Search Results (234)

Verticillium longisporum, a soil-borne fungus responsible for Verticillium wilt, primarily colonizes members of the Brassicaceae family. Using Arabidopsis thaliana roots as an experimental host, we systematically identify V. longisporum-responsive genes and pathways through comprehensive transcriptomic analysis, alongside screening of potential hub genes and evaluation of infection-associated regulatory mechanisms. The GSE62537 dataset was retrieved from the Gene Expression Omnibus database. After performing GEO2R analysis and filtering out low-quality data, 222 differentially expressed genes (DEGs) were identified, of which 184 were upregulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed on these DEGs. A protein–protein interaction network was constructed using the STRING database. CytoHubba and CytoNCA plugins in Cytoscape v3.10.3 were used to analyze and evaluate this network; six hub genes and four functional gene modules were identified. The GeneMANIA database was used to construct a co-expression network for hub genes. Systematic screening of transcription factors within the 14 DEGs revealed the inclusion of the hub gene NAC042. Integrative bioinformatics analysis centered on NAC042 enabled prediction of a pathogen-responsive regulatory network architecture. We report V. longisporum-responsive components in Arabidopsis, providing insights for disease resistance studies in Brassicaceae crops. Full article

Bee pollen’s aroma combined with other floral components serve various purposes, including attracting pollinators and signaling the availability of food sources. The present study aimed to comparatively analyze the volatile profiles of unifloral pollen taxa. Bee pollen loads were collected using pollen traps and sorted based on their botanical origin, determined by color and pollen grain morphology. The separated pollen samples were analyzed using a Purge & Trap/GC-MS system, identifying the volatile profiles of pollen from 16 plant species. The analysis revealed distinguished differences in the total volatile organic compounds (VOCs) among the various pollen species. Notably, the pollen from Erica manipuliflora, Papaver rhoeas, and Sisymbrium irio contained the highest number of VOCs, with 54, 51, and 42 substances detected, respectively. Certain volatile compounds appeared to correlate with increased bee visitation. For instance, 4-methyl-5-nonanone was uniquely found in E. manipuliflora pollen, while isothiocyanate compounds were exclusively present in species of the Brassicaceae family. Therefore, given the significant impact of VOCs on honey bees’ preferences, it is essential to consider not only the nutritional value of bee pollen when evaluating its beekeeping value, but also its aroma profile. Full article

Camelina sativa (L.) Crantz, a native European oilseed crop of the Brassicaceae family, is notable for its short life cycle, making it well-suited for crop rotation and diversification. Its seeds contain a high content of oil (36–47%) that is rich in polyunsaturated fatty acids (PUFAs) such as alpha-linolenic acid (ALA, C18:3, Ω-3) and linoleic acid (LA, C18:2, Ω-6). This oil has diverse industrial applications, including low-emission biofuels, animal feed, pharmaceuticals, biolubricants, bioplastics, and cosmetics. We analyzed the expression of seven key enzymes involved in fatty acid biosynthesis across nine C. sativa accessions at three stages of silique development using highly efficient qRT-PCR assays designed for the target genes and a normalizing control. Our detailed expression analysis revealed significant variation across varieties, with only the gene FAB2c exhibiting genotype-independent expression, indicating a constitutive and essential role in monounsaturated fatty acid (MUFA) biosynthesis. Other genes showed significant interactions between the variety and developmental stage, highlighting the combined influences of genetic background and silique maturation on gene regulation. V18 emerges as particularly promising, exhibiting elevated expression of genes linked to PUFA and VLCFA biosynthesis—traits of significance for food, biofuel, and industrial applications. These findings, together with the developed qRT-PCR assays, provide valuable tools for selecting Camelina varieties with optimized genetic profiles, highlighting the potential of harnessing natural transcriptional diversity for crop improvement. Full article

In recent years, sustainable agricultural practices in wheat cultivation have garnered significant attention, particularly those focused on minimizing pesticide and herbicide usage to safeguard the environment. One effective approach is green manuring, which entails rotating wheat with crops such as soybean and mustard to harness their natural pesticidal and herbicidal properties. While this method presents clear environmental advantages, it also poses a risk of cross-contamination, as these globally recognized allergens may unintentionally pass through wheat-based products. To protect consumers with allergies, there is an urgent need for a reliable analytical method to detect and quantify these allergenic proteins in wheat-derived foodstuffs. In this study, we assessed various protein extraction protocols to optimize the recovery of soybean and mustard allergens from wheat flour. The extracted proteins were analyzed using a bottom-up proteomics approach involving trypsin digestion, coupled with reversed-phase liquid chromatography and mass spectrometry in multiple reaction monitoring (MRM) mode. Two key allergenic proteins, Glycinin G1 and 11S Globulin, were selected as representative for soybean and mustard, respectively. The identified quantifier marker of Glycinin G1 was VLIVPQNFVVAAR (m/z 713.431²⁺), while FYLAGNQEQEFLK (m/z 793.896²⁺) and VFDGELQEGR (m/z 575.280²⁺) were designated as qualifier markers. The selection of specific marker peptides for mustard proved challenging due to the high structural similarity among proteins from Sinapis alba and other members of the Brassicaceae family. For 11S Globulin, FNTLETTLTR (m/z 598.319²⁺) was recognized as the quantifier marker, with VTSVNSYTLPILQYIR (m/z 934.019²⁺) serving as the qualifier marker. The developed method underwent thorough validation for linearity, limit of detection (LOD), limit of quantification (LOQ), recovery, repeatability, and reproducibility, as well as potential matrix and processing effects. This strategy successfully facilitated the identification and quantification of soybean and mustard allergenic proteins in complex, processed food matrices, including naturally contaminated flour and cookies. These findings enhance food safety monitoring and regulatory compliance, thereby helping to mitigate allergen-related risks in wheat-based products. Full article

Background. Worldwide, colorectal cancer is the third most commonly diagnosed cancer. It is the second leading cause of cancer-related mortality. Recent studies establish a relationship between natural compounds from plants with the prevention and treatment of cancer. Specifically, glucosinolates with antitumoral capacity and polyphenols with the ability to scavenge free radicals that can cause cell damage have been identified in the Brassicaceae family. Objectives. Based on the previously mentioned factors, this study aimed to develop a nutraceutical made with extracts from different Brassicaceae seeds and study its antioxidant and antiproliferative action in vitro and in vivo using the AOM/DSS model in CC57BL6J mice. Results. Extract from the seeds of Eruca sativa and Sinapis alba showed the highest antioxidant capacity among the different species studied and were selected for nutraceutical formulation, which was potentially absorbable (73%) after an in vitro digestion process. In total, thirty compounds were identified in the nutraceutical that could be responsible for its antioxidant and tumoral prevention capacity. The intake of nutraceutical was a successful intervention to prevent the development of polyps by 31.6% and their size by 53.9%. When the nutritional intervention was used in combination with a physical exercise protocol, these parameters dropped to 52.3% and 62.6%, respectively. Conclusions. These findings suggest that the consumption of a diet rich in bioactive compounds from Brassica species, in combination with physical activity, is a valuable prevention strategy for colorectal cancer. However, more research is required to evaluate the efficacy and safety of these interventions in clinical settings. Full article

Phytoalexins are specialized metabolites that are synthesized by plants in response to pathogens. A paradigm in transcription factor (TF) biology is that conserved TFs have dedicated roles across plant lineages in regulating specific branches of specialized metabolism. However, the Arabidopsis (Arabidopsis thaliana) NAC family TF ANAC042 (a.k.a. JUNGBRUNNEN1 or JUB1) regulates the synthesis of camalexin, a Trp-derived phytoalexin specifically produced by several Brassicaceae species, whereas its homolog in soybean (Glycine max) regulates the synthesis of glyceollins, which are Phe-derived phytoalexins specific to soybean. The question addressed by this research is whether ANAC042 broadly regulates phytoalexin biosynthetic pathways in Arabidopsis. Using a novel matrix-assisted laser desorption ionization high-resolution mass spectrometry (MALDI-HRMS) method, we found that the Arabidopsis loss-of-function mutant anac042–1 elicited with bacterial flagellin (Flg22) is deficient in lineage-specific Trp- and conserved Phe-derived phytoalexins—namely camalexin and 4-hydroxyindole-3-carbonyl nitrile (4OH-ICN), and pathogen-inducible monolignols and scopoletin, respectively. Overexpressing ANAC042 in the anac042-1 mutant restored or exceeded wildtype amounts of the metabolites. The expression of phytoalexin biosynthetic genes in mutant and overexpression lines mirrored the accumulation of metabolites. Yeast-one hybrid and promoter-reporter assays in Nicotiana benthamiana found that the ANAC042 protein directly binds and activates the promoters of CYP71B15, CYP71A12, and PAL1 genes for the synthesis of camalexin, 4OH-ICN, and pathogen-inducible monolignol/scopoletin, respectively. Our results demonstrate that ANAC042 regulates conserved and lineage-specific phytoalexin pathways in Arabidopsis. The latter suggests that it is an opportunistic TF that has coopted lineage-specific genes into phytoalexin metabolism, thus providing an exception to the current paradigm. Full article

In the present study, the sequencing and analysis of the complete chloroplast genome of Cleome houtteana and its comparison with related species in the Cleomaceae family were carried out. The genome spans 157,714 base pairs (bp) and follows the typical chloroplast structure, consisting of a large single-copy (LSC) region (87,506 bp), a small single-copy (SSC) region (18,598 bp), and two inverted repeats (IRs) (25,805 bp each). We identified a total of 129 genes, including 84 protein-coding genes, 8 ribosomal RNA (rRNA) genes, and 37 transfer RNA (tRNA) genes. Our analysis of simple sequence repeats (SSRs) and repetitive elements revealed 91 SSRs, with a high number of A/T-rich mononucleotide repeats, which are common in chloroplast genomes. We also observed forward, palindromic, and tandem repeats, which are known to play roles in genome stability and evolution. When comparing C. houtteana with its relatives, we identified several highly variable regions, including ycf1, ycf2, and trnH–psbA, marking them as propitious molecular markers for the identification of species as well as phylogenetic studies. We examined the inverted repeat (IR) boundaries and found minor shifts in comparison to the other species, particularly in the ycf1 gene region, which is a known hotspot for evolutionary changes. Additionally, our analysis of selective pressures (Ka/Ks ratios) showed that most genes are under strong purifying selection, preserving their essential functions. A sliding window analysis of nucleotide diversity (Pi) identified several regions with high variability, such as trnH–psbA, ycf1, ndhI-ndhG, and trnL-ndhF, highlighting their potential for use in evolutionary and population studies. Finally, our phylogenetic analysis, using complete chloroplast genomes from species within Cleomaceae, Brassicaceae, and Capparaceae, confirmed that C. houtteana belongs within the Cleomaceae family. It showed a close evolutionary relationship with Tarenaya hassleriana and Sieruela rutidosperma, supporting previous taxonomic classifications. The findings from the current research offer invaluable insights regarding genomic structure, evolutionary adaptations, and phylogenetic relationships of C. houtteana, providing a foundation for future research on species evolution, taxonomy, and conservation within the Cleomaceae family. Full article

Bayanaul State National Nature Park (BSNNP), which was established in 1985 and is one of the biggest natural parks in the Republic of Kazakhstan, conserves and rehabilitates the natural flora and fauna of the Bayanaul mountain range. This article expands the floristic inventory of BSNNP and identifies the ecological and ethnobotanical importance of the park. The literature revealed that 681 plant species inhabited the BSNNP region but it was hypothesized that the park’s plant diversity was greater than the documented 681 plant species. Following our expedition travels to BSNNP, we extended the flora summary with an addition of 81 new plant species. Now, according to this study, the flora of BSNNP comprises 762 plant species belonging to 335 genera and 81 families. The leading families are Asteraceae Dumort., Poaceae Barnhart, Brassicaceae Burnett, Fabaceae Lindl, Rosaceae Juss., Caryophyllaceae Juss, Lamiaceae Lindl., Apiaceae Lindl., and Scrophulariaceae Juss. They comprise 57.7% of the total plant species in the national park and 58.5% of the total genera. The largest genera are wormwood, sedge, onion, cinquefoil, speedwell, and astragalus, based on which these genera can be considered polymorphic. Moreover, 16 species of endemic plants belonging to 14 genera and 7 families were also reported. The flora is characterized by high biological diversity with the participation of boreal relicts. The largest group among useful species is medicinal plants, represented by 186 species (24.4%) belonging to 83 genera, and 39 families. Our findings enhance the scientific understanding of plant diversity in BSNNP and provide the groundwork for future conservation research. Full article

“Banlangen” (Isatis indigotica Fort., indigowoad root) is the dried root of a plant in the Cruciferae (Brassicaceae) family, which has been cultivated in China for over 2000 years. This herb has significant medicinal value and played an important therapeutic role during the COVID-19 pandemic. Isatis indigotica is widely cultivated in China, with varying seed quality across different regions. Investigating the seed quality and distribution of endogenous metabolites of Isatis indigotica from various regions is of great significance for its efficient utilization. In this study, 21 samples from Isatis indigotica seeds were collected from 15 different regions of China and analyzed using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). A systematic analysis was conducted on seed phenotypes, germination rate, endogenous metabolites, and hormones. The results showed that the germplasm traits of Isatis indigotica from northern China were generally superior to those from southern China, with seed size positively correlated with the accumulation of sugars (D-allose, D-(+)-cellobiose, maltitol, α-D-glucopyranoside, d-galactose, maltose, D-(−)-fructose, and galactitol) and organic acids (hexadecanoic acid, benzoic acid, propanedioic acid, butanedioic acid, and cinnamic acid). Additionally, seed germination in the samples from SuQian and Shanxi was closely related to hormone distribution (gibberellic acid and abscisic acid). This study provides valuable data to support the evaluation of seeds from different sources of medicinal plants and has important implications for the cultivation and identification of Isatis indigotica varieties. Full article

Long-term opioid therapies are severely limited by the development of analgesic tolerance and gastrointestinal side effects. Camelina sativa, a plant of the Brassicaceae family, modulates the activity of peroxisome proliferator-activated receptor α (PPAR-α receptor), which is involved in the regulation of pain processing and gut physiology. The aim of this study was to evaluate the efficacy of Camelina sativa defatted seed meal (DSM) supplementation on the development of analgesic tolerance and side effects after repeated treatment with morphine in naïve mice. Co-administering Camelina sativa DSM (1 g kg⁻¹ p.o.) and morphine (10 mg kg⁻¹ s.c.) increased the efficacy and duration of the opioid-induced acute analgesic effect. Camelina supplementation also delayed the onset of tolerance to the morphine analgesic effect. The same result was obtained through either simultaneously administering morphine and camelina or administering camelina 24 h before morphine injection for the entire duration of the experiment. Camelina also counteracted intestinal damage and visceral hypersensitivity caused by morphine treatment. The beneficial effects of camelina on morphine-related analgesic efficacy and gut side effects were prevented via pre-treatment with the PPAR-α antagonist GW6471, though the latter did not influence the development of morphine tolerance. In conclusion, Camelina sativa DSM could be used as a supplement to improve the therapeutic profile of morphine. Full article

Angiosperms are the largest group of land plants with ~375,000 species, which are classified into ~416 families and ~13,000 genera; they exhibit tremendous morphological and physiological diversities and are important members of diverse terrestrial and aquatic ecosystems. Angiosperms have attracted continuous efforts to describe and understand these diversities in a framework of interrelationships—the phylogeny, which provides strong support for angiosperm classifications and relies on morphological, anatomical, and increasing molecular markers. Today, great advances in sequencing technology have led to the generation of tens of thousands of gene sequences for individual species, facilitating angiosperm phylogenetic reconstruction with high resolution at both deep and shallow levels. In this review, we present recent insights into angiosperm phylogeny based on relatively large numbers of nuclear genes, encompassing the ordinal scale of early-divergent and backbone branches, eudicots and their major subclades, asterids and rosids, as well as monocots. We further delve into intra-order cases such as Caryophyllales (Eudicots) and Alismatales (Monocots), along with intra-family relationships for some of the largest families (e.g., Asteraceae, Orchidaceae, Fabaceae, and Poaceae) and those with economic importance (such as Brassicaceae, Solanaceae, Cucurbitaceae, and Rosaceae). Furthermore, we briefly highlight the importance of nuclear phylogeny in addressing key evolutionary questions, including the origin and divergence of angiosperms, the evolution of morphological and other characters, gene duplication and other aspects of gene family evolution. Finally, we discuss possible future trends of angiosperm phylogenomics. Full article

The aim of this paper is to evaluate, from a chemical and antibacterial point of view, sprouts from species of Fabaceae and Brassicaceae families, to establish a correlation between analyzed parameters and to test the possibility of using these functional compounds in the flour food industry. The material used was lentil sprouts (LS), pea sprouts (PS), bean sprouts (BS), and radish sprouts (RS), which were chemically analyzed by determining the content of total and individual polyphenols and macro- and microelements. The antimicrobial potential of the sprout extracts obtained was tested using the disk diffusion method on five bacterial strains: Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae, Enterococcus faecalis, and Staphylococcus aureus. In order to capitalize on the flour food industry, the sprouts were introduced in the recipes for the manufacture of some cookies, and sensory analysis was performed. The results obtained indicated that LS are the sprouts with the highest content of TPC (524.130 mg/100 g) and highest content for Ni, Cu, Zn, Fe, Mn, and Mg, while the highest values for Ca were recorded in the case of PS (256.575 ± 2.23 mg/kg), and for K in the case of the BS sample (4819.450 ± 1.1 mg/kg). The RS extract has been shown to be effective against E. faecalis (MIC-70 µL/mL), S. aureus (MIC 50 µL/mL), and PS against S. aureus (MIC 70 µL/mL). Regarding the sensory analysis of cookies, the panelists participating in the study preferred products based on BS and PS in a percentage of 2.5%. Full article

Lepidium sativum, commonly known as garden cress, is a notable traditional medicinal plant within the Brassicaceae family, widely recognized for its health benefits in the globe. This study aimed to identify the seed colour variant best suited to the climate of Ethiopia, specifically in the Ataye region, by examining various agronomic traits. We conducted a comprehensive analysis of two distinct seed colour variants (brown and light blue) following standard agronomic protocols. Results indicated that the brown seed variant outperformed the light blue variant across multiple agronomic traits, demonstrating a shorter time to 50% germination, flowering, and maturation. Statistically significant differences were observed in these parameters. Additionally, plants derived from brown seeds exhibited superior growth characteristics, including greater plant height, longer primary branch length, and a higher number of primary branches per plant compared to those from light blue seeds. Furthermore, the brown seed variant displayed increased productivity, with more pods and seeds per plant, a greater one thousand seed weight, and a higher overall yield. The only traits showing no significant differences were the number of secondary branches per plant and internode length. Overall, the brown seed variant demonstrated strong adaptability to the Ataye climatic conditions, excelling in yield per hectare as well as in the number of seeds and pods produced. Based on these findings, we recommend the cultivation of brown seed colour variants for optimal performance in Ataye region, Ethiopia. Full article

This study investigates the bioactive compound profiles of 12 wild edible plant species from the Amaranthaceae, Apiaceae, Asteraceae, Brassicaceae, Caryophyllaceae, Lamiaceae, Malvaceae, Polygonaceae, and Urticaceae families, consumed as aromatic and medicinal vegetables by local communities and forming part of the natural vegetation of Mount Ergan. The plants were collected and analyzed for their anthocyanin, organic acid, and sugar contents, using advanced liquid chromatography techniques. Statistically significant differences were observed between species for each compound analyzed, highlighting their diverse phytochemical profiles. Malva neglecta, Brassica nigra, and Taraxacum phaleratum exhibited the highest anthocyanin contents, suggesting their strong potential as natural antioxidant sources. Organic acid levels were notably elevated in Polygonum cognatum, T. phaleratum, Urtica dioica, and M. neglecta, which positions these species as promising candidates for use as natural acid regulators in food and pharmaceutical formulations. In terms of sugar content, Chenopodium album, Mentha longifolia, and T. phaleratum had the lowest levels, while M. neglecta, Cirsium arvense, P. cognatum, and Tragopogon buphthalmoides showed significantly higher concentrations, indicating potential applications in the development of natural sweeteners. This study’s findings provide valuable insights into the phytochemical diversity of these wild plant species, emphasizing their potential utility in health nutrition, pharmaceuticals, and cosmetics. This study emphasizes the significance of investigating underexploited plant species for their bioactive chemicals and illustrates their potential contribution to the development of sustainable, natural product-based solutions for diverse industrial uses. Full article