Search Results (13)

Structural muscle changes, including muscle atrophy and fatty infiltration, follow rotator cuff tendon tear and are associated with a high repair failure rate. Despite extensive research efforts, no pharmacological therapy is available to successfully prevent both muscle atrophy and fatty infiltration after tenotomy of tendomuscular unit without surgical repair. Poly(ADP-ribose) polymerases (PARPs) are identified as a key transcription factors involved in the maintenance of cellular homeostasis. PARP inhibitors have been shown to influence muscle degeneration, including mitochondrial hemostasis, oxidative stress, inflammation and metabolic activity, and reduced degenerative changes in a knockout mouse model. Tenotomized infraspinatus were assessed for muscle degeneration for 16 weeks using a Swiss Alpine sheep model (n = 6). All sheep received daily oral administration of 0.5 mg Talazoparib. Due to animal ethics, the treatment group was compared with three different controls from prior studies of our institution. To mitigate potential batch heterogeneity, PARP-I was evaluated in comparison with three distinct control groups (n = 6 per control group) using the same protocol without treatment. The control sheep were treated with an identical study protocol without Talazoparib treatment. Muscle atrophy and fatty infiltration were evaluated at 0, 6 and 16 weeks post-tenotomy using DIXON-MRI. The controls and PARP-I showed a significant (control p < 0.001, PARP-I p = 0.01) decrease in muscle volume after 6 weeks. However, significantly less (p = 0.01) atrophy was observed in PARP-I after 6 weeks (control 1: 76.6 ± 8.7%; control 2: 80.3 ± 9.3%, control 3: 73.8 ± 6.7% vs. PARP-I: 90.8 ± 5.1% of the original volume) and 16 weeks (control 1: 75.7 ± 9.9; control 2: 74.2 ± 5.6%; control 3: 75.3 ± 7.4% vs. PARP-I 93.3 ± 10.6% of the original volume). All experimental groups exhibited a statistically significant (p < 0.001) augmentation in fatty infiltration following a 16-week period when compared to the initial timepoint. However, the PARP-I showed significantly less fatty infiltration (p < 0.003) compared to all controls (control 1: 55.6 ± 6.7%, control 2: 53.4 ± 9.4%, control 3: 52.0 ± 12.8% vs. PARP-I: 33.5 ± 8.4%). Finally, a significantly (p < 0.04) higher proportion and size of fast myosin heavy chain-II fiber type was observed in the treatment group. This study shows that PARP-inhibition with Talazoparib inhibits the progression of both muscle atrophy and fatty infiltration over 16 weeks in retracted sheep musculotendinous units. Full article

Background: Compared to conventional 2D mammography, digital breast tomosynthesis (DBT) offers greater breast lesion detection rates. Ring-like hypodense artifacts surrounding dense lesions are a common byproduct of DBT. This study’s purpose was to assess whether minuscule changes spanning this halo—termed the “broken halo sign”—could improve lesion classification. Methods: This retrospective study was approved by the local ethics review board. After screening 288 consecutive patients, DBT studies of 191 female participants referred for routine mammography with a subsequent histologically verified finding of the breast were assessed. Examined variables included patient age, histological diagnosis, architectural distortion, maximum size, maximum halo depth, conspicuous margins, irregular shape and broken halo sign. Results: While a higher halo strength was indicative of malignancy in general (p = 0.031), the broken halo sign was strongly associated with malignancy (p < 0.0001, odds ratio (OR) 6.33), alongside architectural distortion (p = 0.012, OR 3.49) and a diffuse margin (p = 0.006, OR 5.49). This was especially true for denser breasts (ACR C/D), where the broken halo sign was the only factor predicting malignancy (p = 0.03, 5.22 OR). Conclusion: DBT-associated halo artifacts warrant thorough investigation in newly found breast lesions as they are associated with malignant tumors. The “broken halo sign”—the presence of small lines of variable diameter spanning the peritumoral areas of hypodensity—is a strong indicator of malignancy, especially in dense breasts, where architectural distortion may be obfuscated due to the surrounding tissue. Full article

Admired and despised, created and destroyed, legal and illegal: Contemporary graffiti are polarising, and not everybody agrees to label them as cultural heritage. However, if one is among the steadily increasing number of heritage professionals and academics that value these short-lived creations, their digital documentation can be considered a part of our legacy to future generations. To document the geometric and spectral properties of a graffito, digital photographs seem to be appropriate. This also holds true when documenting an entire graffiti-scape consisting of 1000s of individual creations. However, proper photo-based digital documentation of such an entire scene comes with logistical and technical challenges, certainly if the documentation is considered the basis for further analysis of the heritage assets. One main technical challenge relates to the photographs themselves. Conventional photographs suffer from multiple image distortions and usually lack a uniform scale, which hinders the derivation of dimensions and proportions. In addition, a single graffito photograph often does not reflect the meaning and setting intended by the graffitist, as the creation is frequently shown as an isolated entity without its surrounding environment. In other words, single photographs lack the spatio-temporal context, which is often of major importance in cultural heritage studies. Here, we present AUTOGRAF, an automated and freely-available orthorectification tool which converts conventional graffiti photos into high-resolution, distortion-free, and georeferenced graffiti orthophotomaps, a metric yet visual product. AUTOGRAF was developed in the framework of INDIGO, a graffiti-centred research project. Not only do these georeferenced photos support proper analysis, but they also set the basis for placing the graffiti in their native, albeit virtual, 3D environment. An experiment showed that 95 out of 100 tested graffiti photo sets were successfully orthorectified, highlighting the proposed methodology’s potential to improve and automate one part of contemporary graffiti’s digital preservation. Full article

Since tissue material is often lacking in metastatic prostate cancer (mPCa), there is increasing interest in using liquid biopsies for treatment decision and monitoring therapy responses. The purpose of this study was to validate the usefulness of circulating tumor cells (CTCs) and plasma-derived cell-free (cf) RNA as starting material for gene expression analysis through qPCR. CTCs were identified upon prostate-specific membrane antigen and/or cytokeratin positivity after enrichment with ScreenCell (Westford, Massachusetts, USA) filters or the microfluidic Parsortix^TM (Guildford, Surrey, United Kingdom) system. Overall, 50% (28/56) of the patients had ≥5 CTCs/7.5 mL of blood. However, CTC count did not correlate with Gleason score, serum PSA, or gene expression. Notably, we observed high expression of CD45 in CTC samples after enrichment, which could be successfully eliminated through picking of single cells. Gene expression in picked CTCs was, however, rather low. In cfRNA from plasma, on the other hand, gene expression levels were higher compared to those found in CTCs. Moreover, we found that PSA was significantly increased in plasma-derived cfRNA of mPCa patients compared to healthy controls. High PSA expression was also associated with poor overall survival, indicating that using cfRNA from plasma could be used as a valuable tool for molecular expression analysis. Full article

In order to meet the technical specifications in roofing applications, the bitumen used for this purpose is standardly modified by polymers. This, in general, allows the re-use of recycled polymer during the production of polymer-modified bitumen (PmB), simultaneously reducing the amount of polymeric waste. Recycling processes, however, may degrade or contaminate polymers, leading to reduced crystallinity and lower melting temperature. Six different recycled polyolefins (high crystallinity: iPP, HDPE; reduced crystallinity: APP, PP Copolymer; waxy polyolefins: Wax 105, Wax 115) were assessed on their suitability for roofing applications. Mixing characteristics, polymer distribution and thermo-mechanical properties of the PmB samples were determined, employing fluorescence microscopy, modulated temperature differential scanning calorimetry (MTDSC) and dynamic shear rheometry (DSR). Depending on mixing properties, two levels of polymer content (5 and 16 wt% or 16 and 30 wt%) were considered. High crystallinity polymers exhibited the biggest increase in |G*| and lowest phase angle. Reduced crystallinity polymers were more easily dispersed and showed improved |G*| and phase angle. Waxy polyolefins improved bitumen similarly to reduced crystallinity polymers and are easily dispersed. The results suggest, that a reduced crystallinity or lower melting temperature of the recycled polymers resulting from degradation or contamination may be beneficial, resulting in improved mixing behavior and a more homogeneous distribution of the polymer within the bitumen. Full article

The aim of this work is to determine the influence of the bitumen chemistry on the rheological performance of bitumen and polymer modified bitumen (PmB), as well as the polymer distribution and storage stability. Six different bitumens and their 5 wt.% SBS mixtures are considered in this work. The bitumen composition was determined by SARA fractioning, which was then correlated with the glass transition temperature, complex modulus |G*|, and phase angle, which were obtained by parallel-plate dynamic shear rheology in the temperature range of −25 to 65 °C. The polymer distribution, which was derived from fluorescence microscopy images and the storage stability (determined by tube test) also correlated with the SARA fractions. It was found that the saturates decrease |G*| and T${}_g$ and increase the phase angle in crude bitumen, while the asphaltenes increase |G*| and the phase angle. For PmB, the amount of swelling was determined by the saturate content of bitumen. The glass transition temperature of PmBs increases for low saturate and decreases for high saturate contents. |G*| and the phase angle of PmBs correlates with the saturate content, with a varying influence depending on a high or low saturate content and the temperature range due to saturate depletion in the bitumen-rich phase and the varying vol% polymer-rich phase. The aromatic and resin fractions show no correlation in the considered bitumens and PmBs. Full article

The measurements of tree attributes required for forest monitoring and management planning, e.g., National Forest Inventories, are derived by rather time-consuming field measurements on sample plots, using calipers and measurement tapes. Therefore, forest managers and researchers are looking for alternative methods. Currently, terrestrial laser scanning (TLS) is the remote sensing method that provides the most accurate point clouds at the plot-level to derive these attributes from. However, the demand for even more efficient and effective solutions triggers further developments to lower the acquisition time, costs, and the expertise needed to acquire and process 3D point clouds, while maintaining the quality of extracted tree parameters. In this context, photogrammetry is considered a potential solution. Despite a variety of studies, much uncertainty still exists about the quality of photogrammetry-based methods for deriving plot-level forest attributes in natural forests. Therefore, the overall goal of this study is to evaluate the competitiveness of terrestrial photogrammetry based on structure from motion (SfM) and dense image matching for deriving tree positions, diameters at breast height (DBHs), and stem curves of forest plots by means of a consumer grade camera. We define an image capture method and we assess the accuracy of the photogrammetric results on four forest plots located in Austria and Slovakia, two in each country, selected to cover a wide range of conditions such as terrain slope, undergrowth vegetation, and tree density, age, and species. For each forest plot, the reference data of the forest parameters were obtained by conducting field surveys and TLS measurements almost simultaneously with the photogrammetric acquisitions. The TLS data were also used to estimate the accuracy of the photogrammetric ground height, which is a necessary product to derive DBHs and tree heights. For each plot, we automatically derived tree counts, tree positions, DBHs, and part of the stem curve from both TLS and SfM using a software developed at TU Wien (Forest Analysis and Inventory Tool, FAIT), and the results were compared. The images were oriented with errors of a few millimetres only, according to checkpoint residuals. The automatic tree detection rate for the SfM reconstruction ranges between 65% and 98%, where the missing trees have average DBHs of less than 12 cm. For each plot, the mean error of SfM and TLS DBH estimates is −1.13 cm and −0.77 cm with respect to the caliper measurements. The resulting stem curves show that the mean differences between SfM and TLS stem diameters is at maximum −2.45 cm up to 3 m above ground, which increases to almost +4 cm for higher elevations. This study shows that with the adopted image capture method, terrestrial SfM photogrammetry, is an accurate solution to support forest inventory for estimating the number of trees and their location, the DBHs and stem curve up to 3 m above ground. Full article

Airborne laser scanning (ALS) data can provide more than just a topographic data set for archaeological research. During data collection, laser scanning systems also record radiometric information containing object properties, and thus information about archaeological features. Being aware of the physical model of ALS scanning, the radiometric information can be used to calculate material information of the scanned object. The reflectance of an object or material states the amount of energy it reflects for a specific electromagnetic wavelength. However, the collected radiometric data are affected by several factors that cause dissimilar values to be recorded for the same object. Radiometric calibration of such data minimizes these differences in calculated reflectance values of objects, improving their usability for feature detection and visualization purposes. Previous work dealing with calibration of radiometric data in archaeological research has relied on corresponding in-field measurements to acquire calibration values or has only corrected for a limited number of variables. In this paper, we apply a desk-based approach in which radiometric calibration is conducted through the selection of homogenous areas of interest, without the use of in-field measurements. Together with flight and scan parameters, radiometric calibration allows for the estimation of reflectance values for returns of a single full-waveform ALS data collection flight. The resulting data are then processed into a raster reflectance map that approximates a monochromatic illumination-independent true orthoimage at the wavelength of the laser scanner. We apply this approach to data collected for an archaeological research project in western Sicily and discuss the relative merits of the uses of radiometric data in such locations as well as its wider applicability for present and future archaeological and environmental research. In order to make the approach more accessible, we have developed a freely available tool that allows users to apply the calibration procedure to their own data. Full article

Aluminium coatings and their characterization are of great interest in many fields of application, ranging from aircraft industries to microelectronics. Here, we present the simulation of acoustic wave propagation in aluminium coatings via the elastodynamic finite integration technique (EFIT) in comparison to experimental results. The simulations of intensity (I)–defocus (z) curves, obtained by scanning acoustic microscopy (SAM), were first carried out on an aluminium bulk sample, and secondly on a 1 µm aluminium coating deposited on a silicon substrate. The I(z) curves were used to determine the Rayleigh wave velocity of the aluminium bulk sample and the aluminium coating. The results of the simulations with respect to the Rayleigh velocity were corroborated by non-destructive SAM measurements and laser ultrasonic measurements (LUS). Full article

Diameter at breast height (DBH) is one of the most important parameter in forestry. With increasing use of terrestrial and airborne laser scanning in forestry, new exceeding possibilities to directly derive DBH emerge. In particular, high resolution point clouds from laser scanners on board unmanned aerial systems (UAS) are becoming available over forest areas. In this case study, DBH estimation from a UAS point cloud based on modeling the relevant part of the tree stem with a cylinder, is analyzed with respect to accuracy and completeness. As reference, manually measured DBHs and DBHs from terrestrial laser scanning point clouds are used for comparison. We demonstrate that accuracy and completeness of the cylinder fit are depending on the stem diameter. Stems with DBH > 20 cm feature almost 100% successful reconstruction with relative differences to the reference DBH of 9% (DBH 20–30 cm) down to 1.8% for DBH > 40 cm. Full article

The main purpose of any aerial photo archive is to allow quick access to images based on content and location. Therefore, next to a description of technical parameters and depicted content, georeferencing of every image is of vital importance. This can be done either by identifying the main photographed object (georeferencing of the image content) or by mapping the center point and/or the outline of the image footprint. The paper proposes a new image archiving workflow. The new pipeline is based on the parameters that are logged by a commercial, but cost-effective GNSS/IMU solution and processed with in-house-developed software. Together, these components allow one to automatically geolocate and rectify the (oblique) aerial images (by a simple planar rectification using the exterior orientation parameters) and to retrieve their footprints with reasonable accuracy, which is automatically stored as a vector file. The data of three test flights were used to determine the accuracy of the device, which turned out to be better than 1° for roll and pitch (mean between 0.0 and 0.21 with a standard deviation of 0.17–0.46) and better than 2.5° for yaw angles (mean between 0.0 and −0.14 with a standard deviation of 0.58–0.94). This turned out to be sufficient to enable a fast and almost automatic GIS-based archiving of all of the imagery. Full article

Airborne LiDAR Bathymetry (ALB) has been rapidly evolving in recent years and now allows fluvial topography to be mapped in high resolution (>20 points/m²) and height accuracy (<10 cm) for both the aquatic and the riparian area. This article presents methods for enhanced modeling and monitoring of instream meso- and microhabitats based on multitemporal data acquisition. This is demonstrated for a near natural reach of the Pielach River, with data acquired from April 2013 to October 2014, covering two flood events. In comparison with topographic laser scanning, ALB requires a number of specific processing steps. We present, firstly, a novel approach for modeling the water surface in the case of sparse water surface echoes and, secondly, a strategy for improved filtering and modeling of the Digital Terrain Model of the Watercourse (DTM-W). Based on the multitemporal DTM-W we discuss the massive changes of the fluvial topography exhibiting deposition/erosion of 10³ m³ caused by the 30-years flood event in May 2014. Furthermore, for the first time, such a high-resolution data source is used for monitoring of hydro-morphological units (mesohabitat scale) including the consequences for the target fish species nase (Chondrostoma nasus, microhabitat scale). The flood events caused a spatial displacement of the hydro-morphological units but did not effect their overall frequency distribution, which is considered an important habitat feature as it documents resilience against disturbances. Full article

Delay tracking of spread-spectrum signals is widely used for ranging in radio frequency based navigation. Its use in non-coherent optical ranging, however, has not been extensively studied since optical channels are less subject to narrowband interference situations where these techniques become more useful. In this work, an early-late delay-locked loop adapted to indoor optical ranging is presented and analyzed. The specific constraints of free-space infrared channels in this context substantially differ from those typically considered in radio frequency applications. The tracking stage is part of an infrared differential range measuring system with application to mobile target indoor localization. Spread-spectrum signals are used in this context to provide accurate ranging while reducing the effect of multipath interferences. The performance of the stage regarding noise and dynamic errors is analyzed and validated, providing expressions that allow an adequate selection of the design parameters depending on the expected input signal characteristics. The behavior of the stage in a general multipath scenario is also addressed to estimate the multipath error bounds. The results, evaluated under realistic conditions corresponding to an 870 nm link with 25 MHz chip-rate, built with low-cost up-to-date devices, show that an overall error below 6% of a chip time can be achieved. Full article