Progress in Enzyme Sensing Technology

A special issue of Chemosensors (ISSN 2227-9040). This special issue belongs to the section "(Bio)chemical Sensing".

Deadline for manuscript submissions: 15 October 2025 | Viewed by 676

Special Issue Editor


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Guest Editor
Institute of Chemistry, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Interests: electrochemical biosensing; glycochemistry; glycobiology; peptide science

Special Issue Information

Dear Colleagues,

Enzymes are catalytic proteins that play a major role in metabolic activity, the regulation of cell processes, and the performance of crucial cell activities. Abnormal levels of enzymatic activity or expression can cause different diseases. Additionally, pathogens such as bacteria and viruses use enzymes to cause infection. Therefore, it is crucial to detect enzyme activity for disease and infection diagnostics using sensing tools. This Special Issue aims to discuss various advances in the sensing technologies, approaches, and techniques used to monitor enzyme activity. Focus is not limited to the type of enzyme used; we also invite the contribution of perspectives or reviews regarding progress in approaches to monitoring enzymatic activity.

Dr. Israel Alshanski
Guest Editor

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Keywords

  • enzyme biosensing
  • metabolic labelling
  • label-free detection
  • electrochemistry
  • sensor design

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Published Papers (1 paper)

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Research

15 pages, 2594 KiB  
Article
Eliminating Assay Background of a Low-Cost, Colorimetric Glutamine Biosensor by Engineering an Alternative Formulation of Cell-Free Protein Synthesis
by Joseph P. Talley, Tyler J. Free, Tyler P. Green, Dallin M. Chipman and Bradley C. Bundy
Chemosensors 2025, 13(6), 206; https://doi.org/10.3390/chemosensors13060206 - 5 Jun 2025
Viewed by 303
Abstract
Glutamine is an essential biomolecule that plays a pivotal role in many diseases, such as cancer, where it can serve as fuel for rapid proliferation. Treatments for these diseases can be monitored and optimized through the detection of glutamine, though standard glutamine detection [...] Read more.
Glutamine is an essential biomolecule that plays a pivotal role in many diseases, such as cancer, where it can serve as fuel for rapid proliferation. Treatments for these diseases can be monitored and optimized through the detection of glutamine, though standard glutamine detection procedures are costly and require complex instrumentation. Cell-free protein synthesis (CFPS) has recently enabled a paper-based, colorimetric glutamine sensor that carries the potential to increase test accessibility while dramatically reducing consumer cost to enable at-home, rapid treatment monitoring. Test sensitivity remained limited by residual assay background, thus motivating this work where CFPS reactions traditionally formulated with glutamate salts were compared to systems using alternative salts, including aspartate, acetate, citrate, and sulfate, to reduce the background generation of glutamine. This led to the discovery of a novel aspartate-based CFPS system that boasts a high signal strength and indetectable background noise over 225 min. Acetate-, citrate-, and sulfate-based systems also yielded zero background glutamine detection but at a lower signal response compared to the aspartate-based system. These findings mark crucial advancements in producing a cost-effective, simple glutamine monitor while simultaneously showcasing the adaptability of CFPS’s open reaction environment for solving complex challenges in next-generation biosensor development. Full article
(This article belongs to the Special Issue Progress in Enzyme Sensing Technology)
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