Search Results (25)

Additive manufacturing (AM) techniques have transformed the production of parts and components with intricate geometries and customized designs, driving innovation in sustainable manufacturing practices. The additive manufacturing technology used in this work was selective laser melting (SLM), a process that uses laser energy to sinter powdered metals into solid structures. Among the various materials utilized in AM, Ti6Al4V titanium alloys are of particular interest due to their favorable mechanical properties, corrosion resistance, biocompatibility, and potential for reducing material waste. However, the machining of additively manufactured titanium parts presents challenges due to the material’s low conductivity, elastic modulus, and chemical affinity with cutting tools, which impact tool wear and surface finish quality. Milling, a commonly employed process for finishing titanium parts, often involves significant energy use and tool wear, highlighting the need for optimized and eco-conscious machining strategies. This study aims to establish correlations among four key aspects: (1) surface finish of machined Ti6Al4V AM parts, (2) cutting tool damage, (3) dry milling parameters including different cutting tools, and (4) variation of temperature at the contact surface of AM parts and tools using infrared thermography. By examining parameters such as feed per tooth (Fz), axial depth of cut (Ap), spindle trajectories (trochoidal, helicoidal, and linear), and cutting tool diameters, this work identifies conditions that enhance process efficiency while reducing environmental impact. Infrared thermography provides insights into temperature variations during milling, correlating these changes to surface roughness and critical machining parameters, thus contributing to the development of sustainable and high-performance manufacturing practices. Full article

As a result of the continuous use of persistent per- and polyfluoroalkyl substances (PFAS), e.g., in aviation firefighting foams, contamination with PFAS has been found in soil, groundwater, and surface water around thousands of industrial and military installations. Due to their harmful (environmental) potential, further dispersion in the environment needs to be stopped, which can be achieved by appropriate absorption materials. In this work, the influence of the cross-linking agent epichlorohydrin (ECH) concentration on the perfluorooctanesulfonic acid (PFOS) adsorption capacity of chitosan gel was investigated. It was found that higher ECH concentration during the cross-linking step decreases the PFOS adsorption capacity of the cross-linked chitosan gel from 0% to 4% ECH solution by about 15%. Using a concentration of 1%, ECH resulted still in an acid-stable material, and a maximum PFOS loading capacity of 4.04 mmol/g was obtained, one of the highest described in the literature. Furthermore, we used a rapid small-scale column test to compare the PFOS adsorption capacity of chitosan and activated carbon, each in both milled and unmilled form. Unmilled chitosan showed the highest PFOS adsorption capacity considering adsorption material dry masses (>0.9 and <0.4 mmol/g for both types of chitosan and activated carbon, respectively). Milled activated carbon proved to be the better adsorption material, considering the fixed volume of the adsorber (>99.9% PFOS adsorbed). Overall, the cross-linking agent concentration in chitosan is a crucial factor influencing its PFOS absorption potential. Our results feature cross-linked chitosan as an effective economic and ecologic alternative for PFOS adsorption in aqueous solutions. Full article

In this study, two by-products resulting from the processing of cherry (stems and pits) were used as natural coagulants to promote the valorization of these wastes and treat olive mill wastewater (OMW). The efficacy of the plant-based coagulants (PBCs) in the coagulation–flocculation–decantation process (CFD) was evaluated through the removal of turbidity, total suspended solids (TSS), total polyphenols (TPh), and dissolved organic carbon (DOC). The CFD process was demonstrated to be effective in turbidity and TSS reduction in OMW. Using cherry stems (CSs), these reductions were 65.2% of turbidity and 58.0% of TSS, while cherry pits (CPs) achieved higher reductions, 78.6% of turbidity and 68.2% of TSS. To improve the effectiveness of OMW treatment, mainly regarding the removal of TPh and DOC, the CFD process was complemented with the adsorption process (using bentonite clay). The adsorption capacity of bentonite was higher in acidic conditions (pH 3.0) and, with a dosage of 3.0 g L⁻¹, reached 17.3 mg of DOC and 13.8 mg of TPh per gram of bentonite. Several adsorption isothermal models were assessed, and the Langmuir (r² = 0.985), SIPS (r² = 0.992), and Jovanovic models (r² = 0.994) provided the best fittings. According to the optimal operational conditions defined throughout the present work, the combination of CFD and adsorption removals were as follows: (1) 98.0 and 91.3% of turbidity, (2) 80.8 and 81.2% of TSS, (3) 98.1 and 97.6% of TPh and (4) 57.9 and 62.2% of DOC, for CSs and CPs, correspondingly. Overall, the results suggest that cherry by-products can be used as low-cost natural coagulants and, when combined with another natural, abundant, and cheap material, such as bentonite clay, can be a sustainable alternative for treating OMW. Full article

The rotor or impeller is a rotational and key part of a pump and compressor. This article presents the detailed development process of a rotor of small size constructed from an EN8 steel cylindrical blank using a novel technique based on a computer numerical control engraving milling machine (CNC-EMM) equipped with a 4 mm tungsten carbide end mill cutter. We fabricated a total of twenty-eight stepped rotors following the Box–Behnken Design (BBD) DoE technique at fourteen distinct combinations of CNC-EMM variable parameters, namely rotational speed, feed, and plunge feed. Average roughness ‘R_a’, an important surface quality indicator, has been considered and presented in this article, as a quality measure for the fabricated rotors. Feed and plunge feed have been identified as the most influencing variable parameters as per an analysis of variance (ANOVA) test. The lowest average roughness value obtained by this process for the rotor blade was 0.11 µm. A micrograph obtained from a field-emission scanning electron microscope (FE-SEM) showed a uniform and accurate tooth profile along with burr formation at corner edges. This study claims to establish engraving milling as a viable alternative to other manufacturing processes used for rotor blades. The findings of this study are useful to scholars, engineers, and researchers who are exploring new ways to fabricate mechanical parts and components. Full article

Dioscorea spp. is known for its myriad medicinal properties. D. alata, specifically crude extracts, have displayed potent anticancer properties. However, the chemical constituents of these extracts have not been examined. The aim of this study is to determine the chemical composition and antioxidant characteristics of the active extracts from D. alata tuber. Chemoinformatic profiling of the Jamaican Dioscorea alata cultivar white yam tuber was generated by a sequential Soxhlet extraction of dried milled tuber, producing five crude extracts: hexane (E-1), diethyl ether (E-2), acetone (E-3), ethanol (E-4) and water (E-5). The analytes within the five extracts were dissolved in 0.1% DMSO and their anticancer activity was determined using DU145 prostate cancer cells. Both the acetone and the ethanolic extract were able to induce greater than 50% cell death at 50 µg/mL. The order of growth inhibition of the extracts in DU-145 cell is E3 (IC₅₀, 10.81 µg/mL) > E-4 (IC₅₀ 24.17 µg/mL) > E-1 (IC₅₀ > 100 µg/mL) ≥ E-2 (IC₅₀ > 100 µg/mL) ≥ E-5 (IC₅₀ > 100 µg/mL). Phytochemical screening of both E-3 and E-4 revealed the presence of all major classes of secondary metabolites except tannins. Resins were also absent in the E-3 extract. Phenolic quantification indicated that E-3 and E-4 possessed GAEs of 31 ± 1.1 and 72 ± 1.8 mg per g of sample, respectively. Inversely, E-3 displayed greater antioxidant capability with IC₅₀ of 82.9 µg/mL compared to E-4 (166.9 µg/mL); however, neither was comparable to citric acid (33.6 µg/mL). The extract E-3 was further isolated by HPLC into 11 fractions. Fractions 4 and 5 possessed potent cell growth inhibitory effects. GCMs of fractions 4 and 5 showed they possessed numerous saturated fatty acids with pharmacological relevance. The presence of these compounds shows potential for exploitation of D. alata extracts for pharmacological purposes. Full article

Rice self-sufficiency is central to Indonesia’s agricultural development, but the country is increasingly challenged by population growth, climate change, and arable land scarcity. Agroecological nutrient management offers solutions though optimized fertilization, enhanced organic matter and biofertilizer utilizations, and improved farming systems and water management. Besides providing enough nutrients for crops, the agroecological approach also enhances resilience to climate change, reduces the intensity of greenhouse gas emissions, and improves the biological functions of rice soil. Organic and bio fertilizers can reduce the need for chemical fertilizers. For example, blue-green algae may contribute 30–40 kg N ha⁻¹, while the application of phosphate solubilizing microbes can reduce the use of chemical phosphorous fertilizers by up to 50 percent. The country currently experiences substantial yield gaps of about 37 percent in irrigated and 48 percent in rain-fed rice. Achieving self-sufficiency requires that Indonesia accelerates annual yield growth through agroecological nutrient management from a historical 40 kg ha⁻¹ year⁻¹ to 74 kg ha⁻¹ year⁻¹. The aim is to raise the average yield from the current 5.2 t ha⁻¹ year⁻¹ to 7.3 t ha⁻¹ year⁻¹ by 2050. Simultaneously, controlling paddy field conversion to a maximum of 30,000 hectares per year is crucial. This strategic approach anticipates Indonesia’s milled rice production to reach around 40 million metric tonnes (Mt) by 2050, with an expected surplus of about 4 Mt. Full article

This study investigates the chemical composition of soyhulls (SHs) as an alternative feed ingredient and their effect on nutrient and amino acid (AA) digestibility in laying hens during peak production. A total of 200 golden brown hens (28 weeks old) were subjected to random allocation across 5 dietary treatments: a corn–soybean meal (SBM) reference diet and 4 test diets with 25% SHs from different mills (SH1, SH2, SH3, and SH4). Each treatment was replicated four times with ten birds per replicate. Digesta samples were collected during three phases (28–32, 32–36, and 36–40 weeks of age) to measure apparent metabolizable energy (AME), the apparent ileal digestibility (AID) of nutrients, and the standard ileal digestibility (SID) of AAs. The SBM diet had 30.0% crude protein (CP) and 3.78% crude fiber (CF), while the SH diets had 21.0 to 21.5% CP and 11.6% CF. The findings revealed that the AME was lower (p < 0.05) with SH diets (2404 kcal/kg) compared to the SBM diet (2627 kcal/kg) in all three phases. The SH diets had a lower AID of dry matter (DM), crude protein (CP), ash, ether extract (EE), and crude fiber (CF) than the SBM diet by an average of 2.88, 2.25, 4.93, 4.99, and 3.36%, respectively. The AID of nitrogen-free extract (NFE) was higher in the SH diets than the SBM diet by 3.42% in all three phases (p < 0.05). The SH diets had lower uric acid excretion (about 66.93 mg/100 mL) than the SBM diet (about 76.43 mg/100 mL) on average in all three phases. The SH diets had a lower SID of arginine, histidine, isoleucine, lysine, cysteine, valine, and tyrosine than the SBM diet by 2 to 10%, while the SID of methionine was higher in the SH diets than the SBM diet by 2.2% on average in all three phases (p < 0.05). The SH from Sadiq Brother Feed (SH1) had the highest AME and AID of DM, ash, CP, EE, CF, and the SID of AA among the SH diets. These results indicate that SH can partially replace SBM in laying hen diets, but the source and quality of SH should be considered. Full article

This paper details an experimental investigation on the influence of the size effect when slot-milling a CMSX-4 single-crystal nickel-based superalloy using 1 mm- and 4 mm-diameter TiAlN-coated tungsten carbide (WC) end-mills. With all tools having similar cutting-edge radii (r_e) of ~6 µm, the feed rate was varied between 25–250 mm/min while the cutting speed and axial depth of cut were kept constant at 126 m/min and 100 µm, respectively. Tests involving the Ø 4 mm end-mills exhibited a considerable elevation in specific cutting forces exceeding 500 GPa, as well as irregular chip morphology and a significant increase in burr size, when operating at the lowest feed rate of 25 mm/min. Correspondingly for the Ø 1 mm micro-end-mills, high levels of specific cutting forces up to ~1000 GPa together with severe material ploughing and grooving at the base of the machined slots were observed. This suggests the prevalence of the size effect in the chip formation mechanism as feed per tooth/uncut chip thickness decreases. The minimum uncut chip thickness (h_min) when micromilling was subsequently estimated to be less than 0.10 r_e, while this increased to between 0.10–0.42 r_e when machining with the larger Ø 4 mm tools. Full article

In a protein reduction feeding trial (Study 1) on a commercial broiler farm in northern Germany, it was attempted to be shown that research results from station tests on protein reduction can be transferred to agricultural practice. In a second study, the limits of the N reduction were tested in a research facility. In Study 1, commercial standard feeds were fed to the control group (variant 1:210,000 animals; n = 5 barns). In the test group (variant 2:210,000 animals; n = 5 barns), the weighted mean crude protein (CP) content was moderately reduced by 0.3%. The nitrogen reduction in the feed did not affect performance (feed intake (FA), daily gain (DG), feed conversion (FCR)), but nitrogen conversion rate increased from approx. 61% to approx. 63%. The solid litter weight was reduced by 12% and nitrogen excretion by 9% (p < 0.05). Significantly healthier footpads were due to lower water intake (−4%; p < 0.05) and a numerically drier bedding. In Study 2, responses of treatments (1250 broiler per variant; n = 5) showed that sharper N-lowering (−1.5% CP; weighted average) did not impair performance either, but N-conversion improved and N-excretions decreased significantly. Converted to a protein reduction of one percentage point, the N excretions were able to be reduced by 22% in Study 1 and 18% in Study 2. Feeding trials in the commercial sector, such as the present Study 1, should convince feed mills and farmers to allow the latest scientific results to be used directly and comprehensively in commercial ration design. Full article

A well-made provisional fixed prosthesis must present as a preview of the future prosthesis and may also augment the health of the abutments and periodontium. Provisional restorations have been prepared chairside with polymethyl methacrylate (PMMA) since time immemorial. CAD/CAM additive and subtractive technologies have revolutionized the fabrication of interim restorations in dental clinics. The current literature lacks substantial data about retention of provisional crowns manufactured using Computer-Aided Design/Computer-Aided Manufacturing (CAD/CAM) additive and subtractive techniques with various temporary cements. This in vitro study aims to assess and compare the retention of temporary/provisional anterior crowns based on the combined effect of different digital manufacturing techniques, preparation tapers, and the temporary cements used for cementation. Two maxillary right central incisor typodont teeth were prepared to receive all-ceramic crowns, one with a 10-degree taper and the other with a 20-degree taper. Forty 3D-printed working models with the 10° taper and forty working models with the 20° taper were prepared to receive the temporary crowns. Forty temporary crowns were 3D-printed and forty crowns were milled (20 from each taper group). Kerr Temp-Bond NE conventional cement and Kerr Temp-Bond clear cement were used for cementation in the two groups. The number of samples per test group was 10. All samples were thermocycled and subjected to a universal testing machine to measure the pull-off force until retention loss (N) under tension with a crosshead speed of 5 mm/min. The pull-off force was highest for group 8, i.e., 3D-printed crowns with a 20° taper and cemented with Kerr Temp-Bond clear cement, followed by groups 6, 7, 4, 5, 3, and 2. Group 1, i.e., milled crowns with 10° taper cemented with Kerr Temp-Bond NE conventional cement, exhibited the lowest pull-off retentive force. The clinical selection of long-term provisional crowns fabricated using 3D-printing technology, prepared with 10° or 20° tapers, and cemented with clear cement, is the most favorable in terms of the retention of provisional crowns. 3D-printed provisional crowns can be used as an alternative to conventional and CAD/CAM-milled crowns for long-term provisionalization. Full article

Newly released sugarcane varieties need to be adapted to various environments. This research was aimed at examining the growth and yield potential of newly released varieties of sugarcane in the first year as plant cane (PC) and the second year as first ratoon cane (RC1) on dry land. The research was carried out at Wedarijaksa station, Trangkil Sugar Mill area, Pati, Central Java in 2019–2021. Four sugarcane varieties were grown using a double rows system, AAS Agribun, ASA Agribun, AMS Agribun, and CMG Agribun and one commercial variety, PSJK 922. Measurements of crop growth were made periodically: yield components at harvest in PC-RC1, and physiological characteristics 5 months after planting. The results indicate that mean tonnes of cane and sugar per hectare between PC and RC1 decreased by 22.7% and 21.0%, respectively, for AAS Agribun, ASA Agribun, and CMG Agribun due to decreased stem weights. AMS Agribun showed the smallest decrease in tonnes of cane (4%) and increase in tonnes of sugar per hectare (2%) from PC to RC1. The highest number of tonnes of sugar in PC was achieved by ASA Agribun (12.8 t ha⁻¹), slightly above PSJK 922 (12.69 t ha⁻¹). The decline in tonnes of cane and sugar needs to be reduced by the continuously improving cultivation techniques. The mean photosynthetic water use efficiency of tested new varieties was 7.46 µmol CO₂ mol H₂O⁻¹. These research findings provide information on crop performance and can be used as a basis for selecting varieties to be developed in the region. Further studies will be required to test these new sugarcane varieties in a wide range of agroecological zones in Indonesia. Full article

An alternative in vitro propagation protocol for medical Cannabis sativa L. cultivars for pharmaceutical industrial use was established. The aim of the protocol was to reduce the culture time, offering healthy and aseptic propagating material, while making the whole process more economic for industrial use. The propagation procedure was performed using plastic autoclavable vented and non-vented vessels, containing porous rooting fine-milled sphagnum peat moss-based sponges, impregnated in ½ Murashige and Skoog liquid growth medium, supplemented with indole-3-butyric acid (IBA) at various concentrations (0, 2.46, 4.92, and 9.84 µM) or by dipping nodal cuttings into 15 mM IBA aqueous solution. The highest average root numbers per cutting, 9.47 and 7.79 for high cannabidiol (H_CBD) and high cannabigerol (H_CBG) varieties, respectively, were achieved by dipping the cuttings into IBA aqueous solution for 4 min and then placing them in non-vented vessels. The maximum average root length in H_CBD (1.54 cm) and H_CBG (0.88 cm) was ascertained using 2.46 μM filter sterilized IBA in non-vented vessels. Filter-sterilized IBA at concentrations of 2.46 μM in vented and 4.92 μM in non-vented vessels displayed the maximum average rooting percentages in H_CBD (100%) and H_CBG (95.83%), respectively. In both varieties, maximum growth was obtained in non-vented vessels, when the medium was supplemented with 4.92 μM filter-sterilized IBA. Significant interactions between variety and vessel type and variety and IBA treatments were observed in relation to rooting traits. Approximately 95% of plantlets were successfully established and acclimatized in field. This culture system can be used not only for propagating plant material at an industrial scale but also to enhance the preservation and conservation of Cannabis genetic material. Full article

The aim of this study was to investigate whether the polymer polyether ether ketone (PEEK), which is approved for (dental) medical appliances, is suitable for the production of orthodontic treatment appliances. Different geometries of transpalatal arches (TPAs) were designed by Computer Aided Design (CAD). Out of a number of different designs and dimensions, four devices were selected and manufactured by milling out of PEEK. A finite element analysis (FEA) and a mechanical in vitro testing were performed to analyze the force systems acting on the first upper molars. Up to an activation (transversal compression) of 4 mm per side (total 8 mm), the PEEK TPAs generated forces between 1.3 and 3.1 Newton (N) in the FEA and between 0.7 and 3.2 N in the mechanical testing. The moments in the oro-vestibular direction were measured between 2.1 and 6.6 Nmm in the FEA and between 1.1 and 6.0 Nmm in the mechanical testing, depending on the individual TPA geometry. With the help of the FEA, it was possible to calculate the von Mises stresses and the deformation patterns of the different TPAs. In some areas, local von Mises stresses exceeded 154–165 MPa, which could lead to a permanent deformation of the respective appliances. In the in vitro testing, however, none of the TPAs showed any visible deformation or fractures. With the help of the FEA and the mechanical testing, it could be shown that PEEK might be suitable as a material for the production of orthodontic TPAs. Full article

Integrating leys, cover crops, and animal manures constitute promising avenues to reach annual soil organic carbon changes (ΔSOC) >0.4% in forage and grain-based crop rotations, rates required to offset the increasing C emissions from fossil fuels (“4 per mille” initiative). How these practices and rotations perform in reaching this aim was object of analysis in this paper. Five cropping systems (CS), including three three-year forage and grain-based crop rotations containing annual grass-clover leys (FR and MR) or cover crops (GR), and two contrasting controls (continuous silage maize (CM), and permanent grassland (PG)) were compared for their impact on SOC stocks over eight years (2010–2018). The CS were unfertilized (N0) or fertilized using cattle slurry (N1) at a rate of 240 kg N ha⁻¹ yr⁻¹ applied in the non-leguminous crops. The ΔSOC of the top 30 cm soil layer and the annual carbon inputs (C_in) from slurry applications and plant residues were estimated, their relationship established, and the slurry-induced C retention coefficient was determined. The FR and MR SOC stocks remained stable at N1, while the GR and CM SOC decreased over time by tendency even at N1. Only the PG reached ΔSOC >0.4%. Differences in ΔSOC between CS and N rates were highly associated with the system-specific increase in belowground C_in, induced by slurry applications. Slurry-induced C retention coefficients differed strongly between CS: CM (3%) followed by GR (12%), and by FR and MR (20–15%), and lastly by PG (24%). Promoting belowground carbon inputs was identified as an efficient way to reach significant increases in ΔSOC. We conclude that a ley in only one out of three years is not sufficient to significantly increase SOC stocks in arable crop rotations of the study region. Full article

Polycarbonates are polymers of bisphenol A (BPA), a well-known endocrine disruptor. This study evaluated the release of BPA from polycarbonate crowns that were (1) milled from Temp Premium Flexible (ZPF, Zirkonzahn, Italy) or Tizian Blank Polycarbonate (TBP, Schütz Dental, Germany), or (2) 3D-printed (Makrolon 2805, Covestro, Germany). Commercial prefabricated polycarbonate crowns (3M, USA) and milled poly(methyl methacrylate) (PMMA) crowns (Temp Basic, Zirkonzahn, Italy) were included for comparison. The crowns were stored at 37 °C in artificial saliva (AS) or methanol, which represented the worst-case scenario of BPA release. Extracts were collected after 1 day, 1 week, 1 month and 3 months. BPA concentrations were measured using liquid chromatography-tandem mass spectrometry. The amounts of released BPA were expressed in micrograms per gram of material (μg/g). After 1 day, the highest amounts of BPA were measured from milled polycarbonates, TBP (methanol: 32.2 ± 3.8 μg/g, AS: 7.1 ± 0.9 μg/g) and ZPF (methanol 22.8 ± 7.7 μg/g, AS: 0.3 ± 0.03 μg/g), followed by 3D-printed crowns (methanol: 11.1 ± 2.3 μg/g, AS: 0.1 ± 0.1 μg/g) and prefabricated crowns (methanol: 8.0 ± 1.6 μg/g, AS: 0.07 ± 0.02 μg/g). Between 1 week and 3 months, the average daily release of BPA in methanol and AS decreased below 2 μg/g and 0.6 μg/g, respectively. No BPA was released from PMMA in AS, and the cumulative amount released in methanol was 0.2 ± 0.06 μg/g. In conclusion, polycarbonates could be a relevant source of BPA, but the current tolerable daily intake of BPA (4 μg/kg body weight) should not be exceeded. Full article