Special Issue "Field Methods for Water Quality Surveying"

A special issue of Water (ISSN 2073-4441). This special issue belongs to the section "Water Quality and Contamination".

Deadline for manuscript submissions: closed (30 September 2022) | Viewed by 10857

Special Issue Editors

School of Engineering, Newcastle University, Newcastle, UK
Interests: environmental engineering; environmental chemistry; environmental pollution risk assessment and remediation; water quality and public health”
Special Issues, Collections and Topics in MDPI journals
School of Civil Engineering and Geosciences, Newcastle University, Newcastle, UK
Interests: molecular biology; biodegradation; environmental science; sustainable development; water quality and public health
School of Water, Energy and Environment, Cranfield University, Cranfield, UK
Interests: paper microfluidics sensors; wastewater-based epidemiology; biosensors; water quality; infection diagnosis; public health

Special Issue Information

Dear Colleagues,

There is breathtaking progress in the development of field methods for water quality surveying, from portable next generation sequencing devices for on-site characterization of water microbiomes to novel sensors for real-time monitoring of waterborne hazards. These methods facilitate monitoring of water quality in treatment works and remote locations, avoid sample alteration during transportation and storage, and enable more rapid data exploitation for immediate decision making. Miniaturized equipment items enable cutting-edge water quality monitoring in low-resource settings, “where there is no laboratory”. Robust field kits for water quality assessments help citizen scientists participate in environmental research. Rapid and online water quality senors provide enormous water quality data sets with unprecedented spatiotemporal resolution at the catchment scale. This rapid technology development brings new scientific challenges in analytical quality control and assurance, big data management, and interpretation for decision making. For this special issue we invite contributions reporting advancements in the state of the art of field deployable methods for water quality surveying: exemplary field studies, method validations, digital applications for field data collection, transmission, management, and sharing, and novel water sensor developments and their applications. We invite reports of excellent quality water quality research which aligns with the special issue motto: Out of the lab, into the field!

Prof. David Werner
Dr. Kishor Acharya
Dr. Zhugen Yang
Guest Editors

Manuscript Submission Information

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Keywords

  • Portable devices for water quality testing
  • Mobile water quality laboratories
  • Novel sensors for online water quality monitoring
  • Case studies of on-site and in-situ water quality surveys
  • Intelligent water quality sensing and communications
  • Digital applications to support field data collection, management and sharing
  • Citizen science for water quality
  • Point-of-use water sensors

Published Papers (4 papers)

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Research

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Article
Citizen Science for Water Quality Monitoring in the Meki River, Ethiopia: Quality Assurance and Comparison with Conventional Methods
Water 2023, 15(2), 238; https://doi.org/10.3390/w15020238 - 05 Jan 2023
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Abstract
A lack of water quality information for many water bodies around the world makes it difficult to identify global change and discover early signs of myriad threats to freshwater resources. This problem is widely seen in Ethiopia due to absence of regular monitoring. [...] Read more.
A lack of water quality information for many water bodies around the world makes it difficult to identify global change and discover early signs of myriad threats to freshwater resources. This problem is widely seen in Ethiopia due to absence of regular monitoring. Citizen science has a great potential to fill these gaps in water quality data, but there is concern about the accuracy of data collected by citizen scientists. Moreover, there is a gap to engage citizen scientists in water quality monitoring, and there is still insufficient awareness of how citizen scientists can become part of a collaborative scheme. This study aimed to evaluate the accuracy of water quality collected by citizen scientists and characterize the water quality of the Meki River with the involvement of citizen scientists. The suitability of the river water for irrigation was evaluated using a combination of citizen science and conventional water quality data collection methods. Water temperature, turbidity, ammonia, phosphate, nitrate, nitrite, total alkalinity, total hardness, and pH were analyzed by both citizen scientists and in a conventional laboratory. The citizen scientists’ data, expressed as percent of synthetic standard solution concentrations, indicated good agreement for selected water quality parameters: 123.8 ± 24.7% for PO43−, 115.6 ± 6.3% for NO3, 105.8 ± 7.4% for pH, and 133.3 ± 23.6% for NH4+. Thus, citizen scientists can monitor and collect water quality data accurately. From the results, the Meki River water can be used for irrigation, but pollution sources should be controlled to reduce further quality deterioration as the population increases. Full article
(This article belongs to the Special Issue Field Methods for Water Quality Surveying)
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Article
A Mobile Laboratory Enables Fecal Pollution Source Tracking in Catchments Using Onsite qPCR Assays
Water 2022, 14(8), 1224; https://doi.org/10.3390/w14081224 - 11 Apr 2022
Cited by 2 | Viewed by 2258
Abstract
Onsite molecular diagnostics can revolutionize fecal pollution source tracking. We aimed to validate a method for onsite qPCR assays with a miniature speaker-sized Q qPCR instrument and other portable equipment items. We showed that marker genes for total bacteria (16S) and E. coli [...] Read more.
Onsite molecular diagnostics can revolutionize fecal pollution source tracking. We aimed to validate a method for onsite qPCR assays with a miniature speaker-sized Q qPCR instrument and other portable equipment items. We showed that marker genes for total bacteria (16S) and E. coli (rodA) in 100 mL of river water measured with this method agreed within ±0.3 log10 units with results obtained when using conventional laboratory equipment items. We then deployed the portable method in a mobile laboratory (‘lab in a van’) and quantified HF183 marker genes for human host associated Bacteroides in river water within 3 h of sampling. We also used the mobile laboratory to investigate urban river water and effluents from two storm drains and a retention pond and collected comprehensive microbial and physicochemical water quality data. We found significantly higher HF183 gene levels in the older storm drain compared to the river water (6.03 ± 0.04 vs. 4.23 ± 0.03 log10 gene copies per 100 mL), and a principal component analysis revealed that storm drain effluent retention in a pond beneficially altered water characteristics, making them more like those of the receiving river. In conclusion, onsite qPCR assays can be performed with portable equipment items to quickly test water. Full article
(This article belongs to the Special Issue Field Methods for Water Quality Surveying)
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Article
Performance of a Handheld Chlorophyll-a Fluorometer: Potential Use for Rapid Algae Monitoring
Water 2021, 13(10), 1409; https://doi.org/10.3390/w13101409 - 18 May 2021
Cited by 2 | Viewed by 2259
Abstract
Chlorophyll-a measurements are an important factor in the water quality monitoring of surface waters, especially for determining the trophic status and ecosystem management. However, a collection of field samples for extractive analysis in a laboratory may not fully represent the field conditions. [...] Read more.
Chlorophyll-a measurements are an important factor in the water quality monitoring of surface waters, especially for determining the trophic status and ecosystem management. However, a collection of field samples for extractive analysis in a laboratory may not fully represent the field conditions. Handheld fluorometers that can measure chlorophyll-a in situ are available, but their performance in waters with a variety of potential light-interfering substances has not yet been tested. We tested a handheld fluorometer for sensitivity to ambient light and turbidity and compared these findings with EPA Method 445.0 using water samples obtained from two urban lakes in Tucson, Arizona, USA. Our results suggested that the probe was not sensitive to ambient light and performed well at low chlorophyll-a concentrations (<25 µg/L) across a range of turbidity levels (50–70 NTU). However, the performance was lower when the chlorophyll-a concentrations were >25 µg/L and turbidity levels were <50 NTU. To account for this discrepancy, we developed a calibration equation to use for this handheld fluorometer when field monitoring for potential harmful algal blooms in water bodies. Full article
(This article belongs to the Special Issue Field Methods for Water Quality Surveying)
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Review

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Review
MinION Nanopore Sequencing Accelerates Progress towards Ubiquitous Genetics in Water Research
Water 2022, 14(16), 2491; https://doi.org/10.3390/w14162491 - 12 Aug 2022
Cited by 2 | Viewed by 4711
Abstract
In 2014, Oxford Nanopore Technologies (ONT) introduced an affordable and portable sequencer called MinION. We reviewed emerging applications in water research and assessed progress made with this platform towards ubiquitous genetics. With >99% savings in upfront costs as compared to conventional platforms, the [...] Read more.
In 2014, Oxford Nanopore Technologies (ONT) introduced an affordable and portable sequencer called MinION. We reviewed emerging applications in water research and assessed progress made with this platform towards ubiquitous genetics. With >99% savings in upfront costs as compared to conventional platforms, the MinION put sequencing capacity into the hands of many researchers and enabled novel applications with diverse remits, including in countries without universal access to safe water and sanitation. However, to realize the MinION’s fabled portability, all the auxiliary equipment items for biomass concentration, genetic material extraction, cleanup, quantification, and sequencing library preparation also need to be lightweight and affordable. Only a few studies demonstrated fully portable workflows by using the MinION onboard a diving vessel, an oceanographic research ship, and at sewage treatment works. Lower nanopore sequencing read accuracy as compared to alternative platforms currently hinders MinION applications beyond research, and inclusion of positive and negative controls should become standard practice. ONT’s EPI2ME platform is a major step towards user-friendly bioinformatics. However, no consensus has yet emerged regarding the most appropriate bioinformatic pipeline, which hinders intercomparison of study results. Processing, storing, and interpreting large data sets remains a major challenge for ubiquitous genetics and democratizing sequencing applications. Full article
(This article belongs to the Special Issue Field Methods for Water Quality Surveying)
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