Search Results (171)

Background: Peritoneal stretching from CO₂ insufflation is a primary mechanism of pain associated with laparoscopy. Cyclooxygenase-2 inhibitors are promising anti-inflammatory and analgesic agents. This study aimed to evaluate the effect of celecoxib on postoperative pain reduction and associated changes in peritoneal gene expression after laparoendoscopic single-site (LESS) surgery for benign gynecologic disease. Methods: In this randomized, double-blind, placebo-controlled pilot study, 70 patients were randomly assigned to receive either celecoxib or placebo (400 mg) 40 min before surgery. Peritoneal tissues were collected before and after CO₂ insufflation. We analyzed changes in expressions of prostaglandin I₂ synthase, prostaglandin E synthase (PTGES), PTGES3, aldo-keto reductase family 1 member C1, and 15-hydroxyprostaglandin dehydrogenase (HPGD). Numeric Rating Scale (NRS) pain scores were also compared between groups. Results: A total of 62 patients completed the study: 30 in the celecoxib group and 32 in the placebo group. The mean CO₂ exposure time was 60.4 min. In a quantitative real-time polymerase chain reaction analysis, HPGD mRNA expression significantly increased after surgery in patients exposed to CO₂ for more than 60 min. Patients treated with celecoxib showed a significantly higher rate of grade 3 expression (83.3% vs. 37.5%; p = 0.01) and a level 2 increase in HPGD expression on in situ hybridization (58.3% vs. 12.5%; p = 0.01), despite no significant difference on immunohistochemistry. Moreover, celecoxib effectively reduced NRS pain scores compared to placebo. Conclusions: In this pilot study, celecoxib appeared to reduce postoperative pain and was associated with increased HPGD mRNA expression in the peritoneal tissue of patients with prolonged CO₂ exposure during LESS surgery. These exploratory findings warrant confirmation in larger trials with functional validation of HPGD expression (ClinicalTrials.gov, NCT03391570). Full article

In this study, we investigated the effects of MG4244 on intestinal permeability, oxidative stress, and lipid accumulation in Caenorhabditis elegans with metabolic inflammation induced by Pseudomonas aeruginosa (PA) and a high-glucose diet (HGD). The worms infected with PA exhibited increased intestinal permeability and reactive oxygen species (ROS) production, which were improved upon MG4244 treatment. Also, MG4244 inhibited lipid and ROS accumulation induced by an HGD. In addition, MG4244-treated worms showed extended lifespans under various conditions. To elucidate the mechanism of the MG4244 effects, we conducted further investigation using mutant strains with knockdown of genes associated with the AMP-activated protein kinase (AMPK) and mitogen-activated protein kinase (MAPK) pathways. The results demonstrated that the MG4244 effect on lipid metabolism was primarily mediated through the AMPK signaling pathway. Furthermore, MG4244 enhanced pathogen resistance by MAPK signaling pathways, mitigating stress responses, and maintaining intestinal integrity. In further studies, combined treatment with PA and an HGD significantly increased intestinal permeability, lipid, and ROS levels, confirming their negative synergistic effects. However, MG4244 under PA and HGD co-treatment conditions effectively mitigated these health disruptions, suggesting a protective role of MG4244. This study provides an in vivo platform using C. elegans to evaluate probiotic efficacy related to the intestinal environment. Also, our results highlight the therapeutic potential of MG4244 in improving resilience to metabolic inflammation through gut-targeted mechanisms. Full article

This research assesses four Shared Socioeconomic Pathway (SSP) scenarios (SSP1-2.6, SSP2-4.5, SSP3-7.0, SSP5-8.5) concerning precipitation quantiles across Korea, utilizing the CMIP6 multi-model ensemble comprising 23 General Circulation Models alongside observational data to project future changes. Precipitation quantiles, derived from regional frequency analysis conducted at 615 sites, are calculated as annual averages for the period from 2015 to 2024. Each SSP scenario is evaluated for its spatial distribution through the application of observational data and chi-square tests, with the results indicating that the SSP3-7.0 ensemble most accurately reflects the current quantile estimates derived from observational data. Furthermore, interannual precipitation quantiles are projected to extend to the year 2100 to discern long-term trends within each reproducible period. It is anticipated that precipitation associated with the 100-year reproducible period will increase by over 20% in most regions across the nation by the century’s end, with this increase becoming more pronounced in accordance with the severity of the pathway. These findings underscore significant increases in extreme rainfall events under high-emission scenarios and highlight the critical need for the integration of enhanced flood mitigation, water resource management, and climate adaptation strategies within Korea’s planning framework. Full article

Background/Objectives: We aimed to discover genes with bimodal expression linked to patient outcomes, to reveal underlying oncogenotypes and identify new therapeutic insights in lung adenocarcinoma (LUAD). Methods: We performed meta-analysis to screen LUAD datasets for prognostic genes with bimodal expression patterns. Kynureninase (KYNU), a key enzyme in tryptophan catabolism, emerged as a top candidate. We then examined its relationship with LUAD mutations, metabolic alterations, immune microenvironment states, and expression patterns in human and mouse models using bulk and single-cell transcriptomics, metabolomics, and preclinical model datasets. Pan-cancer prognostic associations were also assessed. Results: Model-based clustering of KYNU expression outperformed median-based dichotomization in prognostic accuracy. KYNU was elevated in tumors with KEAP1 and STK11 co-mutations but remained a strong independent prognostic marker. Metabolomic analysis showed that KYNU-high tumors had increased anthranilic acid, a catalytic product, while maintaining stable kynurenine levels, suggesting a compensatory mechanism sustaining immunosuppressive signaling. Single-cell and bulk data showed KYNU expression was cancer cell-intrinsic in immune-cold tumors and myeloid-derived in immune-infiltrated tumors. In murine LUAD models, Kynu expression was predominantly immune-derived and uncoupled from Nrf2/Lkb1 signaling, indicating poor model fidelity. KYNU’s prognostic associations extended across cancer types, with poor outcomes in pancreatic and kidney cancers but favorable outcomes in melanoma, underscoring the need for lineage-specific considerations in therapy development. Conclusions:KYNU is a robust prognostic biomarker and potential immunometabolic target in LUAD, especially in STK11 and KEAP1 co-mutated tumors. Its cancer cell-intrinsic expression and immunosuppressive metabolic phenotype offer translational potential, though species-specific expression patterns pose challenges for preclinical modeling. Full article

This double-blind, randomized, placebo-controlled clinical study evaluated the efficacy of a shampoo containing Plantago asiatica L. on hair health in adults aged 20–60. Following a 4-week wash-out period, participants used either the test shampoo or a placebo for 12 weeks. Hair measurements using phototrichograms and hair count tests were conducted at baseline and at weeks 4, 8, and 12. The test group demonstrated progressive improvements in hair strand thickness (0.009 mm, 0.017 mm, and 0.020 mm at weeks 4, 8, and 12, respectively) and hair density (0.9 hairs/cm² at weeks 4 and 8, 1.1 hairs/cm² at week 12). Additionally, a significant reduction in hair shedding was observed compared to the placebo group. These findings suggest that shampoo containing Plantago asiatica L. effectively improves hair thickness and density while reducing hair loss, offering a natural solution for hair care concerns. Full article

Ovarian cancer remains one of the most lethal gynecologic malignancies, primarily due to late-stage diagnosis, high recurrence rates, and the development of chemoresistance. Although targeted therapies have improved patient outcomes, their efficacy is often limited by off-target toxicity and acquired drug resistance. Extracellular vesicles (EVs), nanoscale vesicles naturally released by cells, have emerged as promising carriers for precision drug delivery. This review provides a comprehensive overview of recent advances in EV-based therapeutic strategies for ovarian cancer, including the delivery of chemotherapeutic agents, nucleic acid therapeutics, and immunomodulatory molecules. We further explore innovative engineering approaches to enhance targeting specificity, such as surface modification, cell source selection, biomaterial integration, and magnetic nanoparticle-assisted delivery. Key translational challenges in bringing EV-based therapies to clinical application are also addressed. Collectively, these insights underscore the transformative potential of EV-based platforms in advancing targeted and personalized treatment for ovarian cancer. Full article

Background/Objectives: Microbial biofilms on denture surfaces pose significant oral and systemic health risks. Although chemical denture cleansers are widely used, they can cause mucosal irritation and disrupt the oral microbiome. The aim of this study was to evaluate the antimicrobial efficacy and biocompatibility of a denture cleanser containing Paeonia lactiflora extract (DC-PL) as a potential natural alternative. Methods: Oral microcosm biofilms were formed using human saliva and matured over 6 days. Then, the biofilms were treated for 1 min daily over 6 days with DC-PL, distilled water (DW; negative control), or Polident^® (PD; positive control). Antimicrobial effects were assessed by measuring the red fluorescence intensity (ratio of red to green fluorescence intensity [Ratio_R/G]) and aciduric bacterial counts. Biocompatibility was evaluated through an oral mucosal irritation test. A one-way analysis of variance followed by Tukey’s post hoc test was used for between-group comparisons. Results: Ratio_R/G in the DC-PL group was significantly lower than that in the DW group (0.94-fold, p = 0.021) and comparable with that in the PD group (p = 0.502). Aciduric bacterial counts in the DC-PL group were 0.92-fold lower than those in the DW group (p = 0.037) and comparable with those in the PD group (p = 0.460). The oral mucosal irritation index was 0, indicating no irritation. Conclusions: DC-PL demonstrated antimicrobial efficacy similar to that of PD while maintaining excellent biocompatibility. These findings underscore its potential as a safe and effective alternative to conventional chemical cleansers, offering a clinically viable solution for improving oral health management. Full article

Fracture toughness is a key property of epoxy resins with a high glass transition temperature (T_g), used in carbon fiber/epoxy composites for aerospace applications. Conventional toughening methods rely on adding toughening agents, often compromising the processibility and thermal stability. This study introduces a simple self-toughening approach that enhances the fracture toughness without sacrificing other properties by controlling the cured epoxy network structure. Tetraglycidyl 4,4′-diaminodiphenylmethane (TGDDM) epoxy resin was cured using mixtures of structural isomeric curing agents, 3,3′- and 4,4′-diaminodiphenyl sulfone (3,3′- and 4,4′-DDS), at ratios of 7:3, 5:5, and 3:7. The optimal 7:3 ratio produced a resin with 30% higher fracture toughness compared to TGDDM/3,3′-DDS and 100% higher than the TGDDM/4,4′-DDS system. The T_g of the self-toughened resin ranged from 241 to 266 °C, which was intermediate between the T_g values of the TGDDM/3,3′-DDS and TGDDM/4,4′-DDS systems. This improvement is attributed to the higher crosslink density and reduced free volume of the epoxy network. These findings demonstrate that simply mixing isomeric curing agents enables self-toughening, providing a practical and efficient strategy to enhance the performance of high-T_g epoxy resins in advanced composite applications. Full article

The Government of Korea uses green remodeling (GR) as a central policy for achieving carbon neutrality in the building sector. However, despite GR’s energy-saving benefits, it raises embodied carbon (EC) due to the incorporation of new materials, and there is a lack of impact analysis and assessment research. Thus, this study established the GR-LCA methodology to evaluate the environmental impacts (EIs) of GR, including EC. The methodology disaggregated and assessed the effects of EC and energy on GR in terms of GR’s proportion of EC, six EI categories, and the carbon reduction impacts. The analysis revealed that GR’s EC accounted for 10.6%, reducing to 9.89% when EPD materials were used. In terms of the reduction impact across six EIs, GWP was reduced to 0.84 and EP to 0.96. However, ODP, ADP, AP, and POCP, all elevated by high EIs from material inputs, increased to 626.7, 1.04, 1.16, and 250.09, respectively. Ultimately, the carbon reduction in GR was 24.9% when considering only energy usage, and 16.1% when including EC. When EPD materials were applied, the efficiency of reduction improved by an additional 0.6%, indicating a minimal application effect. Based on these findings, the differences in GR’s EC compared to new constructions, reduction limitations, and potential improvements were discussed. Full article

This study aims to examine the spatiotemporal variations of extreme rainfall events in Seoul, South Korea, utilizing subhourly rainfall data collected from an extensive observation network. Employing the Mann–Kendall trend detection test and Moran’s I index, this study analyzed temporal trends and spatial distributions in the extreme rainfall time series at 37 weather stations. The findings revealed that the annual maximum precipitation (AMP) series for various durations in Seoul generally exhibited a decreasing trend, with a marked decline observed for durations of 300 and 360 min. Furthermore, the study identified notable changes in the spatial patterns of AMP events, particularly for subhourly AMP events. The results indicate an intensification in the spatial concentration of AMP distribution. Consequently, despite the overall reduction in AMP, the likelihood of urban flooding may increase. This suggests that existing hydraulic design criteria, based on past concepts that temporal and spatial variability are constant, may no longer adequately represent extreme rainfall events. Therefore, revising these criteria to account for the evolving nature of extreme rainfall patterns is imperative. Full article

In this study, tin oxide (SnO₂)/polyaniline (PANI) composite nanowires (NWs) with varying amounts of PANI were synthesized for carbon dioxide (CO₂) gas sensing at room temperature (RT, 25 °C). SnO₂ NWs were fabricated via the vapor–liquid–solid (VLS) method, followed by coating with PANI. CO₂ sensing investigations revealed that the sensor with 186 μL PANI exhibited the highest response to CO₂ at RT. Additionally, the optimized sensor demonstrated excellent selectivity for CO₂, long-term stability, and reliable performance across different humidity levels. The enhanced sensing performance of the optimized sensor was attributed to the formation of SnO₂-PANI heterojunctions and the optimal PANI concentration. This study underscores the potential of SnO₂-PANI composites for CO₂ detection at RT. Full article

Background/Objectives. Men experience Leydig cell and mitochondrial dysfunction due to the accumulation of reactive oxygen species during aging, leading to hormonal imbalances in the body. This results in symptoms of testosterone deficiency syndrome (TDS) as testosterone levels decline. Consequently, there is a growing need for alternative therapies, such as phytotherapy, to regulate testosterone secretion. Methods. In this study, we evaluated the potential of elderberry extract powder (KSB191) as a functional ingredient for improving TDS by analyzing its mechanism in regulating testosterone imbalance. The major compounds of KSB191 were rutin and fructose–leucine, and the efficacy of KSB191 was confirmed by observing increases in total testosterone, free testosterone, and sperm motility in an aged rat model with decreased testosterone levels. Additionally, we assessed safety by analyzing levels of prostate-specific antigen, alanine aminotransferase, aspartate aminotransferase, and creatinine. Results. To confirm the effectiveness of KSB191 in increasing testosterone synthesis and inhibiting its breakdown, we analyzed the expression levels of genes related to testosterone synthesis and degradation in the testis tissue. KSB191 not only increases the expression levels of enzymes (3β-HSD, CYP17A1, and 17β-HSD) that catalyze testosterone synthesis in Leydig cells, but also reduces the expression of enzymes (5α-reductase and CYP19A1) that degrade testosterone, thereby enhancing testosterone production in the body. Conclusions. KSB191 is predicted to be a novel functional ingredient that acts on Leydig cells and increases testosterone synthesis (particularly, the increase in free testosterone), ultimately alleviating the symptoms of TDS. Full article

As we move towards the commercialization and upscaling of perovskite solar cells, it is essential to fabricate them in ambient environment rather than in the conventional glove box environment. The efficiency of ambient-processed perovskite solar cells lags behind those fabricated in controlled environments, primarily owing to external environmental factors such as humidity and temperature. In the case of device fabrication in ambient environments, relying solely on a single parameter, such as temperature or humidity, is insufficient for accurately characterizing environmental conditions. Therefore, the dew point is introduced as a parameter which accounts for both temperature and humidity. In this study, a machine learning model was developed to predict the efficiency of ambient-processed perovskite solar cells based on meteorological data, particularly the dew point. A total of 238 perovskite solar cells were fabricated, and their photovoltaic parameters and dew points were collected from March to December 2023. The collected data were used to train various tree-based machine learning models, with the random forest model achieving the highest accuracy. The efficiencies of the perovskite solar cells fabricated in January and February 2024 were predicted with a MAPE of 4.44%. An additional Shapley Additive exPlanations analysis confirmed the significance of the dew point in the performance of perovskite solar cells. Full article

Background/objective: Older patients from long-term care hospitals (LTCHs) presenting to emergency departments (EDs) exhibit a higher prevalence of frailty than those from the community. However, no study has examined frailty in patients from LTCHs in the ED. This study compared frailty in older patients from LTCHs and the community. Methods: We retrospectively analyzed data from the EDs of three university hospitals between 1 August and 31 October 2023, involving 5908 patients (515 from LTCHs and 5393 from the community). The Korean version of the Clinical Frailty Scale (CFS-K) was used to assess individuals aged 65 and older. We compared clinical characteristics, frailty, length of stay (LOS), and diagnosis between patients from LTCHs (LTCH group) and the community (community group). Results: Among ED patients, 55.0% and 35.2% in the LTCH and the community groups, respectively, were frail (p < 0.001). Of these, 71.7% in the LTCH group were hospitalized compared with 53.1% in the community group (p = 0.001). The odds ratio for in-hospital mortality was 4.910 (95% CI 1.458–16.534, p = 0.010) for frail LTCH patients and 3.748 (95% CI 2.599–5.405, p < 0.001) for frail community patients, compared to non-frail patients. Conclusions: Patients from LTCHs with frailty had higher hospital admission rates and increased in-hospital mortality compared to those in the community at the same frailty level. This study offers essential insights into the characteristics of older patients in LTCHs for healthcare administrators and medical staff worldwide. Full article

In this study, a 4D trajectory weather (4DT-Wx) prototype system was developed and evaluated for effective weather information integration in trajectory-based operation (TBO) environments. The system has two key distinguishing features: multi-model-based trajectory services and buffer zone information provision. We constructed a distributed processing system using Apache Spark, enabling the efficient processing of large-scale weather data. The performance evaluation demonstrated excellent scalability and efficiency in processing large-scale data. An analysis of the buffer configurations highlighted that buffer zone information is valuable in decision-making processes and has the potential to enhance the system performance. The system’s practical applicability is presented through visualizations of the extracted weather information. This system is expected to enhance aviation safety and operational efficiency, providing a foundation for addressing increasingly complex weather conditions and flight scenarios in the future. The approach presented in this study marks a significant step toward effective TBO implementation and the advancement of future air traffic management. The evaluation of the 4DT-Wx system analyzed the accuracy of weather data processing and the performance of distributed processing, finding that the temperature (T) estimation had the highest accuracy, and that the parallel processing using Apache Spark was most effectively modeled by Ahmed et al.’s model. The findings suggest the potential for further optimization in integrating various weather models and developing algorithms to enhance their utilization. Full article