Search Results (821)

As dengue is an increasing global health threat, a better understanding of the global circulation dynamics and its determinants would be helpful for precise prevention and control of dengue. The dynamics of global circulation of the four dengue virus serotypes were explored utilizing genetic sequences through a network-based method. Four new circulation indicators, including local intensity, betweenness centrality, tip frequency, and persistence time, were defined. Three circulation roles, including source, hub, and destination, were proposed on the basis of new indicators. Spatial and temporal changes of the three circulation roles, along with the persistence time, were explored. Important determinants were also evaluated by machine learning models. Thailand, Indonesia, and Vietnam in Asia and Venezuela and Colombia in Americas were the sources for all four serotypes in different decades. Destinations were observed mostly in island regions. Over the decades, the number of regions with different circulation roles and persistence of DENV-1 increased significantly. Climate and airline factors were involved in the important determinants to circulation roles and persistence of dengue. The roles identified in the global circulation of dengue and important determinants, including climate and airline factors, provide new insights into global dynamics and are beneficial for controlling dengue. Full article

The countries and regions along the Belt and Road are rich in wetland carbon sink resources, crucial for mitigating greenhouse gas emissions and achieving global emission reduction. This paper uses policy analysis and desk research to analyze the overview of wetland carbon sinks in these countries. It explores the necessity of legal system construction for their carbon sink trading. This study finds that smooth trading requires clear property rights definition rules, efficient market trading entities, definite carbon sink trading price rules, financial support aligned with the Equator Principles, and support from biodiversity-compatible environmental regulatory principles. Currently, there are still obstacles in wetland carbon sink trading in the Belt and Road, such as property rights confirmation, an accounting system, an imperfect market trading mechanism, and the coexistence of multiple trading risks. Therefore, this paper first proposes to clarify the goal of the legal guarantee mechanism. Efforts should focus on promoting a consensus on wetland carbon sink ownership and establishing a unified accounting standard system; simultaneously, the relevant departments should conduct field investigations and monitoring, standardize the market order, and strengthen government financial support and funding guarantees. Full article

Background: The short strand-related sequence (SRS) gene family is a class of plant-specific transcription factors related to a group of genes known as the short internode (SHI) or SRS/STY gene family, which plays important roles in regulating plant growth and development and stress responses. Although the SRS genes have been studied in many plants, in cucurbit crops, they have thus far only been identified in cucumber. Methods: In the Cucurbitaceae database from melon (Cucumis melo), cucumber (Cucumis sativus), watermelon (Citrullus lanatus), bottle gourd (Lagenaria siceraria), wax gourd (Benincasa hispida), moschata pumpkin (Cucurbita moschata), and pumpkin (Cucurbita maxima), a total of 60 SRS genes were identified in seven Cucurbitaceae crops, which were classified into three subfamilies. Results: The same subfamily showed conserved motifs and gene structures. The differences in the number of SRS genes in different Cucurbitaceae crops implied likely gene loss or duplication events during evolution. Analysis of promoter cis-regulatory elements indicated that these SRS genes may be involved in hormone response, growth and development, and biotic and abiotic stress responses in plants. Most of the CmSRS genes in melons were expressed in the roots, with a few expressed in the leaves and ovaries. In addition, CmSRS expression was induced by biotic (wilt and powdery mildew) and abiotic (drought and salt) stresses. Subcellular localization of CmSRS proteins showed predominant expression in the nucleus. Conclusions: A total of 60 Cucurbitaceae SRS genes are present in the genomes of seven Cucurbitaceae crops. These cucurbit SRS genes seem to have maintained similar characteristics and functions during the evolutionary process. These results lay the foundation for the study of biological functions of SRS genes in Cucurbitaceae crops. Full article

The thermal environment problem is one of the main focuses of current urban environment research. At present, there are various methods used in urban space thermal environment (USTE) research. As a simulation method to quantify the USTE, the urban weather generator (UWG) has undergone great development and achieved many progressive results. It is necessary to establish and review its current research status by synthesizing UWG multi-scale applications. This review adopts a literature review approach, leveraging the Web of Science Core Collection to obtain previous relevant publications from 2010 to 2025 using “urban weather generator” and “thermal environment” as keywords. The literature is categorized by research themes, including model development, parameter optimization, and application cases. Through innovative analyses of spatio-temporal-scale classification, parameter optimization, the integration of anthropogenic heat emissions, and the multi-domain simulation potential of the UWG, this review synthesizes the application outcomes of the UWG model in multi-scale research, addressing gaps in current urban climate studies. The paper aims to elaborate and analyze the model’s current research status considering the following six aspects. First, the basic parameters in UWG simulation are introduced, including the data and parameter determination settings used in such simulations. Secondly, we introduce the simulation model and its basic principles, the simulation process, and the main steps of this process. Third, we classify and define UWG simulations of spatial thermal environments at different time scales and spatial scales. Fourth, regarding how to improve the accuracy of the UWG model, the deterministic parameters and uncertainty parameters settings are analyzed, respectively. Then, the impacts of anthropogenic heat during the simulation process are also discussed. Fifth, the applications of the UWG model in some major fields and its possible future development directions are addressed. Finally, the existing problems are summarized, the future development trends are prospected, and research on possible expected mitigation measures for the USTE is described. Full article

Liver injury caused by the irrational use of acetaminophen (APAP) represents a significant challenge in the field of public health. In clinical treatment, apart from N—acetylcysteine (NAC), the only approved antidote, there are extremely limited effective intervention measures for APAP-induced hepatotoxicity. Therefore, exploring novel liver-protecting drugs and elucidating their mechanisms of action is of great scientific significance and clinical value. Rosmarinic acid (RA), as a natural polyphenolic compound, has been proven to have significant antioxidant activity. Previous studies have shown that it has a protective effect against drug-induced liver injury. Nevertheless, the precise protective mechanism of RA in APAP-induced acute liver injury (AILI) has not been fully defined. This study was based on an AILI mouse model to systematically explore the liver-protecting effect of RA and its underlying molecular mechanisms. The research results showed that pretreatment with RA could notably mitigate liver pathological injury. It could decrease the activities of ALT and AST in the serum, suppress the liver inflammatory reaction, and reverse the decline in the levels of CAT, T-AOC, SOD, and GSH caused by APAP. Meanwhile, RA could enhance antioxidant defense capabilities by activating the Keap1/Nrf2/HO-1 signaling pathway, regulate the xCT/GPX4 axis to inhibit lipid peroxidation, and thus block the process of ferroptosis. In conclusion, this study confirmed that RA exerts a protective effect against AILI by regulating the Keap1/Nrf2/HO-1 axis to enhance antioxidant capacity and inhibit ferroptosis through the xCT/GPX4 pathway. Our research provides a theoretical basis for RA as a potential therapeutic agent for APAP-induced liver injury. Full article

The desert locust (Schistocerca gregaria) represents one of the most destructive agricultural pests globally, renowned for its ability to form massive swarms that can devastate crops and threaten food security across vast regions. Despite the widespread application of the CRISPR/Cas9 gene-editing system in several insect orders, its utilization in locusts, particularly in the desert locust, has remained relatively unexplored. We established a CRISPR/Cas9-mediated gene-editing workflow for the desert locust using gene encoding for neuropeptide corazonin (Crz) as a target. We also analyzed the phenotypic and physiological characteristics of the mutant using paraffin sectioning, HE staining, and chitin staining techniques. Our findings revealed that while Crz knockout desert locusts were viable and maintained normal fertility, they exhibited striking phenotypic alterations, including albinism and a significant reduction in cuticle thickness. These observations not only highlight the functional role of Crz in pigmentation and cuticle development but also underscore the potential of CRISPR/Cas9 as a powerful tool for dissecting gene function in locusts. Furthermore, the successful application of CRISPR/Cas9 in desert locusts also paves the way for similar genetic studies in other non-model insects, expanding the scope of functional genomics in entomology. Full article

Information regarding the impact of polymetallic exposure on metabolic syndrome (MetS) among residents living near abandoned Pb-Zn mines is limited. Our objective was to investigate the impact of co-exposure to metal mixtures on the prevalence of MetS among residents. ICP-MS was used to measure the levels of 24 metals in the urine of 1744 participants, including 723 participants living near abandoned Pb-Zn mines, labeled as exposed area, and 1021 participants from other towns, labeled as reference area in the same city. Multivariable generalized linear regression, adaptive LASSO penalized regression, and BKMR were used to assess the associations between metals and MetS. The levels of eleven metals were higher, while those of nine metals were lower in the exposed area than those in the reference area. Mg, Cd, Ti, TI, Zn, Rb, and Pb were selected as important MetS predictors using LASSO regression. In exposed area, urinary Zn and TI were positively associated with MetS, whereas Mg was negatively associated with MetS. In the reference area, urinary Zn was positively associated with MetS, whereas Mg and Ti were negatively associated with MetS. The BKMR model indicates a statistically significant positive overall effect of the seven metal mixtures on MetS in the exposed area. Polymetallic exposure was positively associated with MetS risk in the abandoned Pb-Zn mining areas, suggesting that excessive Zn and TI may be associated with a higher MetS risk among residents living near abandoned Pb-Zn mines. Full article

Characterized by insufficient blood supply leading to tissue hypoxia and damage, ischemia is the underlying cause of major conditions such as ischemic stroke, myocardial infarction, and peripheral artery disease. Stem cell therapy, as a regenerative strategy, demonstrates significant potential in restoring tissue blood flow and organ function in ischemic environments. This review systematically explores the latest advances in stem cell therapy for ischemic diseases, focusing on different cell types and their mechanisms of action, including direct differentiation, paracrine signaling, immunomodulation, and microenvironment regulation. Furthermore, it highlights innovations in gene editing and bioengineering technologies that enhance cell delivery, targeting, and therapeutic efficacy. Simultaneously, this article discusses the challenges faced, advances in cell tracking and delivery, and future research directions, aiming to provide insights for the development of more effective and personalized treatment strategies Full article

Autoimmune hepatitis (AIH) is linked to an increased risk of hepatocellular carcinoma (HCC). However, the precise connection between the two remains unclear. GPR81, a G-protein-coupled receptor located on the membranes of various cell types, plays a role in numerous physiological processes. We established an AIH animal model and activated GPR81 using the agonist 3,5-dihydroxybenzoic acid (3,5-DHBA). Additionally, the effect of GPR81 inhibition on tumor and immune cell dynamics was examined using the HepG2, Hep3B, and Hepa1-6 cell lines with the antagonist 3-hydroxybutyric acid (3-OBA). Our results demonstrated that 3,5-DHBA treatment reduced T cell and pro-inflammatory cytokine secretion, while MDSC secretion increased, inhibiting Concanavalin A (Con A)-induced AIH. The inhibition of GPR81 by 3-OBA suppressed HCC cell proliferation and invasion, reduced tumor volume and weight, and downregulated PD-L1 expression. Furthermore, CTL and DC activity in the spleen and tumors increased, while MDSC activity decreased. This study confirms that GPR81 plays an important role in both inflammation and tumorigenesis, suggesting that GPR81 may serve as a bridge in the transformation of inflammation into cancer. Modulating GPR81 activity may provide a novel therapeutic strategy for hepatitis and cancer. Full article

Crop straw chopping and returning technology has gained global implementation to enhance soil structure and fertility, facilitating increased crop yield. Nevertheless, technological adoption faces challenges from inherent limitations in machinery performance, including poor chopping and returning quality and high energy consumption. Consequently, this review first presented a theoretical framework that described the mechanical properties of straw, its fracture dynamics, interactions with airflow, and motion characteristics during the chopping process. Then, based on the straw returning process, the chopping devices were classified into five types: the chopped blade, the chopping machine, the chopping device combined with a no-tillage or reduced-tillage seeder, the chopping and ditch-burying machine, the chopping and mixing machine, and the harvester-powered chopping device. Advancements in spreading devices were also summarized. Finally, six key directions for future research were proposed: developing an intelligent field straw distribution mapping system, engineering adaptive self-regulating mechanisms for chopping and returning equipment, elucidating the mechanics and kinematics of straw in the chopping and returning process, implementing real-time quality assessment systems for straw returning operations, pioneering high forward-speed (>8 km/h) straw returning machines, and establishing context-specific straw residue management frameworks. This review provided a reference and offered support for the global application of straw returning technology. Full article

Against the backdrop of the widespread integration of Artificial Intelligence (AI) into educational practices, collaboration between teachers and AI is profoundly influencing teaching behavior. Drawing on the Conservation of Resources Theory, this study constructs and tests a model examining the impact of teacher-AI collaboration on teaching engagement, with a focus on the mediating role of technological self-efficacy and the moderating role of perceived organizational support. Based on empirical data collected through a survey in China, the results reveal that teacher-AI collaboration significantly and positively predicts teaching engagement. Furthermore, technological self-efficacy mediates this relationship, suggesting that AI collaboration enhances teaching engagement by boosting teachers’ confidence in using technology. In addition, perceived organizational support positively moderates the effect of teacher-AI collaboration on technological self-efficacy, forming a moderated mediation model. This research enriches the understanding of teacher behavior in the context of AI integration and offers practical implications for educational institutions seeking to optimize AI adoption strategies and enhance teacher motivation. Full article

Atmospheric pressure gradients determine the dynamics of the southwest monsoon (SWM) and northeast monsoon (NEM), resulting in rainfall in the Indian subcontinent. Consequently, the surface salinity, mixed layer, and thermocline are impacted by the seasonal freshwater outflow and direct rainfall. Moreover, seasonally reversing monsoon gyre and associated currents govern the northern Indian Ocean surface oceanography. This study provides an overview of the impact of these dynamic changes on sea surface temperature, salinity, and productivity by integrating more than 3000 planktonic foraminiferal censuses and bulk sediment geochemical data from sediment core tops, plankton tows, and nets between 25° N and 10° S and 40° E and 110° E of the past six decades. These data were used to construct spatial maps of the five most dominant planktonic foraminifers and illuminate their underlying environmental factors. Moreover, the cured foraminiferal censuses and the modern oceanographic data were used to test the newly developed artificial neural network (ANN) algorithm to calculate the relationship with modern water column temperatures (WCTs). Furthermore, the tested relationship between the ANN derived models was applied to two foraminiferal censuses from the northern Bay of Bengal core MGS29-GC02 (13°31′59″ N; 91°48′21″ E) and the southern Bay of Bengal Ocean Drilling Program (ODP) Site 758 (5°23.05′ N; 90°21.67′ E) to reconstruct the WCTs of the past 890 ka. The reconstructed WCTs at the 10 m water depth of core GC02 suggest dramatic changes in the sea surface during the deglacial periods (i.e., Bolling–Allerǿd and Younger Dryas) compared to the Holocene. The WCTs at Site 758 indicate a shift in the mixed-layer summer temperature during the past 890 ka at the ODP Site, in which the post-Mid-Brunhes period (at 425 ka) was overall warmer than during the prior time. However, the regional alkenone-derived sea-surface temperatures (SSTs) do not show such a shift in the mixed layer. Therefore, this study hypothesizes that the divergence in regional SSTs is most likely due to differences in seasonality and depth habitats in the paleo-proxies. Full article

Nickel-based superalloys, renowned for their exceptional high-temperature strength, oxidation resistance, and corrosion resistance, have become essential materials in the aerospace, defense, and nuclear industries. However, due to their poor machinability, common cutting processes often result in poor surface quality, difficulties in chip breaking, and significant tool wear. This study investigates the surface integrity of nickel-based superalloys during ultrasonic elliptical vibration cutting. The effects of various process parameters on the surface roughness, residual stress, and microhardness are systematically analyzed. The results indicate that under ultrasonic elliptical vibration cutting conditions, the surface roughness of the workpiece increases with the ultrasonic amplitude, cutting depth, and feed rate. It initially decreases and then increases with cutting speed, and decreases with an increase in the tool tip radius. The post-cutting residual stress in the nickel-based superalloy decreases with higher cutting speed and ultrasonic amplitude, but increases with greater cutting depth and tool tip radius. The surface microhardness increases with the cutting speed up to a point, after which it decreases, while it significantly increases with a higher ultrasonic amplitude, feed rate, and cutting depth. A comparative experiment was conducted between ultrasonic elliptical vibration and conventional cutting. The research results showed that when the cutting depth was 2 µm, the surface roughness and wear decreased by 19% and 53%, respectively, and the residual compressive stress and microhardness increased by 44% and 21%, respectively. This further verified the significant advantages of ultrasonic elliptical vibration cutting in optimizing machining performance. Full article

Ophiocordyceps sinensis, is an entomopathogenic fungus renowned for its medicinal properties, thriving in the frigid and high-altitude regions of the Qinghai–Tibet plateau. Given the limited availability of wild resources and the increasing recognition of their medicinal value, the cultivation of O. sinensis was initiated. However, there is a paucity of research investigating the disparities in their quality. This study evaluated the primary physiological indicators of both wild and cultivated O. sinensis. It also employed proteome and untargeted metabolome approaches to elucidate the differences in quality and underlying mechanisms between the two types. The results revealed that the contents of key representative components, including polysaccharide, crude protein, adenosine, and mannitol, were higher in wild O. sinensis than in cultivated O. sinensis. A total of 499 differentially expressed proteins (DEPs), including 117 up-regulated and 382 down-regulated DEPs, were identified in wild and cultivated O. sinensis. Additionally, 369 up-regulated differentially accumulated metabolites (DAMs) and 737 down-regulated DAMs were also identified. Wild O. sinensis had higher relative levels of lysophospholipid metabolites, while cultivated O. sinensis had higher relative levels of aldehydes and carboxylic acids. Correlation analysis revealed that different habitats altered 47 pathways shared between the proteome and metabolome, including carbohydrate metabolism and energy metabolism. β-glucosidase and α-galactosidase play essential roles in carbohydrate catabolism and may indirectly influence amino acid synthesis through energy metabolic pathways. The differential expression of polyamine oxidase (PAO) could reflect variations in polyamine metabolism and ammonia production between wild and cultivated O. sinensis. These variations may consequently affect nitrogen homeostasis and the biosynthesis of nitrogen-containing compounds, ultimately leading to differences in nutritional quality. In conclusion, these findings offer a novel perspective on the applications of O. sinensis and serve as a reference for the targeted development of cultivated O. sinensis. Full article