Advanced Quantitative Phase Microscopy: Techniques and Applications

A special issue of Photonics (ISSN 2304-6732). This special issue belongs to the section "Optical Interaction Science".

Deadline for manuscript submissions: closed (31 March 2025) | Viewed by 1728

Special Issue Editors


E-Mail Website
Guest Editor
School of Physics, Xidian University, Xi'an 710071, China
Interests: quantitative phase microscopy; optical diffraction tomography; laser interferometry; digital holography; multimodal microscopic imaging; organelle dynamics

E-Mail Website
Guest Editor
School of Optics and Photonics, Beijing Institute of Technology, Beijing 100081, China
Interests: quantitative phase imaging; computational microscopy; optical diffraction tomography; Fourier ptychography microscopy; phase retrieval

E-Mail
Guest Editor
School of Physics, Xidian University, Xi‘an 710071, China
Interests: super-resolution microscopy; single-molecule localization microscopy; structured illumination microscopy; quantitative phase contrast; digital holography

Special Issue Information

Dear Colleagues,

Quantitative phase microscopy (QPM) is an emerging label-free imaging technology that visualizes transparent samples by recovering the phase delay of the illumination beam caused when it passes through them. During the last decade, QPM has been widely used in industrial inspection, air/gas visualization, 3D imaging/display, and the biomedical field, etc. Meanwhile, many efforts have been made to enhance the spatial resolution, imaging speed, phase accuracy, and measurement range, as well as the reconstruction of QPM in general. Notably, with the rapid development of computer science and other subjects, several new quantitative phase imaging techniques have been developed, such as wavefront sensing, shearing interference, transport of intensity equation (TIE)-based approach, beam-propagation-based methods, Fourier ptychography, and so on. Meanwhile, dozens of image processing algorithms, including machine learning and deep learning technologies, have led to breakthroughs in QPM.

Topics of this Special Issue include, but are not limited to, the following:

  • Quantitative phase microscopy;
  • Phase retrieval;
  • Ptychography/Fourier ptychography;
  • Digital holographic microscopy;
  • Transport of intensity;
  • Computational imaging;
  • Biomedical, clinical, and medical applications of QPM;
  • Optical diffraction tomography;
  • Deep learning and neural networks related to QPM and applications;
  • Digital staining;
  • Optical information acquisition.

Dr. Ying Ma
Dr. Shaohui Zhang
Dr. Sha An
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Photonics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • quantitative phase imaging
  • quantitative phase microscopy
  • phase retrieval
  • label free
  • ptychography
  • 3D Imaging
  • 3D tomography

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

13 pages, 3166 KiB  
Article
Dynamic Measurement of Flowing Microparticles in Microfluidics Using Pulsed Modulated Digital Holographic Microscopy
by Yunze Lei, Yuge Li, Xiaofang Wang, Kequn Zhuo, Ying Ma, Sha An, Juanjuan Zheng, Kai Wen, Lihe Yan and Peng Gao
Photonics 2025, 12(5), 411; https://doi.org/10.3390/photonics12050411 - 24 Apr 2025
Viewed by 132
Abstract
We propose a pulsed modulated digital holographic microscopy (PM-DHM) technique for the dynamic measurement of flowing microparticles in microfluidic systems. By digitally tuning the pulse width and the repetition rate of a laser source within a single-frame exposure, this method enables the recording [...] Read more.
We propose a pulsed modulated digital holographic microscopy (PM-DHM) technique for the dynamic measurement of flowing microparticles in microfluidic systems. By digitally tuning the pulse width and the repetition rate of a laser source within a single-frame exposure, this method enables the recording of multiple images of flowing microparticles at different time points within a single hologram, allowing the quantification of velocity and acceleration. We demonstrate the feasibility of PM-DHM by measuring the velocity, acceleration, and forces exerted on PMMA microspheres and red blood cells flowing in microfluidic chips. Compared to traditional frame-sampling-based imaging methods, this technique has a much higher time resolution (in a range of microseconds) that is limited only by the pulse duration. This method demonstrates significant potential for high-throughput label-free flow cytometry detection and offers promising applications in drug development and cell analysis. Full article
(This article belongs to the Special Issue Advanced Quantitative Phase Microscopy: Techniques and Applications)
Show Figures

Figure 1

12 pages, 3924 KiB  
Article
A Denoise Network for Structured Illumination Microscopy with Low-Light Exposure
by Xin Liu, Jinze Li, Liangfeng Song, Kequn Zhuo, Kai Wen, Sha An, Ying Ma, Juanjuan Zheng and Peng Gao
Photonics 2024, 11(8), 776; https://doi.org/10.3390/photonics11080776 - 21 Aug 2024
Viewed by 1316
Abstract
Super-resolution structured illumination microscopy (SR-SIM) is one of the important techniques that are most suitable for live-cell imaging. The reconstructed SR-SIM images are noisy once the raw images are recorded with low-light exposure. Here, we propose a new network (entitled the ND-SIM network) [...] Read more.
Super-resolution structured illumination microscopy (SR-SIM) is one of the important techniques that are most suitable for live-cell imaging. The reconstructed SR-SIM images are noisy once the raw images are recorded with low-light exposure. Here, we propose a new network (entitled the ND-SIM network) to denoise the SR images reconstructed using frequency-domain algorithms (FDAs). We demonstrate that ND-SIM can yield artifact-free SR images using raw images with an average photon count down to 20 per pixel while achieving comparable resolution to the ground truth (GT) obtained with high-light exposure. We can envisage that the ND-SIM will be widely applied for the long-term, super-resolution live-cell imaging of various bioprocesses in the future. Full article
(This article belongs to the Special Issue Advanced Quantitative Phase Microscopy: Techniques and Applications)
Show Figures

Figure 1

Back to TopTop