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Plants
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Molecular Mechanisms Involved in Somatic Embryogenesis and Organogenesis of Plants
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Molecular Mechanisms Involved in Somatic Embryogenesis and Organogenesis of Plants

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: 31 May 2025 | Viewed by 5665

Special Issue Editor

Dr. Cabrera-Ponce José Luis

E-Mail Website
Guest Editor
CINVESTAV-IPN, Departamento de Ingenieria Genetica, Unidad Irapuato, Irapuato, Mexico
Interests: plant regeneration; somatic embryogenesis; gene regulatory networks; transcriptomic analysis; genetic transformation; genome editing

Special Issue Information

Dear Colleagues,

The natural ability of plants to regenerate has been the fundamental basis for several agricultural and biotechnological approaches.

Biotechnologists extended this ability using plant growth regulators (auxin and cytokinins) coupled with plant tissue culture media and stress signaling (light, osmotic, saline, heavy metal and temperature stress). This has promoted the manipulation of many plant species that regenerate by somatic embryogenesis and organogenesis processes.

However, many important species exhibit recalcitrance, meaning that the current protocols are unsuccessful for them; this is one of the major bottlenecks facing the pharmacology industry, micropropagation and plant genetic engineering.

In the genomic era, the application of omic technologies (transcriptomic, proteomic and metabolomic) will help us to elucidate the fundamental processes of growth and development.

This Special Issue will cover various topics, with the aim of contributing to current knowledge on plant regeneration mediated by somatic embryogenesis and organogenesis.

Topics that will be considered for this Special Issue include:

  • Transcriptomic, proteomic and metabolomic analysis applied to plant regeneration.
  • Gene regulatory networks.
  • Genetic transformation and genome editing to increase the efficiency of plant regeneration.

We also encourage the submission of confirmatory findings and negative or inconclusive results which address rigorously tested hypotheses about the regeneration of recalcitrant plant species.

Dr. Cabrera-Ponce José Luis
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plants
  • somatic embryogenesis
  • organogenesis
  • omic technologies
  • genetic transformation
  • genome editing
  • plant growth regulators

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

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21 pages, 14187 KiB  
Article
Functional Analysis of the PoSERK-Interacting Protein PorbcL in the Embryogenic Callus Formation of Tree Peony (Paeonia ostii T. Hong et J. X. Zhang)
by Yinglong Song, Jiange Wang, Jiale Zhu, Wenqian Shang, Wenqing Jia, Yuke Sun, Songlin He, Xitian Yang and Zheng Wang
Plants 2024, 13(19), 2697; https://doi.org/10.3390/plants13192697 - 26 Sep 2024
Cited by 1 | Viewed by 943
Abstract
SERK is a marker gene for early somatic embryogenesis. We screened and functionally verified a SERK-interacting protein to gain insights into tree-peony somatic embryogenesis. Using PoSERK as bait, we identified PorbcL (i.e., the large subunit of Rubisco) as a SERK-interacting protein from a [...] Read more.
SERK is a marker gene for early somatic embryogenesis. We screened and functionally verified a SERK-interacting protein to gain insights into tree-peony somatic embryogenesis. Using PoSERK as bait, we identified PorbcL (i.e., the large subunit of Rubisco) as a SERK-interacting protein from a yeast two-hybrid (Y2H) library of cDNA from developing tree-peony somatic embryos. The interaction between PorbcL and PoSERK was verified by Y2H and bimolecular fluorescence complementation analyses. PorbcL encodes a 586-amino-acid acidic non-secreted hydrophobic non-transmembrane protein that is mainly localized in the chloroplast and plasma membrane. PorbcL was highly expressed in tree-peony roots and flowers and was up-regulated during zygotic embryo development. PorbcL overexpression caused the up-regulation of PoSERK (encoding somatic embryogenesis receptor-like kinase), PoAGL15 (encoding agamous-like 15), and PoGPT1 (encoding glucose-6-phosphate translocator), while it caused the down-regulation of PoLEC1 (encoding leafy cotyledon 1) in tree-peony callus. PorbcL overexpression led to increased indole-3-acetic acid (IAA) content but decreasing contents of abscisic acid (ABA) and 6-benzyladenosine (BAPR). The changes in gene expression, high IAA levels, and increased ratio of IAA to ABA, BAPR, 1-Aminocyclopropanecarboxylic acid (ACC), 5-Deoxystrigol (5DS), and brassinolide (BL) promoted embryogenesis. These results provide a foundation for establishing a tree-peony embryogenic callus system. Full article
(This article belongs to the Special Issue Molecular Mechanisms Involved in Somatic Embryogenesis and Organogenesis of Plants)
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13 pages, 5070 KiB  
Article
Molecular Insights into the Role of Sterols in Microtuber Development of Potato Solanum tuberosum L.
by Lisset Herrera-Isidron, Eliana Valencia-Lozano, Braulio Uribe-Lopez, John Paul Délano-Frier, Aarón Barraza and José Luis Cabrera-Ponce
Plants 2024, 13(17), 2391; https://doi.org/10.3390/plants13172391 - 27 Aug 2024
Cited by 2 | Viewed by 1391
Abstract
Potato tubers are reproductive and storage organs, enabling their survival. Unraveling the molecular mechanisms that regulate tuberization is crucial for understanding how potatorespond to environmental stress situations and for potato breeding. Previously, we did a transcriptomic analysis of potato microtuberization without light. This [...] Read more.
Potato tubers are reproductive and storage organs, enabling their survival. Unraveling the molecular mechanisms that regulate tuberization is crucial for understanding how potatorespond to environmental stress situations and for potato breeding. Previously, we did a transcriptomic analysis of potato microtuberization without light. This showed that important cellular processes like ribosomal proteins, cell cycle, carbon metabolism, oxidative stress, fatty acids, and phytosterols (PS) biosynthesis were closely connected in a protein–protein interaction (PPI) network. Research on PS function during potato tuberization has been scarce. PS plays a critical role in regulating membrane permeability and fluidity, and they are biosynthetic precursors of brassinosteroids (BRs) in plants, which are critical in regulating gene expression, cell division, differentiation, and reproductive biology. Within a PPI network, we found a module of 15 genes involved in the PS biosynthetic process. Darkness, as expected, activated the mevalonate (MVA) pathway. There was a tight interaction between three coding gene products for HMGR3, MVD2, and FPS1, and the gene products that synthetize PS, including CAS1, SMO1, BETAHSD, CPI1, CYP51, FACKEL, HYDRA1, SMT2, SMO2, STE1, and SSR1. Quantitative real-time polymerase chain reaction (qRT-PCR) confirmed the expression analysis of ten specific genes involved in the biosynthesis of PS. This manuscript discusses the potential role of genes involved in PS biosynthesis during microtuber development. Full article
(This article belongs to the Special Issue Molecular Mechanisms Involved in Somatic Embryogenesis and Organogenesis of Plants)
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Review

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28 pages, 2364 KiB  
Review
Optimizing Brassica oleracea L. Breeding Through Somatic Hybridization Using Cytoplasmic Male Sterility (CMS) Lines: From Protoplast Isolation to Plantlet Regeneration
by Miriam Romero-Muñoz and Margarita Pérez-Jiménez
Plants 2024, 13(22), 3247; https://doi.org/10.3390/plants13223247 - 19 Nov 2024
Viewed by 2560
Abstract
The Brassica oleracea L. species embrace important horticultural crops, such as broccoli, cauliflower, and cabbage, which are highly valued for their beneficial nutritional effects. However, the complexity of flower emasculation in these species has forced breeders to adopt biotechnological approaches such as somatic [...] Read more.
The Brassica oleracea L. species embrace important horticultural crops, such as broccoli, cauliflower, and cabbage, which are highly valued for their beneficial nutritional effects. However, the complexity of flower emasculation in these species has forced breeders to adopt biotechnological approaches such as somatic hybridization to ease hybrid seed production. Protoplasts entail a versatile tool in plant biotechnology, supporting breeding strategies that involve genome editing and hybridization. This review discusses the use of somatic hybridization in B. oleracea L. as a biotechnological method for developing fusion products with desirable agronomic traits, particularly cytoplasmic male sterile (CMS) condition. These CMS lines are critical for implementing a cost-effective, efficient, and reliable system for producing F1 hybrids. We present recent studies on CMS systems in B. oleracea L. crops, providing an overview of established models that explain the mechanisms of CMS and fertility restoration. Additionally, we emphasize key insights gained from protoplast fusion applied to B. oleracea L. breeding. Key steps including pre-treatments of donor plants, the main tissues used as sources of parental protoplasts, methods for obtaining somatic hybrids and cybrids, and the importance of establishing a reliable plant regeneration method are discussed. Finally, the review explores the incorporation of genome editing technologies, such as CRISPR-Cas9, to introduce multiple agronomic traits in Brassica species. This combination of advanced biotechnological tools holds significant promise for enhancing B. oleracea breeding programs in the actual climate change context. Full article
(This article belongs to the Special Issue Molecular Mechanisms Involved in Somatic Embryogenesis and Organogenesis of Plants)
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