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Veterinary Sciences
Special Issues
Advances in Animal Reproductive Biology and Technologies
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Advances in Animal Reproductive Biology and Technologies

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Reproduction and Obstetrics".

Deadline for manuscript submissions: 31 August 2026 | Viewed by 2125

Special Issue Editors

Prof. Dr. Qing Yang

E-Mail Website
Guest Editor
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
Interests: reproductive physiology and mechanisms of reproductive disorders in livestock
Prof. Dr. Jiaojiao Zhang

E-Mail Website
Guest Editor
Chongqing Key Laboratory of Forage and Herbivore, College of Veterinary Medicine, Southwest University, Chongqing 400715, China
Interests: regulation of animal reproductive physiology

Special Issue Information

Dear Colleagues,

This Special Issue delves into the physiological mechanisms of reproduction and the underlying causes of reproductive disorders in animals. It presents the latest research on the hormonal regulation of reproduction, gametogenesis, fertilization, and embryo development, shedding light on how these processes can be optimized for improved fertility. The Issue also addresses common reproductive disorders in animals, including ovarian dysfunction, endometritis, and male infertility, exploring their pathophysiology and clinical management. Advanced diagnostic tools, such as genomic and proteomic profiling, are discussed as key technologies in identifying biomarkers for early detection and treatment of reproductive health issues. Moreover, innovative therapies aimed at correcting hormonal imbalances and enhancing reproductive efficiency are examined, offering new solutions for managing infertility and improving breeding outcomes. This collection provides an in-depth understanding of the complex mechanisms driving reproductive health in animals and the clinical interventions that can mitigate reproductive disorders, ensuring sustainable and productive animal husbandry.

Prof. Dr. Qing Yang
Prof. Dr. Jiaojiao Zhang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Veterinary Sciences is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • animal reproduction
  • assisted reproductive technologies
  • in vitro fertilization
  • cryopreservation
  • embryo manipulation
  • reproductive physiology
  • hormonal regulation
  • animal fertility
  • ovarian dysfunction
  • embryonic development
  • reproductive toxicity
  • reproductive endocrinology
  • reproductive disorders

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

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26 pages, 5646 KB  
Article
Study on Early Pregnancy Diagnosis of Sows Based on Body Fluid Metabolite Detection Combined with Machine Learning Models
by Yun Feng, Ruonan Gao, Wengang Yang, Huiwen Lu, Weizeng Sun, Yun Zhang, Yujun Ren, Liming Gao, Mengxun Li, Qingchun Li, Guang Pu, Yongsheng Zhang, Zikai Ai, Kun Yan and Tao Huang
Vet. Sci. 2026, 13(5), 409; https://doi.org/10.3390/vetsci13050409 - 22 Apr 2026
Viewed by 470
Abstract
The conventional window for ultrasonic pregnancy diagnosis in sows is 22–25 days post-insemination, which often results in missed opportunities for the optimal re-insemination of non-pregnant sows and elevated production costs. This present study aimed to establish an early pregnancy detection method for sows [...] Read more.
The conventional window for ultrasonic pregnancy diagnosis in sows is 22–25 days post-insemination, which often results in missed opportunities for the optimal re-insemination of non-pregnant sows and elevated production costs. This present study aimed to establish an early pregnancy detection method for sows at 12–18 days post-insemination, thereby providing a reference for efficient reproductive management. Saliva, urine and vaginal secretions were collected from sows during this period, and seven metabolites were quantified. Seven machine learning models were employed for data analysis, after which the optimal combination was determined, and the detection protocol was refined using recursive feature elimination. The results revealed that the majority of metabolites in saliva and urine differed significantly between pregnant and non-pregnant groups (p < 0.05). Among the models evaluated, the random forest algorithm exhibited the best predictive performance, with accuracy ranging from 0.59 to 1.00. Saliva sampled at 17 days post-insemination was identified as the optimal diagnostic medium, and 100% prediction accuracy was achieved by measuring only three metabolites: Glc, Ste, and Xan. The diagnostic approach established in this study allows pregnancy detection 5–8 days earlier than conventional methods, with the additional benefits of non-invasive sampling and minimal stress to sows. Accordingly, it provides a novel reference for enhancing the efficiency of swine production. Full article
(This article belongs to the Special Issue Advances in Animal Reproductive Biology and Technologies)
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12 pages, 1280 KB  
Article
Administration of Human Chorionic Gonadotropin Combined with Phenylbutazone at the Time of Embryo Transfer Synergistically Improves Pregnancy Rates in Dromedary Camels
by Mahmoud Moussa, Salahaddin Ahmed, Mohamed Elbaz and Kamaal Pasha
Vet. Sci. 2026, 13(1), 85; https://doi.org/10.3390/vetsci13010085 - 15 Jan 2026
Viewed by 859
Abstract
Effective luteal support is crucial for successful pregnancy following embryo transfer in dromedary camels, highlighting the importance of maintaining adequate luteal function. This study compared three luteal support protocols for pregnancy rates after embryo transfer (ET) in dromedary camels. Recipients with a single [...] Read more.
Effective luteal support is crucial for successful pregnancy following embryo transfer in dromedary camels, highlighting the importance of maintaining adequate luteal function. This study compared three luteal support protocols for pregnancy rates after embryo transfer (ET) in dromedary camels. Recipients with a single ovarian follicle (14~16 mm) were assigned into: (1) untreated control, (2) Phenylbutazone (PBZ) at 3.7 mg/kg IV prior to ET, or (3) human chorionic gonadotropin (hCG) 2000 IU plus 3.7 mg/kg IV PBZ prior to ET. Recipients that showed ovarian inactivity were treated with an injection of 1800 IU of equine chorionic gonadotropin (eCG) after progesterone (P4) priming. Pregnancy was assessed on days 10 (P4 assay) and 60 (transrectal ultrasonography) post-ET. Pregnancy rates on day 10 were higher in the hCG + PBZ (73.3%) and eCG (68.0%) groups than in the PBZ (44.0%) and control (35.0%) groups (p < 0.05). By day 60, the hCG + PBZ group exhibited the highest pregnancy maintenance rate (81.8%) and the lowest embryonic loss rate (18.2%), compared with eCG (53.0%; 47.0%), PBZ (54.5%; 45.5%), and control (57.1%; 42.9%) groups, respectively (p < 0.05). However, there was no significant difference among the eCG, PBZ, and control groups in embryonic loss rates. In conclusion, hCG synergizes with PBZ to enhance corpus luteum function, uterine receptivity, and pregnancy maintenance more effectively than PBZ or eCG. Full article
(This article belongs to the Special Issue Advances in Animal Reproductive Biology and Technologies)
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Review

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16 pages, 1403 KB  
Review
Application of Anti-Müllerian Hormone in Regulation of Ovarian Function and Its Practical Relevance for Fertility and Embryo Production in Cows and Mares
by Andreas Vernunft and Dragos Scarlet
Vet. Sci. 2026, 13(6), 552; https://doi.org/10.3390/vetsci13060552 - 3 Jun 2026
Viewed by 369
Abstract
In the female organism, Anti-Müllerian hormone (AMH) is produced exclusively by granulosa cells, particularly in secondary and early tertiary follicles. The amount of AMH released into the blood is proportional to the total number of follicles present in the ovaries and is therefore [...] Read more.
In the female organism, Anti-Müllerian hormone (AMH) is produced exclusively by granulosa cells, particularly in secondary and early tertiary follicles. The amount of AMH released into the blood is proportional to the total number of follicles present in the ovaries and is therefore used to estimate the ovarian functional reserve. In both cattle and mares, a strong and highly repeatable association has also been demonstrated between AMH concentrations and antral follicle count. Therefore, AMH may serve as a biomarker with broad diagnostic utility in reproductive medicine in these species, as it reflects the functional ovarian reserve through its association with viable antral and preantral follicles. While AMH is already being used for clinical diagnostics in human reproductive medicine and has been extensively investigated, it has received little attention in applied veterinary practice so far. This review explores the applications of AMH for reproductive medicine in cattle and horses, monoovulatory farm animals in which assisted reproductive technologies are widely used. The objectives of this review are to (I) provide clinically relevant background information and a comparative discussion of (II) the role of AMH in follicular development, (III) its value as a fertility indicator, and (IV) its significance for embryo production in vivo and in vitro in cows and mares, with particular emphasis on practical relevance and potential applications. Full article
(This article belongs to the Special Issue Advances in Animal Reproductive Biology and Technologies)
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