Special Issue "Voyages through the Multiple Scales of Virus Biology"

A special issue of Viruses (ISSN 1999-4915). This special issue belongs to the section "General Virology".

Deadline for manuscript submissions: 31 May 2021.

Special Issue Editor

Dr. Fasséli Coulibaly
E-Mail Website
Guest Editor
Department of Biochemistry & Molecular Biology, Infection & Immunity program, Biomedicine Discovery Institute, Monash University, Melbourne, Australia
Interests: structural virology; insect viruses; poxviruses; nanotechnology; vaccines

Special Issue Information

Dear colleagues,

Viruses are only truly active when they interact with and infect a cell. As a consequence, a key pursuit of Molecular and Structural Virology has been to provide a molecular understanding of viral processes in the context of the cellular environment. Exciting methodological developments have made significant headway in this direction allowing the in situ analysis of native viral components with high spatiotemporal resolution.

To reflect these advances, the journal Viruses has designed a special issue accepting contributions with an emphasis on biophysics, imaging and structural biology, and their integration to provide a view of the virus world at multiple scales.

Not unlike J. Swift’s character Gulliver, we invite contributions that feature viruses either as giant molecular machines achieving biochemical wonders or as masterful dwarfs in the vast environment of the cell, and perhaps both at the same time.

Dr. Fasséli Coulibaly
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

Structural Virology; In situ analysis; Multiscale analysis; High-resolution and live imaging; X-ray crystallography; Cryo-electron microscopy; Tomography; Biophysics; Mathematical modelling; Giant viruses; Methods

Published Papers (1 paper)

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Research

Open AccessArticle
Light Sheet Microscopy-Assisted 3D Analysis of SARS-CoV-2 Infection in the Respiratory Tract of the Ferret Model
Viruses 2021, 13(3), 529; https://doi.org/10.3390/v13030529 - 23 Mar 2021
Viewed by 603
Abstract
The visualization of viral pathogens in infected tissues is an invaluable tool to understand spatial virus distribution, localization, and cell tropism in vivo. Commonly, virus-infected tissues are analyzed using conventional immunohistochemistry in paraffin-embedded thin sections. Here, we demonstrate the utility of volumetric three-dimensional [...] Read more.
The visualization of viral pathogens in infected tissues is an invaluable tool to understand spatial virus distribution, localization, and cell tropism in vivo. Commonly, virus-infected tissues are analyzed using conventional immunohistochemistry in paraffin-embedded thin sections. Here, we demonstrate the utility of volumetric three-dimensional (3D) immunofluorescence imaging using tissue optical clearing and light sheet microscopy to investigate host–pathogen interactions of pandemic SARS-CoV-2 in ferrets at a mesoscopic scale. The superior spatial context of large, intact samples (>150 mm3) allowed detailed quantification of interrelated parameters like focus-to-focus distance or SARS-CoV-2-infected area, facilitating an in-depth description of SARS-CoV-2 infection foci. Accordingly, we could confirm a preferential infection of the ferret upper respiratory tract by SARS-CoV-2 and suggest clustering of infection foci in close proximity. Conclusively, we present a proof-of-concept study for investigating critically important respiratory pathogens in their spatial tissue morphology and demonstrate the first specific 3D visualization of SARS-CoV-2 infection. Full article
(This article belongs to the Special Issue Voyages through the Multiple Scales of Virus Biology)
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