Detection Techniques and Assay Development for Bacterial Susceptibility and Resistance

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Antimicrobial Agents and Resistance".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 1742

Special Issue Editor


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Guest Editor
Department of Electrical and Computer Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
Interests: bacterial susceptibility; bacterial resistance; materials
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In recent years, there has been growing interest in the development of rapid, high-sensitivity, and high throughput methodologies for applications in antimicrobial treatments, analysis and assessment of microbial growth and proliferation. This Special Issue of Microorganisms focuses on advances in detection techniques and assay development for monitoring susceptibility and resistance of bacterial strains to antimicrobial treatments. Topics include but are not limited to sensor technologies, emerging chemical/biochemical assays, and imaging techniques.

You may choose our Joint Special Issue in LabMed.

Dr. Pavel Ivanoff Reyes
Guest Editor

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Keywords

  • antimicrobial treatments
  • chemical assays
  • susceptibility
  • resistance

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Published Papers (1 paper)

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Research

13 pages, 1633 KB  
Article
Resistance Trends in Klebsiella pneumoniae Strains Isolated from Bloodstream Infections in a Tertiary Care Hospital over a Period of 7 Years
by Alina Maria Borcan, Elena Rotaru, Georgiana Radu, Elena Liliana Costea, Calin Andrei Borcan, Mihaela-Cristina Olariu and Madalina Simoiu
Microorganisms 2025, 13(11), 2451; https://doi.org/10.3390/microorganisms13112451 - 25 Oct 2025
Cited by 4 | Viewed by 1436
Abstract
Klebsiella pneumoniae is one of the top pathogens causing bloodstream infections (BSIs) worldwide. The rise of carbapenem-resistant K. pneumoniae (CRKP) and multidrug-resistant (MDR) strains is of particular concern as therapeutic options are limited. Analyzing local resistance profiles is essential for the success of [...] Read more.
Klebsiella pneumoniae is one of the top pathogens causing bloodstream infections (BSIs) worldwide. The rise of carbapenem-resistant K. pneumoniae (CRKP) and multidrug-resistant (MDR) strains is of particular concern as therapeutic options are limited. Analyzing local resistance profiles is essential for the success of antibiotic stewardship strategies. This study aims to explore the resistance profiles of K. pneumoniae strains identified in BSI in a tertiary care hospital over 7 years. Automated systems were used to test antibiotic susceptibility. Results were interpreted according to EUCAST clinical breakpoints. The rate of multidrug resistance (MDR) was 57.6%. The percentage of ESBL producers was 54.5%, and the percentage of carbapenemase producers was 43.2%. Overall resistance rates to other antibiotics were 47.1% to ciprofloxacin, 31.4% to gentamicin, 25.7% to amikacin, 20.9% to colistin, 19.6% to Fosfomycin, and 44.5% to trimethoprim/sulfamethoxazole. The highest resistance to colistin was recorded in 2023 (28%). More than half of the strains in the study were MDR and ESBL producers. K. pneumoniae resistance to colistin has increased during the last 7 years. The rates of carbapenemase-producing bacteria (CPB) are on the rise. The most frequently co-harboring carbapenemases were NDM and OXA-48. Local antibiotic resistance rates are crucial in implementing an effective antibiotic stewardship strategy. Full article
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