Search Results (30)

The development of wheat cultivars capable of withstanding drought conditions is necessary for global food security. Conventional breeding, emphasizing the exploitation of inherent genetic diversity by selecting wheat genotypes exhibiting superior drought-related traits, including root architecture, water use efficiency, and stress-responsive genes, has been used by breeders. Simultaneously, molecular techniques such as marker-assisted selection and gene editing are deployed to accelerate the identification and integration of specific drought-responsive genes into elite wheat lines. Cutting-edge genomic tools play a pivotal role in decoding the genetic basis of wheat drought tolerance, enabling the precise identification of key genomic regions and facilitating breeding decisions. Gene-editing technologies, deployed judiciously, ensure the targeted enhancement of desirable traits without compromising the overall genomic integrity of wheat varieties. This review introduces a strategic amalgamation of conventional and molecular breeding approaches for developing drought-tolerant wheat. The review aims to accelerate progress by seamlessly merging traditional breeding methods with advanced molecular tools, and it also underscores the potential of a synergistic future for enhancing wheat drought resilience, providing a roadmap for the development of resilient wheat varieties essential for sustainable agriculture in the 21st century. Full article

A symptom of reduced feed intake, conception and progressive emaciation was noticed in the Murrah buffalo farm of the institute with tail gangrene in some buffaloes and the sudden death of many animals. Thus, the objective of the study was for the systemic investigation to find out the causative agents and necessary ameliorative measures. The tail lesion includes alopecia, scales, necrosis, oedematous and a painful area. After thorough examination of the signs and symptoms of the disease, it was speculated that the case may be due to the presence of mycotoxins in the feeds offered to the animals. The severely affected buffaloes that died subjected to post-mortem examination demonstrated liver damage, nephritis and haemorrhages in all the vital organs. The analyses of offered feed revealed a high concentration of aflatoxin B1 content in maize, groundnut cake, cottonseed cake and compound feed mixtures. The case was typically diagnosed as the aflatoxicosis in water buffalo and ameliorative measures viz. the withdrawal of contaminated feeds, supplementation of toxin binder and penta-sulphate mixture in the feed taken sustained animal health and production performances. Severely affected animals with tail gangrene were treated with local disinfectants and antibiotics as well as systemic injection with broad-spectrum antibiotics and supportive vitamins and minerals to recover to their previous stage. Therefore, routine check-ups of feeds are of utmost importance to prevent feeding of aflatoxin-contaminated feeds. Systemic efforts viz. therapeutic management with topical medicines, broad-spectrum antibiotics, supportive therapies with vitamins and antioxidants along with replacement of contaminated feeds and inclusion of peta-sulphate mixture, and a toxin binder are effective in the prevention and control of aflatoxicosis in buffaloes. Full article

Worldwide zinc deficiency in the soil under cereal production is a common problem affecting the yield and nutritional value of several crops. Bioaugmentation of soil zinc with zinc-solubilizing bacteria can be a promising option for increasing the zinc nutrition to crops. The objectives of the study were to evaluate Bacillus paramycoides for improving yield, zinc nutrition, and zinc availability in rice grown under sodicity stress caused by alkali water irrigation. Treatments included T₁: control, T₂: substrate, T₃: Bacillus paramycoides, T₄: control (T₁) + zinc sulphate, T₅: substrate (T₂) + zinc sulphate, and T₆: Bacillus paramycoides (T₃) + zinc sulphate. Rice yield, zinc content, and uptake, and apparent zinc recovery were not altered by Bacillus paramycoides. The different fractions of zinc measured after 30 and 60 days after transplanting of the rice remain unaffected by the inoculation of Bacillus paramycoides. Further, an equal number of zinc-solubilizing bacteria present in the rice rhizosphere of control plots after 30 days of transplanting suggests the importance of the native rhizospheric microbiome in zinc nutrition. It is concluded that the application of Bacillus paramycoides in sodicity-stressed rice did not provided additional benefits in terms of zinc nutrition and yield. Further investigation will be required to improve the apparent zinc recovery of crops in those areas, where alkali water is continuously utilized for irrigation. Full article

This paper assesses the performance of waste heat recovery from commercial kitchen wastewater in practice. A pilot study of heat recovery from the kitchen at Penrhyn Castle, a tourist attraction in North Wales (UK), is outlined. The pilot heat recovery site was designed and installed, comprising a heat exchanger, recirculation pumps, buffer tank and an extensive temperature/flow monitoring system for performance monitoring of the waste heat recovery system. Continuous monitoring was conducted for a period of 8 months, covering the 2022 tourist season. The recovered heat from the kitchen wastewater preheats the incoming cold freshwater supply and consequently reduces the amount of energy consumed for subsequent water heating. Retrofitting the pilot heat recovery system to the kitchen drains resulted in a heat saving of 240 kWh per month on average, a reduction of 928.8 kg CO₂e per year, and a payback period for the investment costs of approximately two years, depending on the cost of energy supply. The presented results illustrate the potential of this form of renewable heat in reducing the carbon footprint of water heating activities in buildings and the hospitality sector. Full article

A growing population, changing climate, scarcity of resources, and the urgent need to achieve sustainable development goals makes it imperative to reimagine agriculture in a way that makes it economically profitable, climate-resilient, resource-efficient. Traditional rice production technology involving puddling and transplanting has often been criticized for its inefficient resource utilization, high cost of production, and emission of greenhouse gases such as methane. Direct-seeded rice (DSR), promoted for its climate-resilient nature, is often utilized by farmers in three different ways: broadcasting, line sowing, and zero tillage. However, these establishment methods have certain advantages and limitations, as perceived by farmers. The present study attempts to closely study the crop performance of rice under the abovementioned crop establishment methods. The study was conducted in farmers’ fields in a way where both the farmers’ freedom and research conditions were equally taken care of. The study aims to analyze crop performance while emphasizing farmers’ field-based knowledge to ensure a lab-land-lab loop for understanding the scope of refinement in agronomic as well extension strategies. The results of this study reveal the superiority of zero tillage over broadcasting and line sowing in terms of crop performance and economic performance in the northwest alluvial plain zone of Bihar. The study has also identified the constraints associated with adoption of line sowing and zero tillage. Full article

Slopy agricultural lands are more susceptible to soil erosion and hence are priority sites for the application of protective soil management practices. A conservation agriculture field experiment was established at a 6% field slope in 2011 at the ICAR-IISWC Research Farm, Dehradun, Uttarakhand, which is situated in the Northwestern Himalayan Region, India. The objective of this study was to experimentally determine the long-term effects of tillage practices on runoff and soil erosion. The tillage practices opted for were conventional tillage (CT), minimum tillage (MT), and zero tillage (ZT). Event-based runoff and soil loss were monitored during three monsoon seasons (June to September) from 2018 to 2020. Results showed lower runoff and soil loss in the ZT plot than in CT and MT plots. CT produced 1.51 and 2.53 times higher runoff than MT and ZT, respectively. Moreover, this increased runoff generated 1.84 and 5.10 times higher soil erosion in CT than in MT and ZT, respectively. The extreme rainfall events being less than 10% generated 54.93%, 57.35%, and 63.43% of the total runoff volume which resulted in 82.08%, 85.49%, and 91.00% of the total soil loss in CT, MT, and ZT plots, respectively. For the same amount of rainfall, the reduction in soil loss was 39% and 68% in the CT and ZT plots, respectively, at the highest growth stage in comparison to the initial crop growth stage. The values of runoff reduction benefit (RRB) and sediment reduction benefit (SRB) showed a reduction in runoff (63.53%) and soil loss (80.39%) in the CT. Results concluded that conservation tillage reduced runoff and soil loss significantly even in extreme rainfall events. Full article

Cancer is a complex and multifaceted group of diseases characterized by the uncontrolled growth and spread of abnormal cells. While cancer can be challenging and life-altering, advances in research and development have led to the identification of new promising anti-cancer targets. Telomerase is one such target that is overexpressed in almost all cancer cells and plays a critical role in maintaining telomere length, which is essential for cell proliferation and survival. Inhibiting telomerase activity can lead to telomere shortening and eventual cell death, thus presenting itself as a potential target for cancer therapy. Naturally occurring flavonoids are a class of compounds that have already been shown to possess different biological properties, including the anti-cancer property. They are present in various everyday food sources and richly present in fruits, nuts, soybeans, vegetables, tea, wine, and berries, to name a few. Thus, these flavonoids could inhibit or deactivate telomerase expression in cancer cells by different mechanisms, which include inhibiting the expression of hTERT, mRNA, protein, and nuclear translocation, inhibiting the binding of transcription factors to hTERT promoters, and even telomere shortening. Numerous cell line studies and in vivo experiments have supported this hypothesis, and this development could serve as a vital and innovative therapeutic option for cancer. In this light, we aim to elucidate the role of telomerase as a potential anti-cancer target. Subsequently, we have illustrated that how commonly found natural flavonoids demonstrate their anti-cancer activity via telomerase inactivation in different cancer types, thus proving the potential of these naturally occurring flavonoids as useful therapeutic agents. Full article

To assess the impact of bamboo plantations on soil organic carbon (SOC) under prevailing climatic conditions, increase in temperature and soil amendments, the Roth C model was used. RothC is a promising model for the estimation of SOC changes in different land use systems. In the present study, the RothC model was used to predict the dynamics of SOC in the plantation of seven bamboo species under a usual scenario: increase temperature by 1 °C and 2 °C and farm yard manure (FYM) addition. The result revealed that RothC fairly predicts the SOC. The root mean square error (RMSE) value varied from 0.74 to 3.2 among seven bamboo species while comparing modeled and measured data. The increase in temperature resulted in a decrease in SOC. The decrease in SOC varied from 0.46 to 5.96 per cent as compared to the usual scenario, and the extent of the decrease varied from species to species. Among all species, the application of 9 t ha⁻¹ FYM was found appropriate for maintaining the initial SOC level during the initial stage of bamboo growth. Full article

Piezo channels belong to an important class of cell membrane-bound, Ca²⁺-permeable, mechanosensitive channels consisting of a pore and multiple transmembrane helices. In plants, the functional aspects of Piezo channels have been less studied than other mechanosensitive channels. However, a few studies that have been carried out indicate the involvement of Piezo channels in stress response and developmental processes. In our analysis, we identified a total of three Piezo genes in the Triticum aestivum genome. The phylogenetic analysis revealed the monocot and dicot-specific clustering of Piezo proteins. The gene and protein structure analysis indicated their conserved architecture. The promoter region of each of the three Piezo genes contained light-, growth-and development-, hormone-, and stress-responsive cis-regulatory elements. Moreover, the differential expression of Piezo genes in tissue developmental stages and under abiotic and biotic stress conditions indicated their probable role in plant growth and development and various stresses. The quantitative real-time polymerase chain reaction (qRT-PCR) analysis suggested that TaPiezo1-D might be involved in Ca²⁺ homeostasis. In addition, protein–protein interaction indicated their precise role in glucose, hormone and stress responses. The miRNA interaction analysis further suggested their participation in signaling cascades and biological processes. The present study will extend our understanding about Piezo channels in Ca²⁺ mediated signaling in plants under various stresses and provide a path for the functional validation of TaPiezo genes in future research. Full article

Bacterial blight (BB) is a devastating disease of rice in the tropics of Indian sub-continent, where the presence of Xoo races with varying levels of genetic diversity and virulence renders disease management extremely challenging. In this context, marker-assisted improvement of plant resistance has been proven as one of the most promising approaches for the development of sustainable rice cultivars. The present study demonstrates the marker-assisted introgression of the three BB resistant genes (Xa21 + xa13 + xa5) into the background of HUR 917, a popular aromatic short grain (ASG) rice cultivar in India. The performance of the resulting improved products (near isogenic lines (NILs), HR 23-5-37-83-5, HR 23-5-37-121-10, HR 23-5-37-121-14, HR 23-65-6-191-13, HR 23-65-6-237-2, HR 23-65-6-258-10 and HR 23-65-6-258-21) establishes the utility of marker-assisted selection (MAS) approach for accelerated trait introgression in rice. The MAS-bred lines carrying three introgressed genes showed broad spectrum BB resistance (lesion length, LL of 1.06 ± 1.35 cm to 4.61 ± 0.87 cm). Besides, these improved lines showed the complete product profile of recurrent parent HUR 917 along with the enhanced level of durable BB resistance. The improved introgression lines with durable BB resistance would contribute to sustainable rice production in India, particularly in the Indo-Gangetic plane that has substantial acreage under HUR 917. Full article

The hyperosmolality-gated calcium-permeable channels (OSCA) are pore-forming transmembrane proteins that function as osmosensors during various plant developmental processes and stress responses. In our analysis, through in silico approaches, a total of 42 OSCA genes are identified in the Triticum aestivum genome. A phylogenetic analysis reveals the close clustering of the OSCA proteins of Arabidopsis thaliana, Oryza sativa, and T. aestivum in all the clades, suggesting their origin before the divergence of dicots and monocots. Furthermore, evolutionary analyses suggest the role of segmental and tandem duplication events (Des) and purifying selection pressure in the expansion of the OSCA gene family in T. aestivum. Expression profiling in various tissue developmental stages and under abiotic and biotic stress treatments reveals the probable functioning of OSCA genes in plant development and the stress response in T. aestivum. In addition, protein–protein and protein–chemical interactions reveal that OSCA proteins might play a putative role in Ca²⁺-mediated developmental processes and adaptive responses. The miRNA interaction analysis strengthens the evidence for their functioning in various biological processes and stress-induced signaling cascades. The current study could provide a foundation for the functional characterization of TaOSCA genes in future studies. Full article

Tremendous progress has been made in cancer research over the years, and, as a result, immunotherapy has emerged as an important therapy for the treatment of cancer, either as a stand-alone treatment or in conjunction with other cancer therapies. Immunotherapy has demonstrated encouraging outcomes and offers a viable strategy for not only enhancing the quality of life but also dramatically boosting the overall survival rate of cancer patients. The objective of this systematic review was to assess the efficacy of immunotherapy in the treatment of cancer. Databases such as PubMed and Science Direct were searched from their inception until September 2021, using the following keywords: cancer immunotherapy, cancer recurrence, cancer treatment options, and cancer therapies. The systematic review was conducted in accordance with the PRISMA protocol. There were a total of 599 articles; however, after applying the inclusion and exclusion criteria, the final review ended up with 34 publications. In conclusion, the studies have demonstrated that immunotherapy is a viable alternative treatment option for patients with recurrent or metastatic cancer, since the overall survival rate and progression-free survival rate were shown to be successful. Full article

Peripheral artery disease (PAD) is characterized by impaired blood flow to the lower extremities, resulting in ischemic limb injuries. Individuals with diabetes and PAD typically have more severe ischemic limb injuries and limb amputations, but the mechanisms involved are poorly understood. Previously, we identified BAG3 as a gene within a mouse genetic locus termed limb salvage QTL1 on mouse chromosome 7 that determined the extent of limb necrosis following ischemic injury in C57Bl/6 mice. Whether BAG3 deficiency plays a role in the severe ischemic injury observed in diabetic PAD is not known. In vitro, we found simulated ischemia enhanced BAG3 expression in primary human skeletal muscle cells, whereas BAG3 knockdown increased necroptosis markers and decreased cell viability. In vivo, ischemic skeletal muscles from hind limbs of high-fat diet (HFD)-fed mice showed poor BAG3 expression compared to normal chow diet (NCD)-fed mice, and this was associated with increased limb amputations. BAG3 overexpression in ischemic skeletal muscles from hind limbs of HFD mice rescued limb amputation and improved autophagy, necroptosis, skeletal muscle function and regeneration. Therefore, BAG3 deficiency in ischemic skeletal muscles contributes to the severity of ischemic limb injury in diabetic PAD, likely through autophagy and necroptosis pathways. Full article

The present study aims to design, develop and characterize kNGR (Asn-Gly-Arg) peptide-conjugated lipid–polymer-based nanoparticles for the target-specific delivery of anticancer bioactive(s), i.e., Paclitaxel (PTX). The kNGR-PEG-DSPE conjugate was synthesized and characterized by using spectral analysis. The dual-targeted PLGA–lecithin–PEG core-shell nanoparticles (PLNs-kNGR-NPs) were synthesized using a modified nanoprecipitation process, and their physiological properties were determined. The results support that, compared to other NPs, PLNs-kNGR-NPs are highly cytotoxic, owing to higher apoptosis and intracellular uptake. The significance of rational nanoparticle design for synergistic treatment is shown by the higher tumor volume inhibition percentage rate (59.7%), compared to other designed formulations in Balb/c mice in the HT-1080 tumor-induced model. The overall results indicate that the PLNs-kNGR-NPs-based hybrid lipid–polymer nanoparticles present the highest therapeutic efficacy against solid tumor overexpressing the CD13 receptors. Full article

The foremost concern in forensic entomology is the explicit identification of the species recovered from the crime scene. From the different orders of insects, Diptera is the prime focus in this field, followed by Coleoptera, whose identification can be extremely helpful for corpses in later decomposition stages. In this study, cytochrome oxidase subunit II (COII) was used to check its adequacy as a genetic marker and to create a reference database for eleven species belonging to five families of Coleoptera, namely, Silphidae, Staphylinidae, Histeridae, Dermestidae and Scarabaeidae, from two different states in India to assist in the accurate identification of imperative beetle species in medico-legal entomology. To achieve this, standard protocols of DNA isolation, amplification and sequencing were followed. We concluded that the COII gene can be used as a molecular marker for the identification of forensically relevant species, as observed from the similarities between the phylogenetic relationship constructed by COII and morphological data. Full article