Overview of Foodborne Pathogens and Antimicrobial Resistance

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (30 November 2024) | Viewed by 6905

Special Issue Editor


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Guest Editor
Centers for Disease Control and Prevention, Atlanta, GA, USA
Interests: foodborne; gene

Special Issue Information

Dear Colleagues,

The detection, identification and characterization of foodborne pathogens are critical for assessing pathogenicity and eliminating future illnesses. In this Special Issue, we highlight the involvement of pathogens, Campylobacter spp., Cronobacter ssp., Listeria spp., non-typhoidal Salmonella, Shiga-toxin-producing Escherichia coli (STEC) and others, in illnesses. The objective of this Special Issue is to present articles that study the occurrence of these pathogens in foods in developing and developed economies, as well as the risks they pose to consumers. For this Special Issue, we welcome articles focused on the detection (conventional and molecular) of these foodborne pathogens, with an emphasis on the application of these methods and downstream characterization (antimicrobial resistance determinants, toxin, virulence determinants, cgMLST, etc.). The identification and characterization of these organisms are important for defining outbreaks and/or determining what may contribute to an organism’s pathogenicity and virulence. We are also interested in One Health studies, as well as those related to surveillance or outbreaks involving these organisms.

Dr. Rebecca Lynn Lindsey
Guest Editor

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Keywords

  • foodborne pathogens
  • virulence
  • antimicrobial resistance
  • Campylobacter spp.
  • Cronobacter spp.
  • non-typhoidal Salmonella
  • shiga-toxin-producing Escherichia coli (STEC)
  • other Escherichia
  • Listeria spp.
  • public health
  • food safety
  • One Health
  • surveillance

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Published Papers (3 papers)

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13 pages, 1528 KiB  
Article
Impact of Various Washing Protocols on the Mitigation of Escherichia coli Contamination in Raw Salad Vegetables
by Fahad M. Alreshoodi, Bassam Alsuliman, Norah M. Alotaibi, Afnan Althobaiti, Lenah E. Mukhtar, Sarah Alsaleh, Abdullah A. Alajlan, Saleh I. Alakeel, Fahad M. Alshabrmi, Tarique Sarwar and Sulaiman M. Alajel
Microorganisms 2024, 12(10), 2103; https://doi.org/10.3390/microorganisms12102103 - 21 Oct 2024
Viewed by 2675
Abstract
Vegetables are an essential component of a balanced diet. The consumption of ready-to-eat foods may lead to the risk of infections and illnesses due to microbial contamination. To mitigate the potential of microbial contamination risks, it is critical to promote safe handling practices [...] Read more.
Vegetables are an essential component of a balanced diet. The consumption of ready-to-eat foods may lead to the risk of infections and illnesses due to microbial contamination. To mitigate the potential of microbial contamination risks, it is critical to promote safe handling practices among consumers. In this study, our research evaluated the efficacy of different vegetable washing methods, specifically with lettuce, tomato, and cucumber, to establish optimal practices for reducing microbial contamination. This study consisted of two phases. Initially, a survey was distributed to 150 volunteers using snowball sampling to assess everyday vegetable handling and washing methods. The survey’s results identified four predominant methods: washing with a 5% vinegar solution for 3 min followed by tap water rinse (37.3% of participants), rinsing with tap water for 1 min (29.3%), washing with a 5% salt solution (vegetable soap) for 3 min followed by a tap water rinse (16.6%), and a 3 min tap water rinse (14%). A minor segment (3.33%) reported not washing their vegetables at all. The survey’s findings guided the second phase, which tested the aforementioned washing protocols’ effectiveness in reducing Escherichia coli (E. coli) levels on spiked contaminated salad vegetables. The tested vegetables were sterilized using UV light, inoculated with 0.5 McFarland E. coli, and then washed using the four identified methods. After that, E. coli enumeration after washing was performed using 3M™ Petrifilm and the comparison was analyzed via one-way ANOVA. During this study, it was revealed that the cucumbers had the highest E. coli contamination levels in comparison to the lettuce and tomato after washing. Interestingly, by comparing the three washing methods, it was found that washing the vegetables with vinegar proved to be the most effective solution for reducing microbial presence on both lettuce and cucumbers. Notably, the natural smoothness of tomato skin led to no significant differences in contamination levels across washing methods. In summary, vinegar washing effectively reduces microbial contamination from salad vegetables, highlighting the need for informed consumer practices to prevent foodborne outbreaks. This study emphasizes the importance of monitoring contamination sources and using safe washing techniques. Full article
(This article belongs to the Special Issue Overview of Foodborne Pathogens and Antimicrobial Resistance)
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25 pages, 4546 KiB  
Article
Genetic Diversity in Salmonella enterica in Outbreaks of Foodborne and Zoonotic Origin in the USA in 2006–2017
by Eija Trees, Heather A. Carleton, Jason P. Folster, Laura Gieraltowski, Kelley Hise, Molly Leeper, Thai-An Nguyen, Angela Poates, Ashley Sabol, Kaitlin A. Tagg, Beth Tolar, Michael Vasser, Hattie E. Webb, Matthew Wise and Rebecca L. Lindsey
Microorganisms 2024, 12(8), 1563; https://doi.org/10.3390/microorganisms12081563 - 31 Jul 2024
Cited by 3 | Viewed by 2045
Abstract
Whole genome sequencing is replacing traditional laboratory surveillance methods as the primary tool to track and characterize clusters and outbreaks of the foodborne and zoonotic pathogen Salmonella enterica (S. enterica). In this study, 438 S. enterica isolates representing 35 serovars and [...] Read more.
Whole genome sequencing is replacing traditional laboratory surveillance methods as the primary tool to track and characterize clusters and outbreaks of the foodborne and zoonotic pathogen Salmonella enterica (S. enterica). In this study, 438 S. enterica isolates representing 35 serovars and 13 broad vehicle categories from one hundred epidemiologically confirmed outbreaks were evaluated for genetic variation to develop epidemiologically relevant interpretation guidelines for Salmonella disease cluster detection. The Illumina sequences were analyzed by core genome multi-locus sequence typing (cgMLST) and screened for antimicrobial resistance (AR) determinants and plasmids. Ninety-three of the one hundred outbreaks exhibited a close allele range (less than 10 allele differences with a subset closer than 5). The remaining seven outbreaks showed increased variation, of which three were considered polyclonal. A total of 16 and 28 outbreaks, respectively, showed variations in the AR and plasmid profiles. The serovars Newport and I 4,[5],12:i:-, as well as the zoonotic and poultry product vehicles, were overrepresented among the outbreaks, showing increased variation. A close allele range in cgMLST profiles can be considered a reliable proxy for epidemiological relatedness for the vast majority of S. enterica outbreak investigations. Variations associated with mobile elements happen relatively frequently during outbreaks and could be reflective of changing selective pressures. Full article
(This article belongs to the Special Issue Overview of Foodborne Pathogens and Antimicrobial Resistance)
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8 pages, 435 KiB  
Brief Report
Prevalence and Characterization of Salmonella during Pork Sausage Manufacturing
by Lauren R. Wottlin, Roger B. Harvey, Keri N. Norman, Robert E. Droleskey, Kathleen Andrews, Steve J. Jackson, Robin C. Anderson and Toni L. Poole
Microorganisms 2024, 12(8), 1599; https://doi.org/10.3390/microorganisms12081599 - 6 Aug 2024
Cited by 2 | Viewed by 1527
Abstract
Pork carcasses and meat may harbor Salmonella and may contaminate other products during harvest and fabrication. Sources of contamination include manure on hides, environmental contamination, ingredients from external sources, and lymph nodes. Swine lymph nodes are often incorporated into ground meat, as their [...] Read more.
Pork carcasses and meat may harbor Salmonella and may contaminate other products during harvest and fabrication. Sources of contamination include manure on hides, environmental contamination, ingredients from external sources, and lymph nodes. Swine lymph nodes are often incorporated into ground meat, as their anatomical location makes removal labor prohibitive. A sausage processing plant in the midwestern United States was sampled monthly (except for December) from May 2021 to April 2022 to enumerate Salmonella and Enterobacteriaceae (EB) throughout the sausage manufacturing process to determine high-risk stages and efficiency of existing in-plant interventions. Salmonella serotypes and antimicrobial susceptibility were evaluated on a subset of isolates recovered at the end phases of sausage production. In each collection, samples were taken from the carcasses of eight sows through 11 stages of sausage manufacturing. A total of 830 samples were cultured. Thirty-four Salmonella were isolated from the final three production stages; of these, there were eleven serotypes. Three isolates displayed resistance to ampicillin, whereas the remainder of the isolates were pan-susceptible to the antimicrobials tested. Salmonella and EB were significantly reduced (p < 0.001) by acid washes at different stages of production, and the results point to the beneficial effects of interventions to lessen Salmonella concentrations in retail products. Full article
(This article belongs to the Special Issue Overview of Foodborne Pathogens and Antimicrobial Resistance)
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