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Chemosensors
Special Issues
Electrochemical Biosensors: Advances and Prospects
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Electrochemical Biosensors: Advances and Prospects

A special issue of Chemosensors (ISSN 2227-9040). This special issue belongs to the section "Electrochemical Devices and Sensors".

Deadline for manuscript submissions: closed (30 April 2025) | Viewed by 5646

Special Issue Editor

Dr. Wenwen Tu

E-Mail Website
Guest Editor
School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, China
Interests: electrochemical biosensing; cell analysis; nano sensors; photoelectrochemical biosensors; immunoassay; nucleic acid detection; electrochemiluminescence determination; cell monitoring; photothermal therapy

Special Issue Information

Dear Colleagues,

Biosensors are now increasingly used in clinical diagnostics, the biomonitoring of clinical biomarkers, and targeted drug delivery. The need-based alliance of nanotechnology, biotechnology, and material science has revolutionized the field of biosensing for its diverse application. Of all the sensors, electrochemical biosensors are especially important as these are simple to fabricate, easy to operate, have portability, miniaturization ability, and facilitates rapid, sensitive, selective, on-site analysis in an economical manner. The future trend of electrochemical biosensors needs to focus on further improvisations in the amplification of various orders of magnitude, a lower detection limit, and the multiplex determination of target analytes in a high-throughput manner to realize the true potential.

This Special Issue will present comprehensive research outlining progress on the application of new materials or novel technology to improve the performance of electrochemical biosensors. Potential topics include electrochemical, electrochemiluminescent, and photoelectrochemical biosensors. We aim to attract both academic and industrial researchers in order to foster the current knowledge of electrochemistry and to present new ideas for biosensing applications. I cordially invite you to submit an article to this Special Issue. Both review articles and original research papers are welcomed.

Dr. Wenwen Tu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Chemosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • electrochemistry
  • electrochemiluminescence
  • photoelectrochemistry
  • immunoassay
  • Cytosensor
  • biomarkers
  • nucleic acid assay
  • pharmaceutical analysis
  • drug delivery

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

20 pages, 4604 KiB  
Article
Graphene-Modified Electrode for Linear Sweep Voltammetric Sensing of Catechol
by Florina Pogăcean, Lidia Măgeruşan, Alexandru Turza and Stela Pruneanu
Chemosensors 2025, 13(2), 43; https://doi.org/10.3390/chemosensors13020043 - 1 Feb 2025
Viewed by 878
Abstract
A graphene sample (EGr) was obtained in a single-step synthesis by electrochemical exfoliation of graphite rods. A combination of 0.05 M ammonium sulfate and 0.05 M ammonium thiocyanate was employed, leading to a graphene sample composed of few-layer, multi-layer and graphene oxide flakes. [...] Read more.
A graphene sample (EGr) was obtained in a single-step synthesis by electrochemical exfoliation of graphite rods. A combination of 0.05 M ammonium sulfate and 0.05 M ammonium thiocyanate was employed, leading to a graphene sample composed of few-layer, multi-layer and graphene oxide flakes. Due to the mild exfoliation conditions, large sheets with linear sizes in the range of tens to hundreds of micrometers were produced. The LSV technique gave information about the effect of catechol concentration on the electrochemical signal of bare and graphene-modified electrodes. Based on the resulting calibration plots, the corresponding analytical parameters (linear range, sensitivity, limit of quantification and limit of detection) were calculated for each electrode. In the case of the EGr/GC electrode the linear range was from 6 × 10−7 to 1 × 10−4 M catechol. The detection limit was low (1.82 × 10−7 M) while the quantification limit was 6 × 10−7 M. The sensitivity was five times higher than that corresponding to bare GC, proving the excellent electro-catalytic properties of the graphene-modified electrode. The practical applicability of the graphene-modified electrode was tested in tap water, obtaining an excellent recovery of 102%. Full article
(This article belongs to the Special Issue Electrochemical Biosensors: Advances and Prospects)
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19 pages, 3472 KiB  
Article
Electrochemical DNA Sensor Based on Poly(proflavine) Deposited from Natural Deep Eutectic Solvents for DNA Damage Detection and Antioxidant Influence Assessment
by Anna Porfireva, Anastasia Goida, Vladimir Evtugyn, Milena Mozgovaya, Tatiana Krasnova and Gennady Evtugyn
Chemosensors 2024, 12(10), 215; https://doi.org/10.3390/chemosensors12100215 - 16 Oct 2024
Viewed by 1455
Abstract
Electrochemical DNA sensors for DNA damage detection based on electroactive polymer poly(proflavine) (PPFL) that was synthesized at screen-printed carbon electrodes (SPCEs) from phosphate buffer (PB) and two natural deep eutectic solvents (NADESs) consisting of citric or malonic acids, D-glucose, and a certain amount [...] Read more.
Electrochemical DNA sensors for DNA damage detection based on electroactive polymer poly(proflavine) (PPFL) that was synthesized at screen-printed carbon electrodes (SPCEs) from phosphate buffer (PB) and two natural deep eutectic solvents (NADESs) consisting of citric or malonic acids, D-glucose, and a certain amount of water (NADES1 and NADES2) were developed. Poly(proflavine) coatings obtained from the presented media (PPFLPB, PPFLNADES1, and PPFLNADES2) were electrochemically polymerized via the multiple cycling of the potential or potentiostatic accumulation and used for the discrimination of thermal and oxidative DNA damage. The electrochemical characteristics of the poly(proflavine) coatings and their morphology were assessed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). The working conditions for calf thymus DNA implementation and DNA damage detection were estimated for all types of poly(proflavine) coatings. The voltammetric approach made it possible to distinguish native and chemically oxidized DNA while the impedimetric approach allowed for the successful recognition of native, thermally denatured, and chemically oxidized DNA through changes in the charge transfer resistance. The influence of different concentrations of conventional antioxidants and pharmaceutical preparations on oxidative DNA damage was characterized. Full article
(This article belongs to the Special Issue Electrochemical Biosensors: Advances and Prospects)
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15 pages, 3546 KiB  
Article
Urea Biosensing through Integration of Urease to the PEDOT-Polyamine Conducting Channels of Organic Electrochemical Transistors: pH-Change-Based Mechanism and Urine Sensing
by Jael R. Neyra Recky, Marjorie Montero-Jimenez, Juliana Scotto, Omar Azzaroni and Waldemar A. Marmisollé
Chemosensors 2024, 12(7), 124; https://doi.org/10.3390/chemosensors12070124 - 3 Jul 2024
Cited by 3 | Viewed by 2031
Abstract
We present the construction of an organic electrochemical transistor (OECT) based on poly(3,4-ethylendioxythiophene, PEDOT) and polyallylamine (PAH) and its evaluation as a bioelectronic platform for urease integration and urea sensing. The OECT channel was fabricated in a one-step procedure using chemical polymerization. Then, [...] Read more.
We present the construction of an organic electrochemical transistor (OECT) based on poly(3,4-ethylendioxythiophene, PEDOT) and polyallylamine (PAH) and its evaluation as a bioelectronic platform for urease integration and urea sensing. The OECT channel was fabricated in a one-step procedure using chemical polymerization. Then, urease was immobilized on the surface by electrostatic interaction of the negatively charged enzyme at neutral pH with the positively charged surface of PEDOH-PAH channels. The real-time monitoring of the urease adsorption process was achieved by registering the changes on the drain–source current of the OECT upon continuous scan of the gate potential during enzyme deposition with high sensitivity. On the other hand, integrating urease enabled urea sensing through the transistor response changes resulting from local pH variation as a consequence of enzymatic catalysis. The response of direct enzyme adsorption is compared with layer-by-layer integration using polyethylenimine. Integrating a polyelectrolyte over the adsorbed enzyme resulted in a more stable response, allowing for the sensing of urine even from diluted urine samples. These results demonstrate the potential of integrating enzymes into the active channels of OECTs for the development of biosensors based on local pH changes. Full article
(This article belongs to the Special Issue Electrochemical Biosensors: Advances and Prospects)
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