Assays for the Impact of Functional Biomaterials on Cell Proliferation, Cell Viability, and Cell Metabolic Activities

A special issue of Journal of Functional Biomaterials (ISSN 2079-4983). This special issue belongs to the section "Antibacterial Biomaterials".

Deadline for manuscript submissions: closed (20 November 2022) | Viewed by 289

Special Issue Editors

Special Issue Information

Dear Colleagues,

Functional biomaterials have been synthesized and advocated for diverse applications as antimicrobial/antiviral agents, medical textiles, hygienic products, medical device coatings, scaffolds for tissue engineering, etc. As the first prerequisite for any intended application, “in vitro” toxicity testing by reliable methods must be conducted to assess the potentially harmful effects of functionalized biomaterials. This Special Issue invites review and research papers that cover traditional and emerging assays of cell proliferation, cell viability, and cell-cycle analysis after cells are exposed to functional biomaterials. As functional biomaterials bind to the cellular membrane or other components, improved methods to overcome possible interferences of functionalized biomaterials with common toxicity assays are also considered. The Special Issue will cover the following topics:

  • Novel fluorescent dyes beyond propidium iodide and 7-aminoactinomycin D that bind to double-stranded DNA or RNA of cells with compromised plasma membranes.
  • Enzymatic activity fluorogenic substrates: substrates that react with intracellular enzymes in live cells, e.g., esterases or extracellular glucose-6-phosphate dehydrogenase and lactate dehydrogenase released from damaged cells.
  • Chemiluminescent ATP assays and other biomarkers.
  • Combined substrates and techniques for probing plasma and intracellular cell membrane integrity (dead cells, stressed organelles such as endoplasmic reticulum (ER) and mitochondria), and live cells (esterases).
  • Metabolic activity agents: Novel agents that can determine the cellular redox potential in live cells. A classic example is the reduction of resazurin by live cells to a red and highly fluorescent resorufin.  
  • Assays using membrane integrity dyes for early apoptosis, e.g., the detection of phosphatidylserine expression on the cell membrane. In this context, this Special Issue also covers the topic of probing biomaterial–cell interactions by spectroscopy (FTIR, Raman, etc.) and spectroscopy (SEM, AFM, etc.)
  • Mitotoxicity agents can be used to detect changes in mitochondrial membrane potential.

The Special Issue also calls for papers related to emerging non-invasive and continuous methods for cell viability assays, e.g., cell-based impedance spectroscopy.

Prof. Dr. John H.T. Luong
Prof. Dr. Elisabeth Marion Schneider
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Functional Biomaterials is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • cytotoxicity
  • functional biomaterials
  • cytotoxic assays, viable cell assays

Published Papers

There is no accepted submissions to this special issue at this moment.
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