Advances in Molecular Characterization and Epidemiology of Plant Viruses

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Viral Pathogens".

Deadline for manuscript submissions: 31 January 2026 | Viewed by 269

Special Issue Editor


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Guest Editor
Unit of Phytopathology and Agricultural Modelling, CONICET-INTA, Cordoba 5119, Argentina
Interests: virus; taxonomy; metatranscriptomics; data mining
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue aims to explore the latest breakthroughs in understanding plant viral diseases at both the molecular and epidemiological levels. As global agricultural systems face increasing challenges from viral infections, this Special Issue sets out to highlight cutting-edge research in virus detection, molecular diagnostics, and the dynamics of virus spread in crops. We aim to delve into novel techniques for characterizing plant viruses, understanding their interactions with hosts, and assessing the impact on crop yield and food security. This Special Issue aims to provide valuable insights into how to improve plant health management strategies worldwide.

All types of articles are welcome to be submitted, including short reports, primary research articles, and reviews. We look forward to receiving your contributions.

Dr. Nicolas Bejerman
Guest Editor

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Keywords

  • plant viruses
  • metatranscriptomics
  • epidemiology
  • viral diseases
  • virus detection

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Published Papers (1 paper)

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Research

18 pages, 2569 KB  
Article
Characterization of the Pepper Virome in Oklahoma Reveals Emerging RNA and DNA Viruses
by Caleb Paslay and Akhtar Ali
Pathogens 2025, 14(10), 1035; https://doi.org/10.3390/pathogens14101035 - 13 Oct 2025
Abstract
Pepper (Capsicum spp.) is an economically valuable crop worldwide including in the United States due to its nutritional benefits in human health and widespread use as a spice or vegetable. Although numerous viruses have been reported infecting peppers in the USA, little [...] Read more.
Pepper (Capsicum spp.) is an economically valuable crop worldwide including in the United States due to its nutritional benefits in human health and widespread use as a spice or vegetable. Although numerous viruses have been reported infecting peppers in the USA, little is known about the diversity and distribution of pepper-infecting viruses in Oklahoma. To address this knowledge gap, we conducted a comprehensive pepper virome study to identify viruses infecting pepper and their incidence across six different counties in Oklahoma. A total of 310 plant samples including pepper and other potential hosts were collected during the 2021 and 2022 growing seasons. Samples were analyzed using high-throughput sequencing (HTS) and/or reverse transcription-polymerase chain reaction (RT-PCR) assays. Viral contigs identified via HTS were further validated through RT-PCR or PCR assays followed by Sanger sequencing. In total, 17 distinct viruses were detected, including 15 RNA and two DNA viruses, with several representing putatively novel findings. The most prevalent virus was beet curly top virus (BCTV), followed by tomato yellow leaf curl virus (TYLCV), potato yellow dwarf virus/constricta yellow dwarf virus (PYDV/CYDV), and pepper mild mottle virus (PMMoV). Virus incidence varied by season and location, with some surveys showing infection rates exceeding 80%. This study provides the first in-depth characterization of the pepper virome in Oklahoma and valuable insights into the prevalence and distribution of pepper-infecting viruses. These findings will support the development of informed, targeted strategies for virus detection and management in pepper production systems. Full article
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