Recent Advances in Multiphoton Microscopy

A special issue of Photonics (ISSN 2304-6732).

Deadline for manuscript submissions: closed (10 December 2023) | Viewed by 4286

Special Issue Editors


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Guest Editor
Department of Neuroscience and Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, USA
Interests: strong-field optics; high-field science; ultrafast lasers; multi-photon microscopy

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Guest Editor
Neuroscience Department, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA
Interests: developing cutting edge multiphoton microscopes; examining brain complexity via the lenses of engineering, optics, photonics, behavioral, and computational neuroscience

Special Issue Information

Dear Colleagues,

Multiphoton microscopy enables sub-micron-scale spatial resolution of structures deep within scattering media that are inaccessbile via other imaging modalities. Additionally, high-speed scanners, detectors, and indicators provide sufficient temporal resolution to capture the dynamics of biological systems in vivo, such as the brain. Researchers and scientists around the world continue to develop multiphoton microscope technology that enables the interrogation of wider fields of view, larger depths, and/or faster dynamics for various applications. Whether through the direct manipulation of laser light, advancements in detector technology, or signal processing, the field of multiphoton microscopy is advancing rapidly.

This multi-disciplinary pursuit involves a broad range of scientists from technology innovators to researchers working in very niche fields. This Special Issue invites researchers to cross-pollinate the broader field of multiphoton microscopy with their technological advancements. Topics of interest include but are not limited to:

  • Three-photon microscopy;
  • Third-harmonic generation microscopy;
  • Wide-field multi-photon microscopy;
  • High-speed/high-throughput imaging;
  • Contrast enhancement in multiphoton microscopy;
  • New techniques or implementations of light manipulation;
  • Hardware miniaturization;
  • Advances in image processing or detector technology.

Dr. Deano M. Farinella
Dr. Murat Yildirim
Guest Editors

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Keywords

  • multiphoton microscopy
  • three-photon microscopy
  • wide-field microscopy
  • SHG
  • THG

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Published Papers (1 paper)

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Review

23 pages, 6863 KiB  
Review
Advances in Ultrafast Fiber Lasers for Multiphoton Microscopy in Neuroscience
by Thulasi Srinivasan and Murat Yildirim
Photonics 2023, 10(12), 1307; https://doi.org/10.3390/photonics10121307 - 26 Nov 2023
Cited by 5 | Viewed by 3632
Abstract
Multiphoton microscopy (MPM) has emerged as a vital tool in neuroscience, enabling deeper imaging with a broader field of view, as well as faster and sub-cellular resolution. Recent innovations in ultrafast fiber laser technology have revolutionized MPM applications in living brains, offering advantages [...] Read more.
Multiphoton microscopy (MPM) has emerged as a vital tool in neuroscience, enabling deeper imaging with a broader field of view, as well as faster and sub-cellular resolution. Recent innovations in ultrafast fiber laser technology have revolutionized MPM applications in living brains, offering advantages like cost-effectiveness and user-friendliness. In this review, we explore the progress in ultrafast fiber laser technology, focusing on its integration into MPM for neuroscience research. We also examine the utility of femtosecond fiber lasers in fluorescence and label-free two- and three-photon microscopy applications within the field. Furthermore, we delve into future possibilities, including next-generation fiber laser designs, novel laser characteristics, and their potential for achieving high spatial and temporal resolution imaging. We also discuss the integration of fiber lasers with implanted microscopes, opening doors for clinical and fundamental neuroscience investigations. Full article
(This article belongs to the Special Issue Recent Advances in Multiphoton Microscopy)
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