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Authors = Kezhen Li

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19 pages, 1551 KiB  
Article
Genome-Wide Association Study Reveals Key Genetic Loci Controlling Oil Content in Soybean Seeds
by Xueyang Wang, Min Zhang, Fuxin Li, Xiulin Liu, Chunlei Zhang, Fengyi Zhang, Kezhen Zhao, Rongqiang Yuan, Sobhi F. Lamlom, Honglei Ren, Hongmei Qiu and Bixian Zhang
Agronomy 2025, 15(8), 1889; https://doi.org/10.3390/agronomy15081889 - 5 Aug 2025
Abstract
Seed oil represents a key trait in soybeans, which holds substantial economic significance, contributing to roughly 60% of global oilseed production. This research employed genome-wide association mapping to identify genetic loci associated with oil content in soybean seeds. A panel comprising 341 soybean [...] Read more.
Seed oil represents a key trait in soybeans, which holds substantial economic significance, contributing to roughly 60% of global oilseed production. This research employed genome-wide association mapping to identify genetic loci associated with oil content in soybean seeds. A panel comprising 341 soybean accessions, primarily sourced from Northeast China, was assessed for seed oil content at Heilongjiang Province in three replications over two growing seasons (2021 and 2023) and underwent genotyping via whole-genome resequencing, resulting in 1,048,576 high-quality SNP markers. Phenotypic analysis indicated notable variation in oil content, ranging from 11.00% to 21.77%, with an average increase of 1.73% to 2.28% across all growing regions between 2021 and 2023. A genome-wide association study (GWAS) analysis revealed 119 significant single-nucleotide polymorphism (SNP) loci associated with oil content, with a prominent cluster of 77 SNPs located on chromosome 8. Candidate gene analysis identified four key genes potentially implicated in oil content regulation, selected based on proximity to significant SNPs (≤10 kb) and functional annotation related to lipid metabolism and signal transduction. Notably, Glyma.08G123500, encoding a receptor-like kinase involved in signal transduction, contained multiple significant SNPs with PROVEAN scores ranging from deleterious (−1.633) to neutral (0.933), indicating complex functional impacts on protein function. Additional candidate genes include Glyma.08G110000 (hydroxycinnamoyl-CoA transferase), Glyma.08G117400 (PPR repeat protein), and Glyma.08G117600 (WD40 repeat protein), each showing distinct expression patterns and functional roles. Some SNP clusters were associated with increased oil content, while others correlated with decreased oil content, indicating complex genetic regulation of this trait. The findings provide molecular markers with potential for marker-assisted selection (MAS) in breeding programs aimed at increasing soybean oil content and enhancing our understanding of the genetic architecture governing this critical agricultural trait. Full article
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12 pages, 501 KiB  
Article
An Overview of GEO Satellite Communication Simulation Systems
by Shaoyang Li, Yanli Qi and Kezhen Song
Electronics 2025, 14(13), 2715; https://doi.org/10.3390/electronics14132715 - 4 Jul 2025
Viewed by 354
Abstract
Geostationary Earth orbit (GEO) satellite communication systems have become increasingly significant in global communication networks and national strategic infrastructure, owing to their advantages of extensive coverage, high capacity, and robust reliability. Constructing accurate and reliable simulation systems is essential to support the design, [...] Read more.
Geostationary Earth orbit (GEO) satellite communication systems have become increasingly significant in global communication networks and national strategic infrastructure, owing to their advantages of extensive coverage, high capacity, and robust reliability. Constructing accurate and reliable simulation systems is essential to support the design, evaluation, and optimization of GEO satellite communication systems. This article first reviews the current developments and application prospects of GEO satellite communication systems and highlights the critical role of simulation technologies in system design and performance assessment. Subsequently, a systematic analysis is conducted on two core modules of simulation systems, i.e., coverage analysis and resource management and scheduling. Moreover, this article provides a comprehensive comparison and evaluation of mainstream satellite communication simulation platforms and tools. This review aims to offer valuable insights and guidance for future research and applications in GEO satellite communication simulation, thereby promoting technological innovation and advancement in related fields. Full article
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15 pages, 4081 KiB  
Article
3-Hydroxyacyl CoA Dehydratase 2 Is Essential for Embryonic Development and Hepatic Metabolic Function Under a Low-Fat, High-Carbohydrate Diet
by Lengyun Wei, Fengli Wang, Luoxue Hua, Qun Wang, Benfei Hu, Ziye Yang, Letao Li, Chenfeng Liu and Kezhen Wang
Biology 2025, 14(6), 712; https://doi.org/10.3390/biology14060712 - 17 Jun 2025
Viewed by 495
Abstract
The conversion of carbohydrates into fatty acids is central for energy storage and the development and functioning of organs. Our previous study revealed that Hacd2 deficiency alleviates the fatty liver and diabetes induced by HFD. This study aimed to explore the roles of [...] Read more.
The conversion of carbohydrates into fatty acids is central for energy storage and the development and functioning of organs. Our previous study revealed that Hacd2 deficiency alleviates the fatty liver and diabetes induced by HFD. This study aimed to explore the roles of Hacd2 in organ development and metabolic homeostasis under an LFHCD, which still need to be more deeply explored. We found that the germline deletion of Hacd2 impairs long-chain fatty acid synthesis, which caused embryonic abnormalities after 7.5 days and led to embryonic lethality, as confirmed via photograph and hematoxylin-eosin staining. We next constructed Hacd2LKO mice and found that Hacd2LKO mice were largely normal when fed a chow diet, except for reduced inguinal white adipose tissue formation and glucose metabolism. Meanwhile, under an LFHCD, Hacd2 deletion markedly controlled body weight and white adipose tissue formation, leading to lower cholesterol and triglycerides in serum; however, it unexpectedly resulted in enlarged liver volume, hepatocyte swelling and nuclear abnormalities, and infiltration of inflammatory cells, including macrophages, neutrophils and dendritic cells. Furthermore, inhibition of Hacd2 also reduced triglyceride levels and the expression of related lipogenic genes during adipocyte differentiation, as confirmed via RNA interference analysis. These findings highlight the critical roles of Hacd2 in embryonic development and metabolic diseases, revealing its protective function in maintaining liver homeostasis under an LFHCD. Therefore, targeted interventions involving Hacd2 for metabolic diseases must take into account dietary changes and the functioning of the liver. Full article
(This article belongs to the Special Issue Animal Models of Metabolic Diseases)
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21 pages, 4462 KiB  
Article
Study on the Self-Healing Performance of Polyurethane/Graphene Oxide-Modified Asphalt Based on Dynamic Disulfide Bonds
by Guokai Li, Min Wang, Kezhen Yan and Xiaojin Song
Materials 2025, 18(11), 2549; https://doi.org/10.3390/ma18112549 - 29 May 2025
Viewed by 542
Abstract
In this study, an investigation on using polyurethane/graphene oxide (PU/GO) containing disulfide bonds as a modifier to improve the self-healing ability of asphalt was conducted. PU/GO with different GO contents were synthesized and modified asphalt with different PU/GO dosages (2%, 4%, 6%, 8%) [...] Read more.
In this study, an investigation on using polyurethane/graphene oxide (PU/GO) containing disulfide bonds as a modifier to improve the self-healing ability of asphalt was conducted. PU/GO with different GO contents were synthesized and modified asphalt with different PU/GO dosages (2%, 4%, 6%, 8%) were also prepared. The effect of GO contents on the mechanical and self-healing properties of PU/GO was explored and the impacts of PU/GO contents on the basic properties and self-healing properties of asphalt were also investigated. The results indicated that GO could significantly improve the mechanical properties of PU, as the tensile strength of PU/GO with 1.6% GO increased by more than 100% compared with pure PU. Moreover, GO also had a positive impact on the early-stage self-healing properties of PU/GO. PU/GO could be well dispersed in asphalt and clearly improve the low-temperature performance of base asphalt. When the PU/GO content is 8%, the ductility of modified asphalt was almost 6 times that of base asphalt. The results of both ductility and BBR self-healing tests revealed that the addition of PU/GO improved the self-healing properties of asphalt under room temperature and infrared light conditions. Especially under infrared light conditions, the ductility self-healing coefficient of 8% PU/GO-modified asphalt could reach 100% after healing for 15 min. Full article
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28 pages, 24594 KiB  
Article
Cyclic Behavior of Joints Assembled Using Prefabricated Beams and Columns with High-Ductility Recycled Powder Concrete
by Xiuling Li, Haodong Sun, Kezhen Chen, Tianfeng Yuan, Long Wen, Xiaowei Zheng and Tongxing Bu
Buildings 2025, 15(5), 838; https://doi.org/10.3390/buildings15050838 - 6 Mar 2025
Viewed by 794
Abstract
The integration of recycled powder (RP) as a partial cement replacement in concrete, combined with fiber reinforcement, facilitates the development of high-ductility recycled powder concrete (HDRPC) with enhanced mechanical properties. This approach holds significant potential for effectively recycling construction waste and reducing carbon [...] Read more.
The integration of recycled powder (RP) as a partial cement replacement in concrete, combined with fiber reinforcement, facilitates the development of high-ductility recycled powder concrete (HDRPC) with enhanced mechanical properties. This approach holds significant potential for effectively recycling construction waste and reducing carbon emissions. To improve the seismic performance of prefabricated joints in industrial prefabricated building production, experimental tests under low-cycle reversed cyclic loading were conducted on four HDRPC prefabricated joints, one HDRPC cast-in-place joint, and one normal prefabricated concrete joint. The study systematically analyzed damage patterns, deformation ductility, stiffness degradation, hysteresis energy dissipation, and other performance characteristics. The results demonstrate that HDRPC effectively mitigates crack width and shear deformation in the joint core area, achieving a 17.8% increase in joint-bearing capacity and a 33.3% improvement in displacement ductility. Moreover, HDRPC improves specimen damage characteristics, enhances joint shear capacity and flexibility, and reduces the demand for hoop reinforcement in the joint core area due to its exceptional shear ductility. Based on the softened tension–compression bar model, a crack-resistance-bearing capacity equation for HDRPC joints was derived, which aligns closely with shear test results when cracks develop in the joint core area. Full article
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23 pages, 5532 KiB  
Article
Characterization of Leptin and Leptin Receptor Gene in the Siberian Sturgeon (Acipenser baerii): Molecular Cloning, Tissue Distribution, and Its Involvement in Feeding Regulation
by Hongwei Wu, Jiamei Li, Kezhen Jiang, Yingzi Li, Zhaoxiong Yu, Bin Wang, Bo Zhou, Xin Zhang, Ni Tang and Zhiqiong Li
Int. J. Mol. Sci. 2025, 26(5), 1968; https://doi.org/10.3390/ijms26051968 - 25 Feb 2025
Viewed by 738
Abstract
Leptin is an adipokine known as a regulator of feeding and metabolism in mammals. Previous studies on fish have revealed its role in food intake regulation in limited teleosts. However, its specific function in Siberian sturgeon, an ancient Chondrostei fish, remains poorly understood. [...] Read more.
Leptin is an adipokine known as a regulator of feeding and metabolism in mammals. Previous studies on fish have revealed its role in food intake regulation in limited teleosts. However, its specific function in Siberian sturgeon, an ancient Chondrostei fish, remains poorly understood. This study represents the first successful cloning of sequences for leptin and leptin receptors in Siberian sturgeon, achieved using RT-PCR. The predicted leptin sequence in this species consists of 168 amino acids that exhibit low identity with other fish species, except within the Acipenseriformes order. Tissue distribution analysis revealed a high expression of Siberian sturgeon leptin mRNA in the liver and lepr mRNA in the hypothalamus. Fasting differentially affected the expression of leptin and lepr mRNA, with decreased levels in the hypothalamus and increased levels in the liver (leptin: 3–15 days; lepr: 6–15 days). Recombinant Siberian sturgeon leptin (Ssleptin) was produced via E. coli expression, and intraperitoneal injection (100 ng/g BW) significantly inhibited food intake. The anorectic effect was correlated with changes in hypothalamic gene expression, including downregulation of orexigenic factors (agrp, orexin, npy, and ghrelin) and upregulation of anorexigenic factors (pomc, mch, and insulin). Meanwhile, the peripheral administration of Ssleptin promoted the expression of resistin in the liver and concurrently increased cck and pyy mRNA levels in the valvular intestine. Furthermore, Ssleptin injection stimulated the expression of hypothalamic lepr, jak2, akt, and ampkα2 mRNA. These findings suggest that leptin plays a significant role in the feeding control of Siberian sturgeon and provide new insights into the evolutionary function of leptin in fish. Full article
(This article belongs to the Special Issue Fish Nutrition, Metabolism and Physiology)
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24 pages, 9528 KiB  
Article
Study on Optimization of Rice-Drying Process Parameters and Directional Regulation of Nutrient Quality
by Jinquan Li, Kezhen Chang, Jun Yin, Yi Jin, Xiaokang Yi, Zhongjie Zhang, Yichuan He, Qiaonan Yang, Zhihui Tang, Xiaoyu Liu and Wenfu Wu
Appl. Sci. 2024, 14(23), 10825; https://doi.org/10.3390/app142310825 - 22 Nov 2024
Viewed by 1454
Abstract
The physicochemical components of rice such as starch, protein, fat and water have significant influence on its nutritional value, and the drying process can easily cause changes in these components. In this paper, the effect of technical parameters on the nutritional quality of [...] Read more.
The physicochemical components of rice such as starch, protein, fat and water have significant influence on its nutritional value, and the drying process can easily cause changes in these components. In this paper, the effect of technical parameters on the nutritional quality of rice during hot-air drying was studied, and a control method of the rice-drying process based on effective accumulated temperature was proposed to ensure the drying quality and improve the drying efficiency. Through thin-layer drying experiments, hot-air temperature (T), humidity (RH), initial moisture content (MC), wind speed (V) and tempering ratio (TR) were selected as control factors, and the central composite design was adopted to optimize the experimental scheme. The relationship between each factor and nutrient quality was revealed through response surface analysis, and the regression model and process optimization parameters were established. The results show that the optimization parameters are as follows: hot-air temperature, 48.87 °C; humidity, 30.12%; initial moisture content, 21.31%; wind speed, 0.62 m/s; tempering ratio, 2.87; the optimized total drying time is 4.23 h; the effective accumulated temperature is 214.44 °C·h. The contents of protein, fat, amylose and amylopectin were 8.47 g/100 g, 1.97 g/100 g, 15.33 g/100 g and 60.50 g/100 g, respectively. The relative error of the verification test was 4.17%. The optimized process can effectively maintain the nutritional quality of rice and improve drying efficiency. This paper provides a new way to deeply explore the mechanism of rice quality change, and the established process reference chart provides a scientific basis for actual drying operations and the development of an intelligent control system. Full article
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20 pages, 508 KiB  
Review
Development of Grain Dryer Control Technology from the Perspective of Low Carbon and Intelligentization
by Kezhen Chang, Jinquan Li, Yi Jin and Chunshan Liu
Appl. Sci. 2024, 14(22), 10587; https://doi.org/10.3390/app142210587 - 17 Nov 2024
Cited by 1 | Viewed by 2872
Abstract
The grain-drying process plays a critical role in grain storage and quality assurance. In recent years, with the advancement of low-carbon and intelligent technologies, the control technology of grain dryers has significantly improved. This paper systematically reviews the development status of grain dryer [...] Read more.
The grain-drying process plays a critical role in grain storage and quality assurance. In recent years, with the advancement of low-carbon and intelligent technologies, the control technology of grain dryers has significantly improved. This paper systematically reviews the development status of grain dryer control technology from the perspective of low-carbon and intelligentization, analyzing the technological differences in control systems between domestic and international approaches. Current research challenges include the insufficient integration of control technologies with the drying process, limited control variables, the inadequate application of intelligent control strategies, and unstable sensor accuracy. To enhance the performance of grain-drying systems, this paper suggests optimizing control mechanisms, adopting efficient and environmentally friendly energy sources, improving sensor performance, introducing advanced intelligent control algorithms, and strengthening system monitoring capabilities. Looking ahead, with the further integration of AI, IoT, and green energy, grain-drying control systems are expected to evolve towards greater intelligence, remote operation, and low carbonization, providing technical support for enhancing drying efficiency and environmental performance. Full article
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13 pages, 836 KiB  
Article
Associations between Ethylene Oxide Exposure and Liver Function in the US Adult Population
by Shanshan Li, Jinzhou Wang, Dengliang Lei, Dadi Peng, Kezhen Zong, Kaili Li, Zhongjun Wu, Yanyao Liu and Zuotian Huang
Toxics 2024, 12(8), 551; https://doi.org/10.3390/toxics12080551 - 30 Jul 2024
Cited by 4 | Viewed by 1763
Abstract
Background: Ethylene oxide, a reactive epoxy compound, has been widely used in various industries for many years. However, evidence of the combined toxic effects of ethylene oxide exposure on the liver is still lacking. Methods: We analyzed the merged data from the National [...] Read more.
Background: Ethylene oxide, a reactive epoxy compound, has been widely used in various industries for many years. However, evidence of the combined toxic effects of ethylene oxide exposure on the liver is still lacking. Methods: We analyzed the merged data from the National Health and Nutrition Examination Survey (NHANES) from 2013 to 2016. Ultimately, 4141 adults aged 18 and over were selected as the sample. We used linear regression to explore the association between blood ethylene oxide and LFT indicators. Results: The weighted linear regression model showed that HbEO is positively correlated with ALP (β = 2.61, 95% CI 1.97, 3.24, p < 0.0001), GGT (β = 5.75, 95% CI 4.46, 7/05, p < 0.0001), ALT (β = 0.50, 95% CI 0.09, 0.90, p = 0.0158), and AST (β = 0.71, 95% CI 0.44, 0.98, p < 0.0001) and negatively correlated with TBIL (β = −0.30, 95% CI −0.43, −0.16, p < 0.0001). Conclusions: Ethylene oxide exposure is significantly associated with changes in liver function indicators among adults in the United States. Future work should further examine these relationships. Full article
(This article belongs to the Special Issue Environmental Contaminants and Human Health)
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18 pages, 4440 KiB  
Review
Recent Progress in Photocatalytic Degradation of Water Pollution by Bismuth Tungstate
by Yingjie Zhang, Huijuan Yu, Ruiqi Zhai, Jing Zhang, Cuiping Gao, Kezhen Qi, Li Yang and Qiang Ma
Molecules 2023, 28(24), 8011; https://doi.org/10.3390/molecules28248011 - 8 Dec 2023
Cited by 21 | Viewed by 4497
Abstract
Photocatalysis has emerged as a highly promising, green, and efficient technology for degrading pollutants in wastewater. Among the various photocatalysts, Bismuth tungstate (Bi2WO6) has gained significant attention in the research community due to its potential in environmental remediation and [...] Read more.
Photocatalysis has emerged as a highly promising, green, and efficient technology for degrading pollutants in wastewater. Among the various photocatalysts, Bismuth tungstate (Bi2WO6) has gained significant attention in the research community due to its potential in environmental remediation and photocatalytic energy conversion. However, the limited light absorption ability and rapid recombination of photogenerated carriers hinder the further improvement of Bi2WO6’s photocatalytic performance. This review aims to present recent advancements in the development of Bi2WO6-based photocatalysts. It delves into the photocatalytic mechanism of Bi2WO6 and summarizes the achieved photocatalytic characteristics by controlling its morphology, employing metal and non-metal doping, constructing semiconductor heterojunctions, and implementing defective engineering. Additionally, this review explores the practical applications of these modified Bi2WO6 photocatalysts in wastewater purification. Furthermore, this review addresses existing challenges and suggests prospects for the development of efficient Bi2WO6 photocatalysts. It is hoped that this comprehensive review will serve as a valuable reference and guide for researchers seeking to advance the field of Bi2WO6 photocatalysis. Full article
(This article belongs to the Special Issue Recent Advances in Transition Metal Catalysis)
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15 pages, 7243 KiB  
Article
Arbuscular Mycorrhizal Fungi and Diazotrophic Diversity and Community Composition Responses to Soybean Genotypes from Different Maturity Groups
by Mandi Wu, Shengzhican Li, Jie Bai, Kezhen Wang, Yang Qu, Mingxiu Long, Peizhi Yang, Tianming Hu and Shubin He
Agronomy 2023, 13(7), 1713; https://doi.org/10.3390/agronomy13071713 - 26 Jun 2023
Cited by 1 | Viewed by 1636
Abstract
Soybeans can simultaneously form tripartite symbiotic associations with arbuscular mycorrhizal fungi (AMF) and diazotrophs. However, no studies have explored whether soybean genotypes differing in their maturity groups (MGs) may have implications for the recruitment of rhizosphere soil AMF and diazotrophs. We investigated the [...] Read more.
Soybeans can simultaneously form tripartite symbiotic associations with arbuscular mycorrhizal fungi (AMF) and diazotrophs. However, no studies have explored whether soybean genotypes differing in their maturity groups (MGs) may have implications for the recruitment of rhizosphere soil AMF and diazotrophs. We investigated the diversity and community compositions of AMF and diazotrophs in three soybean genotypes differing in their maturity groups (MG) using high-throughput sequencing. The soybean MGs were MG1.4, MG2.2, and MG3.8, representing early, standard, and late maturity, respectively, for the study region. Soil chemical properties and yield-related traits were determined, and co-occurrence network patterns and drivers were also analyzed. The results obtained demonstrated that AMF richness and diversity were relatively stable in the three soybean genotypes, but noticeable differences were observed in diazotrophs, with late maturity being significantly higher than early maturity. However, there were differences in AMF and diazotrophic composition among different MG genotypes, and the changes in the proportion of dominant species in the community were necessarily related to MG genotypes. Co-occurrence network analysis showed that the positive correlation between AMF and diazotrophs gradually decreased in earlier MG genotypes than in the other later MG genotypes. The results of the structural equation model analysis showed that soil organic carbon, AMF, diversity of soil nutrients, and extracellular enzyme activities were important factors driving soybean yield change, with organic carbon accounting for more than 80% of the pathways analyzed. These results suggest that soybean genotype selection based on MG plays an important role in recruiting both AMF and diazotrophic communities, and in comparison to AMF, diazotrophs are more responsive to the different MG genotypes. Full article
(This article belongs to the Special Issue Advances in Stress Biology of Forage and Turfgrass)
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13 pages, 3363 KiB  
Article
Identification of Candidate Genes for Drought Resistance during Soybean Seed Development
by Xiaofei Tang, Yongguo Xue, Dan Cao, Xiaoyan Luan, Kezhen Zhao, Qi Liu, Yang Ren, Zifei Zhu, Yong Li and Xinlei Liu
Agriculture 2023, 13(5), 949; https://doi.org/10.3390/agriculture13050949 - 25 Apr 2023
Cited by 5 | Viewed by 2539
Abstract
Drought is the main abiotic stress limiting soybean production worldwide and seriously limits the reproductive growth of soybean. To elucidate the molecular mechanisms of drought stress response in soybean seed developmental stages, transcriptome analysis was performed on five seed developmental stages under drought [...] Read more.
Drought is the main abiotic stress limiting soybean production worldwide and seriously limits the reproductive growth of soybean. To elucidate the molecular mechanisms of drought stress response in soybean seed developmental stages, transcriptome analysis was performed on five seed developmental stages under drought stress and control conditions. Thousands of genes were found to change in expression level under drought stress, GO and KEGG enrichment analysis showed that differentially expressed genes (DEGs) were involved in drought resistance at every stage or tissue, but there were very few DEGs at the primary stage of seed development, which indicated that the seeds at the primary developmental stage were influenced little by drought stress. There was no shared DEG among all five stages or tissues, this indicated that they have different mechanisms to resist drought stress. Furthermore, we investigated DEGs associated with drought response at different stages to identify candidate regulators of drought stress response during soybean seed development. These genes are associated with metabolic and regulatory networks, among others. Taken together, the results will expand our understanding of the adaptation of soybean to drought stress and provide new insights into the molecular mechanisms of soybean drought tolerance development. Full article
(This article belongs to the Section Crop Genetics, Genomics and Breeding)
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26 pages, 1533 KiB  
Review
Application of Single-Cell RNA Sequencing in Ovarian Development
by Xiaoqin Gong, Yan Zhang, Jihui Ai and Kezhen Li
Biomolecules 2023, 13(1), 47; https://doi.org/10.3390/biom13010047 - 27 Dec 2022
Cited by 24 | Viewed by 8861
Abstract
The ovary is a female reproductive organ that plays a key role in fertility and the maintenance of endocrine homeostasis, which is of great importance to women’s health. It is characterized by a high heterogeneity, with different cellular subpopulations primarily containing oocytes, granulosa [...] Read more.
The ovary is a female reproductive organ that plays a key role in fertility and the maintenance of endocrine homeostasis, which is of great importance to women’s health. It is characterized by a high heterogeneity, with different cellular subpopulations primarily containing oocytes, granulosa cells, stromal cells, endothelial cells, vascular smooth muscle cells, and diverse immune cell types. Each has unique and important functions. From the fetal period to old age, the ovary experiences continuous structural and functional changes, with the gene expression of each cell type undergoing dramatic changes. In addition, ovarian development strongly relies on the communication between germ and somatic cells. Compared to traditional bulk RNA sequencing techniques, the single-cell RNA sequencing (scRNA-seq) approach has substantial advantages in analyzing individual cells within an ever-changing and complicated tissue, classifying them into cell types, characterizing single cells, delineating the cellular developmental trajectory, and studying cell-to-cell interactions. In this review, we present single-cell transcriptome mapping of the ovary, summarize the characteristics of the important constituent cells of the ovary and the critical cellular developmental processes, and describe key signaling pathways for cell-to-cell communication in the ovary, as revealed by scRNA-seq. This review will undoubtedly improve our understanding of the characteristics of ovarian cells and development, thus enabling the identification of novel therapeutic targets for ovarian-related diseases. Full article
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17 pages, 3327 KiB  
Article
Catalytic Pyrolysis of Waste Plastics over Industrial Organic Solid-Waste-Derived Activated Carbon: Impacts of Activation Agents
by Kezhen Qian, Wenmin Tian, Wentao Li, Shutong Wu, Dezhen Chen and Yuheng Feng
Processes 2022, 10(12), 2668; https://doi.org/10.3390/pr10122668 - 12 Dec 2022
Cited by 9 | Viewed by 4159
Abstract
Renewable source-derived carbon is found to be a green alternative catalyst to zeolite for the pyrolysis of plastics. However, only polyethylene (PE) catalytic pyrolysis over biomass-derived carbon has been extensively studied. In this work, carbon was produced from industrial organic solid waste using [...] Read more.
Renewable source-derived carbon is found to be a green alternative catalyst to zeolite for the pyrolysis of plastics. However, only polyethylene (PE) catalytic pyrolysis over biomass-derived carbon has been extensively studied. In this work, carbon was produced from industrial organic solid waste using different activation agents, and their catalytic performance on the thermal degradation of typical polymers, namely PE, polypropylene (PP), polystyrene (PS), and polyethylene terephthalate (PET) were investigated. The degradation mechanisms and the roles of different active sites of the carbons are discussed. Steam failed to activate the carbon, which has a low specific surface area (6.7 m2/g). Chemical activation using H3PO4 and ZnCl2 produces carbons with higher specific surface area and more porosity. The pyrolysis characteristics of LDPE, PP, PS, and PET catalyzed by the carbons were studied using TGA and a fixed-bed reactor. The thermogravimetric results indicate that all three carbons reduce the pyrolysis temperature. The analysis of the products shows that the P- and Zn-involved acid sites on the AC-HP and AC-ZN change the reaction pathway of plastics and promote: (1) C-C cracking and aromatization of polyolefins; (2) the protonation of phenyl carbon of PS to yield higher benzene, toluene, and ethylbenzene; and (3) the decarboxylation of the terephthalic acid intermediate of PET, resulting in higher CO2 and benzene. In addition, the high-value chemicals, long-chain alkylbenzenes, were found in the liquids of AC-ZN and AC-HP. The long-chain alkylbenzenes are probably formed by acid-catalyzed alkylation of aromatic hydrocarbons. This study provides basic data for the development of a cheap catalyst for plastic pyrolysis. Full article
(This article belongs to the Special Issue Advanced Technology of Biomass Gasification Processes)
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10 pages, 1934 KiB  
Article
Black Phosphorus/Carbon Nanoframes for Efficient Flexible All-Solid-State Supercapacitor
by Zunbin Duan, Danni Liu, Zhaoer Ye, Caixia Sun, Zikun Wang, Kezhen Chen, Yang Li, Hao Huang, Xiaoliang Zeng, Jiahong Wang, Rong Sun and Xue-Feng Yu
Nanomaterials 2022, 12(19), 3311; https://doi.org/10.3390/nano12193311 - 23 Sep 2022
Cited by 5 | Viewed by 2590
Abstract
A flexible all-solid-state supercapacitor with fast charging speed and high power density is a promising high-performance energy storage and sensor device in photovoltaic systems. Two-dimensional black phosphorus (BP) is a prospective electrode nanomaterial, but it struggles to fully exert its properties limited by [...] Read more.
A flexible all-solid-state supercapacitor with fast charging speed and high power density is a promising high-performance energy storage and sensor device in photovoltaic systems. Two-dimensional black phosphorus (BP) is a prospective electrode nanomaterial, but it struggles to fully exert its properties limited by its self-stacking. Herein, by embedding carbon nanoparticles into the interlayer of BP microplates, the designed BP/carbon nanoframe (BP/C NF) forms a certain nano-gap on the substrate for promoting the orderly transport of charges. The corresponding supercapacitor BP/C SC has a capacity of 372 F g−1, which is higher than that constructed from BP microplates (32.6 F g−1). Moreover, the BP/C SC exhibits good stability with a ca. 90% of capacitance retentions after 10,000 repeated bending and long-term cycles. Thus, the proposed strategy of using BP/carbon nanoframes is feasible to develop exceptional flexible energy devices, and it can guide the design of relevant two-dimensional nanocomposites. Full article
(This article belongs to the Special Issue Solar Thin Film Nanomaterials and Nanodevices)
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