Search Results (2,430)

Forest ecosystems act as major carbon sinks, highlighting the need for the accurate estimation of aboveground biomass (AGB). The Global Ecosystem Dynamic Investigation (GEDI), a full-waveform spaceborne LiDAR system developed by NASA, provides detailed global observations of three-dimensional forest structures, playing a critical role in quantifying biomass and carbon storage. However, its performance has not yet been assessed in the Mediterranean forest ecosystems of Southern Italy. Therefore, the objectives of this study were to (i) evaluate the utility of the GEDI L4A gridded aboveground biomass density (AGBD) product in the Apulia region by comparing it with the Apulia AGBD map, and (ii) develop GEDI-derived AGBD models using multiple GEDI metrics. The results indicated that the GEDI L4A gridded product significantly underestimated AGBD, showing large discrepancies from the reference data (RMSE = 40.756 Mg/ha, bias = −30.075 Mg/ha). In contrast, GEDI-derived AGBD models using random forest (RF), geographically weighted regression (GWR), and multiscale geographically weighted regression (MGWR) demonstrated improved accuracy. Among them, the MGWR model emerged as the optimal choice for AGBD estimation, achieving the lowest RMSE (14.059 Mg/ha), near-zero bias (0.032 Mg/ha), and the highest R² (0.714). Additionally, the MGWR model consistently outperformed other models across four different plant functional types. These findings underscore the importance of local calibration for GEDI data and demonstrate the capability of the MGWR model to capture scale-dependent relationships in heterogeneous landscapes. Overall, this research highlights the potential of the GEDI to estimate AGBD in the Apulia region and its contribution to enhanced forest management strategies. Full article

Adipose tissue development plays a critical role in determining carcass quality and meat production efficiency in swine; however, the regulatory mechanisms governing fat deposition remain incompletely understood. Circular RNAs (circRNAs), characterized by high stability and resistance to RNase R degradation, have emerged as important epigenetic regulators of livestock traits. This study investigated the regulatory role of circIDH2 in adipogenic differentiation of porcine preadipocytes and the underlying molecular mechanisms. Functional assays revealed that silencing circIDH2 markedly promoted preadipocyte proliferation while inhibiting differentiation and lipid accumulation; conversely, circIDH2 overexpression produced the opposite effects. Mechanistically, circIDH2 acted as a molecular sponge for miR-193a-5p through complementary base pairing, thereby relieving the repression of its target gene RASGRP4, a positive regulator of adipogenesis. Furthermore, this study demonstrated that miR-193a-5p promoted proliferation but suppressed the differentiation of porcine preadipocytes, whereas RASGRP4 inhibited proliferation while promoting adipogenic differentiation. Rescue experiments further confirmed the regulatory relationship among circIDH2, miR-193a-5p, and RASGRP4. In summary, the findings indicated that circIDH2 functioned as a key regulator of adipogenesis by modulating the miR-193a-5p/RASGRP4 axis, thereby suppressing preadipocyte proliferation and promoting adipogenic differentiation. These results provide a theoretical foundation for future investigations into the regulatory mechanisms of adipose tissue development. Full article

Nowadays, members of the genus Enterobacter have been documented as human and aquaculture pathogens. To date, no reports have described Enterobacter bugandensis infecting Hippocampus abdominalis. In the present study, an isolate of E. bugandensis, designated H4, was identified as a causative pathogen in cultured H. abdominalis following Koch’s postulate, and its virulence properties were further described. The isolate’s genome consisted of a single circular chromosome and harbored several virulence and resistance genes, including, but not limited to, csgG, acrB, hcp, gndA, galF, rpoS, fur, rcsB, and phoP involved in adherence, antimicrobial activity, effector delivery systems, immune modulation, and regulation, as well as baeR, blaACT-49, ramA, hns, ftsI, acrA, gyrA, fabI, crp, oqxB, parE, gyrB, phoP, rpoB, tuf, ptsI, and fosA2 functioning against aminoglycoside, cephamycin, disinfecting agent and antiseptic, fluoroquinolone, macrolide, peptide, and other antimicrobials. Additionally, the isolate exhibited multiple resistance to cephalosporins, penicillins, and tetracyclines and demonstrated a median lethal dose (LD₅₀) of 4.47 × 10⁵ CFU/mL in H. abdominalis. To our knowledge, this is the first study to describe E. bugandensis infecting H. abdominalis. These findings highlight the zoonotic potential of E. bugandensis and underscore the need for targeted health management in seahorse farming. Full article

Global climate warming and freshwater scarcity are intensifying water stress in agricultural fields, severely constraining sustainable agricultural development. As a typical C3 perennial cash crop, tea (Camellia sinensis) is naturally suited to low-latitude regions with abundant heat and evenly distributed precipitation, and it is highly sensitive to environmental factors such as temperature and moisture. In northern hilly tea-producing areas, tea plantations often encounter multiple challenges including uneven rainfall distribution and poor soil water retention, resulting in prominent water supply–demand imbalances that critically limit stable and efficient tea production. To explore efficient water-saving irrigation strategies adapted to such ecological conditions, this study was conducted in the Yushan Tea Plantation, Rizhao City, Shandong Province, China. Based on field monitoring data across three growing seasons (spring, summer, and autumn) from 2021 to 2023, five irrigation treatments were evaluated: conventional sprinkler irrigation (CK), drip irrigation (D), micro-sprinkler irrigation (W), drip irrigation with straw mulching (SD), and micro-sprinkler irrigation with straw mulching (SW). Actual crop evapotranspiration (ET_c act) was estimated using the soil water balance method, and actual fresh tea leaf yield (FTLY) and crop water productivity (CWP) were measured. Results showed that the SW treatment significantly improved both FTLY and CWP across all three seasons, with summer FTLY in 2022 increasing by 56.58% compared to CK and maximum CWP in spring and autumn reaching 0.916 kg/m³, demonstrating excellent stability and adaptability. Among all irrigation strategies, the SW treatment also exhibited the best regression fitting and yield prediction accuracy. The regression model validated by leave-one-out cross-validation (LOOCV) for the SW treatment demonstrated strong robustness and reliability (R² = 0.734; RMSE = 208.12 kg/ha; MAE = 183.31 kg/ha). Notably, the samples with the largest prediction errors across all treatments were nearly all associated with the highest or near-highest ET_c act values, indicating that model accuracy tends to decrease under extreme evapotranspiration conditions. The results show the synergistic effect of irrigation–mulching integration on enhancing CWP in northern perennial tea systems, providing empirical evidence and theoretical support for developing efficient irrigation strategies in hilly tea-growing regions of Northern China. Full article

Rapid urbanization and climate change have exacerbated urban heat stress, underscoring the importance of research on human thermal comfort for sustainable urban development. This study analyzes the spatiotemporal variation and driving factors of apparent temperature in the Chang–Zhu–Tan urban agglomeration, China. The Humidex index, representing apparent temperature, was derived from multi-source remote sensing data (Landsat 8, MODIS) and meteorological variables (ERA5-Land reanalysis), employing atmospheric correction, random forest modeling, and path analysis. The results indicate pronounced spatiotemporal heterogeneity: apparent temperature reached its maximum in urban centers during summer (mean 52.9 °C) and its minimum in winter (mean 5.99 °C), following a decreasing gradient from urban core to periphery. Land cover emerged as a key driver, with vegetation (NDVI, r = −0.938) showing a strong negative correlation and built-up areas (NDBI, r = +0.8) a positive correlation with apparent temperature. Uniquely, in the Chang–Zhu–Tan region’s persistently high humidity, water bodies (MNDWI, r = +0.616) exhibited a positive correlation with apparent temperature, likely due to humidity-enhanced thermal perception in summer and relatively warmer water temperature in winter. Path analysis revealed that air temperature exerts the strongest direct positive influence on apparent temperature, while relative humidity and NDVI primarily act through indirect pathways. These findings provide scientific evidence to guide climate-adaptive urban planning and enhance human living conditions in humid environments. Full article

This study investigated the relationships between inspiratory performance (IP) and glenohumeral rotation in Division 1 Collegiate baseball players (D1CBP). Thirty D1CBP were recruited. The Test of Incremental Respiratory Endurance (TIRE) provides maximal inspiratory pressure (MIP), sustained maximal inspiratory pressure (SMIP), and inspiratory duration (ID). Right and left glenohumeral internal and external rotation (RGHIR, RGHER, LGHIR, and LGHER, respectively) were measured with the shoulder in 90 degrees(d) of abduction. Significant differences between position groups were observed. IP of the entire group was significantly correlated to height, weight, and negatively correlated to right total rotational motion (RTRM) (r = −0.41; p < 0.05). The IP of all pitchers was significantly negatively correlated to both RTRM and LTRM (r = −0.56 to −0.61; p < 0.05). IP of right-handed pitchers was significantly correlated negatively to RGHER (r = −0.83 to −0.93; p < 0.05). IP of left-handed pitchers was significantly correlated negatively to LGHER (r = −0.82; p = 0.04). GH motions are significantly related to the IP of D1CBP. This association may be explained by the involvement of overstretched internal rotators, which act as accessory inspiratory muscles. Full article

Lycium barbarum L. (goji berry), a traditional Chinese medicinal plant, depends heavily on nitrogen input to maintain productivity. Nitrogen application also profoundly influences rhizosphere microbial dynamics, which are critical for soil health and plant performance. This study aimed to investigate how the rhizosphere fungal community responds to nitrogen input and explore the potential role of beneficial fungi (e.g., Chaetomium) in goji berry rhizosphere enrichment. A field experiment with four nitrogen levels (0, 53.82, 67.62, and 80.73 g·N m⁻²·year⁻¹, designated as N0, N1, N2, and N3) was conducted to analyze the fungal community structure in the rhizosphere of goji berry using ITS rRNA gene amplicon sequencing. The results showed that nitrogen input significantly altered the rhizosphere fungal community composition and diversity. Redundancy analysis (RDA) and Mantel tests indicated that soil electrical conductivity, total phosphorus, available phosphorus, and nitrate nitrogen were key environmental factors driving the fungal communities’ shifts. Notably, specific fungal genera, including Chaetomium, Cladosporium, Gibberella, Fusarium, Pyxidiophora, Acaulium, and Lophotrichus, exhibited differential enrichment across nitrogen levels. In particular, Chaetomium was significantly enriched under the conventional nitrogen treatment (N2), a strain of Chaetomium sp. LC101 was successfully isolated from the goji berry rhizosphere, and its functional roles were verified via pot experiments. Inoculation with Chaetomium sp. LC101 significantly promoted goji berry growth, with the most pronounced effects observed under N0 treatments, root fresh weight, root vitality, and leaf chlorophyll content increased by up to 55.10%, 15.69%, and 43.27%, respectively, compared to non-inoculated controls. Additionally, Chaetomium sp. LC101 regulated rhizosphere nitrogen transformation by enhancing urease, nitrite reductase, and polyphenol oxidase activities while inhibiting nitrate reductase activity. These findings demonstrate that Chaetomium responds sensitively to nitrogen input, with enrichment under moderate nitrogen levels, and acts as a beneficial rhizosphere fungus by promoting plant growth and regulating nitrogen cycling. This study provides novel insights for nitrogen management in the goji berry industry, where synergistic regulation via “nitrogen reduction combined with microbial inoculation” can reduce nitrogen loss, improve yield and quality through functional fungi, and contribute to ecological sustainability. Full article

Background: Small airway dysfunction (SAD) plays a critical role in the management of severe asthma, particularly in patients at risk of requiring biological therapies (BTs). Short-term studies have shown that switching to single-inhaler triple therapy (SITT) with extrafine beclomethasone–formoterol–glycopyrronium improves outcomes and helps achieve quiet asthma, a state marked by symptom control, no exacerbations or oral steroids, reduced inflammation, and better small airway function. This study investigated whether, over one year, patients could maintain this state as Steady Quiet Asthma (SQA) and whether baseline measures could predict this sustained response. Methods: Twenty-six patients with severe asthma and SAD were transitioned from open triple-inhaler therapy to a closed, single-inhaler triple therapy containing extrafine beclomethasone–formoterol–glycopyrronium. Assessments at baseline (T0) and at one-year follow-up (T12) included clinical evaluations, spirometry, and impulse oscillometry, with a focus on Fres as a predictor for the need for BT. When prescribed, biologic therapies included mepolizumab, benralizumab, and dupilumab. Results: Of the 26 patients, 9 (34.6%) achieved SQA and did not require biologic therapy at the one-year follow-up, while 17 patients (65.4%) initiated biologic treatment. At T0, patients who required biologics had significantly higher median Fres (21 (19.47; 24.58) vs. 17.61 (15.82; 20.63); p = 0.049) compared to those who remained biologic-free. They also exhibited higher residual volume to total lung capacity ratio (%RV/TLC) values and lower forced expiratory volume in one second/forced vital capacity ratios (FEV₁/FVC). At T12, patients spared from BT showed significant reductions in Fres (p = 0.014) and improvements in small airway function (difference in airway resistance between 5 Hz and 20 Hz (R5–20), forced expiratory flow between 25% and 75% of FVC (%FEF25–75), and better asthma control (ACT). In contrast, patients on BT demonstrated less favorable changes in these parameters. Conclusions: Baseline Fres, FEV1/FVC ratio, and %FEV25–75 are valuable predictors of achieving Steady Quiet Asthma (SQA) and sparing biologic therapy. These findings support the use of SITT in severe asthma and highlight the importance of early functional assessments to guide personalized management. Full article

Mycoplasmal pneumonia of sheep (MPS), caused by Mesomycoplasma (Mycoplasma) ovipneumoniae, profoundly impacts ovine productivity and survival. Although gut–lung microbiota interactions are increasingly recognized in respiratory diseases, whether similar crosstalk occurs between the lung and rumen microbiota in MPS-affected sheep remains unknown. To investigate alterations in the lung and rumen microbiota of sheep with MPS, the crosstalk between these microbial communities, and their impacts on growth phenotypes. From a cohort of 414 naturally infected six-month-old male Hu sheep, we selected 10 individuals with severe pulmonary pathology and 10 healthy controls for detailed phenotypic and microbiome analyses. Assessment of 359 phenotypic traits revealed that MPS significantly impairs feed efficiency and growth rate (p < 0.05). Through 16S rRNA gene sequencing, we found that MPS significantly altered the pulmonary microbiota community structure (p < 0.01), with a noticeable impact on the rumen microbiota composition (p = 0.059). Succinivibrionaceae_UCG-001 was significantly depleted in both the rumen and lungs of diseased sheep (p < 0.05) and strongly associated with reduced average daily feed intake (p < 0.05). In addition, pulmonary Pasteurella and ruminal Succinivibrionaceae_UCG-002 were significantly enriched in MPS-affected sheep, showed a strong positive correlation (p < 0.05), and were both negatively associated with feed efficiency (p < 0.05). Notably, Pasteurella multocida subsp. gallicida may act as a keystone species influencing feed efficiency. These findings point to a previously unrecognized rumen-lung microbial axis that may modulate host productivity in sheep affected by MPS. This work provides new insights into the pathogenesis of MPS and offers potential targets for therapeutic intervention and management. Full article

The amygdala is composed of several nuclei, including the lateral nucleus which is the main receiving area for the input from cortical and subcortical brain regions. It mediates fear, anxiety, stress, and pain across species. Evidence suggests that the endocannabinoid system may be a promising target for modulating these processes. Cannabinoid and cannabinoid-related receptors have been identified in the amygdala of rodents, carnivores, and humans, but not in horses. This study aimed to investigate the gene expression of cannabinoid receptors 1 (CB1R) and 2 (CB2R), transient receptor potential vanilloid 1 (TRPV1), and peroxisome proliferator-activated receptor gamma (PPARγ) within the lateral nucleus of six equine amygdalae collected post mortem from an abattoir using quantitative real-time PCR, cellular distribution, and immunofluorescence. mRNA expression of CB1R and CB2R, but not TRPV1 or PPARγ, was detected. The percentage of immunoreactivity (IR) was calculated using ImageJ software. Cannabinoid receptor 1 immunoreactivity was absent in the somata but was strongly detected in the surrounding neuropil and varicosities and CB2R-IR was observed in the varicosities; TRPV1-IR showed moderate expression in the cytoplasm of somata and processes, while PPARγ-IR was weak-to-moderate in the neuronal nuclei. These findings demonstrate endocannabinoid system components in the equine amygdala and may support future studies on Cannabis spp. molecules acting on these receptors. Full article

Protein enzymes are highly efficient catalysts that exhibit adaptability and selectivity under diverse biological conditions. In some organisms, such as bacteria, structurally similar enzymes, for instance, shikimate kinase (SK) and adenylate kinase (AK), coexist and act on chemically related ligands. This raises the question of whether these enzymes can accommodate and potentially react with each other’s ligands. In this study, we investigate the stability of non-cognate ligand binding in SK and explore whether external electric fields (EFs) can modulate this interaction, leading to cross-reactivity in SK. Using molecular dynamics simulations, we assess the structural integrity of SK and the binding behavior of ATP and AMP under EF-off and EF-on cases. Our results show that EFs enhance protein structure stability, stabilize non-cognate ligands in the binding pocket, and reduce local energetic frustration near the R116 residue located in the binding site. In addition to this, dimensionality reduction analyses reveal that EFs induce more coherent protein motions and reduce the number of metastable states. Together, these findings suggest that external EFs can reshape enzyme–ligand interactions and may serve as a tool to modulate enzymatic specificity and functional promiscuity. Thus, we provide computational evidence that supports the concept of using an EF as a tunable parameter in enzyme engineering and synthetic biology. However, further experimental investigation would be valuable to assess the reliability of our computational predictions. Full article

Cardiopulmonary bypass (CPB) is associated with significant neurological complications, yet the mechanisms underlying brain injury remain unclear. This study investigated the role of interleukin-17A (IL-17A) in exacerbating CPB-induced neuronal apoptosis and identified vulnerable brain regions. Utilizing a rat CPB model and an oxygen–glucose deprivation/reoxygenation (OGD/R) cellular model, we demonstrated that IL-17A levels were markedly elevated in the hippocampus post-CPB, correlating with endoplasmic reticulum stress (ERS)-mediated apoptosis. Transcriptomic analysis revealed the enrichment of IL-17 signaling and apoptosis-related pathways. IL-17A-Neutralizing monoclonal antibody (mAb) and the ERS inhibitor 4-phenylbutyric acid (4-PBA) significantly attenuated neurological deficits and hippocampal neuronal damage. Mechanistically, IL-17A activated the Act1-IRE1-JNK1 axis, wherein heat shock protein 90 (Hsp90) competitively regulated Act1-IRE1 interactions. Co-immunoprecipitation confirmed the enhanced Hsp90-Act1 binding post-CPB, promoting IRE1 phosphorylation and downstream caspase-12 activation. In vitro, IL-17A exacerbated OGD/R-induced apoptosis via IRE1-JNK1 signaling, reversible by IRE1 inhibition. These findings identify the hippocampus as a key vulnerable region and delineate a novel IL-17A/Act1-IRE1-JNK1 pathway driving ERS-dependent apoptosis. Targeting IL-17A or Hsp90-mediated chaperone switching represents a promising therapeutic strategy for CPB-associated neuroprotection. This study provides critical insights into the molecular crosstalk between systemic inflammation and neuronal stress responses during cardiac surgery. Full article

The paper studies the behavior of homogeneous isotropic materials by performing appropriate numerical analyses and utilizing suitable FEMs to reproduce the Brazilian splitting test. Starting with a theoretical approach and adopting suitable numerical simulations, a new formula that is able to characterize the Young’s modulus is presented. To this end, in addition to the analysis of the specimen’s response in terms of stresses and strains, the real displacement field resulting from the real kinematical constraints on the specimen is determined. Therefore, the Brazilian test is taken as a reference test and the specimen’s behavior is derived by taking advantage of both the theoretical approach and numerical simulations developed in the ANSYS 2021 R1 environment. The latter allows us to define a new mathematical relation representing the missing part of the kinematical field. Furthermore, a new formula which explicitly relates the Young’s modulus of the material to the geometrical characteristics of the specimen, to the acting force, and to a measured selected displacement is proposed. Future developments will include adopting the proposed formulas for the identification of other mechanical parameters of the material, e.g., by adopting a full-field contactless approach to displacement measurement and studying the behavior of specimens with different geometrical characteristics. Full article

Gestational diabetes mellitus (GDM) and iron deficiency anemia (IDA) are the most common pregnancy-related conditions resulting in adverse maternal and fetal complications. MicroRNAs (miRNAs), particularly miR-182-3p and miR-24-3p, are promising biomarkers as they act as regulatory elements in various diseases; however, their roles in GDM and IDA are unclear. The present study aimed to analyze the expression and functional relevance of miR-182-3p and miR-24-3p in GDM and IDA. Experimental validation via RT-PCR revealed significant upregulation of both miRNAs in GDM and IDA samples. We identified common target genes and signaling pathways associated with these miRNAs, using a combination of data mining, bioinformatic tools (miRDB, TargetScan, miRTarBase, and miRWalk), and differentially expressed gene (DEGs) analysis using the GEO, OMIM, MalaCards, and GeneCards datasets. GO and KEGG pathway analyses revealed that the shared miRNA–mRNA in target genes were enriched in insulin signaling, apoptosis, and inflammatory pathways—key mechanisms implicated in GDM and IDA. Furthermore, hub genes such as IRS1, PIK3CA, CASP3, MAPK7, and PDGFRB were identified, supporting their central role in metabolic dysregulation during pregnancy. These findings demonstrate the potential of miR-182-3p and miR-24-3p as diagnostic biomarkers and therapeutic targets in managing GDM and IDA, offering new insights into the molecular interplay underlying pregnancy complications. Full article

Insects and their bacterial endosymbionts form intricate ecological relationships, yet their role in host–pathogen interactions are not fully elucidated. The small brown planthopper (Laodelphax striatellus), a polyphagous pest of cereal crops, acts as a key vector for rice stripe virus (RSV), a significant threat to rice production. This study aimed to compare the endosymbiont community structures of nonviruliferous and RSV-viruliferous L. striatellus populations using 16S rRNA gene sequencing with high-throughput sequencing technology. Wolbachia was highly dominant in both groups; however, the prevalence of other endosymbionts, specifically Rickettsia and Burkholderia, differed markedly depending on RSV infection. Comprehensive microbial diversity and composition analyses revealed distinct community structures between nonviruliferous and RSV-viruliferous populations, highlighting potential interactions and implications for vector competence and virus transmission dynamics. These findings contribute to understanding virus-insect-endosymbiont dynamics and could inform strategies to mitigate viral spread by targeting symbiotic bacteria. Full article