellum biogenesis. Full article

This article examines the persistent conceptual and structural obstacles that pluralism faces within the Euro-United-Statesian art system, particularly in the fields of criticism, art history, and aesthetics. The study situates its inquiry within broader debates around the politics of difference and the decolonization of knowledge, aiming to understand how theoretical frameworks historically incorporated plurality in ways that ultimately neutralize its disruptive potential. Methodologically, the article combines philosophical analysis with a critical rereading of canonical texts by figures such as Clement Greenberg and Arthur Danto, juxtaposed with insights from Indigenous, Black, and decolonial thinkers. The findings suggest that pluralism, while rhetorically embraced, is frequently rendered compatible with a teleological and universalizing narrative that privileges Western aesthetic trajectories. As a result, forms of difference are tolerated only insofar as they can be translated into hegemonic terms. The article concludes by advocating for critical practices that sustain rather than resolve difference, calling for frameworks capable of embracing dissonance, incommensurability, and multiple ontologies without collapsing them into sameness. In doing so, it repositions the contemporary struggle over meaning in art not as a problem to be overcome, but as a necessary symptom of epistemic plurality. Full article