Search Results (231)

Microglia-mediated chronic neuroinflammation is a common pathological feature of Parkinson’s disease (PD). Strong evidence suggests that activated microglia can lesion neurons by releasing exosomes. However, the mechanisms of exosome release from activated microglia remain unclear. We recently revealed a key role of complement receptor 3 (CR3) in regulating microglial activation in the process of progressive neurodegeneration. This study aimed to investigate whether CR3 can regulate exosome release from activated microglia, as well as the underlying mechanisms. We found that LPS, an inducer of microglial M1 activation, induced exosome release from activated microglia. Inhibition of exosome synthesis suppressed LPS-induced microglial activation, gene expression of proinflammatory factors, and related neurotoxicity. Silencing or knocking out CR3 attenuated LPS-induced exosome release in microglia. NADPH oxidase (NOX2) was further identified as a downstream signal of CR3, mediating microglial exosome release and related neurotoxicity. CR3 silencing blocked LPS-induced NOX2 activation and superoxide production through inhibition of p47^phox phosphorylation and membrane translocation. Moreover, NOX2 activation elicited by PMA or supplementation of H₂O₂ recovered exosome release from CR3-silenced microglia. Subsequently, we demonstrated that the CR3-NOX2 axis regulates syntenin-1 to control microglial exosome release. Finally, we observed that the expression of CR3 was increased in the brain of LPS-treated mice, and genetic ablation of CR3 significantly reduced LPS-induced NOX2 activation, microglial M1 polarization, and exosome production in mice. Overall, our findings revealed a critical role of the CR3-NOX2 axis in controlling microglial exosome release and related neurotoxicity through syntenin-1, providing a novel target for the development of a therapeutic strategy for neuroinflammation-mediated neurodegeneration. Full article

As the primary biological risk threatening safe dairy production, bovine mastitis control highly relies on environmental disinfection measures. However, the mechanisms by which chemical disinfectants influence host–environment microbial interactions remain unclear. This study systematically investigated the disinfection efficacy and regulatory effects on microbial community composition and diversity of glutaraldehyde-benzalkonium chloride (BAC) and glutaraldehyde-didecyl dimethyl ammonium bromide (DAB) at recommended concentrations (2–5%), using 80 environmental samples from intensive dairy farms in Xinjiang, China. Combining 16S rDNA sequencing with culturomics, the results showed that BAC achieved a disinfection rate of 99.33%, higher than DAB’s 97.87%, and reduced the environment–gut microbiota similarity index by 23.7% via a cationic bacteriostatic film effect. Microbiome analysis revealed that BAC selectively suppressed Fusobacteriota abundance (15.67% reduction) and promoted Bifidobacterium proliferation (7.42% increase), enhancing intestinal mucosal barrier function through butyrate metabolism. In contrast, DAB induced Actinobacteria enrichment in the environment (44.71%), inhibiting pathogen colonization via bioantagonism. BAC’s long-acting bacteriostatic properties significantly reduced disinfection costs and mastitis incidence. This study first elucidated the mechanism by which quaternary ammonium compound (QAC) disinfectants regulate host health through “environment-gut” microbial interactions, providing a critical theoretical basis for developing precision disinfection protocols integrating “cost reduction-efficiency enhancement-risk mitigation.” Full article

This study investigates the mechanisms by which green finance mitigates non-point source pollution. Based on provincial panel data from China spanning 2005 to 2023, this study conducts an empirical analysis that yields several key findings: (1) The development of green finance significantly reduces the intensity of agricultural non-point source pollution. (2) Green finance indirectly contributes to pollution reduction by incentivizing farmers to adopt environmentally sustainable production practices. (3) The pollution control effects of green finance are amplified in regions with advanced digital infrastructure. (4) The impact of green finance on agricultural pollution demonstrates a threshold effect associated with regional innovation capacity—only when innovation capability exceeds a certain threshold does the emission reduction effect of green finance become evident. Theoretically, this study broadens the research dimensions of green finance by integrating farmer behavioral factors and revealing boundary conditions related to technology and innovation. Policy implications include the need to tailor green financial products for agriculture, accelerate the development of rural digital infrastructure, and implement innovation-driven differentiated policies to enhance precision. Full article

Surimi gel quality is crucial for seafood product texture and water retention, yet conventional processing often fails to maximize the potential of underutilized species like Hypomesus olidus. This study investigated the effects of ultrasonic power (100, 200, 400, 800 W) and time (5, 10, 15, and 20 min) on gel properties to establish optimal processing conditions. Results demonstrated that moderate ultrasonic treatment (200 W, 10 min) significantly enhanced gel quality, yielding a dense, uniform network with improved (p < 0.05) functionality: thr water-holding capacity increased by 35.88%, gel strength increased by 143.75%, and textural properties (hardness, cohesiveness, springiness, gumminess, chewiness) improved by 124.02%, 25%, 8.69%, 201.29%, 188.08% while maintaining color stability (1.59% whiteness increase). These improvements were attributed to optimized protein cross-linking and network formation. These findings provide a scientific basis for the ultrasonic processing of Hypomesus solidus surimi, offering practical parameters for industrial applications to enhance product quality efficiently. Future research should explore scaling effects and synergistic processing methods. Full article

Magnesium alloys are lightweight metals but suffer from high corrosion susceptibility due to their chemical reactivity, limiting their large-scale applications. To enhance corrosion resistance, this work combines Li–Al layered double hydroxides (LDHs) with triethylenetetramine (TETA) inhibitors to form an efficient corrosion protection system. Electrochemical tests, SEM, FT-IR, XPS, and 3D depth-of-field microscopy were employed to evaluate TETA-modified Li–Al LDH coatings at varying concentrations. Among them, the Li–Al LDHs without the addition of a TETA corrosion inhibitor decreased significantly at |Z|_{0.01 Hz} after immersion for 4 h. However, the Li–Al LDHs coating of 23.5 mM TETA experienced a sudden drop at |Z|_{0.01 Hz} after holding for about 60 h, and the Li–Al LDHs coating of 70.5 mM TETA also experienced a sudden drop at |Z|_{0.01 Hz} after holding for about 132 h. By contrast, at the optimal concentration (47 mM), after 24 h of immersion, the maximum |Z|_{0.01 Hz} reached 7.56 × 10⁵ Ω∙cm²—three orders of magnitude higher than pure Li–Al LDH coated AZ31 (2.55 × 10² Ω∙cm²). After 300 h of immersion, the low-frequency impedance remained above 10⁵ Ω∙cm², demonstrating superior long-term protection. TETA modification significantly improved the durability of Li–Al LDHs coatings, addressing the short-term protection limitation of standalone Li–Al LDHs. Li–Al LDHs themselves have a layered structure and effectively capture corrosive Cl⁻ ions in the environment through ion exchange capacity, reducing the corrosion of the interface. Furthermore, TETA exhibits strong adsorption on Li–Al LDHs layers, particularly at coating defects, enabling rapid barrier formation. This inorganic–organic hybrid design achieves defect compensation and enhanced protective barriers. Full article

To address the high vibration and noise in fractional-slot concentrated-winding permanent magnet synchronous machines for electric vehicles, this study focuses on a 30-pole, 36-slot fractional-slot concentrated-winding permanent magnet synchronous machine. These issues are mainly caused by the modulation of high-order radial electromagnetic forces into low-order radial electromagnetic forces, known as the teeth modulation effect. The characteristics of radial electromagnetic forces are analyzed using the Maxwell stress tensor method, and the modulation process is examined. A novel unequal-teeth stator structure is proposed to reduce vibration and noise. Finite element simulations are performed to investigate how this structure affects the amplitude of modulated low-order radial electromagnetic forces. The optimal ratio of the unequal-teeth design is identified to effectively suppress the modulation effect. Simulation results indicate that an appropriately chosen unequal-teeth proportion leads to significant improvements in the machine’s vibration and noise performance across various operating conditions, providing a preliminary validation of the feasibility and effectiveness of the proposed unequal-teeth design methodology. Full article

The interior permanent magnet synchronous motors (IPMSMs) are extensively applied in the field of new energy vehicles due to their high-power density and excellent performance control. However, the iron loss has a significant impact on their performance. This study conducts an optimization analysis on the processing technology of silicon steel sheets and motor structure, targeting the reduction of iron loss and the improvement of the motor’s integrated efficiency. Firstly, the influences of two iron core processing technologies on iron loss, namely gluing and welding, are compared. Through experimental tests, it is found that the iron loss density of the gluing process is lower than that of the welding process, and as the magnetic flux density increases, the difference between the two is expanding. Therefore, the iron loss test data from the adhesive process are employed to develop a variable-coefficient iron loss model, enabling precise calculation of the motor’s iron loss. On this basis, aiming at the problem of excessive iron loss of the motor, a novel topological structure of the stator and rotor is proposed. With the optimization goal of reducing the motor iron loss and taking the connection port of the air magnetic isolation slot and the gap of the stator module as the optimization variables, the optimized design of the IPMSM with low iron loss is achieved based on the Taguchi method. After optimization, the stator iron loss decreases by 13.60%, the rotor iron loss decreases by 20.14%, and the total iron loss is reduced by 15.34%. The optimization scheme takes into account both the electromagnetic performance and the process feasibility, it offers technical backing for the high-efficiency operation of new energy vehicle drive motors. Full article

Talaromycosis (TM) is an invasive fungal infection caused by Talaromyces marneffei (T. marneffei). It has high morbidity and mortality rates, particularly among immunocompromised people. Globally, approximately 17,300 cases and 4900 deaths are reported annually. TM often has vague clinical signs with limited current tests, leading to misdiagnosis, incorrect treatments, or the long-term use of expensive antifungal drugs, which raises healthcare costs and patient risks. Although accurate diagnosis is key for starting the right antifungal therapy and improving outcomes, there are not enough reliable and fast tests. Recent progress with monoclonal antibodies (mAbs) that have high specificity for antigens may boost diagnostic accuracy and cut misdiagnosis rates. This review explores current ways to diagnose TM, including culture, histopathology, and molecular methods such as polymerase chain reaction (PCR) and antigen detection. We also discuss the merits and weaknesses of each method and highlight how mAbs may help diagnose TM. We searched PubMed, Web of Science, and Google Scholar for English-language papers (1990—1 January 2025) using “Talaromycosis” OR “Talaromyces marneffei” plus diagnostic terms (‘diagnosis’, ‘molecular diagnostics’, ‘monoclonal antibody’, ‘lateral flow’, ‘antigen detection’, and ‘fungal diagnosis’). After deduplication and relevance screening, studies with original data or substantive discussion on T. marneffei diagnostics or mAb development were retained to inform this narrative review. Full article

This study systematically investigates the influence of different heat treatment processes on the microstructural evolution and mechanical properties of Cu-15Ni-3Al alloys, with particular emphasis on the synergistic strengthening mechanisms of spinodal decomposition and precipitation hardening. Two distinct aging routes—solution aging and direct aging—were designed to facilitate a comparative assessment of microstructural characteristics and their correlation with mechanical performance. Comprehensive characterization was conducted using scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), and room-temperature tensile testing to elucidate the structure–property relationships. The results reveal that direct aging promotes the formation of fine, coherent L1₂-type Ni₃Al precipitates and the evolution of Ni-enriched regions initially generated through spinodal decomposition into stable Ni₃Al precipitates. These microstructural features act as effective barriers to dislocation motion, thereby significantly enhancing both strength and ductility. The findings provide valuable insights into optimizing heat treatment strategies to improve the performance of Cu-Ni-Al alloys. Full article

In this investigation, the anti-inflammatory potential of phenylspirodrimanes (PSDs) produced by the marine-derived fungal strain Stachybotrys sp. SZU-W23 was systematically explored. A total of 15 PSDs were successfully isolated. Among them, four novel compounds, designated as chartarlactams U-X, were precisely characterized using NMR, HRESIMS, and ECD analyses. Specifically, compound 10 exhibited the most potent inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 macrophages within the 0.3–30 μM concentration range, with an IC₅₀ value of 12.4 μM. Additionally, MTT assays revealed no detectable cytotoxicity at these concentrations. Mechanistic studies revealed that compound 10 effectively suppressed ROS generation, likely inactivating the NF-κB signaling pathway and consequently downregulating pro-inflammatory mediators, including iNOS, IL-6, and IL-1β. Full article

This article profiles 27 innovative advancements in small-molecule drugs approved by the U.S. Food and Drug Administration (FDA) in 2024. These drugs target various therapeutic areas including non-small cell lung cancer, advanced or metastatic breast cancer, glioma, relapsed or refractory acute leukemia, urinary tract infection, Staphylococcus aureus bloodstream infections, nonalcoholic steatohepatitis, primary biliary cholangitis, Duchenne muscular dystrophy, hypertension, anemia due to chronic kidney disease, extravascular hemolysis, primary axillary hyperhidrosis, chronic obstructive pulmonary disease, severe alopecia areata, WHIM syndrome, Niemann–Pick disease type C, schizophrenia, supraventricular tachycardia, congenital adrenal hyperplasia, and cystic fibrosis. Among these approved small-molecule drugs, those with unique mechanisms of action and designated as breakthrough therapies by the FDA represent a significant proportion, highlighting ongoing innovation. Notably, eight of these drugs (including Rezdiffra^®, Voydeya^®, Iqirvo^®, Voranigo^®, Livdelzi^®, Miplyffa^®, Revuforj^®, and Crenessity^®) are classified as “first-in-class” and have received breakthrough therapy designation. These agents not only exhibit distinct mechanisms of action but also offer substantial improvements in efficacy for patients compared to prior therapeutic options. This article offers a comprehensive analysis of the mechanisms of action, clinical trials, drug design, and synthetic methodologies related to representative drugs, aiming to provide crucial insights for future pharmaceutical development. Full article

The seawater refractive index is an important parameter in marine environments, with its variations depending on the specific environmental conditions. During practical applications, modulation parameters such as the sampling rate, bandwidth, and filters directly affect the signal-to-noise ratio (SNR) and need to be adjusted in real-time according to the characteristics of the target signal. Low-cost software-defined radio (SDR) offers significant advantages in this regard. This paper proposes an optical coherence measurement method for seawater refractive index changes based on orthogonal demodulation using SDR along with simulation calculations, and the results demonstrate that the resolution of the refractive index change rate is $3.165\times {10}^{-9}$ RIU/s, corresponding to a refractive index change resolution of ${10}^{-10}$ RIU (frequency range 1 Hz–100 Hz, measurement range 0.1 m). By adopting SDR as the implementation platform for the demodulation algorithm and using a radio-frequency source to simulate interference signals for demodulating the refractive index variation, the results show that the relative error of the SDR demodulation results is below 0.3%. Additionally, this study developed a software-defined optical coherence measurement system for the seawater refractive index and measured the refractive index changes in deionized water during heating. The experimental results showed that the root mean square error (RMSE) of the refractive index changes obtained through SDR demodulation was $5.68\times {10}^{-6}$ RIU. This research provides a novel demodulation method for high-precision measurements of seawater refractive index changes under different marine environments. Full article

Porcine epidemic diarrhea virus (PEDV), a highly infectious alphacoronavirus, has resulted in substantial economic losses within the global swine industry. Existing vaccines and therapeutic agents have proven inadequate in effectively preventing and controlling PEDV. Natural compounds offer distinct advantages in antiviral research due to their abundant availability, diverse biological activities, and low toxicity. In this study, the antiviral properties of the naturally occurring alkaloid 1-deoxynojirimycin (DNJ) against PEDV were examined. The CC₅₀ of DNJ was determined to be 912.5 μM through experimental analysis on Vero-E6 cells. DNJ demonstrated an inhibitory effect on PEDV activity, with a 50% inhibitory concentration (IC₅₀) of 57.76 μM. The compound primarily inhibited PEDV proliferation during the viral life cycle stages of attachment and replication. Moreover, DNJ mitigated the production of reactive oxygen species (ROS) and inflammation associated with PEDV infection. Computational docking predictions suggest that the viral non-structural proteins include Nsp12, Nsp14, and Nsp16 may serve as potential targets for DNJ. Consequently, DNJ represents a promising candidate for the development of novel therapeutic agents against PEDV. Full article

The Yushu yak is one of China’s distinctive yak breeds, primarily distributed in the Yushu Tibetan Autonomous Prefecture of Qinghai Province and its surrounding areas. Yushu yaks are not only economically and culturally significant but also play a crucial role in protecting the ecosystem of the Qinghai-Tibet Plateau and promoting sustainable development. However, there are no clear records regarding the ancestry, population structure, and unique traits of Yushu yaks. Therefore, this study conducted an analysis of genetic diversity, population structure, and selection signals in Yushu yak populations, aiming to provide references for the conservation and utilization of the breed genetic resources. The results of the analysis showed that the Yushu yak population has high genetic diversity and low inbreeding coefficients, indicating a stable genetic structure. Population structure analysis revealed that the Yushu yak lineage is unique, with limited gene flow between domestic and wild yaks. Functional enrichment analysis of positively selected genes in Yushu yaks indicated prominent selection features related to growth and development as well as energy metabolism. Additionally, we classified the Yushu yak breeding bulls into family lineages based on kinship, which is essential for improving the efficiency of utilizing genetic resources and scientifically managing the population. Full article

Sthenoteuthis oualaniensis is one of the most commercially important marine cephalopod species distributed throughout tropical and subtropical waters of the Indo-Pacific Seas. The Indian Ocean is a main fishing ground for S. oualaniensis with a high population density. To explore the distribution of S. oualaniensis in the east equatorial Indian Ocean, four surveys were carried out using light-lift-net fishing vessels. Meanwhile, marine environmental data were also collected, including the sea surface temperature, sea temperature at 100 m depth, mixed layer depth, sea surface chlorophyll-a concentration, sea surface height, and eddy kinetic energy. Generalized Additive Models were used to analyze the relationship between the catch per unit effort (CPUE) for S. oualaniensis and environmental factors. The results showed that the average CPUE of S. oualaniensis was 14.55 kg/h in the four surveys, which was considerably lower than in the South China Sea and Northwest Indian Ocean. In terms of seasonal distribution, the high-CPUE stations were closer to the continental shelf in spring, while they shifted towards the deeper and offshore water in autumn, demonstrating a seasonal migration trend. Pearson correlation analysis showed that CPUE reflected a significant negative correlation with both sea temperature at 100 m depth and eddy kinetic energy (p < 0.001). The Generalized Additive Models revealed that sea surface height was the most significant factor affecting CPUE with a variance explanation of 30.1%. Furthermore, the optimal CPUE prediction model was established by stepwise regression, which contains two factors, sea surface height and eddy kinetic energy, with a variance explanation of 34.9%. This study provides insights into the environmental factors influencing the distribution of S. oualaniensis, which is essential for the sustainable utilization and management of this species. Full article