Investigations on Nuclear DNA Content and DNA Synthesis in Plants and Fungi Using Flow Cytometry and Fluorescence Microscopy

Dear Colleagues,

Knowledge on nuclear DNA content and DNA synthesis activity during the cell cycle and endoreduplication is important not only for fundamental research but also in plant breeding and seed production and technology. Flow cytometry has become the method of choice for genome size estimation, ploidy analysis, establishment of cell cycle activity, and endoreplication intensity in plants. This method, however, does not provide information on the localization of nuclei of different ploidies in intact tissues; to elucidate this, fluorescence (confocal) microscopy is the technique used. The aim of this Special Issue is to provide insights into current research involving flow cytometry (including nuclei and chromosome sorting) and/or fluorescence microscopy to evaluate plant systematics, evolution, genome stability in in vitro cultures, responses to environmental stresses, and DNA synthesis during plant growth and development. Additionally, contributions on nucleoplasmic ratio and the relationship between nuclear and organellar genomes are welcome. Papers on applications of fungi flow cytometry and confocal microscopy in medical mycology, plant pathology, fermentation industry and environmental sciences as well as in fundamental studies on fungal biology and physiology will also be accepted.

In this Special Issue, we would like to include original articles, short communications, and reviews of recent applications of nuclear flow cytometry and fluorescence microscopy in fundamental plant research, as well as in agricultural and horticultural practices.

Prof. Elwira Sliwinska
Guest Editor

Dr. Monika Rewers
Dr. Iwona Jedrzejczyk
co-Guest Editors

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