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Abiotic Stress in Plants, 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 20 February 2026 | Viewed by 429

Special Issue Editor


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Guest Editor
1. School of Mathematics and Statistics, Wuhan University of Technology, Wuhan 430070, China
2. Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
Interests: plant genomics; plant stress response; single-cell genomics; gene regulatory network; regulatory mechanism; multi-omics integration; next-generation sequencing; deep learning; bioinformatics; systems biology
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Special Issue Information

Dear Colleagues,

Plants are subjected to a multitude of environmental challenges, which ultimately impact their growth and development, and consequently affect crop yields. In the initial stages of environmental stress, an uncontrolled production of reactive oxygen species (ROS) results in oxidative stress, which ultimately leads to cell death. To cope with the environmental constraints, plants develop a highly sensitive defence mechanism, which enables them to sense and respond to these constraints via enzymatic and non-enzymatic defence mechanisms, in close collaboration with phytohormones. This Special Issue aims to foster a deeper understanding of the mechanisms underlying plant responses to abiotic stress. Further research in this field is thus required at the molecular, cellular, physiological, epigenetic, hormonal signalling, morphological, yield and final ecological levels. This Special Issue of the International Journal of Molecular Sciences (IJMS) welcomes the latest research contributions on plant responses to abiotic stress and proposals for novel solutions to enhance the adaptability of plants to environmental stress. We particularly welcome bioinformatics studies that integrate multi-omics big data, advanced statistical analysis, and artificial intelligence approaches to decode complex stress-response networks and to develop innovative strategies for improving plant resilience under abiotic stress.

Dr. Xiujun Zhang
Guest Editor

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Keywords

  • abiotic stress
  • ROS
  • redox
  • antioxidants
  • phytohormones
  • ethylene
  • plant stress eco-physiology

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Published Papers (1 paper)

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Research

19 pages, 5588 KB  
Article
Genome-Wide Identification and Functional Divergence of the Chloride Channel (CLC) Gene Family in Autotetraploid Alfalfa (Medicago sativa L.)
by Yanjun Fang, Guangzhi Jiang, Pingping Du, Jiayin Wang, Huan He, Hongfei Li, Hongbin Li, Fei Wang and Quanliang Xie
Int. J. Mol. Sci. 2025, 26(23), 11442; https://doi.org/10.3390/ijms262311442 - 26 Nov 2025
Viewed by 290
Abstract
Chloride channel proteins (CLCs) are essential anion transporters involved in plant growth, osmotic regulation, and ion homeostasis. However, their genome-wide characterization in tetraploid alfalfa (Medicago sativa L.) remains unexplored. In this study, a total of 35 CLC family members were identified and [...] Read more.
Chloride channel proteins (CLCs) are essential anion transporters involved in plant growth, osmotic regulation, and ion homeostasis. However, their genome-wide characterization in tetraploid alfalfa (Medicago sativa L.) remains unexplored. In this study, a total of 35 CLC family members were identified and underwent comprehensive bioinformatic analyses. Phylogenetic and structural analyses divided them into six subfamilies and two subclasses based on conserved residues such as GxGIPE. Members within the same subclass shared conserved domains and similar motif patterns. Analysis of duplication events indicated that 48 segmental duplications were the primary driving force behind the expansion of this gene family. Promoter analysis revealed abundant light, hormone, and stress-responsive cis-elements, suggesting multiple regulatory functions. Gene expression profiling demonstrated that salt, drought stress, and ABA treatment significantly induced the expression levels of some genes. Among them, MsCLC2 and MsCLC18 from Group c exhibited more than fivefold upregulation under both salt and drought stress, significantly higher than other members. Subcellular localization confirmed MsCLC18 on the plasma membrane, potentially regulating Cl efflux through a Cl/H+ antiporter mechanism to alleviate Cl toxicity. These findings provide a theoretical foundation for the function study of CLC genes in alfalfa and offer new insights into the molecular evolution of polyploid plants under abiotic stress. Full article
(This article belongs to the Special Issue Abiotic Stress in Plants, 2nd Edition)
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