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Conversion and Valorization of Lignocellulosic Biomass

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: 20 June 2026 | Viewed by 1589

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Research Institute on Terrestrial Ecosystems-IRET-CNR, Via Pietro Castellino 111, 80131 Naples, Italy
Interests: protein biochemistry; enzyme and protein purification; enzyme characterization; extremozymes; extremophilic microorganisms; biotechnology for sustainability; biocatalysts; biorefinery; biomass and waste valorization; bioresurces; bioactive molecules for well-being
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Special Issue Information

Dear Colleagues,

Lignocellulosic biomass is the most abundant renewable raw material on Earth. The valorization of lignocellulose, vegetal biomass and agro-industrial processing waste in order to produce high value-added products enhances energy production and promotes a lignocellulosic biorefinery approach. Many catalytic processes are employed to produce chemicals, bioactive molecules, biomaterials and biofuels from this biomass, as well as to develop economically feasible bioprocesses. Effective processes, advanced technologies and methods should be implemented as part of a green biorefinery and One Health approach, promoting a circular economy model for bioeconomy purposes. The sustainability, environmental impact and cost-effectiveness of technologies are important indicators in this context.

This Special Issue will collect original research articles, review, mini-review articles and short communications that highlight recent advances in green technologies for the conversion and valorization of lignocellulosic biomass (vegetal and agro-industrial waste) to value-added products. The scope of this Special Issue includes, but is not limited to, the following topics:

  • Biomass pre(treatment) for component isolation and valorization;
  • The innovative and sustainable catalytic conversion of lignocellulosic biomass streams;
  • Value-added products (bioactive molecules, chemicals, polymers and bioenergy) from lignocellulosic biomass and waste.

Dr. Loredana Marcolongo
Guest Editor

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Keywords

  • lignocellulosic biomass
  • vegetal and agro processing wast
  • catalytic and biocatalytic conversion
  • value added products
  • lignocellulosic platform
  • bioprocessing
  • biorefinery
  • green chemistry
  • circular economy, bioeconomy

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Published Papers (2 papers)

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Research

13 pages, 6175 KB  
Article
A Consolidated Saccharification, Fermentation, and Transesterification Process (cSFT) Converting Castor Oil to Biodiesel with Cellulose-Derived Ethanol
by Ester Korkus Hamal, Gilad Alfassi, Dmitry M. Rein and Yachin Cohen
Int. J. Mol. Sci. 2025, 26(24), 11902; https://doi.org/10.3390/ijms262411902 - 10 Dec 2025
Viewed by 116
Abstract
Environmental and economic concerns due to the increasing use of fossil-based chemicals, especially fuel, may be alleviated by production of renewable fuels based on plant biomass, in particular, waste. Multistep cascades of enzymatic reactions are being increasingly sought to enhance the effectiveness of [...] Read more.
Environmental and economic concerns due to the increasing use of fossil-based chemicals, especially fuel, may be alleviated by production of renewable fuels based on plant biomass, in particular, waste. Multistep cascades of enzymatic reactions are being increasingly sought to enhance the effectiveness of sustainable, environment-friendly processes. The biochemical transformation of lignocellulosic biomass and oils into fatty acid esters (“biodiesel”) involves biomass pretreatment, followed by polysaccharide hydrolysis and sugar fermentation to alcohol, either sequentially or simultaneously. Subsequent trans-esterification with waste or non-food-based oils is usually carried out in an organic solvent. Biocatalysis in aqueous emulsion offers significant advantages. This study presents a novel “one-pot” emulsion-based process for transforming unmodified cellulose and castor oil into biodiesel via hybridized yeasts with cellulose-coated micro-particles incorporating cellulolytic enzymes and lipases. The resultant consolidated bioprocess of saccharification, fermentation, and transesterification (cSFT) promotes effective substrate channeling and can potentially serve as a model for emulsion-based “one-pot” transformations of cellulose into valuable chemicals. Full article
(This article belongs to the Special Issue Conversion and Valorization of Lignocellulosic Biomass)
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19 pages, 1574 KB  
Article
Cellulase Production by Ultraviolet-Derived Mutant Trichoderma sp. Mut-4 Under Submerged Fermentation: Parameter Optimization and Large-Scale Application
by Iksu Ha, Seungjun Kim, Yun-Yeong Lee, Junseo Lee and Jeonghee Yun
Int. J. Mol. Sci. 2025, 26(16), 8000; https://doi.org/10.3390/ijms26168000 - 19 Aug 2025
Cited by 1 | Viewed by 1154
Abstract
This study aimed to optimize the parameters, including medium formulations and culture conditions, for submerged fermentation (SmF) systems using a mutant strain of Trichoderma sp., Mut-4. Optimization was performed using the one-factor-at-a-time (OFAT) method to enhance cellulase activity and productivity. Parameters such as [...] Read more.
This study aimed to optimize the parameters, including medium formulations and culture conditions, for submerged fermentation (SmF) systems using a mutant strain of Trichoderma sp., Mut-4. Optimization was performed using the one-factor-at-a-time (OFAT) method to enhance cellulase activity and productivity. Parameters such as the blending ratio of carbon sources, type of nitrogen source, and initial pH were evaluated for their effects on enzyme activity and productivity. The optimal conditions were determined to be as follows: a 3:1 Avicel-to-cellulose ratio, yeast extract as the nitrogen source, and an initial pH of 5.5. Under these conditions, cellulase production was initiated earlier, and the activity of all cellulase components, along with protein concentration, increased by 1.17- to 1.43-fold at the flask scale and by 1.3- to 2.0-fold at the reactor scale. These results demonstrate the superior activity and productivity of Mut-4 under optimized conditions, highlighting its potential for application in large-scale cellulase production. Full article
(This article belongs to the Special Issue Conversion and Valorization of Lignocellulosic Biomass)
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