Microbial Fermentation, Food and Food Sustainability, 2nd Edition

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: 31 October 2026 | Viewed by 1703

Editors


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Guest Editor
Institute of Sciences of Food Production (CNR-ISPA), National Research Council of Italy, 70126 Bari, Italy
Interests: LAB; probiotics; biotechnology; food product innovation; waste valorization
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Special Issue Information

Dear Colleagues,

This Special Issue is the continuation of our previous Special Issue, entitled “Microbial Fermentation, Food and Food Sustainability”.

Biotechnologies have been widely used in food production for thousands of years. Nevertheless, new approaches have recently been proposed to meet the market demand for high-quality and sustainable products.

Innovative fermentation bioprocesses can include alternative ingredients, by-products, and waste derived from the agrifood sector, and often start with the characterization of the associated matrices and their microbiota. The fermentation performance and enzymatic activity of potential starters, the synthesis of functional compounds, the degradation of antinutritional factors, and the improvement of quality have played roles in the formulation of new food ingredients and products. Moreover, innovative non-food materials (e.g., feed, bioplastic, biofuels, and fertilizers) can be produced through the fermentation of food by-products.

The aim of this Special Issue is to compile original research articles and systematic reviews that focus on the valorization of alternative food matrices, by-products, and waste obtained through fermentation, as well as biotechnologies related to the production of traditional fermented ingredients and food products.

Topics of interest include, but are not limited to, the setup of new biotechnological processes, the characterization of the microbial community or starters involved in fermentation processes, and the characterization of the obtained products.

Dr. Marco Montemurro
Prof. Dr. Carlo Giuseppe Rizzello
Guest Editors

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Keywords

  • food
  • production
  • fermentation
  • microbial community
  • bioprocesses

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Published Papers (2 papers)

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Research

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21 pages, 6799 KB  
Article
Composite Microbial Solid-State Fermentation Enhances the Fermentation Quality, Nutritional Value, and Safety of Cottonseed Hulls: Insights Based on Physicochemical Detection and Untargeted Metabolomics
by Honghai Yang, Xiaoyan Zhou, Yuwei Ying, Yan Liu, Hanzuohere Yishake, Hongman Li and Caidie Wang
Microorganisms 2026, 14(7), 1456; https://doi.org/10.3390/microorganisms14071456 - 2 Jul 2026
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Abstract
Cottonseed hulls (CSH) are by-products of cotton processing, but their use in livestock feed is constrained by lignocellulose and free gossypol. In this study, solid-state fermentation of cottonseed hulls was optimized using Aspergillus niger, Aspergillus oryzae, and Lactobacillus plantarum through an [...] Read more.
Cottonseed hulls (CSH) are by-products of cotton processing, but their use in livestock feed is constrained by lignocellulose and free gossypol. In this study, solid-state fermentation of cottonseed hulls was optimized using Aspergillus niger, Aspergillus oryzae, and Lactobacillus plantarum through an orthogonal experimental design. Fermentation quality, nutrient composition, safety indicators, microstructure, and metabolic profiles were subsequently evaluated under the optimized conditions. The results showed that composite microbial fermentation significantly reduced pH and decreased NH3-N/TN by 42.35%, while increasing lactic acid content by 20.01 g/kg. Meanwhile, butyric acid was not detected. Compared with the non-inoculated control, neutral detergent fiber and acid detergent fiber were further degraded by 5.78% and 7.37%, respectively. In addition, free gossypol was reduced by 79.79% compared with untreated cottonseed hulls, and aflatoxin B1 content decreased by 60.11% compared with the non-inoculated control. Untargeted metabolomics revealed increased abundances of amino acids and bioactive small peptides, including L-arginine, Ile-Lys, Glu-Met, and L-isoleucyl-L-arginine. Taken together, these findings indicate that composite microbial fermentation may serve as an effective strategy for the detoxification and nutritional improvement of cottonseed hulls, providing a theoretical basis for the application of fermented cottonseed hulls as a feed resource. Full article
(This article belongs to the Special Issue Microbial Fermentation, Food and Food Sustainability, 2nd Edition)
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Review

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21 pages, 775 KB  
Review
Exploring the Diversity and Applications of Lactic Acid Bacteria from Tunisian Traditional Fermented Foods
by Sabrine Alebidi, Hana Mallek, Mariagiovanna Fragasso, Vittorio Capozzi, Ferid Abidi, Ines Essid, Giuseppe Spano and Hiba Selmi
Microorganisms 2026, 14(2), 383; https://doi.org/10.3390/microorganisms14020383 - 5 Feb 2026
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Abstract
Tunisian traditional fermented foods represent a valuable cultural heritage transmitted across generations and are highly appreciated by consumers for their distinctive flavours, textures, and nutraceutical value. This review provides the first comprehensive and exclusive overview of lactic acid bacteria (LAB) associated with Tunisian [...] Read more.
Tunisian traditional fermented foods represent a valuable cultural heritage transmitted across generations and are highly appreciated by consumers for their distinctive flavours, textures, and nutraceutical value. This review provides the first comprehensive and exclusive overview of lactic acid bacteria (LAB) associated with Tunisian traditional fermented foods, both plant- and animal-based. The overview integrates data across dairy, meat, fish, vegetable, and cereal matrices, highlighting the central role that LAB play in the processing of these foods, driving fermentation and shaping the quality and safety of final products. During fermentation, LAB produce a variety of bioactive metabolites, including organic acids, antimicrobial compounds, exopolysaccharides, enzymes, and vitamins, which enhance food safety, shelf life, nutritional quality, and health-promoting potential. The studies include evidence of LAB’s long history of safe use by humans, including the characterisation of autochthonous strains with protechnological, bioprotective, and probiotic properties, providing candidates for the design of starter, protective and probiotic cultures. By consolidating evidence on the relevance of microbial diversity, this review positions Tunisian LAB as valuable resources for both traditional food valorisation and innovative food system development. Importantly, key knowledge gaps are identified, including the limited application of omics-based tools, insufficient genomic safety assessments, and the lack of systematic analysis linking LAB diversity with the desired attributes to promote innovations. Overall, this review provides a structured framework for the valorisation of Tunisian agrofood heritage, bridging artisanal knowledge with modern food microbiology and offering strategic directions for future research, industrial translation, and sustainable innovation in fermented foods. Full article
(This article belongs to the Special Issue Microbial Fermentation, Food and Food Sustainability, 2nd Edition)
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