Search Results (17)

The yak industry constitutes a pivotal segment of livestock development across the Qinghai–Tibetan Plateau and adjacent pastoral regions. Existing studies have shown that supplemental feeding for grazing yaks significantly improves meat quality and growth performance, but its effect on enteric methane (CH₄) emission from yaks has never been reported, so the present experiment aimed to investigate the effect of supplemental feeding of grazing yaks on CH₄ emissions in three different periods of the warm season. Thirty male yaks were randomly assigned to two groups, with 15 yaks per group, The groups were designated as the grazing group (GR), with traditional grazing methods and with an initial body weight of 94.56 ± 3.9 kg, and the supplemental feeding group (GRS) with an initial body weight of 95.01 ± 4.1 kg, which received 1500 g/d of supplemental feed for 120 days. The methane emissions of the two groups were measured at the late regreening period (LRP), the greening-grass period (GGP), and the browning period (BRP) using the SF₆ tracer method. The results showed that GRS increased (p < 0.01) the total DMI during warm season but decreased (p < 0.01) the forage intake at the LRP and GGP, resulting in a significantly reduced methane yield per kg of BW gain and per kg of DMD in warm-season grazing yaks (p < 0.01), a significantly reduced methane production per kg of DMI and methane energy to gross energy ratio in grazing yaks during the GGP (p < 0.05), and a similarly significantly decrease in methane production per kg of DMI and methane energy to gross energy ratio in grazing yaks during the BRP (p < 0.01). In conclusion, supplemental nutrition for grazing yaks during the warm season in alpine grasslands significantly enhances growth performance, reduces methane emissions, and improves dietary energy utilization efficiency. Full article

This study investigates the effect of altitude on the fatty acid composition of pasture grass, yak milk, and yak ghee on the Qinghai–Tibet Plateau, aiming to understand how environmental factors influence the nutritional quality of these products. Samples were collected from four different altitudes and analyzed for fatty acid profiles using gas chromatography. The analysis reveals that higher altitudes are associated with an increased prevalence of beneficial unsaturated fatty acids, such as oleic acid (C18:1) and linoleic acid (C18:2n6c). These findings highlight the significant influence of altitude on yak lipid metabolism, ultimately enhancing the nutritional value of dairy products. This adaptation not only supports the health and resilience of yaks, but also provides vital nutritional benefits to residents in high-altitude regions. The research underscores the importance of further investigations to optimize dairy production practices, ensuring improved food security and health outcomes for residents of the plateau. Full article

Solar panel efficiency decreases with an increase in the panel surface temperature. This study utilized the Phase Change Material (PCM) based cooling approach along with Aluminum fins to reduce the temperature of the PV panel. The PV panel surface temperature and efficiency are the target parameters we investigated. The results were compared with conventional PV panel values at inclination angles of 30°, 45°, and 60° with respect to the ground. The experimental results show that the PV panel efficiency increased by 6.85%, 6.82%, and 4.2% for the inclination angles of 30°, 45°, and 60°, respectively. The corresponding maximum temperature drops were 13.1 °C, 12.9 °C, and 8.5 °C. Full article

Depression is the major mental illness which causes along with loss of interest in daily life, a feeling of hopelessness, appetite or weight changes, anger and irritability. Due to the hepatic first-pass metabolism, the absolute bioavailability of fluvoxamine (FVM) after oral administration is about 50%. By avoiding the pre-systemic metabolism, nasal delivery would boost bioavailability of FVM. Additionally, the absorption is anticipated to occur more quickly than it would via the oral route because of the existence of microvilli and high vasculature. A nonionic surfactant, cholesterol and an arachidonic acid-carboxymethyl chitosan (AA-CMCS) conjugate were used to develop FVM-loaded novasomes. To investigate the effects of surfactant concentration, AA-CMCS conjugate concentration and stirring speed on the novasomes’ characteristics, a Box–Behnken design was used. The dependent variables chosen were zeta potential, polydispersity index and particle size. The AA-CMCS conjugate was confirmed by ¹H-NMR and FTIR. Using Design Expert software (version 7; Stat-Ease Inc., Minneapolis, MN, USA), novasomes were further optimized. The chosen optimal formulation (NAC8) was made up of AA-CMCS conjugate, Span 60 and cholesterol. Particle size, zeta potential and PDI values for NAC8 formulation were 101 nm, −35 mV and 0.263, respectively. The NAC8 formulation’s DSC and TGA analysis demonstrated that the medication had been uniformly and amorphously distributed throughout the novasomes. The NAC8 formulation showed 99% and 90% FVM release and permeation, respectively, and the novasome adherence time was 24 h. An improved antidepressant effect along with five-fold increase in bioavailability of FVM was observed after trans-nasal administration of NAC8 formulation compared to the reference commercially available Flumin^® tablets. FVM-loaded novasomes administered via the nasal route may therefore constitute an advancement in the management of depression. Full article

A hydrogel topical patch of neomycin was developed by using sodium alginate (SA) and hydroxyethylcellulose (HEC) as polymers. Free radical polymerization in an aqueous medium was initiated by using acrylic acid (AA) and N,N′-methylenebisacrylamide (MBA). Prepared hydrogels were characterized for pH sensitivity and sol–gel analysis. In addition, the effect of reactant contents on the developed formulation was evaluated by swelling behavior. SEM assay showed the rough structure of the hydrogel-based polymeric matrix, which directly enhances the ability to uptake fluid. FTIR spectra revealed the formation of a new polymeric network between reactant contents. TGA and DSC verified that fabricated polymeric patches were more thermodynamically stable than pure components. Gel fractions increased with increases in polymer, monomer, and cross-linker contents. The swelling study showed the pH-dependent swelling behavior of patches at pH 5.5, 6.5, and 7.4. The release pattern of the drug followed zero-order kinetics, with diffusion-controlled drug release patterns according to the Korsmeyer–Peppas (KP) model. Ex vivo studies across excised rabbit skin verified the drug retention in the skin layers. The hydrogel patch effectively healed the wounds produced on the rabbit skin, whereas the formulation showed no sign of irritation on intact skin. Therefore, neomycin hydrogel patches can be a potential candidate for controlled delivery for efficient wound healing. Full article

The growing pressures from the government, buyers, consumers, suppliers, and the general public have recently compelled manufacturing firms to enhance their production methods by becoming more environmentally friendly and pursuing new innovative methods for producing green goods in developing countries. However, the relationship between green supply chain management (GSCM), green human capital (GHC), green innovation (GIN), managerial environmental knowledge (MEK), and firm performance (FPR) has received only a minimal amount of research focus. This study addresses this research gap by providing empirical evidence to motivate firms to implement GSCM, GHC, GIN, and MEK to enhance their FPR in developing countries substantially. A conceptual framework was developed to connect the concepts mentioned above. A questionnaire-based survey was used to collect the data. A total of 736 respondents from manufacturing firms in China were selected. Utilizing structural equation modeling (SEM), the data were analyzed. The findings indicate that neither GHC nor GSCM directly affects FPR; rather, GIN mediates the connection between GHC, GSCM, and FPR. In addition, the findings showed that MEK directly affects FPR and moderates the link between GIN and FPR. This study examined several theoretical and managerial implications and expanded research in the related fields. The results may help practitioners and managers comprehend how GSCM practices impact GIN and FPR. The outcomes of this research will benefit experts, policy makers, and stakeholders who seek to encourage FPR enhancements. Full article

This study investigates the impact of an organization’s response to poaching on employee productivity while considering the mediating role of organizational agility, contributing to the sustainability literature. The study aimed to explore the impact of three responses toward poaching, including the constructive response (CR), defensive response (DR), and retaliatory response (RR), on employee productivity. The survey-based approach collected data from companies in the Pakistani service sector, an essential step toward understanding sustainable human resource practices in developing countries. Regression analysis was used to analyze the collected data, revealing significant relationships between predictor variables and employee productivity. Specifically, Model 1 found a significant negative relationship between CR and productivity, whereas DR showed a positive and significant association with productivity. The study also considered the unique characteristics of the Pakistani service sector to explain the observed negative and positive relationships between CR and DR, respectively, and employee productivity. Model 2 improved the model fit by introducing organizational agility as a predictor variable, demonstrating a positive and significant relationship between productivity and all four predictor variables. The study suggests that the positive relationship between organizational agility and productivity could be due to agile organizations being more adept at responding to changing market conditions and demands, resulting in improved overall performance. This study provides valuable insights into the impact of an organization’s response to poaching on employee productivity while considering the mediating role of organizational agility. It contributes to the sustainability literature. Full article

The ongoing antibiotic-resistance crisis is becoming a global problem affecting public health. Urgent efforts are required to design novel therapeutics against pathogenic bacterial species. Brucella melitensis is an etiological agent of brucellosis, which mostly affects sheep and goats but several cases have also been reported in cattle, water buffalo, yaks and dogs. Infected animals also represent the major source of infection for humans. Development of safer and effective vaccines for brucellosis remains a priority to support disease control and eradication in animals and to prevent infection to humans. In this research study, we designed an in-silico multi-epitopes vaccine for B. melitensis using computational approaches. The pathogen core proteome was screened for good vaccine candidates using subtractive proteomics, reverse vaccinology and immunoinformatic tools. In total, 10 proteins: catalase; siderophore ABC transporter substrate-binding protein; pyridoxamine 5′-phosphate oxidase; superoxide dismutase; peptidylprolyl isomerase; superoxide dismutase family protein; septation protein A; hypothetical protein; binding-protein-dependent transport systems inner membrane component; and 4-hydroxy-2-oxoheptanedioate aldolase were selected for epitopes prediction. To induce cellular and antibody base immune responses, the vaccine must comprise both B and T-cells epitopes. The epitopes were next screened for antigenicity, allergic nature and water solubility and the probable antigenic, non-allergic, water-soluble and non-toxic nine epitopes were shortlisted for multi-epitopes vaccine construction. The designed vaccine construct comprises 274 amino acid long sequences having a molecular weight of 28.14 kDa and instability index of 27.62. The vaccine construct was further assessed for binding efficacy with immune cell receptors. Docking results revealed that the designed vaccine had good binding potency with selected immune cell receptors. Furthermore, vaccine-MHC-I, vaccine-MHC-II and vaccine-TLR-4 complexes were opted based on a least-binding energy score of −5.48 kcal/mol, 0.64 kcal/mol and −2.69 kcal/mol. Those selected were then energy refined and subjected to simulation studies to understand dynamic movements of the docked complexes. The docking results were further validated through MMPBSA and MMGBSA analyses. The MMPBSA calculated −235.18 kcal/mol, −206.79 kcal/mol, and −215.73 kcal/mol net binding free energy, while MMGBSA estimated −259.48 kcal/mol, −206.79 kcal/mol and −215.73 kcal/mol for TLR-4, MHC-I and MHC-II complexes, respectively. These findings were validated by water-swap and entropy calculations. Overall, the designed vaccine construct can evoke proper immune responses and the construct could be helpful for experimental researchers in formulation of a protective vaccine against the targeted pathogen for both animal and human use. Full article

Background and Objectives: Hereditary multiple exostoses (HME) is a disease characterized by cartilage-capped bony protuberances at the site of growth plates of long bones. Functional mutations in the exostosin genes (EXT1 and EXT2) are reported to affect the hedgehog signalling pathways leading to multiple enchondromatosis. However, the exact role of each EXT protein in the regulation of heparan sulphate (HS) chain elongation is still an enigma. In this study, a Pakistani family with HME is investigated to find out the genetic basis of the disease. Materials and Methods: Genotyping of eight members of the family by amplifying microsatellite markers, tightly linked to the EXT1 and EXT2 genes. Results: The study revealed linkage of the HME family to the EXT1 locus 8q24.1. Sanger sequencing identified a heterozygous deletion (c.247Cdel) in exon 1 of EXT1, segregating with the disease phenotype in the family. In silico analysis predicted a shift in the frame causing an early stop codon (p.R83GfsX52). The predicted dwarf protein constituting 134 amino acids was functionally aberrant with a complete loss of the catalytic domain at the C-terminus. Interestingly, an alternative open reading frame 3 (ORF3) caused by the frame shift is predicted to encode a protein sequence, identical to the wild type and containing the catalytic domain, but lacking the first 100 amino acids of the wild-type EXT1 protein. Conclusion: Consequently, haploinsufficiency could be the cause of HME in the investigated family as the mutated copy of EXT1 is ineffective for EXT-1/2 complex formation. The predicted ORF3 protein could be of great significance in understanding several aspects of HME pathogenesis. Full article

Abiotic stresses, such as high temperature and drought conditions, greatly influence the development of plants and the quality and quantity of products. Barley (Hordeum vulgare L.) crop production is largely impacted by drought, affecting growth, yield, and ultimately the productivity of the crop in hot arid/semi-arid conditions. The current pot experiment was directed to observe the outcome of nicotinic acid (NA) treatments on barley’s physiological, biochemical, and production attributes at two capacity levels, i.e., 100% normal range and withholding water stress. Randomized complete block design (RCBD) was used during the experimentation with the two-factor factorial arrangement. NA was applied exogenously by two different methods, i.e., foliar and soil application (fertigation). NA solution contained various application levels, such as T1 = control, foliar applications (T2 = 0.7368 gL⁻¹, T3 = 1.477 gL⁻¹, T4 = 2.2159 gL⁻¹), and soil applications (T5 = 0.4924 gL⁻¹, T6 = 0.9848 gL⁻¹, and T7 = 1.4773 gL⁻¹). Results depicted that, overall, foliar treatments showed better effects than control and soil treatments. Plant growth was preeminent under T4 treatment, such as plant height (71.07 cm), relative water content (84.0%), leaf water potential (39.73-MPa), leaf area index (36.53 cm²), biological yield (15.10 kgha⁻¹), grain yield (14.40 kgha⁻¹), harvest index (57.70%), catalase (1.54 mmolg⁻¹FW⁻¹), peroxidase (1.90 g⁻¹FWmin⁻¹), and superoxide dismutase (52.60 µgFW⁻¹) were superior under T4 treatment. Soil plant analysis development (54.13 µgcm⁻²) value was also higher under T4 treatment and lowest under T7 treatment. In conclusion, NA-treated plants were more successful in maintaining growth attributes than non-treated plants; therefore, the NA foliar treatment at the rate of 2.2159 gL⁻¹ is suggested to find economical crop yield under drought conditions. The present study would contribute significantly to improving the drought tolerance potential of barley through exogenous NA supply in water deficit areas. Full article

Aluminum antimonide (AlSb), a semiconductor compound, finds its applications in optoelectronics because of its tunable bandgap and promising properties achieved by tailoring suitable dopants. To explore the effects of doping, thin films of pure AlSb and 10% magnesium (Mg)-doped aluminum antimonide (MgAlSb) were synthesized through compound solution deposition on a glass substrate using a low-cost chemical bath deposition (CBD) technique at varying bath temperatures with deposition time intervals ranging from 60 to 180 min. Optical microscopy was used to evaluate the surface roughness and morphology of the synthesized films, revealing the surface roughness and thin film uniformity at different deposition times. The structural characteristics of AlSb and Mg-doped AlSb thin films were further examined using the X-ray diffraction technique, which validated the formation of AlSb and Mg-doped AlSb thin films. This research enables large-scale low-temperature deposition for a variety of conceivable applications in the coatings, materials penetration, energy, and photonic sectors due to the novel properties of this material. Full article

Nonthermal plasma processing is a dry, environment-friendly and chemical-free method of improving the wettability, adhesion, self-cleaning and dying quality of fabrics without affecting their bulk properties. This study presents a green synthesis and coating method for the immobilization of nanoparticles of ZnO on the nonthermal plasma functionalized cotton fabric. The self-cleaning activity of ZnO-coated cotton was then optimized statistically. The ultraviolet protection and antimicrobial activity of the optimized and a control sample were also elaborated in this study. Psidium guajava Linn (guava) plant extract and zinc chloride were used in the ultrasonic biosynthesis of ZnO nanoparticles and concurrent immobilization over plasma functionalized cotton. Sodium hydroxide was used as a reaction accelerator. Statistical complete composite design (CCD) based on the amount of ZnCl₂, NaOH and plasma exposure time was used to optimize the role of input parameters on the self-cleaning ability of the coated cotton. Methylene blue in water was used as a sample pollutant in the self-cleaning study. The ZnO-coated cotton showed notably high self-cleaning activity of 94% and a UV protection factor of 69.87. The antimicrobial activity against E. Coli and S. Aureus bacteria was also appreciably high compared to the control. Full article

Formaldehyde is a primary chemical in the manufacturing of various consumer products. It is synthesized via partial oxidation of methanol using a mixed oxide iron molybdate catalyst (Fe₂(MoO₄)₃–MoO₃). This is one of the standard energy-efficient processes. The mixed oxide iron molybdate catalyst is an attractive commercial catalyst for converting methanol to formaldehyde. However, a detailed phase analysis of each oxide phase and a complete understanding of the catalyst formulation and deactivation studies is required. It is crucial to correctly formulate each oxide phase and influence the synthesis methods precisely. A better tradeoff between support and catalyst and oxygen revival on the catalyst surface is vital to enhance the catalyst’s selectivity, stability, and lifetime. This review presents recent advances on iron molybdate’s catalytic behaviour for formaldehyde production—a deep recognition of the catalyst and its critical role in the processes are highlighted. Finally, the conclusion and prospects are presented at the end. Full article

The power quality of the Electrical Power System (EPS) is greatly affected by electrical harmonics. Hence, accurate and proper estimation of electrical harmonics is essential to design appropriate filters for mitigation of harmonics and their associated effects on the power quality of EPS. This paper presents a novel statistical (Least Square) and meta-heuristic (Grey wolf optimizer) based hybrid technique for accurate detection and estimation of electrical harmonics with minimum computational time. The non-linear part (phase and frequency) of harmonics is estimated using GWO, while the linear part (amplitude) is estimated using the LS method. Furthermore, harmonics having transients are also estimated using proposed harmonic estimators. The effectiveness of the proposed harmonic estimator is evaluated using various case studies. Comparing the proposed approach with other harmonic estimation techniques demonstrates that it has a minimum mean square error with less complexity and better computational efficiency. Full article

The phenotypic and genotypic characteristics of a live-attenuated genotype I (GI) strain (SD12-F120) of Japanese encephalitis virus (JEV) were compared with its virulent parental SD12 strain to gain an insight into the genetic changes acquired during the attenuation process. SD12-F120 formed smaller plaque on BHK-21 cells and showed reduced replication in mouse brains compared with SD12. Mice inoculated with SD12-F120 via either intraperitoneal or intracerebral route showed no clinical symptoms, indicating a highly attenuated phenotype in terms of both neuroinvasiveness and neurovirulence. SD12-F120 harbored 29 nucleotide variations compared with SD12, of which 20 were considered silent nucleotide mutations, while nine resulted in eight amino acid substitutions. Comparison of the amino acid variations of SD12-F120 vs. SD12 pair with those from other four isogenic pairs of the attenuated and their virulent parental strains revealed that the variations at E¹³⁸ and E¹⁷⁶ positions of E protein were identified in four and three pairs, respectively, while the remaining amino acid variations were almost unique to their respective strain pairs. These observations suggest that the genetic changes acquired during the attenuation process were likely to be strain-specific and that the mechanisms associated with JEV attenuation/virulence are complicated. Full article