Topical Collection "Feature Papers Related to Metabolomic Profiling Technology"

Editor

Dr. Thusitha W. Rupasinghe
E-Mail Website
Collection Editor
School of BioSciences, The University of Melbourne, Parkville, VIC, Australia
Interests: lipidomics; metabolomics; bioanalytics; fluxomics; analytical chemistry; plant physiology
Special Issues, Collections and Topics in MDPI journals

Topical Collection Information

Dear Colleagues,

Metabolomics and lipidomics are emerging technologies which aim at the global characterization and quantification of small molecules and lipids within biological matrices including biofluids, cells, whole organs, and tissues. New emerging technology-based metabolite profiling has been used to track the change of levels of metabolites and lipids in biological systems due to many different situations. This Special Collection on metabolomic profiling technology aims to attract high-quality manuscripts in the metabolomics field. We encourage researchers from the metabolomics and lipidomics field to contribute manuscripts highlighting the latest development in their research field with new technology-based applications.

Dr. Thusitha Rupasinghe
Collection Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the collection website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Metabolomics
  • Lipidomic
  • High-resolution mass spectrometry
  • Untargeted and targeted
  • Data processing
  • Pathway mapping
  • Fluxomic

Published Papers (1 paper)

2021

Article
Benchmarking Non-Targeted Metabolomics Using Yeast-Derived Libraries
Metabolites 2021, 11(3), 160; https://doi.org/10.3390/metabo11030160 - 10 Mar 2021
Cited by 3 | Viewed by 1113
Abstract
Non-targeted analysis by high-resolution mass spectrometry (HRMS) is an essential discovery tool in metabolomics. To date, standardization and validation remain a challenge. Community-wide accepted cost-effective benchmark materials are lacking. In this work, we propose yeast (Pichia pastoris) extracts derived from fully [...] Read more.
Non-targeted analysis by high-resolution mass spectrometry (HRMS) is an essential discovery tool in metabolomics. To date, standardization and validation remain a challenge. Community-wide accepted cost-effective benchmark materials are lacking. In this work, we propose yeast (Pichia pastoris) extracts derived from fully controlled fermentations for this purpose. We established an open-source metabolite library of >200 identified metabolites based on compound identification by accurate mass, matching retention times, and MS/MS, as well as a comprehensive literature search. The library includes metabolites from the classes of (1) organic acids and derivatives (2) nucleosides, nucleotides, and analogs, (3) lipids and lipid-like molecules, (4) organic oxygen compounds, (5) organoheterocyclic compounds, (6) organic nitrogen compounds, and (7) benzoids at expected concentrations ranges of sub-nM to µM. As yeast is a eukaryotic organism, key regulatory elements are highly conserved between yeast and all annotated metabolites were also reported in the human metabolome database (HMDB). Orthogonal state-of-the-art reversed-phase (RP-) and hydrophilic interaction chromatography mass spectrometry (HILIC-MS) non-targeted analysis and authentic standards revealed that 104 out of the 206 confirmed metabolites were reproducibly recovered and stable over the course of three years when stored at −80 °C. Overall, 67 out of these 104 metabolites were identified with comparably stable areas over all three yeast fermentation and are the ideal starting point for benchmarking experiments. The provided yeast benchmark material enabled not only to test for the chemical space and coverage upon method implementation and developments but also allowed in-house routines for instrumental performance tests. Transferring the quality control strategy of proteomics workflows based on the number of protein identification in HeLa extracts, metabolite IDs in the yeast benchmarking material can be used as metabolomics quality control. Finally, the benchmark material opens new avenues for batch-to-batch corrections in large-scale non-targeted metabolomics studies. Full article
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Graphical abstract

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