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New Advances in Genome Assembly

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: closed (31 December 2023) | Viewed by 831

Special Issue Editor


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Guest Editor
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 610054, China
Interests: bioinformatics; parallel computing; deep learning; protein classification; genome assembly
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In the past decade, affordable high-throughput DNA sequencing has accelerated the pace of genome assembly. However, genome assembly from high-throughput short-read sequencing is typically not as continuous as first generation genome assembly. Although early genome assembly projects often received the help of cloning maps or other map data, many current assembly projects abandoned these scaffold data and only assembled the genome into smaller fragments. Recently, new technologies have been developed that can perform chromosome scale assembly at lower cost and faster speed compared to traditional methods. This open-access Special Issue will bring together original research and review articles on genome assembly. It highlights new discoveries, approaches, and technical developments in genome assembly research. The main feature of this Special Issue is to provide an open access platform to introduce significant works in the field of genome assembly for chromosome scale assembly and recent genome projects that use these technologies to create highly continuous genome assemblies at low costs.

Topics of interest to this Special Issue include, but are not limited to, the following:

  • Genome contig assembly;
  • Chromosome-scale assembly;
  • Long-rang genome scaffolding;
  • De novo short read assembly;
  • Genome assembly algorithm and applications;
  • High-throughput technologies for genome assembly.

Prof. Dr. Quan Zou
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • assembly
  • comparative genomics
  • genome
  • high-throughput sequencing
  • genome scaffolding
  • de novo genome assembly
  • algorithm
  • bioinformatics
  • genome sequencing

Published Papers (1 paper)

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14 pages, 5639 KiB  
Brief Report
Selection of Catechin Biosynthesis-Related Genes and Functional Analysis from Chromosome-Level Genome Assembly in C. sinensis L. Variety ‘Sangmok’
by Dong-Jun Lee, Jin-Hyun Kim, Tae-Ho Lee, Myung-Eun Park, Byung-Ohg Ahn, So-Jin Lee, Jeong-Yong Cho and Chang-Kug Kim
Int. J. Mol. Sci. 2024, 25(7), 3634; https://doi.org/10.3390/ijms25073634 - 24 Mar 2024
Viewed by 590
Abstract
Camellia is an important plant genus that includes well-known species such as C. sinensis, C. oleifera, and C. japonica. The C. sinensis cultivar ‘Sangmok’, one of Korea’s standard types of tea landraces, is a small evergreen tree or shrub. Genome [...] Read more.
Camellia is an important plant genus that includes well-known species such as C. sinensis, C. oleifera, and C. japonica. The C. sinensis cultivar ‘Sangmok’, one of Korea’s standard types of tea landraces, is a small evergreen tree or shrub. Genome annotation has shown that Korean tea plants have special and unique benefits and superior components, such as catechin. The genome of Camellia sinensis cultivar ‘Sangmok’ was assembled on the chromosome level, with a length of 2678.62 Mbp and GC content of 38.16%. Further, 15 chromosome-scale scaffolds comprising 82.43% of the assembly (BUSCO completeness, 94.3%) were identified. Analysis of 68,151 protein-coding genes showed an average of 5.003 exons per gene. Among 82,481 coding sequences, the majority (99.06%) were annotated by Uniprot/Swiss-Prot. Further analysis revealed that ‘Sangmok’ is closely related to C. sinensis, with a divergence time of 60 million years ago. A total of 3336 exclusive gene families in ‘Sangmok’ were revealed by gene ontology analysis to play roles in auxin transport and cellular response mechanisms. By comparing these exclusive genes with 551 similar catechin genes, 17 ‘Sangmok’-specific catechin genes were identified by qRT-PCR, including those involved in phytoalexin biosynthesis and related to cytochrome P450. The ‘Sangmok’ genome exhibited distinctive genes compared to those of related species. This comprehensive genomic investigation enhances our understanding of the genetic architecture of ‘Sangmok’ and its specialized functions. The findings contribute valuable insights into the evolutionary and functional aspects of this plant species. Full article
(This article belongs to the Special Issue New Advances in Genome Assembly)
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