Search Results (205)

In the coming decades, the agricultural system will predictably rely on organic material to produce crops and maintain food security. Currently, the use of inorganic fertilizers to grow crops and vegetables, such as Swiss chard, spinach, and lettuce, is on the rise and has been proven to be detrimental to the soil in the long run. Hence, there is a growing need to use organic waste material, such as spent coffee grounds (SCGs), to grow crops. Spent coffee grounds are made of depleted coffee beans that contain important soluble compounds. This study aimed to determine the influence of different levels (0.32 g, 0.63 g, 0.92 g, and 1.20 g) of spent coffee grounds on the metabolomic profile of Swiss chard. The 1H-nuclear magnetic resonance (NMR) results showed that Swiss chard grown with different levels of SCGs contains a total of 10 metabolites, which included growth-promoting metabolites (trehalose; betaine), defense mechanism metabolites (alanine; cartinine), energy-reserve metabolites (sucrose; 1,6 Anhydro-β-D-glucose), root metabolites (thymine), stress-related metabolites (2-deoxyadenosine), caffeine metabolites (1,3 Dimethylurate), and body-odor metabolites (trimethylamine). Interestingly, caprate, with the abovementioned metabolites, was detected in Swiss chard grown without the application of SCGs. The findings of the current study suggest that SCGs are an ideal organic material for growing Swiss chard for its healthy metabolites. Full article

This investigation proposes an effective protocol (cutting) for Paulownia tomentosa production in Tunisia during the 2022–2024 period. The effects of the three interactive parameters: root cutting diameter (L1, 0.5; L2, 0.8; L3, 1.25; and L4, 2 cm), indole-3-butyric acid (IBA) hormone concentrations (C, 0; T1, 0.1%; and T2, 0.3%), and soil type (S1: 50% silt + 50% potting soil, and S2: 43% potting soil + 43% silt + 14% sand), were investigated. The data showed that cutting roots with 0.5 cm dimensions, a cutting treatment with hormone (0.3%), and the S2 soil type corresponded to a growth enhancement in height. These results revealed the broad changes in flowering of P. tomentosa. Also, data revealed that the root cutting diameter had the greatest influence on the biochemical contents of 4-month-old P. tomentosa sprouts. The studied pathway revealed that the auxin precursor IBA contributes toward active auxin [indole-3-acetic acid (IAA)] biosynthesis. Overall, this study found substantial changes in the morphological, biochemical, and floral features of new P. tomentosa sprouts under the interactive factors. To summarize, vegetative propagation of Paulownia, particularly through root cutting, allows for proliferation and plantation development. These findings can be applied to future breeding efforts with Paulownia to improve and protect it as a woody species, forage, and medicinal plant. Full article

Among the numerous diseases that affect papaya (Carica papaya L.) cultivation, anthracnose, caused by a complex of fungi from the genus Colletotrichum spp., stands out, primarily due to its damage to the commercial part of the papaya, the fruit, specifically the pulp. Although chemical control with synthetic molecules is the most commonly used method to combat anthracnose, it is not the most appropriate solution. The indiscriminate use of synthetic chemical products results in numerous harmful effects on the environment, the health of farmers, and the final consumers. Given these circumstances, the objective of this study was to analyze the efficacy of essential oils (EOs) from Citrus aurantium var. dulcis L., known as sweet orange, Citrus limon (L.), known as Sicilian lemon, and the major compound present in these oils, limonene, against the pathogens Colletotrichum okinawense, which cause anthracnose in papaya fruits. The percentage inhibition of mycelial growth was evaluated on the seventh day, with estimates of 50% and 90% inhibition, to compare the inhibitory effect among the fungal isolates. Chromatographic analysis revealed that sweet orange EO contains myrcene and limonene. Sicilian lemon essential oil includes myrcene, limonene, α- and β-pinene, and γ-terpinene. Both EOs and limonene exhibited activity against C. okinawense. The 50 µL/mL concentration was the most effective in inhibiting growth. The EOs and limonene showed similar IC₅₀ values, with limonene at 48 µL/mL, Sicilian lemon EO at 51 µL/mL, and sweet orange EO at 57 µL/mL. Full article

Saline environments shape plant metabolism, driving ecological and biochemical adaptations. This study investigated the impact of salinity on Adenosma indiana (Indian scent-wort), a medicinal herb known for its volatile organic compounds (VOCs) and anti-inflammatory and antimicrobial properties, to elucidate its adaptive strategies. During the flowering stage, samples were collected from four saline microhabitats in Kalasin Province, Thailand. We analyzed soil properties, plant growth, photosynthetic pigments, compatible solutes (anthocyanins, proline, total sugars), and elemental concentrations (K, Na, Ca, Mg) across different tissues. Results showed that A. indiana maintained stable growth while enhancing chlorophyll and β-carotene levels under increasing salinity. GC-MS identified 47 VOCs, including 3-cyclopenten-1-one (first reported in this species) and β-bisabolene, both strongly linked to soil salinity. In low-salinity soils, leaves accumulated high sodium, inducing osmoprotectants (proline, total sugars) and VOCs (D-limonene, α-pinene, terpinolene, 1-octen-3-ol) in peltate glandular trichomes. Conversely, in high-salinity soils, lower leaf sodium levels were associated with increased β-bisabolene and β-caryophyllene production, suggesting distinct biochemical pathways. These findings reveal salinity-driven VOC modulation in A. indiana, highlighting its adaptive potential for medicinal applications in saline environments and its role as a source of salt-tolerant bioactive compounds. Full article

Changes in the environment have a significant impact on photosynthetic efficiency, which in turn influences plant growth and yield. Consequently, there is a greater focus on methods to enhance photosynthetic efficiency with the goal of raising plant productivity. In this study, the effects of titanium oxide nanoparticles (TiO₂ NPs) on pea plants (Pisum sativum L.) subjected to moderate salt stress by the addition of 100 mM NaCl to the nutrient solution were investigated. Two concentrations of NPs (50 mg/L and 100 mg/L) were applied through foliar spray on pea leaves. Data showed that NPs prevent salt-induced membrane damage, growth inhibition, and the increase in hydrogen peroxide and lipid peroxidation. An analysis of the chlorophyll fluorescence curves revealed that TiO₂ NPs decreased the effects of NaCl on the reduction in the open photosystem II centers (corresponding with qp) and their efficiency (Φexc), as well as the activity of the oxygen-evolving complex (Fv/Fo). The co-treatment with TiO₂ NPs and NaCl also improved the photochemical energy conversion of photosystem II (Φ_PSII), alleviated the interaction of Q_A⁻ with plastoquinone, and enhanced electron transport activity and the rate of photosynthesis, compared to the plants treated with NaCl only. Additionally, NPs application under salt stress stimulated cyclic electron transport around photosystem I, thus protecting its photochemical activity. These protective effects of NPs were more pronounced at a concentration of 100 mg/L. Full article

Morphological, anatomical, and molecular information facilitates the identification and inference of the relatedness of plant species. In this study, the macromorphological, micromorphological, and anatomical characteristics of nine species from the Brachychiton and Sterculia genera belonging to the Malvaceae family were examined by light and a scanning electron microscope. The study recorded 66 macromorphological, micromorphological, and anatomical characteristics, thus revealing important variations between the studied species in leaf morphology and anatomy. This included variations in leaf complexity, leaf arrangement (phyllotaxy), epidermal cell walls, and their sculpture, as well as in the types of glandular and non-glandular trichomes. The studied species were mostly conserved in shedding patterns, being evergreen only in one out of nine studied species. Similarly, eight species were petiolate. Conversely, leaf arrangement and leaf complexity characteristics were highly divergent among the studied species, though only one species, Sterculia foetida, had compound leaves. The differences in the studied features and the chloroplast genes MaturaseK (MatK) and ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcl) were exploited to deduce the relationship between the studied species. While the morphological and anatomical features demonstrated a close relationship between the studied intrageneric species, the DNA barcoding analysis proved very efficient in distinguishing the two neighboring genera. Collectively, the different clustering analyses suggest a close relatedness between Brachychiton acerifolius and B. australis, while only DNA-based clustering demonstrates cladistic monophyly of the Sterculia species. This study, therefore, provides a detailed description of various morphological and anatomical features important for the systematic studies within the Malvaceae family and highlights the value of incorporating morphological, anatomical, and molecular approaches for inferring the evolutionary relationship between closely related plant species. Full article

The Na⁺/H⁺ antiporter (NHX) gene family plays a pivotal role in plant salt tolerance in regulating intracellular Na⁺ and H⁺ homeostasis. In this study, seven candidate OsNHX genes (OsNHX1 to OsNHX7) were identified in the rice genome and classified into three phylogenetic clusters (Vac, Endo, and PM) based on their predicted subcellular localization. Five OsNHX gene pairs (OsNHX1/OsNHX2, OsNHX1/OsNHX3, OsNHX1/OsNHX4, OsNHX2/OsNHX6, and OsNHX5/OsNHX6) were found to have arisen from dispersed duplication events and exhibited purifying selection, indicating functional conservation. Analysis of cis-regulatory elements (CREs) revealed a diverse range of elements associated with tissue-specific expression, hormone signaling, and stress responses, particularly to dehydration and salinity. Notably, CREs associated with tissue/organelle-specific expression and stress responses were the most abundant, suggesting a potential role for OsNHX genes in regulating growth, development, and stress tolerance in rice. Importantly, expression profiling revealed that OsNHX1, OsNHX2, OsNHX3, and OsNHX5 were upregulated under salt stress, with significantly higher expression levels in the salt-tolerant rice cultivar Pokkali compared to the salt-sensitive cultivar IR64. Our findings provide a comprehensive analysis of the evolutionary, structural, and functional features of the OsNHX gene family and highlights their critical role in rice salt tolerance, offering insights into potential applications for crop improvement. Full article

In agriculture, abiotic and biotic stress reduce yield by 51–82% and 10–16%, respectively. Applications of biological agents such as plant growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) can improve plant growth. Applications of lone PGPR and AMF also help plants resist abiotic and biotic stressors. The reports for dual inoculation of AMF and PGPR to benefit plants and tackle stressors are largely unknown. It is speculated that PGPR colonization in plants enhances AMF infection during dual AMF and PGPR application, although increased AMF colonization does not always correlate with the increased benefits for the plant hosts. Further research is needed regarding molecular mechanisms of communication during dual inoculations, and dual-inoculation enhancement of induced systemic resistance under pathogen stress, to understand how dual inoculations can result in enhanced plant benefits. The influence of application timing of AMF and PGPR dual inoculations on mitigating abiotic and biotic stress is also not well understood. This review documents the factors that govern and modulate the dual application of AMF and PGPR for plant benefits against stress responses, specifically abiotic (drought) stress and stress from pathogen infection. Full article

The vulnerability of commercial crops under a changing climate has led scientists to consider wild crop species as alternative food sources. The aim of this study was to identify plastic physiological and morphological traits that could be used to in vitro screen Amaranthus dubius and Galinsoga parviflora seeds for drought, salinity, and heat tolerance. To establish the lethal dose/temperature, 50% (LD/T₅₀), for each stress, seeds for both were subjected to various mannitol and NaCl stresses and a range of temperatures. Percentage seedling emergence was selected as the initial indicator of tolerance and used to establish the LD/T₅₀ for in vitro screening for both species. Seeds of both were then screened at the LD/T₅₀ concentrations/temperatures established, and seedlings that emerged after 21 days were measured for leaf area, root (RL), shoot length (SL), chlorophyll content (Chl), fresh, dry mass, and leaf number. Data for these were used to quantify plasticity in terms of Valladares’s phenotypic plasticity index. For A. dubius, three (viz. RL, SL, and Chl) showed some plasticity (≥0.53) and tolerance across all three stressors. For G. parviflora all traits except SL showed some plasticity (≥0.58) and tolerance across all three stressors. Both species had high phenotypic plasticity across all three stressors, which suggests that wild leafy vegetables may possess the ability to tolerate climate change-associated stressors and should be considered for future breeding programs. Full article

Sugarcane mosaic virus (SCMV), sugarcane streak mosaic virus (SCSMV), and sorghum mosaic virus (SrMV) are the causative pathogens of mosaic disease. This study aimed to identify mosaic virus infection and its impact on photosynthetic and antioxidant gene expression in eight commercial sugarcane cultivars grown on sugarcane plantations in East Java, Indonesia. The disease incidence and severity were observed in symptomatic leave samples, and then the virus was identified. A high incidence and severity of mosaic symptoms were observed in the PS881 and NX04 cultivars compared with the other cultivars. RT-PCR analysis detected SCSMV infection in all cultivars; double infections with SCSMV and SCMV in the PS881, PS882, and Cening cultivars; and triple infections with SCSMV, SCMV, and SrMV in the PS881 cultivar. Ascorbate peroxidase (Apx) expression was upregulated in all virus-infected cultivars and significantly increased in the triple-infected PS881 cultivar. However, catalase (Cat) expression was only slightly increased in the PS881 cultivar. The chlorophyll content was reduced, and the PsaA gene was downregulated in all cultivars. The expression of PsaA, RbcS, and Sps was significantly suppressed in the triple-infected PS881 cultivar. Moreover, the downregulation of both the RbcS and Pepc genes was concomitant with that of their protein levels. Full article

Potato virus Y (PVY) is one of the most important constraints to potato production worldwide. There is an increasing occurrence of recombinant PVY strains PVY^NTN and PVY^N-Wi and a decline in the incidence of the nonrecombinant PVY^O. We hypothesized that this may be due to the ability of these recombinant strains to antagonize and/or outcompete PVY^O in mixed infections. To determine this, we investigated interactions between PVY^O and three recombinant PVY strains common in North America: PVY^NTN, PVY^N-Wi, and PVY^N:O. Overall, our study showed that these interactions are tissue-dependent. Specifically, PVY^NTN, the main causal agent of potato tuber necrotic ringspot disease (PTNRD), was found to be more adaptable than PVY^O, especially in potato leaves due, at least in part, to the Ny gene that confers hypersensitive resistance (HR) to PVY^O. Furthermore, PVY^N-Wi was found to repress PVY^O in potato tubers but act synergistically in potato leaves. The PVY^O-induced foliage necrosis in cultivar ‘Ranger Russet’ was observed to be more severe in plants co-infected by PVY^N-Wi and PVY^N:O, respectively, resulting in plant death. Strikingly, this PVY^O -induced necrosis was suppressed by PVY^NTN in doubly infected plants. These interactions may, at least partially, explain the decreasing incidence of PVY^O in United States potato production regions, especially given that many cultivars contain the Ny gene, which likely limits PVY^O enabling PVY^NTN and PVY^N-Wi to outcompete. We also found that replication and cell-to-cell movement of these PVY strains in tubers at 4 °C was similar to levels at ambient temperature. Full article

Aeollanthus repens, native to East Africa, thrives in seasonally dry tropical biomes and boasts qualities ideal for both ornamental and ground cover purposes. However, despite its potential, its current resistance levels remain uncertain. Assessing its adaptability could offer valuable insights for its wider adoption and utilization. In this study, researchers employed 3-month-old cuttings of A. repens, subjecting them to six distinct environments by manipulating the temperature (25/20 °C and 35/30 °C) and soil moisture levels (100%, 20%, and 40%). Their leaf physiological and photosynthetic indices were assessed at intervals of 5, 10, and 15 days following exposure to stress. The findings unveiled that exposure to prolonged moisture, elevated temperatures, or a combination of both led to an increase in osmoregulatory substances in the leaves. This increase was accompanied by heightened enzyme activity and an increased intercellular carbon dioxide concentration, followed by a subsequent decline. Additionally, chlorophyll content, net photosynthetic rate, stomatal conductance, and transpiration rate exhibited a decreasing trend over time. Through a comprehensive assessment of stress tolerance utilizing a composite affiliation function value index, the study concluded that A. repens exhibits optimal growth in a certain high-temperature environments and demonstrates substantial resistance to waterlogging, drought, and simultaneous high-temperature stress. However, the resilience of A. repens appears to diminish under the compounded stresses of high temperature and drought. Full article

Biodiversity and sustainable development in Sub-Saharan Africa (SSA) are considerably impacted by invasive alien plants (IAPs). Increasing plant invasions in SSA threaten agricultural productivity, biodiversity conservation, and other socioeconomic activities, which in turn put the United Nations Sustainable Development Goals (SDGs) in peril. In order to effectively combat IAPs, understanding their functional traits (morphological, physiological, and phenological traits) and integrating them into remote sensing (RS) is vital. While functional traits influence IAPs’ fitness to invade and establish in a new geographical range, RS aids in studying them remotely, delineating and mapping them, and predicting their potential invasions. The information on this study topic was gathered by reviewing various existing studies published between 2000 and 2024. Based on this review, it was deduced that the majority of IAPs are fast-growing (or acquisitive), with a shorter leaf lifespan, bigger leaves, and higher plant height, ultimately resulting in a higher resource acquisition ability. We established further that in SSA, there are limited studies on IAP functional traits and their integration in RS. Many studies conducted in the region focus mostly on IAP distribution. Evidence from prior studies revealed that functional trait remote sensing (FTRS)-based research not only improves detection and mapping but also predicts whether a certain alien plant can become invasive or expand its distribution range. Thus, using the FTRS approach could help IAP management in SSA, ultimately achieving the SDGs. Our review discusses IAP implications in SSA (e.g., Angola, Tanzania, Benin, Kenya, Uganda, Rwanda, Zambia, Burundi, Zimbabwe, Botswana, Malawi, etc.) and for the achievement of SDGs; functional traits and their impact on alien invasions; and the importance of incorporating functional traits into RS. Full article

Soybean is a high-value food source, and the invasive weeds Mexican prickly poppy (Argemone ochroleuca) could release allelochemicals that inhibit the growth of this crop. The impact of A. ochroleuca on the germination and growth of soybean is not well documented. Therefore, the aim of this study was to evaluate the TLC profiles of different extracts of A. ochroleuca and assess the effects of extracts on the germination of soybean seeds. Shoots and roots of A. ochroleuca were weighed and 100 g of each was separately extracted with 1000 mL deionized water, hexane or acetone. Ten concentrations of water extracts ranging from 10 to 100 mL per 100 mL of deionized water and three concentrations of acetone and hexane extracts ranging from 2.5 to 7.5 g/L were separately used for seed germination bioassays. Thin-layer chromatography (TLC) analysis was used to compare the chemical profiles in the shoot and root water, and in the hexane and acetone extracts of A. ochroleuca. The highest reduction was recorded from the water extract, at 100%. The TLC profiling of A. ochroleuca addressed different classes of compounds, including alkaloids, phenolic acids and flavanoids. There is, however, a need to identify the most active phytochemicals in the suppression of germination. Full article