Advances in Microparasite Research

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Molecular Microbiology and Immunology".

Deadline for manuscript submissions: 31 January 2026 | Viewed by 691

Special Issue Editors


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Guest Editor
CIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal
Interests: microtubule cytoskeleton; Apicomplexa protozoa; host-parasite interaction
Special Issues, Collections and Topics in MDPI journals

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Guest Editor Assistant
I-MVET (Research in Veterinary Medicine), Faculty of Veterinary Medicine, Lusófona University-Lisbon University Centre, Lisbon, Portugal
Interests: Apicomplexa; vector-borne pathogens; molecular cell biology; parasitic diseases; One Health

Special Issue Information

Dear Colleagues,

Recent years have brought remarkable progress and emerging trends, shaping the field of parasitology. Breakthroughs in molecular biology, genomics, and transcriptomics have enabled unprecedented insights into parasite biology, host-parasite interactions, and disease mechanisms. Advances in high-throughput sequencing and CRISPR-Cas9 gene editing have empowered researchers to identify essential parasite genes, unravel complex life cycles, and pinpoint novel drug and vaccine targets, particularly in major pathogens such as Plasmodium falciparum, and Toxoplasma gondii.

The Special Issue invites submissions that showcase cutting-edge developments and innovative approaches across the diverse landscape of microparasite research, with a particular focus on protozoan and chromistan parasites. This Special Issue will focus on original research, reviews, and methodological advances that deepen our understanding of microparasite biology, host-parasite interactions, and disease mechanisms. We are particularly interested in studies leveraging molecular biology, genomics, transcriptomics, and proteomics to unravel the complexities of parasite life cycles, pathogenesis, and adaptation. Contributions that employ novel technologies—such as high-throughput sequencing, CRISPR-Cas9 gene editing, or artificial intelligence in diagnostics—are highly encouraged, as are papers presenting new diagnostic tools, drug and vaccine targets, and innovative therapeutic strategies. The Special Issue also seeks work addressing the epidemiology and control of parasitic diseases, especially in the context of emerging challenges such as climate change, urbanization, and drug resistance. Interdisciplinary studies that integrate ecological, clinical, and computational perspectives are welcome, as are submissions focused on neglected tropical diseases and one health implications.

By bringing together a diverse range of topics and methodologies, this Special Issue aims to provide a comprehensive overview of the latest advances in the study of microparasites and to foster collaboration and knowledge exchange within the scientific community. Authors are encouraged to submit manuscripts that present significant scientific contributions and novel insights, helping to shape the future direction of parasitology research.

Dr. Sofia Nolasco
Guest Editor

Dr. Inês L. S. Delgado
Guest Editor Assistant

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microparasites
  • molecular parasitology
  • parasitic disease diagnostics
  • parasitic disease epidemiology
  • host–parasite interactions
  • parasite genomics/transcriptomics
  • drug/vaccine targets
  • drug resistance
  • neglected tropical diseases
  • One Health approach

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Published Papers (1 paper)

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Research

25 pages, 3380 KB  
Article
Vaccination with a Trypanosoma cruzi Protein Extract Plus BCG∆BCG1419c Promotes a Balanced Th1/Th2 Immune Profile That Improves Control of Acute Chagas Disease in BALB/c Mice
by Olivia Rodríguez-Morales, Minerva Arce-Fonseca, Dulce Mata-Espinosa, Alberto Aranda-Fraustro, José Luis Rosales-Encina and Mario Alberto Flores-Valdez
Microorganisms 2025, 13(11), 2447; https://doi.org/10.3390/microorganisms13112447 - 25 Oct 2025
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Abstract
Adjuvants in vaccine candidates against Chagas disease (ChD) have been tested with the aim of improving efficacy against this disease. Trypanosoma cruzi total protein extract (TcTPE) combined with Mycobacterium bovis BCG Pasteur strain ATCC 35734 or its isogenic derivative mutant BCGΔBCG1419c—in which the [...] Read more.
Adjuvants in vaccine candidates against Chagas disease (ChD) have been tested with the aim of improving efficacy against this disease. Trypanosoma cruzi total protein extract (TcTPE) combined with Mycobacterium bovis BCG Pasteur strain ATCC 35734 or its isogenic derivative mutant BCGΔBCG1419c—in which the BCG1419c gene was deleted—were used as a vaccine formulation in BALB/c mice. After immunization and T. cruzi challenge, parasitological and clinical parameters of acute stage were recorded. Antibody titers, cytokine concentrations, macroscopic abnormalities, and histological analysis of experimental ChD were determined. The vaccine formulation with the combination of TcTPE and BCGΔBCG1419c, used as the adjuvant, reduced parasitemia by 50%, promoted a survival rate of 80%, improved the clinical status, favored greater body weight gain, induced high titers of specific anti-T. cruzi IgG antibodies and some cytokines, leading to a balanced Th1/Th2 immunological profile as well as a lower degree of inflammation and tissue damage (50% reduction). A good protective effect in the acute stage of experimental ChD was observed by favorably modulating the immune response and reducing heart and muscle damage, therefore, highlighting that the combination tested here for the control of ChD provides a promising basis that warrants further investigation to validate a future potential translation to humans. Full article
(This article belongs to the Special Issue Advances in Microparasite Research)
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