Search Results (169)

Large soil pores critically influence water and solute transport in soils. The presence of preferential flow paths created by soil macropores can profoundly impact water quality, underscoring the necessity of accurately assessing the characteristics of these macropores. However, it remains unclear whether variations in macropore structure exist between different altitudes and positions of terraced paddy fields. The primary objective of this research was to utilize X-ray computed tomography (CT) and image analysis techniques to characterize the soil pore structure at both the inner field and ridge positions across different altitude levels (high, medium, and low altitude) within terraced paddy fields. The results indicate that there are significant differences in the distribution of large soil pores at different altitudes, with large pores concentrated in the surface layer (0–10 cm) in low-altitude areas, while in high-altitude areas, the distribution of large pores is more uniform. Additionally, as altitude increases, the porosity of large pores shows an increasing trend. The three-dimensional equivalent diameter and large pore volume are primarily characterized by large pores ranging from 1 to 2 mm and 0 to 5 mm³, respectively, with their morphology predominantly appearing spherical or ellipsoidal. The connectivity of large pores in the surface layer of paddy soil is stronger than that in the bunds. However, this connectivity gradually weakens with increasing soil depth. The findings from this study provide valuable quantitative insights into the unique characteristics of soil macropores that vary according to the altitude and position in terraced paddy fields. Moreover, this study emphasizes the necessity for future research that encompasses a broader range of soil types, altitudes, and terraced paddy locations to validate and further explore the identified relationships between altitude and macropore characteristics. Full article

In the context of critical challenges in curcumin-modified polyurethane synthesis—including limited curcumin bioavailability and suboptimal biodegradability/biocompatibility—a novel polyurethane material (Cur-PU) with good mechanical, shape memory, pH-responsive, and biocompatibility was synthesized via a one-pot, two-step synthetic protocol in which HO-PCL-OH served as the soft segment and curcumin was employed as the chain extender. The experimental results demonstrate that with the increase in Cur units, the crystallinity of the Cur-PU material decreases from 32.6% to 5.3% and that the intensities of the diffraction peaks at 2θ = 21.36°, 21.97°, and 23.72° in the XRD pattern gradually diminish. Concomitantly, tensile strength decreased from 35.5 MPa to 19.3 MPa, and Shore A hardness declined from 88 HA to 65 HA. These observations indicate that the sterically hindered benzene ring structure of Cur imposes restrictions on HO-PCL-OH crystallization, leading to lower crystallinity and retarded crystallization kinetics in Cur-PU. As a consequence, the material’s tensile strength and hardness are diminished. Except for the Cur-PU-3 sample, all other variants exhibited exceptional shape-memory functionality, with R_f and R_r exceeding 95%, as determined by three-point bending method. Analogous to pure curcumin solutions, Cur-PU solutions demonstrated pH-responsive chromatic transitions: upon addition of hydroxide ion (OH⁻) solutions at increasing concentrations, the solutions shifted from yellow-green to dark green and finally to orange-yellow, enabling sensitive pH detection across alkaline gradients. Hydrolytic degradation studies conducted over 15 weeks in air, UPW, and pH 6.0/8.0 phosphate buffer solutions revealed mass loss <2% for Cur-PU films. Surface morphological analysis showed progressive etching with the formation of micro-to-nano-scale pores, indicative of a surface-erosion degradation mechanism consistent with pure PCL. Biocompatibility assessments via L929 mouse fibroblast co-culture experiments demonstrated ≥90% cell viability after 72 h, while relative red blood cell hemolysis rates remained below 5%. Collectively, these findings establish Cur-PU as a biocompatible material with tunable mechanical properties, and pH responsiveness, underscoring its translational potential for biomedical applications such as drug delivery systems and tissue engineering scaffolds. Full article

Ammonia, as a toxic and corrosive gas, is widely present in industrial emissions, agricultural activities, and disease biomarkers. Detecting ammonia is of vital importance to environmental safety and human health. Sensors based on MXene have become an effective means for detecting ammonia gas due to their unique hierarchical structure, adjustable surface chemical properties, and excellent electrical conductivity. This study reviews the latest progress in the use of MXene and its composites for the low-temperature detection of ammonia gas. The strategies for designing MXene composites, including heterojunction engineering, surface functionalization, and active sites, are introduced, and their roles in improving sensing performance are clarified. These methods have significantly improved the ability to detect ammonia, offering high selectivity, rapid responses, and ultra-low detection limits within the low-temperature range. Successful applications in fields such as industrial safety, food quality monitoring, medical diagnosis, and agricultural management have demonstrated the multi-functionality of this technology in complex scenarios. The challenges related to the material’s oxidation resistance, humidity interference, and cross-sensitivity are also discussed. This study aims to briefly describe the reasonable design based on MXene sensors, aiming to achieve real-time and energy-saving environmental and health monitoring networks in the future. Full article

With the rapid expansion of the aquaculture scale, the environmental pollution caused by the accumulation of fish pond sediment (FPS) has become increasingly prominent, making it urgent to establish sustainable resource utilization solutions. This study investigates the potential of using FPS as a soil amendment to improve saline–alkali soil (SAS) quality and enhance vegetable growth, while also quantifying ecological benefits through Gross Ecosystem Product (GEP) accounting. A pot experiment was conducted to evaluate the effects of different FPS mass percentages (0%, 20%, 40%, 80%, and 100%) on the growth of three vegetables (water spinach, lettuce, and chili) and soil quality. The results demonstrated that FPS addition at ≥40% significantly improves SAS quality, reducing the pH and salinity (p < 0.05), while enhancing organic matter, nutrient availability, and microbial activity. Among the treatments, 80% FPS maximized vegetable yields, with water spinach achieving the highest edible biomass (37.32 g). Compared to the control, nutritional quality under ≥80% FPS treatment showed substantial increases: vitamin C (133.33–307.03%), soluble sugars (49.97–73.53%), and protein (26.14–48.08%). An economic analysis revealed that 80% FPS with water spinach cultivation generated peak ecological benefits (274,951 CNY·ha⁻¹; 185% above control). These findings provide a scientific basis and effective model for the resource utilization of FPS and the improvement of saline–alkali soil, offering significant implications for the sustainable development of agriculture and environmental protection. Full article

The Northeastern China Gravity Monitoring Network (NCGMN; 40–50°N), a pioneering time-variable gravity monitoring system in high-latitude cold-temperate environments, serves as a critical infrastructure for geodynamic investigations of the Songliao Basin, Changbai Mountain volcanic zone, and northern Tan-Lu Fault Zone. To address the data reliability challenges posed by nonlinear dynamic drifts in spring-type relative gravimeters during mobile surveys, this study quantifies—for the first time—the non-smooth normal distribution characteristics of such drifts using the inaugural 2015 dataset from two CG-5 instruments. Results demonstrate a 7–15% reduction in mean dynamic drift rates compared to static conditions, with spatiotemporal variability governed by multi-physics field coupling (terrain undulation, thermal fluctuation, and barometric perturbation). A comprehensive correction framework—integrating a gravimetric line drift rate computation, multi-model validation, and absolute datum cross-validation—reveals gravity value discrepancies up to ±10 μGal across models. The innovative hybrid scheme combines local drift preprocessing (initial-point modeling, line fitting, variance-sum optimization) with global adjustment optimization, achieving the significant suppression of nonlinear drift errors. The variance-sum optimal and Bayesian adjustment hybrid synergizes local variance minimization and global temporal correlation priors, delivering the following: (1) 34% and 29% reductions in segment self-difference standard deviations versus classical and Bayesian adjustments; (2) 24% and 14% decreases in segment residual standard deviations; (3) 12% and 6% improvements in absolute datum cross-validation precision. This study establishes a foundation for the reliable extraction of μGal-level gravity signals, advancing high-precision gravity monitoring of seismicity, volcanic unrest, and fault zone deformation in complex terrains. By harmonizing local-scale accuracy with network-wide consistency, the framework sets a new benchmark for time-variable gravity studies in challenging environments. Full article

River pollution is a major issue in China’s urbanization process. Understanding the effects of river morphology and constructed wetlands on the self-purification capacity is crucial for water quality improvement. This study takes the Shiwuli River, a main tributary of Chaohu Lake, as an example. By monitoring the concentration changes of five water quality indicators—total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH₃-N), chemical oxygen demand (COD), and dissolved oxygen (DO)—in the river section for the years 2017 and 2024, we conducted a comparative analysis of the relationship between river morphology and self-purification capacity, as well as influencing factors. The results show that meandering rivers possess self-purification capabilities under natural conditions. There is a positive correlation between river sinuosity and the reduction rates of TP, TN, NH₃-N, and COD, as well as the increase rate of DO—the greater the sinuosity, the stronger the purification capacity. Wetlands enhance both the self-purification capacity and the purification rate of river channels, reducing the required sinuosity for effective self-purification from 1.49 to 1.30. This study also discusses the mechanisms by which meandering rivers influence water self-purification, and proposes that increasing river sinuosity and constructing wetlands can enhance the self-purification capacity. This measure will increase the length and width of the river, prolong the purification time, improve the DO level, and enhance the exchange between the riverbed and groundwater. The findings of this study can provide a reference for river restoration and management in the context of urbanization. Full article

The lethality of esophageal squamous cell carcinoma (ESCC), and other epithelial cancers, is primarily due to its aggressive nature and frequent lymphatic metastasis, both of which impact prognosis. In this review, we explore the underlying molecular mechanisms of ESCC lymphatic metastasis, specifically, the functional role of NRF2 and therapeutic strategies. Current data suggest that NRF2 hyperactivation (NRF2^high) may promote lymphatic metastasis of ESCC by affecting the extracellular matrix (ECM), epithelial–mesenchymal transition (EMT), lymphangiogenesis, immune evasion, metabolic programming, and Hippo signaling. We also update the latest developments in NRF2 inhibitors, their mechanisms of action, screening strategies, and approaches for evaluating compound efficacy. Finally, we highlight the utility of animal models for mechanistic studies and therapeutic development. We believe elucidation of the functional role of NRF2 in ESCC lymphatic metastasis and developing proper NRF2 inhibitors will greatly improve the clinical prognosis of ESCC in human patients. Full article

Office buildings often consume a large amount of energy during their operational phase, primarily due to insufficient consideration of the coordination among energy consumption, thermal comfort, and visual comfort in the design process. This study employs a multi-objective genetic algorithm to optimize the overall performance of office buildings by parameterizing seven key design variables: floor plan aspect ratio, building orientation angle, window-to-wall ratios (WWRs) in all directions, shading strategy, shading device orientation, shading device length, and shading device spacing. A building performance simulation model was established to conduct a global optimization search, with simultaneous analysis across the east, south, west, and north façades to obtain a set of Pareto-optimal solutions that satisfy multiple performance objectives. The results indicate that optimal comprehensive performance across energy use, thermal comfort, and visual comfort can be achieved under the following conditions: a floor plan aspect ratio of 0.67–1, building rotation of 0–20° clockwise, an east-facing WWR of 0.4, south- and west-facing WWRs of 0.2–0.4, and a north-facing WWR of 0.4–0.6. For shading, horizontal devices with a length of 0.8–1.0 m, downward tilt angle of 10–30°, and spacing of 0.6–1.2 m are recommended. These findings provide scientific parameter references and optimization pathways for the design of high-performance office buildings in various climate conditions. Full article

The overuse of pesticides has led to resistance in phytopathogens, posing significant threats to global food security and environmental health. Antimicrobial peptides (AMPs), small molecules produced by various organisms as part of their innate immune defense, exhibit broad-spectrum antimicrobial activity with a lower risk of resistance development. These properties make AMPs promising candidates for sustainable agricultural practices. However, challenges such as high production costs, instability, and potential toxicity to plant cells have hindered their widespread application. This review provides a comprehensive overview of the discovery, classification, and antimicrobial mechanisms of AMPs, focusing on their roles in plant protection. It also explores strategies for identifying and optimizing AMPs, including structural modifications, targeted delivery systems, and production methods using plant- and microbe-based expression systems. Additionally, the review highlights the potential of transgenic approaches to enhance crop resistance by expressing AMP genes in plants. Despite the challenges, AMPs offer a transformative opportunity for modern agriculture, providing innovative solutions to combat plant diseases while reducing reliance on conventional pesticides. Continued research and technological advancements are essential to fully realize the potential of AMPs in sustainable plant protection. Full article

Preventing loss of nitrogen during aerobic manure composting is a critical challenge, and introducing microbial agents with specific functions offers a promising solution. This study aimed to explore how Bacillus subtilis F2 (a thermotolerant nitrifying bacterium) affects nitrogen conservation, microbial dynamics, and nitrogen conversion-associated gene abundance during pig manure composting. Relative to the uninoculated controls, adding F2 markedly raised the germination index, nitrate content, and total nitrogen in the final compost, resulting in reduced nitrogen loss. The inoculation led to a distinct succession of bacterial communities, enriching microorganisms associated with fermentation and hydrocarbon degradation, while the fungal communities did not change significantly between the control and treated compost. Furthermore, inoculation markedly increased amoA gene levels and decreased nirK abundance during the cooling and maturation phases. Significant relationships were detected between nitrogen content, microbial composition, and nitrogen conversion genes in correlation analyses. In summary, the addition of F2 is recommended for bolstering nitrogen retention in the context of composting. Full article

Large-scale penetration of renewable energy generation brings various challenges to the power system in terms of safety, reliability, economy and flexibility. The development of large-scale, high-security energy-storage technology can effectively address these challenges and improve the capabilities of power systems in power-supply guarantee and flexible adjustment. This paper proposes a novel distributed risk-averse optimization scheduling model of a hybrid wind–solar–storage system based on the adjustability of the storage system and the complementarity of renewable energy generation. The correlation of wind power and photovoltaic generation is quantified based on a Copula function. A risk-averse operation optimization model is proposed using conditional value at risk to quantify the uncertainty of renewable energy generation. A linear formulation of conditional value at risk under typical scenarios is developed by Gibbs sampling the joint distribution and Fuzzy C-Means clustering algorithm. A distributed solution algorithm based on an alternating-direction method of multipliers is developed to derive the optimal scheduling of hybrid wind–solar–storage system in a distributed manner. Numerical case studies based on IEEE 34-bus distribution network verify the effectiveness of the proposed model in reducing the uncertainty impact of renewable energy generation on an upstream grid (the overall amount of renewable energy generation sent back to the upstream grid has decreased about 80.6%) and ensuring the operational security of hybrid wind–solar–storage system (overall voltage deviation within 5.6%). Full article

Natural variations conferring salt tolerance (ST) are of great value for breeding salt-tolerant rice varieties. The major ST genes, including SKC1, RST1, OsWRKY53 and STG5, have been identified to contain or be associated with a specific single nucleotide polymorphism (SNP). However, the distribution and genetic effects of those ST genes in rice cultivars remain poorly understood. Here, we investigated the distribution of seven cloned ST genes, including SKC1 (P140A, R184H), RST1 (A530G, E611G), OsWRKY53 (A173G), STG5 (I12S), OsHKT1;1 (L94K), OsHKT2;3 (I77T) and OsSTL1 (P289S), which contain one or two ST-related SNPs in a sequenced Indica/Xian rice population comprising 550 accessions. On the basis of the SNPs, the population was categorized into 21 haplotypes (Haps), each of which contained at least four out of seven ST genes. To precisely evaluate each SNP, grouped rice varieties that only differed at one SNP were chosen from two Haps for salt treatment with 150 mM NaCl for 7 d. The results revealed that RST1^611G showed up to 88.6% improvement in salt tolerance considering the relative shoot fresh weight (rSFW). Alternatively, OsWRKY53^173G, OsHKT2;3^77T, SKC1^140A and SKC1^184H showed an improvement in rSFW of 38.6%, 37%, 27.5% and 19.0%, respectively, indicating that they contribute different genetic effects for ST. OsHKT1;1^94K showed no function with salt treatment for 7 d, but showed a 37.9% rSFW improvement with salt treatment for 14 d. Furthermore, we found that the expression of OsWRKY53^173G was positively correlated with SKC1 and conditionally participated in ST dependent on SKC1^140A. Interestingly, RST1^530A was previously reported to be associated with salt sensitivity, but it was found to be associated with salt tolerance in this study. Overall, our results provide further insight into the mechanism and marker-assisted selection improvement of ST in Indica/Xian rice. Full article

Background: Coronary artery bypass grafting (CABG) is one of the main treatments for coronary heart disease (CHD). Gut microbiota, including bacteria, fungi, archaea, and virus, has been reported to be associated with CHD. However, the changes in the multi-kingdom gut microbiota after CABG are not yet clear. This study aimed to explore the changes in multi-kingdom gut microbiota during the early postoperative period of CABG. Methods: We collected fecal samples from 40 patients before and 1 week after CABG surgery. Metagenomic sequencing was used to detect the microbial spectrum and gene functions in the patients’ fecal samples. Results: Post-CABG patients exhibited significant changes in the composition of multi-kingdom gut microbiota and gene functions. Among bacteria, beneficial species such as Bifidobacterium, Bacteroides, and Blautia were significantly reduced after CABG, while the harmful species Enterococcus was significantly increased. In fungi, Schizosaccharomyces pombe was significantly decreased in the postoperative group, while Saccharomyces cerevisiae and Aspergillus chevalieri were significantly increased postoperatively. Spearman correlation analysis indicated that Schizosaccharomyces pombe had positive interactions with beneficial bacteria such as Lachnospiraceae, Ruminococcus, and Blautia. Among archaea, the preoperatively enriched Methanomethylovorans-SGB40959 was significantly reduced postoperatively, and Spearman correlation analysis showed a significant positive interaction with probiotics Ruminococcus and Dorea. In viruses, the phage Enterococcus virus EFP01, which infects Enterococcus, was significantly increased postoperatively and showed a significant positive interaction with Enterococcus. Additionally, postoperative dysregulation of gene functions such as the Phosphoenolpyruvate-dependent Sugar Phosphotransferase System (PTS), Transposition, DNA-mediated, and Transposase Activity was observed, and Spearman correlation analysis indicated significant correlations between the dysregulated gene functions and the microbial communities. Conclusions: This study comprehensively revealed the changes in multi-kingdom species post-CABG. The reduction of beneficial microorganisms and the increase of harmful microorganisms after surgery are of significant clinical importance for understanding the overall health status of post-CABG patients and for optimizing postoperative treatment plans. Future research needs to further explore how to improve the prognosis of post-CABG patients by modulating the gut microbiota. Full article

Amidst the global energy crisis and progress in clean energy, this study aims to reduce design costs and improve the adaptability of turbines in small ORC systems. It seeks to offer enhanced renewable energy utilization methods for sustainable development. This paper focuses on the performance of an impulse single-stage turbine with partial admission and analyzes it through numerical simulations using computational fluid dynamics (CFD). The study investigates critical parameters under design and off-design conditions by varying inlet total pressure and rotor speed. The results indicate that the turbine’s isentropic efficiency and power output at design conditions are 64% and 4.78 kW, respectively. The power output ranges from 4.65 kW to 6.81 kW, and the isentropic efficiency ranges from 57% to 62% under off-design conditions. Both experimental and simulation results show good agreement. Furthermore, the velocity triangles under these conditions conform to those of a pure impulse turbine. These findings demonstrate that the turbine could adapt to different conditions and facilitate the design of ORC systems. Full article

Introduction: Metabolic dysfunction-associated steatohepatitis (MASH) is a leading cause of liver failure globally and is characterized by hepatic steatosis and inflammation, which may progress to fibrosis, the severity of which is highly predictive of patient demise and death. In view of the lack of treatment options for MASH, we investigated the therapeutic properties of extracellular vesicles (EVs) from normal human hepatocytes, which we have previously been shown to alleviate toxin-mediated hepatic fibrosis in mice. Methods: C57BI/6J mice were fed a choline-deficient amino acid-defined high (60%) fat (CDAA-HF) diet for up to 12 weeks while receiving i.p. administration of EVs purified from cultured human HepG2 hepatocytes. Results: CDAA-HF diet consumption resulted in severe hepatic steatosis, increased frequency of CD45+ lymphocytes and F4/80+ macrophages, robust production of aortic smooth muscle actin (ACTA2), and deposition of interstitial collagen, as well as altered serum levels of ALT, AST, cholesterol, triglycerides, alkaline phosphatase, unconjugated bilirubin, and total protein, thus recapitulating typical MASH phenotypes. EVs administered preventively or therapeutically resulted in the restoration of serum marker levels, reduced hepatic inflammation and attenuation of collagen deposition, ACTA2 production, and expression of fibrosis-associated genes. HepG2 EVs contained 205 miRs and, among the 30 most abundant miRs, seven (miRs-423-5p, -483-5p, -191-5p, -148a-3p, -423-3p, -92a-3p, -122-5p) are predicted to directly target fibrosis-related genes (collagens, ACTA2, MMPs, and TIMPs). Conclusions: Hepatocyte EVs are therapeutic in a mouse model of diet-induced steatohepatitis with fibrosis. Further studies of hepatocyte EVs or their cargo components as novel therapeutics for MASH in humans are warranted, including treatment of fibrotic stages, which are associated with clinical demise and are predictive of patient death. Full article