Super-Resolution Optical Microscopy: Science and Applications

A special issue of Photonics (ISSN 2304-6732). This special issue belongs to the section "New Applications Enabled by Photonics Technologies and Systems".

Deadline for manuscript submissions: 15 August 2026 | Viewed by 643

Special Issue Editors


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Guest Editor
School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian, China
Interests: single-molecule localization microscopy; super-resolution microscopy; adaptive optics; multicolor imaging; fluorescent dye
School of Optics and Photonics, Beijing Institute of Technology, Beijing, China
Interests: super-resolution imaging and intelligent computing; machine learning; bioinformatics; high-performance computing

Special Issue Information

Dear Colleagues,

Super-resolution microscopy stands as a pivotal advancement in biomedical research, enabling the visualization of biological structures beyond the diffraction limit of light. This breakthrough technology has revolutionized our ability to investigate subcellular architectures, molecular interactions, and dynamic processes with unprecedented spatial and temporal resolution. As a highly interdisciplinary field, super-resolution microscopy integrates principles from biology, chemistry, photonics, and computational science to uncover detailed morphological and functional information at the nanoscale.

This Special Issue, titled “Super-Resolution Optical Microscopy: Science and Applications”, invites the submission of high-quality basic, methodological, and applied research contributions, in the form of regular and review articles, focusing on

  • The development and validation of novel super-resolution microscopy techniques;
  • The study of photophysical and photochemical mechanisms underlying super-resolution, including probe design, labeling strategies, and optical manipulation;
  • The integration of multimodal, multispectral, or correlative approaches to enhance resolution, contrast, and functional insight;
  • The application and validation of super-resolution methods in preclinical and clinical settings, particularly in understanding pathological changes at the molecular level.

We welcome contributions that highlight both technological innovations and transformative biological or medical applications, aiming to bridge the gap between fundamental research and practical implementation in healthcare.

Dr. Donghan Ma
Dr. Fan Xu
Guest Editors

Manuscript Submission Information

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Keywords

  • super-resolution microscopy
  • single-molecule localization microscopy
  • structured illumination microscopy
  • stimulated emission depletion microscopy
  • DNA-PAINT
  • expansion microscopy
  • multimodal imaging

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Published Papers (1 paper)

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Research

17 pages, 10952 KB  
Article
Highly Integrated System Design for Wide-Field and High-Resolution Imaging with Ultra-Small-Pixel Sensor
by Zhiyu Zhang, Sibo Niu, Xue Dong, Meng Xiang, Yanyan Liu, Tong Zhang, Pingli Han and Fei Liu
Photonics 2025, 12(12), 1146; https://doi.org/10.3390/photonics12121146 - 21 Nov 2025
Viewed by 344
Abstract
This study introduces a novel imaging technology designed to address the critical challenges of high integration, wide field of view (FOV), and high-resolution detection in optoelectronic systems. The proposed approach leverages ultra-small pixels and a unique “single-eye + compound-eye” architecture, combining a concentric [...] Read more.
This study introduces a novel imaging technology designed to address the critical challenges of high integration, wide field of view (FOV), and high-resolution detection in optoelectronic systems. The proposed approach leverages ultra-small pixels and a unique “single-eye + compound-eye” architecture, combining a concentric spherical lens for wide-FOV light collection with a distributed camera array for segmented imaging and stitching of the Petzval image plane. This design enables high-resolution imaging across a large area while maintaining compact system dimensions. The ultra-small-pixel large-format detector excels in capturing fine details, and by applying linear system theory, the technology achieves significant reductions in system size and complexity without compromising performance. Experimental validation shows that the imaging system achieves a modulation transfer function near the diffraction limit at 500 lp/mm, with a root-mean-square spot size consistently below 1 μm. Additionally, the system delivers an angular resolution of 25 μrad and distortion-free imaging over a 61.5×55 FOV. Full article
(This article belongs to the Special Issue Super-Resolution Optical Microscopy: Science and Applications)
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