Advances in Drug Delivery Systems for Leishmaniasis Therapy

A special issue of Pharmaceutics (ISSN 1999-4923). This special issue belongs to the section "Drug Targeting and Design".

Deadline for manuscript submissions: closed (31 January 2026) | Viewed by 1210

Special Issue Editors


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Guest Editor
Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 580, Bloco 13, São Paulo 05508-000, SP, Brazil
Interests: dendrimers; pharmacy; medicinal chemistry; drug design of candidates for malaria; Chagas disease; leishmaniasis; tuberculosis; drug delivery; nanomaterial

E-Mail Website
Guest Editor
Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo (USP), Professor Lineu Prestes Avenue, 580, Building 13, São Paulo 05508-900, SP, Brazil
Interests: dendrimers; molecular modification; latency; molecular modeling; chemical and enzymatic stability studies of dendrimeric prodrugs

E-Mail Website
Guest Editor
Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo (USP), Professor Lineu Prestes Avenue, 580, Building 13, São Paulo 05508-900, SP, Brazil
Interests: polymers; prodrugs; dendrimers; drug design and synthesis; neglected diseases

Special Issue Information

Dear Colleagues,

We are pleased to invite you to submit a contribution to this Special Issue “Advances in Drug Delivery Systems for Leishmaniasis Therapy”.

This Special Issue concentrates on the research of advanced systems that are potentially useful for the delivery of drugs or bioactive compounds in the therapy of leishmaniasis, one of the most relevant neglected diseases, which affect many impoverished regions on the planet. Reviews and original research involving new trends and materials used in drug delivery, including smart polymers, dendrimers, nanotubes, and stimuli-responsive or self-immolative systems, among others, as different nanoparticles are strongly expected.

Your contributions are welcome, and we look forward to receiving your interesting manuscripts.

Prof. Dr. Elizabeth Igne Ferreira
Prof. Dr. Jeanine Giarolla
Prof. Dr. Soraya Da Silva Santos
Guest Editors

Manuscript Submission Information

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Keywords

  • delivery systems
  • leishmanicides
  • bioactive compounds in Leishmania sp.
  • advanced systems for drug delivery
  • oral and topical administration

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Published Papers (1 paper)

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Research

26 pages, 3759 KB  
Article
Development of DNA Aptamers Against Leishmania infantum GP63 Protein for Therapeutic and Diagnostic Applications
by Lucía Román-Álamo, Daniela Currea-Ayala, Gabriel S. Oliveira, Antonino Nicolò Fallica, Timen Mooren, Yunuen Avalos-Padilla and Xavier Fernàndez-Busquets
Pharmaceutics 2026, 18(3), 304; https://doi.org/10.3390/pharmaceutics18030304 - 28 Feb 2026
Viewed by 785
Abstract
Background/Objectives: Leishmaniasis is a disease affecting millions of people caused by parasites of the genus Leishmania. The GP63 protein of Leishmania infantum (LiGP63) is one of its major surface antigens and a main virulence factor, playing a role in the [...] Read more.
Background/Objectives: Leishmaniasis is a disease affecting millions of people caused by parasites of the genus Leishmania. The GP63 protein of Leishmania infantum (LiGP63) is one of its major surface antigens and a main virulence factor, playing a role in the adhesion of extracellular promastigote stages to macrophages and in the survival of intracellular amastigotes. Methods: Here, DNA aptamers have been developed against LiGP63 through the systematic evolution of ligands by exponential enrichment. Results: Twenty individual aptamer sequences were characterized using confocal fluorescence microscopy and flow cytometry analysis, and 14 of them had targeting to more than 70% of L. infantum promastigotes with different subcellular localization patterns. Subsequent dot blot analyses narrowed down the selection to five candidates for further characterization through an aptamer-linked immobilized sorbent assay where it was possible to detect endogenous LiGP63 in L. infantum promastigote lysates. The five selected aptamers recognized the recombinant LiGP63 protein with binding affinities ranging from 0.3 to 2.1 µM. Promastigotes preincubated with LiGP63Apt-4, -27 and -28 exhibited a significantly reduced adhesion to and infection of RAW 264.7 macrophages. Moreover, when LiGP63Apt-4 and -28 were conjugated to liposomes, these two aptamers significantly enhanced the targeting to L. infantum promastigotes compared to plain liposomes. Conclusions: Given their improved stability and cost-effectiveness over antibodies, the aptamers evolved here represent promising candidates for new therapeutic and diagnostic approaches and for future nanoparticle-based drug delivery strategies in leishmaniasis. Full article
(This article belongs to the Special Issue Advances in Drug Delivery Systems for Leishmaniasis Therapy)
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