Trends and Recent Advances in Capillary Electrophoresis

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (29 January 2021) | Viewed by 4541

Special Issue Editor


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Guest Editor
Institut des Biomolécules Max Mousseron, UMR CNRS 5247, Université de Montpellier, Place Eugène Bataillon, case courrier 1706, F-34095 Montpellier Cedex 5, France
Interests: capillary electrophoresis; Taylor dispersion analysis; molecular diffusion; weak biomolecular interactions; microemulsion; hydrodynamic radius

Special Issue Information

Dear Colleagues,

Capillary electrophoresis (CE) was shown to be a major analytical separation technique for the analysis of both small and large (polymers, proteins, etc.) molecules, where its performance is often better or complementary to other liquid chromatography techniques. Different modes of CE exist (CZE, FACE, ACE, ITP, etc.) and each one allows scientists not only to separate and quantify the analytes but also to quantify other physico-chemical parameters such as association constants, effective charge, etc.

The scope of this Special Issue is broad and includes all aspects of capillary electrophoresis. It aims to present the most significant and recent applications of CE.

Dr. Joseph Chamieh
Guest Editor

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Keywords

  • Capillary electrophoresis
  • Weak interactions
  • Effective charge
  • Capillary coatings
  • Frontal analysis
  • Isotachophoresis
  • Affinity capillary electrophoresis

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Published Papers (1 paper)

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Research

9 pages, 1791 KiB  
Article
Single-Run Separation and Quantification of 14 Cannabinoids Using Capillary Electrophoresis
by Emil A. Zaripov, Tiah Lee, Yuchu Dou, Cory S. Harris, Artem Egorov and Maxim V. Berezovski
Separations 2021, 8(3), 30; https://doi.org/10.3390/separations8030030 - 12 Mar 2021
Cited by 6 | Viewed by 3941
Abstract
Quantification of major cannabinoids in cannabis products is normally performed using high-pressure liquid chromatography (HPLC)-based methods. We propose a cost-effective alternative method that successfully separates and quantifies 14 cannabinoids in a single run using capillary electrophoresis (CE) coupled with a UV detector in [...] Read more.
Quantification of major cannabinoids in cannabis products is normally performed using high-pressure liquid chromatography (HPLC)-based methods. We propose a cost-effective alternative method that successfully separates and quantifies 14 cannabinoids in a single run using capillary electrophoresis (CE) coupled with a UV detector in 18 min. The separation is carried out in 60% acetonitrile in the presence of 6.5 mM sodium hydroxide and 25 µM β-cyclodextrin, resulting in good separation of cannabinoids. Our CE method demonstrated the limit of detection between 1.2–1.8 µg/mL, with the linear range reaching up to 50 µg/mL. We validated the method performance by testing a plant extract and quantifying cannabinoid content. This method is the first to separate 14 cannabinoids in one run using a CE system with UV detection. Full article
(This article belongs to the Special Issue Trends and Recent Advances in Capillary Electrophoresis)
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