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Legume Genetics and Breeding
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Legume Genetics and Breeding

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Plant Genetics and Genomics".

Deadline for manuscript submissions: closed (20 October 2022) | Viewed by 1843

Special Issue Editor

Dr. Sujan Mamidi

E-Mail Website
Guest Editor
HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA
Interests: genomics; diversity; population genomics; quantitative genomics; association mapping; resequencing; adaptation; domestication; QTL mapping
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Legumes are the second most important food source after cereals. They are used as an inexpensive meat alternative and are a rich source of many essential amino acids, minerals, and vitamins. Their yield and quality are restricted due to various biotic and abiotic stresses. Whole-genome sequence, re-sequencing, and other omic data are available for legumes, helping to identify QTL and genes associated with important traits. It is imperative to identify region-specific problems and develop cultivars and resources for screening.

This Special Issue aims to provide an updated overview of Legume Genetics for Plant Breeding. Therefore, authors are invited to submit original research and review articles that address the progress and current standing of genetic and genomic research in legumes.

Dr. Sujan Mamidi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Legume
  • Genome sequencing
  • QTL mapping
  • Association Mapping
  • Mutants
  • Abiotic stress
  • Biotic stress
  • Disease resistance
  • Soybean
  • Common bean
  • Chickpea
  • Lentil
  • Peanut.

Published Papers (1 paper)

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Research

18 pages, 2804 KiB  
Article
Identification of Reference Genes for RT-qPCR Analysis in Gleditsia microphylla under Abiotic Stress and Hormone Treatment
by Jiaqi Yang, Fengying Han, Li Yang, Jin Wang, Feng Jin, An Luo and Fuyong Zhao
Genes 2022, 13(7), 1227; https://doi.org/10.3390/genes13071227 - 10 Jul 2022
Cited by 10 | Viewed by 1541
Abstract
Gleditsia microphylla is an important galactomannan gums source plant with characteristics of drought resistance, barren tolerance, and good adaptability. However, the underlying molecular mechanisms of the biological process are not yet fully understood. Real-time quantitative PCR (RT-qPCR) is an accurate and convenient method [...] Read more.
Gleditsia microphylla is an important galactomannan gums source plant with characteristics of drought resistance, barren tolerance, and good adaptability. However, the underlying molecular mechanisms of the biological process are not yet fully understood. Real-time quantitative PCR (RT-qPCR) is an accurate and convenient method to quantify the gene expression level and transcription abundance of suitable reference genes. This study aimed to screen the best internal reference genes in G. microphylla under abiotic stresses, hormone treatments, and different tissues. Based on the transcriptome data, twelve candidate reference genes were selected, and ultimately, nine of them were further evaluated by the geNorm, NormFinder, BestKeeper, and RefFinder algorithms. These results show that TATA-binding protein 1 (TBP1)and Eukaryotic translation initiation factor 4A1 (EIF4A1)were the two most stable reference genes, and glyceraldehyde-3-phosphate dehydrogenase A subunit, chloroplastic (GAPA)and glyceraldehyde-3-phosphate dehydrogenase B subunit, chloroplastic (GAPB)were the two most unstable reference genes across all samples under the given experimental conditions. Meanwhile, the most stable reference genes varied among the different groups and tissues. Therefore, this study suggests that it is better to use a specific reference gene for a particular case rather than using a common reference gene. Full article
(This article belongs to the Special Issue Legume Genetics and Breeding)
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