Forest Tree Diseases Genomics: Growing Resources and Applications—2nd Edition

A special issue of Forests (ISSN 1999-4907). This special issue belongs to the section "Forest Health".

Deadline for manuscript submissions: 30 April 2026 | Viewed by 311

Special Issue Editor


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Guest Editor
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
Interests: detection; fungi and oomycetes; phylogeny; whole genome sequencing
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

A large number of fungi and oomycetes are of Tree Pathology. On one hand, fungi and oomycetes are among the dominant causal agents of plant diseases; on the other hand, fungi or oomycetes are also biological control agents of pathogens. Our understanding of the diversity, host range and specificity of fungi and oomycetes has improved with the enrichment of knowledge surrounding taxonomy and phylogeny over the years; however, many unknowns remain.

Fungi and oomycetes genomes are easily obtained, and have been used as models for genome evolution and the reconstruction of the phylogenetic relationship using genome-scale data. In these models, groundbreaking comparative genomic studies making use of these features have already been published. These breakthroughs are leading the way in fungi and oomycetes research.

The aim of this research topic is to collect articles focusing on the biodiversity, molecular systematics, and taxonomy of fungi and oomycetes all over the world. Additionally, approaches to the diagnosis of fungal and oomycete plant diseases, including advances in the field of molecular diagnosis of fungi and oomycetes, are welcome. Research involving diagnostic techniques should demonstrate improvements in diagnostic accuracy and time.

For these above reasons, we sincerely encourage experts and researchers to contribute original research articles, reviews, and communications that address the following topics:

  1. Phylogeny and diversity of fungi and oomycetes.
  2. Morphological and molecular characterization of plant pathogens.
  3. Genomics, transcriptomics, proteomics, and epigenomics data analyses.
  4. Molecular diagnostics of fungi and oomycetes.

Dr. Tingting Dai
Guest Editor

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Forests is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • detection
  • fungi and oomycetes
  • phylogeny
  • taxonomy
  • whole genome sequencing

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Published Papers (1 paper)

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Research

16 pages, 3432 KB  
Article
Establishment of a Genetic Transformation System for Fusarium circinatum Causing Pine Pitch Canker Using the Polyethylene Glycol (PEG)-Mediated Method
by Fengqi Wang, Fangyi Ju, Xiaorui Zhang and Tingting Dai
Forests 2026, 17(3), 327; https://doi.org/10.3390/f17030327 - 6 Mar 2026
Viewed by 171
Abstract
Fusarium circinatum Nirenberg & O’Donnell (the causal agent of Pine Pitch Canker, PPC), one of the most devastating threats to pine forests and nurseries worldwide, induces canker disease on a wide range of pine species. However, its status as a quarantined pathogen and [...] Read more.
Fusarium circinatum Nirenberg & O’Donnell (the causal agent of Pine Pitch Canker, PPC), one of the most devastating threats to pine forests and nurseries worldwide, induces canker disease on a wide range of pine species. However, its status as a quarantined pathogen and the scarcity of reliable genetic manipulation tools have long impeded in-depth genomic research on this fungus, and the infection mechanisms of F. circinatum remain an urgent area for investigation. A key approach to expounding its pathogenicity is to perform gene editing on candidate genes, which requires an efficient transformation system. Protoplast-mediated transformation is a critical means for investigating plant-pathogen interactions. During the course of this study, we constructed a PEG/CaCl2-mediated protoplast transformation method for F. circinatum. Following systematic optimization of transformation conditions, strain A015-1 was selected as the model organism. The optimal enzymolysis system consisted of 5 mg/mL Lysing enzymes, 12.5 mg/mL Driselase, and 7.5 mg/mL Snailase, with incubation at 30 °C for 3 h under shaking at 80 rpm. All positive transformants exhibited strong green fluorescent signals. A total of 31 transformants were obtained after hygromycin B (HPH) selection, and PCR verification confirmed successful amplification of the gfp and hph gene fragments in 30 transformants, corresponding to a positive rate of 96%. Transformation efficiency was calculated as the number of PCR-positive transformants per microgram of plasmid DNA, yielding an efficiency of 1 transformant/μg plasmid DNA under optimal conditions. Furthermore, no significant differences were observed in vegetative growth, development, or pathogenicity between the transformants and the wild-type (WT) strain. In addition, Green fluorescent protein (GFP) was efficiently transformed into F. circinatum protoplasts and functionally expressed. Collectively, this study successfully established a stable transformation system for F. circinatum, providing a foundational platform for analyzing virulence-related functional genes involved in host infection and deciphering the molecular mechanisms underlying the pathogen’s colonization of pine hosts. Full article
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