Special Issue "Applications of Cytometry in Translational Medicine: From Bench to the Clinic"

A special issue of Methods and Protocols (ISSN 2409-9279). This special issue belongs to the section "Biomedical Sciences and Physiology".

Deadline for manuscript submissions: 31 July 2021.

Special Issue Editors

Dr. Georgios S. Markopoulos
E-Mail
Guest Editor
Laboratory of Biology, School of Medicine, Faculty of Health Sciences, University of Ioannina, University Campus, 45110 Ioannina, Greece
Interests: Cancer Research; Translational Oncology; Non-Coding Genome; Flow Cytometry
Dr. George Vartholomatos
E-Mail
Guest Editor
Unit of Molecular biology, Laboratory of Haematology, University Hospital of Ioannina, Ioannina, Greece
Interests: Intraoperative Flow Cytometry, Translational Oncology, Flow Cytometry
Dr. George Alexiou
E-Mail Website
Guest Editor
Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece
Interests: neurosurgery; pediatric neurosurgery; spine surgery; molecular biology; nuclear medicine; neuroradiology; flow cytometry

Special Issue Information

Dear Colleagues,

Cytometry has emerged as an independent research field with numerous applications in medicine. During the last years, many methodological and technological advances in the field have rendered cytometry an invaluable tool in biomedical research and diagnosis. From molecular oncology to Mendelian and infectious disease, cytometry is the method of choice for the quantification of cellular phenotypes. 

The aim of the current Special Issue, “Applications of Cytometry in Translational Medicine: From Bench to the Clinic”, is to provide a comprehensive collection of cytometry methods used to study human disease. We welcome, primary or review articles covering (not exclusively) the following topics:

  • Novel methods for phenotypic analysis of human cell populations
  • Technological and methodological advantages of diagnostic techniques (i.e., multiparametric analysis in hematology, oncology, immunology, etc.)
  • Novel diagnostic methods
  • Reviews describing the state of the art in the field
  • Contribution of cytometry to translational medicine

The aim of the current Special Issue is to serve as a forum for researchers applying cytometry in translational research. We hope that knowledge dissemination will provide valuable research tools to fight human diseases.

Dr. Georgios S. Markopoulos
Dr. George Vartholomatos
Dr. George Alexiou
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Methods and Protocols is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

Jump to: Review

Open AccessArticle
Assessment of Protocol Impact on Subjectivity Uncertainty When Analyzing Peripheral Blood Mononuclear Cell Flow Cytometry Data Files
Methods Protoc. 2021, 4(2), 24; https://doi.org/10.3390/mps4020024 - 30 Mar 2021
Viewed by 273
Abstract
Measured variability of product within Cell and Gene Therapy (CGT) manufacturing arises from numerous sources across pre-analytical to post-analytical phases of testing. Operators are a function of the manufacturing process and are an important source of variability as a result of personal differences [...] Read more.
Measured variability of product within Cell and Gene Therapy (CGT) manufacturing arises from numerous sources across pre-analytical to post-analytical phases of testing. Operators are a function of the manufacturing process and are an important source of variability as a result of personal differences impacted by numerous factors. This research uses measurement uncertainty in comparison to Coefficient of Variation to quantify variation of participants when they complete Flow Cytometry data analysis through a 5-step gating sequence. Two study stages captured participants applying gates using their own judgement, and then following a diagrammatical protocol, respectively. Measurement uncertainty was quantified for each participant (and analysis phase) by following Guide to the Expression of Uncertainty in Measurement protocols, combining their standard deviations in quadrature from each gating step in the respective protocols. When participants followed a diagrammatical protocol, variation between participants reduced by 57%, increasing confidence in a more uniform reported cell count percentage. Measurement uncertainty provided greater resolution to the analysis processes, identifying that most variability contributed in the Flow Cytometry gating process is from the very first gate, where isolating target cells from dead or dying cells is required. This work has demonstrated the potential for greater usage of measurement uncertainty within CGT manufacturing scenarios, due to the resolution it provides for root cause analysis and continuous improvement. Full article
Show Figures

Figure 1

Review

Jump to: Research

Open AccessReview
The Past, Present and Future of Flow Cytometry in Central Nervous System Malignancies
Methods Protoc. 2021, 4(1), 11; https://doi.org/10.3390/mps4010011 - 26 Jan 2021
Viewed by 535
Abstract
Central nervous system malignancies (CNSMs) are categorized among the most aggressive and deadly types of cancer. The low median survival in patients with CNSMs is partly explained by the objective difficulties of brain surgeries as well as by the acquired chemoresistance of CNSM [...] Read more.
Central nervous system malignancies (CNSMs) are categorized among the most aggressive and deadly types of cancer. The low median survival in patients with CNSMs is partly explained by the objective difficulties of brain surgeries as well as by the acquired chemoresistance of CNSM cells. Flow Cytometry is an analytical technique with the ability to quantify cell phenotype and to categorize cell populations on the basis of their characteristics. In the current review, we summarize the Flow Cytometry methodologies that have been used to study different phenotypic aspects of CNSMs. These include DNA content analysis for the determination of malignancy status and phenotypic characterization, as well as the methodologies used during the development of novel therapeutic agents. We conclude with the historical and current utility of Flow Cytometry in the field, and we propose how we can exploit current and possible future methodologies in the battle against this dreadful type of malignancy. Full article
Show Figures

Figure 1

Back to TopTop